##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139884_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13632109
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27258122716008	33.0	31.0	34.0	30.0	34.0
2	32.44063842212529	34.0	31.0	34.0	31.0	34.0
3	32.55584994222097	34.0	31.0	34.0	31.0	34.0
4	36.09713662060654	37.0	35.0	37.0	35.0	37.0
5	35.92806659629849	37.0	35.0	37.0	35.0	37.0
6	35.96811029019794	37.0	35.0	37.0	35.0	37.0
7	35.939107881253	37.0	35.0	37.0	35.0	37.0
8	35.91960429600438	37.0	35.0	37.0	35.0	37.0
9	37.73953377280067	39.0	37.0	39.0	35.0	39.0
10-11	37.60856900425312	39.0	37.0	39.0	35.0	39.0
12-13	37.62429034274887	39.0	37.0	39.0	35.0	39.0
14-15	38.91374339069618	40.0	38.0	41.0	36.0	41.0
16-17	38.858855551991255	40.0	38.0	41.0	36.0	41.0
18-19	38.9188695234171	40.0	38.0	41.0	36.0	41.0
20-21	38.913352898768125	40.0	38.0	41.0	35.5	41.0
22-23	38.873818868340976	40.0	38.0	41.0	35.0	41.0
24-25	38.855225152230304	40.0	38.0	41.0	35.0	41.0
26-27	38.750456807944516	40.0	38.0	41.0	35.0	41.0
28-29	38.666133326465385	40.0	38.0	41.0	35.0	41.0
30-31	38.51669674260711	40.0	38.0	41.0	34.0	41.0
32-33	38.42738837189463	40.0	38.0	41.0	34.0	41.0
34-35	38.380095417203854	40.0	38.0	41.0	34.0	41.0
36-37	38.278400035534816	40.0	38.0	41.0	34.0	41.0
38-39	38.23870221577001	40.0	38.0	41.0	34.0	41.0
40-41	38.23466248715518	40.0	38.0	41.0	34.0	41.0
42-43	38.19534614579464	40.0	38.0	41.0	34.0	41.0
44-45	38.083580571092	40.0	38.0	41.0	33.0	41.0
46-47	38.022173666581054	40.0	37.0	41.0	33.0	41.0
48-49	37.99303281789976	40.0	37.0	41.0	33.0	41.0
50-51	37.90550928864183	40.0	37.0	41.0	33.0	41.0
52-53	37.762509019500186	39.5	37.0	41.0	33.0	41.0
54-55	37.57720755857831	39.0	36.5	41.0	32.5	41.0
56-57	37.44559580659056	39.0	36.0	40.5	32.0	41.0
58-59	37.2165732415604	39.0	35.5	40.0	32.0	41.0
60-61	37.042691625361996	39.0	35.0	40.0	31.5	41.0
62-63	36.821357724079306	38.5	35.0	40.0	31.0	41.0
64-65	36.50283554442409	38.0	35.0	40.0	31.0	41.0
66-67	36.42609198095613	37.5	35.0	40.0	31.0	41.0
68-69	36.187534793682346	37.0	35.0	40.0	31.0	41.0
70-71	35.87747314342629	36.5	34.0	39.0	31.0	41.0
72-73	35.56713540340111	36.0	34.0	39.0	30.5	40.5
74-75	35.16921147586336	35.5	34.0	38.5	30.0	40.0
76-77	33.11715017283204	34.5	31.0	36.0	27.5	39.0
78-79	34.34836412433393	35.0	33.5	37.0	29.5	39.0
80-81	34.38244408813696	35.0	34.0	37.0	30.0	39.0
82-83	34.10851412741421	35.0	34.0	36.0	30.0	38.5
84-85	33.86056688109668	35.0	33.0	36.0	29.5	37.0
86-87	33.66388271075543	35.0	33.0	35.5	29.0	37.0
88-89	33.438045397638916	35.0	33.0	35.0	29.0	36.5
90-91	33.2874379246165	35.0	33.0	35.0	29.0	36.0
92-93	33.22798196211875	35.0	33.0	35.0	29.0	36.0
94-95	33.206280769551	35.0	33.0	35.0	29.0	36.0
96-97	33.253298170417274	35.0	33.0	35.0	29.0	35.0
98-99	33.29246468286017	35.0	33.0	35.0	29.5	35.0
100-101	32.03812116694727	33.5	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	1.0
18	0.0
19	22.0
20	90.0
21	350.0
22	1382.0
23	4765.0
24	12148.0
25	26628.0
26	48195.0
27	78370.0
28	117022.0
29	161789.0
30	215488.0
31	281361.0
32	369047.0
33	500885.0
34	725224.0
35	1109956.0
36	1928135.0
37	3263434.0
38	3431912.0
39	1335871.0
40	20033.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.8445524459935	20.31372401731823	13.509061583941268	24.332661952747003
