##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139883_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19189796
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.712472920504208	31.0	31.0	34.0	30.0	34.0
2	31.9143534407557	33.0	31.0	34.0	30.0	34.0
3	32.0192794128713	33.0	31.0	34.0	30.0	34.0
4	35.72367314378954	37.0	35.0	37.0	33.0	37.0
5	35.37401924439426	37.0	35.0	37.0	33.0	37.0
6	35.38564042056518	37.0	35.0	37.0	33.0	37.0
7	35.36103734505568	37.0	35.0	37.0	33.0	37.0
8	35.321476841129524	37.0	35.0	37.0	33.0	37.0
9	37.0805648481099	39.0	37.0	39.0	33.0	39.0
10-11	36.88042421086706	39.0	37.0	39.0	33.0	39.0
12-13	36.90397912515589	39.0	37.0	39.0	33.0	39.0
14-15	38.01730078318707	39.5	37.0	40.5	33.0	41.0
16-17	37.923754009683066	39.0	37.0	40.0	33.0	41.0
18-19	37.98047214780188	39.5	37.0	40.5	33.0	41.0
20-21	37.97655259761424	40.0	37.0	41.0	33.0	41.0
22-23	37.92737764076979	39.0	37.0	40.0	33.0	41.0
24-25	37.892032070876446	39.0	37.0	41.0	33.0	41.0
26-27	37.749656062157044	39.0	37.0	40.5	33.0	41.0
28-29	37.63797457638194	39.0	37.0	40.0	33.0	41.0
30-31	37.46236715357996	39.0	36.5	40.0	32.0	41.0
32-33	37.34632406381003	39.0	36.0	40.0	32.0	41.0
34-35	37.2742473538469	39.0	36.0	40.0	32.0	41.0
36-37	37.15966745727725	39.0	36.0	40.0	31.0	41.0
38-39	37.109365270704686	39.0	36.0	40.0	31.0	41.0
40-41	37.09168821830602	39.0	36.0	40.0	31.0	41.0
42-43	37.0429739857103	39.0	36.0	40.0	31.0	41.0
44-45	36.911713182979554	39.0	35.5	40.0	31.0	41.0
46-47	36.83977428794931	39.0	35.0	40.0	31.0	41.0
48-49	36.814717540857444	39.0	35.0	40.0	31.0	41.0
50-51	36.71366104573643	38.5	35.0	40.0	30.5	41.0
52-53	36.54590224507679	38.0	35.0	40.0	30.0	41.0
54-55	36.33980878890897	38.0	35.0	40.0	30.0	41.0
56-57	36.197583091182466	38.0	34.5	40.0	30.0	41.0
58-59	35.96105652643504	38.0	34.0	40.0	29.0	41.0
60-61	35.78363931432129	38.0	34.0	40.0	29.0	41.0
62-63	35.56124858871935	37.5	34.0	40.0	28.5	41.0
64-65	35.248350195752984	37.0	34.0	39.5	28.0	41.0
66-67	35.21803226063582	36.5	34.0	39.0	28.0	40.0
68-69	35.00886239759916	36.0	33.0	39.0	28.0	40.0
70-71	34.72370469769588	36.0	33.0	39.0	28.0	40.0
72-73	34.435295761826126	35.0	33.0	38.5	28.0	40.0
74-75	34.03718613721459	35.0	32.5	37.5	27.5	39.5
76-77	32.00419558266913	33.0	30.0	35.5	25.0	38.5
78-79	33.29479154900453	34.5	32.0	36.0	26.5	39.0
80-81	33.394541363550694	35.0	32.0	36.0	27.0	39.0
82-83	33.12470735317768	34.5	32.0	36.0	27.0	37.5
84-85	32.877008735739324	34.0	32.0	35.5	27.0	37.0
86-87	32.67309382981435	34.0	32.0	35.0	26.5	37.0
88-89	32.4551365314304	34.0	31.0	35.0	26.0	36.0
90-91	32.328424006813705	34.0	31.0	35.0	26.0	36.0
92-93	32.27758500454587	34.0	31.0	35.0	26.0	36.0
94-95	32.29053459626962	34.0	31.0	35.0	27.0	35.0
96-97	32.40271117927766	34.0	31.0	35.0	27.0	35.0
98-99	32.527591920199576	34.0	31.5	35.0	28.0	35.0
100-101	31.31265808303806	32.5	29.5	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	0.0
15	1.0
16	0.0
17	2.0
18	14.0
19	66.0
20	302.0
21	1272.0
22	4582.0
23	13946.0
24	33797.0
25	69171.0
26	122551.0
27	195480.0
28	285693.0
29	392607.0
30	518925.0
31	674938.0
32	882041.0
33	1176969.0
34	1625475.0
35	2306539.0
36	3307144.0
37	4030280.0
38	2885512.0
39	659159.0
