##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139882_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10831237
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.330202542885914	33.0	31.0	34.0	31.0	34.0
2	32.50632250037553	34.0	31.0	34.0	31.0	34.0
3	32.62807415256447	34.0	31.0	34.0	31.0	34.0
4	36.14498870258309	37.0	35.0	37.0	35.0	37.0
5	35.981229383125864	37.0	35.0	37.0	35.0	37.0
6	36.020685356621776	37.0	35.0	37.0	35.0	37.0
7	35.99794354052081	37.0	35.0	37.0	35.0	37.0
8	35.98160071652019	37.0	35.0	37.0	35.0	37.0
9	37.82324752011243	39.0	38.0	39.0	35.0	39.0
10-11	37.701102976511365	39.0	37.0	39.0	35.0	39.0
12-13	37.718459581301744	39.0	37.0	39.0	35.0	39.0
14-15	39.03458418461345	40.0	38.0	41.0	36.0	41.0
16-17	38.981878939589265	40.0	38.0	41.0	36.0	41.0
18-19	39.04559003740755	40.0	38.5	41.0	36.0	41.0
20-21	39.04595622333804	40.0	39.0	41.0	36.0	41.0
22-23	39.00628793492969	40.0	39.0	41.0	36.0	41.0
24-25	38.989208306756865	40.0	38.0	41.0	35.5	41.0
26-27	38.88904497798253	40.0	38.0	41.0	35.0	41.0
28-29	38.806456469895316	40.0	38.0	41.0	35.0	41.0
30-31	38.66149580020338	40.0	38.0	41.0	35.0	41.0
32-33	38.569740901484835	40.0	38.0	41.0	35.0	41.0
34-35	38.52299188234044	40.0	38.0	41.0	34.0	41.0
36-37	38.42281777401129	40.0	38.0	41.0	34.0	41.0
38-39	38.38116618583087	40.0	38.0	41.0	34.0	41.0
40-41	38.39716949351966	40.0	38.0	41.0	34.0	41.0
42-43	38.369325037183145	40.0	38.0	41.0	34.0	41.0
44-45	38.260875836104255	40.0	38.0	41.0	34.0	41.0
46-47	38.21071822425981	40.0	38.0	41.0	33.0	41.0
48-49	38.19432960412267	40.0	38.0	41.0	33.0	41.0
50-51	38.105357624144126	40.0	37.5	41.0	33.0	41.0
52-53	37.97798474445921	40.0	37.0	41.0	33.0	41.0
54-55	37.80725049880681	39.5	37.0	41.0	33.0	41.0
56-57	37.68078115724661	39.0	36.5	41.0	33.0	41.0
58-59	37.45701544700728	39.0	36.0	41.0	32.5	41.0
60-61	37.297514093896595	39.0	36.0	40.5	32.0	41.0
62-63	37.09590485173791	39.0	35.0	40.0	32.0	41.0
64-65	36.79527982949854	38.0	35.0	40.0	31.0	41.0
66-67	36.7351182730284	38.0	35.0	40.0	31.0	41.0
68-69	36.507663186773954	37.5	35.0	40.0	31.0	41.0
70-71	36.20311622573408	37.0	35.0	39.5	31.0	41.0
72-73	35.891978031014126	36.5	34.5	39.0	31.0	41.0
74-75	35.494148244288446	36.0	34.0	39.0	30.5	40.0
76-77	33.44899667009291	34.5	32.0	36.5	27.5	39.0
78-79	34.63739932456784	35.0	34.0	37.0	30.0	39.0
80-81	34.64509251754086	35.0	34.0	37.0	30.0	39.0
82-83	34.35965800970662	35.0	34.0	36.5	30.0	39.0
84-85	34.10048405819511	35.0	34.0	36.0	30.0	37.0
86-87	33.89019300308915	35.0	34.0	36.0	30.0	37.0
88-89	33.65107370017873	35.0	33.5	35.0	29.5	37.0
90-91	33.48600468676176	35.0	33.0	35.0	29.5	36.0
92-93	33.41947597524828	35.0	33.0	35.0	29.0	36.0
94-95	33.392669467430096	35.0	33.0	35.0	30.0	36.0
96-97	33.427345175600756	35.0	33.0	35.0	30.0	35.5
98-99	33.45430231509572	35.0	33.0	35.0	30.0	35.0
100-101	32.214631882002784	34.0	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	7.0
19	26.0
20	73.0
21	259.0
22	971.0
23	3241.0
24	8550.0
25	18499.0
26	34227.0
27	55712.0
28	83643.0
29	116234.0
30	155021.0
31	203557.0
32	266659.0
33	362279.0
34	523821.0
35	798367.0
36	1401447.0
37	2540328.0
38	2995196.0
39	1245471.0
40	17649.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.38548533283871	20.621162661291596	13.510211252879056	24.483140752990632
2	14.57443295074095	21.68555838320133	41.16055187627167	22.579456789786054
3	17.935056123363285	26.70307312309619	30.902190323923534	24.459680429616988
4	12.139697101918397	18.390185273441602	39.23618490746299	30.23393271717701
5	12.6819092652871	38.207583925879476	36.28849334110346	12.822013467729962
6	29.298989579860546	38.656886558755936	19.039856666417695	13.004267194965818
7	25.730754483536828	32.799540809604665	23.80189815807742	17.667806548781087
8	24.10336293487126	36.97791767001865	21.869103258606575	17.04961613650351
9	25.3563102442467	16.36687128810316	21.317670463930153	36.95914800371999
10-11	23.830869486419505	26.823677415119775	29.63986648611474	19.705586612345982