2	14.942207811262067	21.71337187124035	40.43102790574841	22.91339241174917
3	18.085598104063344	26.44920984667	30.72082579200374	24.74436625726291
4	12.43480001646115	18.064506143112098	38.98991475779552	30.510779082631235
5	12.882662867008642	38.112150552842024	35.99000565136534	13.015180928783995
6	29.818680293709505	38.341836908727764	18.804346414777054	13.035136382785673
7	26.109005175649692	32.728936580227334	23.677898607735195	17.484159636387776
8	24.579927327123162	36.48274074192531	21.6164176676047	17.320914263346822
9	25.778512319094137	16.116068775954385	21.10217142052319	37.003247484428286
10-11	24.10539740372366	26.609541941097042	29.444490230980914	19.840570424198383
12-13	24.894740006081978	24.236872608971552	29.329298536536452	21.53908884841002
14-15	22.002187776094498	25.5947062625971	26.75089942069121	25.652206540617193
16-17	21.76372450088923	28.65816747657211	27.404573371388768	22.173534651149893
18-19	21.80223177499534	27.9590267360685	28.94982720575371	21.288914283182446
20-21	22.570140375200236	27.240206184546032	28.713011105210185	21.476642335043543
22-23	22.2445193240673	27.239592257600087	28.58980619055831	21.926082227774305
24-25	21.988464844347096	27.31592693471907	28.736184535485656	21.959423685448183
26-27	22.003173453985468	27.515350265909277	28.577559522267	21.903916757838253
28-29	21.940287766903598	27.59580508938887	28.583433215918262	21.88047392778927
30-31	22.22006399020958	27.447263085640948	28.4602482302058	21.87242469394367
32-33	21.91552254219155	27.601207382760123	28.511139242851115	21.972130832197212
34-35	22.026264883716184	27.610436728751292	28.465231847126066	21.898066540406457
36-37	22.094572359266447	27.525429832137217	28.460771249737103	21.919226558859236
38-39	21.926669706266665	27.67074086367034	28.497416771441326	21.90517265862167
40-41	22.175002048001367	27.517585851933568	28.384867606407628	21.92254449365744
42-43	22.10492883686023	27.612616908527244	28.208006944168183	22.074447310444345
44-45	22.185496848976353	27.76075525174467	27.998546298741395	22.05520160053759
46-47	22.279768769513776	27.65651839284245	27.768355109907816	22.295357727735954
48-49	22.162323629079086	27.778652642354846	27.804628613929268	22.2543951146368
50-51	22.12543582540451	27.897283960007265	27.731080990013	22.24619922457522
52-53	22.33202932299769	27.77506092140072	27.63520425894011	22.25770549666148
54-55	22.26202615072008	27.701226793235733	27.67470413592327	22.36204292012091
56-57	22.31136794426183	27.8872031920613	27.553310833378074	22.248118030298798
58-59	22.259635204001917	27.970198339630613	27.56674889808067	22.2034175582868
60-61	22.349837604153024	27.9519329100188	27.511132401408684	22.187097084419495
62-63	22.295117291661022	27.998230109026707	27.50127975513626	22.205372844176015
64-65	22.27970129076677	27.925453936287465	27.482098432146866	22.3127463407989
66-67	22.34401752603047	27.952625393030427	27.352241900350073	22.35111518058903
68-69	22.316127064916483	27.904120467871458	27.447820556178538	22.33193191103352
70-71	22.309268407553322	27.64757073327409	27.519942380692342	22.523218478480246
72-73	22.390759154993116	27.70090995149519	27.462244254790804	22.446086638720892
74-75	22.20801310589763	27.826066724120917	27.470036000419828	22.495884169561624