40	3328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.34652603915122	21.0153302307122	13.895238907177543	23.742904822959034
2	15.152434374037721	22.32445073655235	40.248087089008344	22.275027800401585
3	18.26799137812302	26.970504990961597	30.84881756646725	23.91268606444813
4	12.672753305507428	18.567371652595295	39.11850633920368	29.641368702693597
5	12.711880923332764	38.38880867261275	36.10392152326018	12.795388880794306
6	29.416545126378622	38.89542129577615	18.841018424583563	12.84701515326166
7	25.75067633604215	32.94061244531273	24.02131966495734	17.28739155368778
8	24.245099916752324	36.94458362483047	21.70677797623468	17.103538482182522
9	25.583004490672767	16.228173597658127	21.4293758003937	36.75944611127541
10-11	23.882588473956627	26.917763098498416	29.581951293827647	19.61769713371731
12-13	24.70950591493772	24.56406899251619	29.53755420370627	21.188870888839816
14-15	21.775829855812162	25.921244232188105	27.042011836030866	25.26091407596887
16-17	21.468253214501967	28.99388107102896	27.675161608058318	21.862704106410753
18-19	21.467175284277427	28.26716525811404	29.32878171223629	20.93687774537224
20-21	22.219227393634554	27.533357899960876	29.161655236201554	21.085759470203012
22-23	21.944583832775006	27.492241416042397	29.002542608200272	21.560632142982328
24-25	21.698951198072155	27.61265913728794	29.09487653271797	21.59351313192193
26-27	21.69472875074945	27.75574715274418	28.99253989541054	21.556984201095826
28-29	21.62513268010337	27.913964663433568	28.949666946901804	21.511235709561262
30-31	21.94029013706284	27.69012114034839	28.869331910331542	21.50025681225723
32-33	21.725791550025935	27.870489179609475	28.825700607218614	21.578018663145976
34-35	21.810976690748003	27.945706787085616	28.818326819817152	21.424989702349233
36-37	21.85986458544059	27.863746969183822	28.737877990728183	21.5385104546474
38-39	21.69332596201293	27.964939673882412	28.800099914142603	21.541634449962054
40-41	21.913314018837816	27.84523333199507	28.655389230477812	21.586063418689307
42-43	21.844651792921336	27.96743928837498	28.44697493114549	21.74093398755819
44-45	21.946111686319266	28.083429482550805	28.241731586050257	21.728727245079675
46-47	22.00307593400657	28.04646979370604	28.01114588291796	21.939308389369426
48-49	21.925246620934114	28.145931729097505	28.029255751063246	21.89956589890513
50-51	21.94821366662022	28.24355063178668	27.915088308455992	21.89314739313711
52-53	22.09334582205958	28.13220330871482	27.81309983061595	21.96135103860965
54-55	22.040117939749177	28.05758161023301	27.887023500169132	22.015276949848676
56-57	22.064415906774222	28.177471522501747	27.804260229551076	21.953852341172958
58-59	22.037527316269315	28.273914778593706	27.784129649811064	21.904428255325918
60-61	22.101045524589296	28.231592173127712	27.77629003734911	21.891072264933882
62-63	22.062993546201152	28.25875422254815	27.731284504097914	21.946967727152785
64-65	22.010956472007003	28.21668782135457	27.740960055028985	22.031395651609447
66-67	22.108583911761144	28.228416466051577	27.608639031921705	22.054360590265574
68-69	22.03896690786511	28.2291340973322	27.70828595088668	22.023613043916015
70-71	22.04177944603823	28.004354190153467	27.75336215246094	22.200504211347354
72-73	22.09883833696403	28.033059387958907	27.73950023002435	22.128602045052716
