FastQCFastQC Report
Fri 27 May 2016
SRR2139883_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139883_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19189796
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA211100.046.479361
GTATCAA368950.034.9918751
TCAACGC444350.028.3695474
ATCAACG447150.028.1731993
CAACGCA456500.027.5322975
TATCAAC473150.026.9249442
AACGCAG473500.026.6581466
ACGCAGA550450.022.9012187
CGCAGAG557600.022.6000888
GTACATG492900.021.2243251
TACATGG488250.020.7441442
ACATGGG492850.020.0008053
GCAGAGT671900.018.6500689
CATGGGG382150.018.3188844
GAGTACT416550.017.78598412-13
AGAGTAC601400.017.66154710-11
CAGAGTA656650.016.84778210-11
GTGGTAT110600.016.6056731
AGTACTT454900.015.62739812-13
GTACTTT476250.015.56621214-15
Produced by FastQC (version 0.11.5)