76-77	22.347409490745505	27.743246133711878	27.400859956923874	22.508484418618746
78-79	22.314695400581854	27.786234966159846	27.38839552122507	22.51067411203323
80-81	22.328129955588288	27.73403390791246	27.401871519339387	22.535964617159866
82-83	22.347255518122413	27.671895823354347	27.520719814578037	22.460128843945203
84-85	22.519759366969936	27.60140852840991	27.44189378742402	22.436938317196137
86-87	22.261248488434926	27.647341440291246	27.5286465464322	22.562763524841625
88-89	22.412239291008998	27.59774234931814	27.480933635182502	22.509084724490364
90-91	22.445855334735157	27.582250714072636	27.5304042509937	22.441489700198503
92-93	22.462948368680827	27.51231379303838	27.54984194710481	22.474895891175986
94-95	22.34261832252784	27.506539312809952	27.63186431931832	22.518978045343882
96-97	22.313649243256712	27.541457289488168	27.637859433433814	22.507034033821302
98-99	22.555354681447547	28.16336948315229	27.674427981715038	21.606847853685128
100-101	26.728413812043307	35.089607754947174	28.341092391104933	9.840886041904586
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2224.0
1	2036.0
2	3120.0
3	4681.5
4	6405.5
5	10717.5
6	14492.0
7	17005.0
8	18697.5
9	18387.5
10	16655.0
11	14894.5
12	15966.5
13	21022.0
14	32649.0
15	48569.5
16	61805.5
17	69901.0
18	72475.0
19	70123.5
20	66182.0
21	63632.0
22	64108.5
23	70296.0
24	83458.0
25	104938.5
26	133804.5
27	164077.0
28	194552.5
29	227237.5
30	256385.0
31	283201.5
32	312935.5
33	342118.5
34	369609.5
35	394861.0
36	424070.0
37	450154.5
38	467002.5
39	482263.5
40	500591.5
41	517516.5
42	529075.0
43	541873.5
44	554089.0
45	564078.0
46	570408.0
47	574838.0
48	579118.5
49	576180.0
50	565486.0
51	552096.0
52	533431.0
53	511595.5
54	486468.5
55	452124.5
56	416562.5
57	380001.5
58	339076.0
59	296286.5
60	250944.5
61	206997.0
62	165835.5
63	129090.0
64	98293.0
65	74245.0
66	56987.5
67	44594.0
68	34536.0
69	25182.5
70	18085.5
71	13250.5
72	9557.5
73	6874.5
74	4927.0
75	3476.0
76	2421.5
77	1580.0
78	1055.5
79	774.5
80	559.0
81	379.0
82	269.5
83	185.5
84	132.5
85	105.5
86	83.5
87	63.5
88	41.5
89	25.0
90	14.0
91	9.5
92	8.0
93	4.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0037338316470327516
3	5.868497677065229E-5
4	8.802746515597844E-5
5	3.667811048165768E-5
6	0.0
7	2.9342488385326146E-5
8	4.6214419206888684E-4
9	1.393768198302992E-4
10-11	6.235278781881806E-5
12-13	2.2373647393811183E-4
14-15	7.3356220963315365E-6
16-17	4.768154362615499E-4
18-19	0.0
20-21	4.768339050327325E-5
22-23	0.0
24-25	0.0
26-27	7.341194725116673E-5
28-29	0.0
30-31	1.0670542796529969E-4
32-33	4.391080718764564E-4
34-35	0.0
36-37	3.68583354716132E-4
38-39	0.0011142324626563303
40-41	0.0
42-43	0.0
44-45	3.1253167518174006E-4
46-47	0.0
48-49	1.1852152925800742E-5
50-51	1.3182451520535863E-4
52-53	5.973639941435725E-4
54-55	0.003283610898390231
56-57	0.003623346750033355
58-59	0.004319427422716295
60-61	9.557705343810326E-4
62-63	8.560950090816699E-6
64-65	5.111149526549455E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	1.4154902762895472E-4
74-75	1.8502788578395136E-5
76-77	0.0
78-79	0.0
80-81	4.72360226921853E-4
82-83	0.0
84-85	0.0
86-87	1.0069128088522537E-5
88-89	0.0
90-91	0.0010406648641310555
92-93	1.2721391974709873E-4
94-95	5.404502123212704E-6