74-75	21.964649804173995	28.14567317351826	27.72363170709958	22.16604531520816
76-77	22.022384182879335	28.118839403203044	27.669671382483074	22.18910503143455
78-79	22.05677401086442	28.099319524503212	27.658358666387777	22.18554779824459
80-81	22.037942244090793	28.09657778221679	27.664390476123707	22.20108949756871
82-83	22.087188958056124	28.020286076023716	27.779424944262843	22.113100021657317
84-85	22.2276640648344	27.99321233218433	27.66614203642114	22.112981566560133
86-87	22.01107382472965	28.01372957045344	27.78961045456456	22.185586150252355
88-89	22.125855930183736	27.97290229499137	27.71380113578185	22.187440639043047
90-91	22.15990224188508	27.970429642498278	27.76591789937834	22.1037502162383
92-93	22.15802301229191	27.925862885739573	27.794160371275943	22.121953730692574
94-95	21.946784338824852	27.95419600924507	27.892248569626304	22.206771082303774
96-97	21.92417993018675	27.997076478397396	27.944541104440173	22.134202486975678
98-99	22.079601994219846	28.689327671644392	27.952902984624256	21.278167349511502
100-101	22.920533410730055	31.21535147936594	25.112676883609492	20.751438226294518
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3371.0
1	3159.5
2	5076.5
3	7630.0
4	10190.0
5	16819.0
6	22695.5
7	26789.5
8	29841.0
9	29682.5
10	26785.5
11	23319.5
12	24218.5
13	31544.5
14	49788.0
15	75296.5
16	96119.5
17	108642.0
18	113229.5
19	110768.5
20	105280.5
21	100526.0
22	100135.5
23	108839.0
24	128249.5
25	158057.5
26	196867.0
27	239251.5
28	284818.0
29	333454.5
30	378866.0
31	422388.0
32	465892.5
33	505668.0
34	543929.5
35	579464.5
36	619294.5
37	657179.0
38	683233.0
39	703894.5
40	725868.0
41	744788.0
42	754295.5
43	766550.5
44	779588.0
45	790186.0
46	795927.5
47	797502.5
48	795139.0
49	784200.0
50	770040.0
51	748904.5
52	714138.0
53	676581.0
54	638776.0
55	592237.0
56	543054.0
57	488537.5
58	431200.5
59	375849.0
60	317804.0
61	261617.0
62	209448.0
63	163189.0
64	124408.0
65	94437.5
66	72490.5
67	56618.0
68	44249.0
69	32650.0
70	23525.5
71	17263.5
72	12577.5
73	8970.5
74	6359.5
75	4534.0
76	3240.0
77	2264.0
78	1579.5
79	1158.0
80	863.0
81	628.5
82	463.0
83	323.0
84	225.0
85	154.0
86	117.5
87	99.5
88	76.0
89	52.0
90	36.0
91	27.0
92	21.5
93	16.5
94	10.0
95	6.0
96	5.0
97	3.5
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0016936084156392282
3	3.64777197214603E-5
4	2.0844411269405886E-5
5	4.168882253881177E-5
6	0.0
7	5.2111028173514716E-6
8	3.699883000319545E-4
9	1.094331591643809E-4
10-11	5.471657958219045E-5
12-13	1.745719443812743E-4
14-15	7.816654226027207E-6
16-17	4.247048796141449E-4
18-19	1.5633308452054413E-5
20-21	6.514269076470248E-5
22-23	0.0
24-25	0.0
26-27	2.6082542221571664E-5
28-29	2.6103998801095544E-6
30-31	4.967605460433755E-5
32-33	3.276782447099559E-4
34-35	0.0
36-37	3.3301930845950446E-4
38-39	9.95299313115735E-4
40-41	0.0
42-43	0.0
44-45	2.4032694952129055E-4
46-47	0.0
48-49	8.376879377320867E-6
50-51	1.974813454889308E-5
52-53	4.6433689003046766E-4
54-55	0.002554810686945726
56-57	0.0028821606095196747
58-59	0.0037888185493025603
60-61	7.588261750631626E-4
62-63	3.010139202382345E-6
64-65	4.323478258963316E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	8.976551899954534E-5
