FastQCFastQC Report
Fri 27 May 2016
SRR2139882_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139882_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10831237
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117450.044.1167031
GTATCAA197350.033.5451051
CTTATAC102200.031.6217541
TCAACGC227650.028.2461784
ATCAACG228150.028.1481423
CAACGCA231500.027.7586215
AACGCAG239850.026.7578776
TATCAAC244600.026.5259592
TATACAC140100.026.0674363
ACGCAGA279150.023.0350787
CGCAGAG286500.022.4297398
TTATACA156400.021.7703842
TACATGG279100.021.2531722
GTACATG284250.021.1580471
ACATGGG283350.020.3224453
CATGGGG211300.018.8053024
ACACATC172750.018.539836
ACATCTC173750.018.40948
GCAGAGT349900.018.3183849
TACACAT196500.018.2079035
Produced by FastQC (version 0.11.5)