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	334.0
20-21	1008.0
22-23	2303.0
24-25	5044.0
26-27	8426.0
28-29	17575.0
30-31	36855.0
32-33	45817.0
34-35	62732.0
36-37	95726.0
38-39	114090.0
40-41	114837.0
42-43	131596.0
44-45	153005.0
46-47	150669.0
48-49	142098.0
50-51	130721.0
52-53	127481.0
54-55	139407.0
56-57	146865.0
58-59	145587.0
60-61	144709.0
62-63	137438.0
64-65	141721.0
66-67	152053.0
68-69	150447.0
70-71	147777.0
72-73	141761.0
74-75	143206.0
76-77	140096.0
78-79	149245.0
80-81	152324.0
82-83	145465.0
84-85	145402.0
86-87	150589.0
88-89	162302.0
90-91	179532.0
92-93	175897.0
94-95	196509.0
96-97	290620.0
98-99	1117774.0
100-101	7695066.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.79474489105509
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.38983501452444	73.1821384099
2	7.741311700929518	12.818798548011983
3	2.09684021070229	5.20822050967207
4	0.8069292256867975	2.672379975435001
5	0.37935555965908074	1.570432339248851
6	0.1998433717711527	0.9927588590376519
7	0.12269664763640765	0.7111046348030836
8	0.07213055676973978	0.4777624837280298
9	0.04698808159249476	0.35013296055336174
>10	0.14131382125891923	1.7533564298673057
>50	0.0020310633417599112	0.11302571098575848
>100	6.482717456687301E-4	0.09178700664934006
>500	6.118574399368665E-5	0.036216123886491855
>1k	1.5288637814906705E-5	0.02188600822109687
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	7.335622096331536E-6	4.401373257798922E-5	0.0	0.0	0.0
3	1.4671244192663071E-5	4.401373257798922E-5	0.0	7.335622096331536E-6	0.0
4	1.4671244192663071E-5	1.1003433144497304E-4	0.0	2.9342488385326143E-5	0.0
5	1.4671244192663071E-5	1.247055756376361E-4	0.0	2.9342488385326143E-5	0.0
6	1.4671244192663071E-5	1.247055756376361E-4	0.0	2.9342488385326143E-5	2.200686628899461E-5
7	1.4671244192663071E-5	1.3204119773396766E-4	0.0	5.1349354674320755E-5	2.200686628899461E-5
8	1.4671244192663071E-5	1.5404806402296225E-4	0.0	5.8684976770652286E-5	2.200686628899461E-5
9	1.4671244192663071E-5	3.5210986062391374E-4	0.0	8.069184305964689E-5	2.200686628899461E-5
10-11	3.667811048165768E-5	3.5210986062391374E-4	0.0	1.0269870934864151E-4	2.200686628899461E-5
12-13	3.667811048165768E-5	3.5210986062391374E-4	0.0	1.357090087821334E-4	2.200686628899461E-5
14-15	3.667811048165768E-5	4.4747294787622367E-4	0.0	1.4304463087846497E-4	2.200686628899461E-5
16-17	4.401373257798922E-5	4.621441920688868E-4	0.0	1.723871192637911E-4	2.200686628899461E-5
18-19	5.501716572248652E-5	5.06157924646876E-4	0.0	1.9072617450461993E-4	4.401373257798922E-5
20-21	6.968840991514958E-5	5.501716572248653E-4	0.0	2.2006866288994608E-4	4.401373257798922E-5
22-23	9.903089830047573E-5	6.308635002845121E-4	0.0	2.640823954679353E-4	6.235278781881805E-5
24-25	1.357090087821334E-4	6.858806660069986E-4	0.0	3.961235932019029E-4	1.0269870934864151E-4
26-27	1.6138368611929378E-4	7.262265875368221E-4	0.0	5.244969798877048E-4	1.0269870934864151E-4
28-29	1.723871192637911E-4	7.298943985849879E-4	0.0	0.0013827647651584946	1.0269870934864151E-4
30-31	1.7605493031195687E-4	8.582677852707897E-4	0.0	0.003304697754397357	1.0269870934864151E-4
32-33	1.833905524082884E-4	8.582677852707897E-4	0.0	0.00654337490992773	1.1003433144497304E-4