74-75	9.74541468493659E-6
76-77	0.0
78-79	0.0
80-81	3.251351520784112E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	7.655697377349471E-4
92-93	1.1281038649740897E-4
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	732.0
20-21	2158.0
22-23	4433.0
24-25	9630.0
26-27	13607.0
28-29	24127.0
30-31	47958.0
32-33	59771.0
34-35	81396.0
36-37	121301.0
38-39	145049.0
40-41	149072.0
42-43	172620.0
44-45	203994.0
46-47	199755.0
48-49	187028.0
50-51	173444.0
52-53	169579.0
54-55	184058.0
56-57	193792.0
58-59	194793.0
60-61	194020.0
62-63	188007.0
64-65	194348.0
66-67	207339.0
68-69	211072.0
70-71	209576.0
72-73	204033.0
74-75	209831.0
76-77	198777.0
78-79	215894.0
80-81	223515.0
82-83	218093.0
84-85	223487.0
86-87	236787.0
88-89	257935.0
90-91	290560.0
92-93	297115.0
94-95	350306.0
96-97	521213.0
98-99	1668491.0
100-101	1.05311E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.5113769899667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.58482649100846	72.20671187699381
2	7.298243889964788	11.897798181592817
3	2.0801267994052566	5.086619991997642
4	0.8456041516413694	2.7570543515488253
5	0.4087291701747364	1.6658038738454597
6	0.2413604891300203	1.1804175491975908
7	0.15429813606747553	0.8803937476491623
8	0.09515026028257768	0.6204662989269316
9	0.06287724463413626	0.46126897123170973
>10	0.2236849086120019	2.797329494897014
>50	0.003971123247590014	0.2151698084978793
>100	0.0010393664675537365	0.14329206835037409
>500	3.772451077772219E-5	0.02393735690702676
>1k	5.02448531534195E-5	0.06373642836374172
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.2111028173514716E-6
2	0.0	3.126661690410883E-5	0.0	0.0	5.2111028173514716E-6
3	0.0	3.64777197214603E-5	0.0	5.2111028173514716E-6	5.2111028173514716E-6
4	0.0	9.901095352967796E-5	0.0	2.0844411269405886E-5	1.0422205634702943E-5
5	0.0	1.0422205634702944E-4	0.0	2.605551408675736E-5	1.0422205634702943E-5
6	0.0	1.0422205634702944E-4	0.0	3.126661690410883E-5	1.0422205634702943E-5
7	0.0	1.1464426198173238E-4	0.0	4.168882253881177E-5	1.0422205634702943E-5
8	0.0	1.2506646761643533E-4	0.0	4.689992535616324E-5	1.5633308452054416E-5
9	0.0	2.5013293523287066E-4	0.0	8.337764507762355E-5	2.0844411269405886E-5
10-11	0.0	2.5013293523287066E-4	0.0	1.094331591643809E-4	2.0844411269405886E-5
12-13	0.0	2.6055514086757356E-4	0.0	1.4069977606848973E-4	2.0844411269405886E-5
14-15	0.0	3.5956609439725154E-4	0.0	1.459108788858412E-4	5.211102817351471E-5
16-17	0.0	3.830160570753332E-4	0.0	1.6414973874657134E-4	6.253323380821767E-5
18-19	0.0	4.168882253881177E-4	0.0	1.7978304719862577E-4	6.774433662556913E-5
20-21	0.0	4.6378815074428096E-4	0.0	1.9802190705935592E-4	6.774433662556913E-5
22-23	0.0	5.393491415958773E-4	0.0	2.26682972554789E-4	8.337764507762355E-5
24-25	0.0	6.044879268127708E-4	0.0	2.5794958945889786E-4	9.901095352967796E-5
26-27	0.0	6.461767493515825E-4	0.0	3.361161317191699E-4	9.901095352967796E-5
28-29	0.0	6.487823007602582E-4	0.0	0.0011255982085479179	9.901095352967796E-5
30-31	0.0	7.191321887945031E-4	0.0	0.0028426565868652277	9.901095352967796E-5
32-33	0.0	7.321599458378818E-4	0.0	0.005732213099086619	1.0422205634702944E-4
34-35	5.2111028173514716E-6	8.650430676803443E-4	0.0	0.009601456940970086	1.1203871057305664E-4
36-37	5.2111028173514716E-6	0.0010083483951575096	0.0	0.01577661377953158	1.1464426198173238E-4
38-39	5.2111028173514716E-6	0.0010604594233310245	0.0	0.025385887374727693	1.1985536479908384E-4
40-41	1.0422205634702943E-5	0.0011881314423561355	0.0	0.03495607769879368	1.1985536479908384E-4
42-43	1.302775704337868E-5	0.001232425816303623	0.0	0.04272322644805604	1.1985536479908384E-4
44-45	2.605551408675736E-5	0.00138094224659814	0.0	0.051970328397446225	1.2506646761643533E-4
46-47	2.605551408675736E-5	0.0014981920599885482	0.0	0.062376900723697115	1.2767201902511106E-4
48-49	3.126661690410883E-5	0.001508614265623251	0.0	0.0718819522625462	1.459108788858412E-4
50-51	3.126661690410883E-5	0.0016414973874657136	0.0	0.08128538729645693	1.459108788858412E-4
52-53	3.126661690410883E-5	0.0016519195931004164	0.0	0.0901807398056759	1.7978304719862577E-4
54-55	3.387216831278457E-5	0.001685791761413201	0.0	0.09912038668884235	2.1365521551141033E-4
56-57	3.64777197214603E-5	0.0017196639297259856	0.0	0.10828411099315491	2.1365521551141033E-4
58-59	3.64777197214603E-5	0.0017274805839520128	0.0	0.11719509681082592	2.1365521551141033E-4
60-61	3.64777197214603E-5	0.0017509305466300944	0.0	0.1251967451868691	2.1365521551141033E-4
62-63	3.64777197214603E-5	0.0018004360233949334	0.0	0.13333909333898078	2.3449962678081623E-4
64-65	3.64777197214603E-5	0.0020531745100364797	0.0	0.14157003023898743	2.3710517818949196E-4
66-67	3.64777197214603E-5	0.0020948633325752917	0.0	0.1495821008206653	2.3971072959816768E-4
68-69	3.64777197214603E-5	0.0021365521551141033	0.0	0.15765409908474276	2.3971072959816768E-4
70-71	3.64777197214603E-5	0.0021573965663835093	0.0	0.16509294835651198	2.4492183241551916E-4
72-73	3.64777197214603E-5	0.0021626076692008605	0.0	0.17039785102457577	2.709773465022765E-4
74-75	3.64777197214603E-5	0.002191268734696294	0.0	0.1750096770179318	2.709773465022765E-4
76-77	3.64777197214603E-5	0.002277251931182593	0.0	0.17876167104642487	2.709773465022765E-4
78-79	3.64777197214603E-5	0.0022928852396346473	0.0	0.18206290468121705	2.709773465022765E-4
80-81	3.64777197214603E-5	0.002298096342451999	0.0	0.18374609089122157	2.7358289791095227E-4
82-83	3.64777197214603E-5	0.0023137296509040534	0.0	0.18443916756592932	2.76188449319628E-4
84-85	4.168882253881177E-5	0.0023293629593561078	0.0	0.18515308865190644	2.76188449319628E-4
86-87	4.168882253881177E-5	0.0023397851649908106	0.0	0.18547617702658226	2.76188449319628E-4
88-89	4.168882253881177E-5	0.0024361905671118128	0.0	0.18559863794279002	2.76188449319628E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	21110	0.0	46.47936	1
GTATCAA	36895	0.0	34.991875	1
TCAACGC	44435	0.0	28.369547	4
ATCAACG	44715	0.0	28.173199	3
CAACGCA	45650	0.0	27.532297	5
TATCAAC	47315	0.0	26.924944	2
AACGCAG	47350	0.0	26.658146	6
ACGCAGA	55045	0.0	22.901218	7
CGCAGAG	55760	0.0	22.600088	8
GTACATG	49290	0.0	21.224325	1
TACATGG	48825	0.0	20.744144	2
ACATGGG	49285	0.0	20.000805	3
GCAGAGT	67190	0.0	18.650068	9
CATGGGG	38215	0.0	18.318884	4
GAGTACT	41655	0.0	17.785984	12-13
AGAGTAC	60140	0.0	17.661547	10-11
CAGAGTA	65665	0.0	16.847782	10-11
GTGGTAT	11060	0.0	16.605673	1
AGTACTT	45490	0.0	15.627398	12-13
GTACTTT	47625	0.0	15.566212	14-15
>>END_MODULE