34-35	1.9806179660095146E-4	0.0010489939597754096	0.0	0.010798035725800022	1.1736995354130457E-4
36-37	2.0906522974544877E-4	0.0012470557563763612	0.0	0.017165355705415795	1.1736995354130457E-4
38-39	2.1273304079361455E-4	0.001298405111050682	0.0	0.0281687888499131	1.247055756376361E-4
40-41	2.3473990708260914E-4	0.0014524531750736442	0.0	0.03780412847344457	1.247055756376361E-4
42-43	2.5674677337160376E-4	0.001500134718699799	0.0	0.04540383296524404	1.2837338668580188E-4
44-45	2.640823954679353E-4	0.0016211724832892694	0.0	0.054918134824186046	1.3204119773396766E-4
46-47	2.640823954679353E-4	0.0017018643263489164	0.0	0.06460482380239184	1.3204119773396766E-4
48-49	2.860892617569299E-4	0.0017018643263489164	0.0	0.0741337969055265	1.4671244192663072E-4
50-51	2.860892617569299E-4	0.0018925905008535361	0.0	0.08308692367409914	1.5038025297479647E-4
52-53	2.897570728050957E-4	0.0019072617450461994	0.0	0.0915669028174584	1.723871192637911E-4
54-55	3.190995611904218E-4	0.0019219329892388626	0.0	0.10044300555401955	1.943939855527857E-4
56-57	3.374386164312507E-4	0.001943939855527857	0.0	0.10860021732514022	2.0172960764911724E-4
58-59	3.5944548272024524E-4	0.00195861109972052	0.0	0.11563507891552216	2.0539741869728302E-4
60-61	3.6311329376841105E-4	0.0019989570212503435	0.0	0.12234350532261735	2.0539741869728302E-4
62-63	3.7044891586474255E-4	0.002039302942780167	0.0	0.12929767506993967	2.3473990708260914E-4
64-65	3.777845379610741E-4	0.002274042849862776	0.0	0.1359474165002642	2.3473990708260914E-4
66-67	3.8512016005740567E-4	0.0022850462830072736	0.0	0.14282089440452683	2.3473990708260914E-4
68-69	4.071270263464003E-4	0.0023473990708260915	0.0	0.15018952680029185	2.3473990708260914E-4
70-71	4.1079483739456604E-4	0.002391412803404081	0.0	0.1568832819631944	2.494111512752722E-4
72-73	4.144626484427318E-4	0.0024097518586449094	0.0	0.1624436835122137	2.787536396605984E-4
74-75	4.217982705390633E-4	0.002475772457511893	0.0	0.16671301557227866	2.787536396605984E-4
76-77	4.2913389263539485E-4	0.0025601321116197062	0.0	0.17057888841704538	2.8242145070876413E-4
78-79	4.364695147317264E-4	0.002611481466294027	0.0	0.17412932951166982	2.860892617569299E-4
80-81	4.621441920688868E-4	0.002611481466294027	0.0	0.17560745736408062	2.897570728050957E-4
82-83	4.621441920688868E-4	0.002611481466294027	0.0	0.176278666785895	3.080961280459245E-4
84-85	4.621441920688868E-4	0.002629820521534856	0.0	0.17697555088504646	3.080961280459245E-4
86-87	4.694798141652183E-4	0.0026995089314500054	0.0	0.17724696890261074	3.1543175014225606E-4
88-89	4.7681543626154984E-4	0.002809543262894978	0.0	0.17731665731252588	3.301029943349191E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14095	0.0	46.351635	1
GTATCAA	24835	0.0	34.313778	1
TCAACGC	29580	0.0	28.052607	4
ATCAACG	29770	0.0	27.83172	3
CAACGCA	30300	0.0	27.22194	5
AACGCAG	31105	0.0	26.58403	6
TATCAAC	31645	0.0	26.536903	2
ACGCAGA	36710	0.0	22.53638	7
CGCAGAG	37545	0.0	22.024136	8
GTACATG	37310	0.0	21.907875	1
TACATGG	37260	0.0	21.492682	2
ACATGGG	37390	0.0	20.951973	3
CATGGGG	28400	0.0	19.57603	4
GCAGAGT	45530	0.0	18.143236	9
GAGTACT	26270	0.0	17.638693	12-13
AGAGTAC	39875	0.0	17.370953	10-11
CAGAGTA	43245	0.0	16.951288	10-11
GTGGTAT	7705	0.0	16.72192	1
ATGGGGG	18350	0.0	15.509976	5
CTTATAC	9430	0.0	15.46427	1
>>END_MODULE