12-13	24.670437404861183	24.324303488644507	29.525951041953984	21.47930806454033
14-15	21.761195039919954	25.80799523673959	26.804594286164836	25.626215437175613
16-17	21.587965045000164	29.013774395650042	27.461164676102324	21.93709588324747
18-19	21.557916238006793	28.224472421755703	28.987155391392506	21.23045594884499
20-21	22.333382760358393	27.460016275833084	28.766510119029263	21.44009084477926
22-23	21.9678242877665	27.65530883554356	28.598677578273374	21.77818929841656
24-25	21.862197616513768	27.75314410479341	28.741926300017802	21.642731978675016
26-27	21.890275793729717	27.945568146743803	28.636860000995124	21.527296058531352
28-29	21.634254317301632	28.056055693569036	28.61473635073259	21.69495363839674
30-31	22.25113770464648	27.744197710935104	28.520144612307725	21.484519972110697
32-33	21.579059265550047	28.13953828798738	28.551935340335728	21.729467106126847
34-35	21.824847363786215	28.098319516685542	28.656502226417707	21.420330893110535
36-37	21.764577059077034	28.01961965791948	28.57479248312375	21.641010799879734
38-39	21.66302747301257	27.819359658135923	28.753729433821945	21.763883435029566
40-41	21.861096711646518	27.70152432987058	28.709642951895336	21.727736006587563
42-43	21.924888438921652	27.738617923539504	28.569183789504105	21.767309848034742
44-45	21.85481966854839	28.03201339387636	28.319365117772062	21.79380181980319
46-47	22.070787694960092	27.78461610661503	27.97307701332688	22.171519185098003
48-49	21.94702639018355	27.869180482345623	28.043604038672747	22.140189088798078
50-51	21.77962092897715	28.034298371810955	28.14253701109259	22.043543688119307
52-53	21.939159227307826	27.993575561066848	28.15316356714409	21.914101644481235
54-55	21.8856877901124	27.9649449534367	28.00212257371941	22.147244682731497
56-57	22.09950776752428	28.17842569902221	27.699303366568557	22.02276316688495
58-59	21.856736825688834	28.343831193976488	28.015538319047966	21.78389366128671
60-61	22.077021579933703	28.422824131765434	27.697685245415165	21.802469042885697
62-63	21.914655112060554	28.547880543199422	27.66248324328771	21.874981101452306
64-65	21.90761215433328	28.43266914664192	27.65335980416472	22.006358894860085
66-67	22.038055593936818	28.33363077644741	27.59056023564434	22.037753393971435
68-69	22.01668211580291	28.256583704464493	27.674931103209083	22.05180307652351
70-71	22.002107445194348	28.02188314952623	27.718270698574372	22.25773870670506
72-73	22.085482816352293	28.05265432723867	27.745633047196545	22.11622980921249
74-75	21.93045522801756	28.196592887429496	27.702182556117933	22.170769328435014
76-77	22.00927684551636	28.136089563229085	27.627852432194462	22.226781159060092
78-79	22.00987776090767	28.156019923099514	27.59368413698484	22.240418179007975
80-81	21.98503365907423	28.166075373122645	27.642766007665177	22.206124960137945
82-83	22.056696468565566	28.09346269286156	27.739772016064872	22.110068822508005
84-85	22.180150915283352	28.06969497201677	27.66344575488531	22.086708357814572
86-87	21.969806262062278	28.120204797472077	27.74892637044789	22.161062570017755
88-89	22.13654987674375	28.07246961118285	27.634155193155564	22.15682531891784
90-91	22.111253386369267	28.102046223218775	27.70008654192268	22.08661384848928
92-93	22.12446282652499	28.047746128260115	27.738860683140153	22.088930362074738
94-95	21.98822841563072	28.081880228856697	27.794846065000318	22.135045290512267
96-97	21.94279921304293	28.12568341320647	27.847168326443434	22.08434904730716
98-99	22.247142838510253	28.693474209420955	27.86591803292664	21.193464919142148
100-101	26.237445867022462	35.717652875295244	28.47996293134965	9.564938326332644
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1908.0
1	1783.5
2	2658.5
3	3933.5
4	5389.0
5	8912.0
6	11898.0
7	13623.5
8	14463.0
9	13913.5
10	12684.5
11	11512.5
12	12293.0
13	16024.0
14	24914.0
15	37168.5
16	47314.5
17	53660.5
18	56002.5
19	54829.5
20	52133.5
21	50265.5
22	51170.5
23	57095.0
24	68775.0
25	85761.0
26	108266.0
27	133503.0
28	159568.0
29	186712.5
30	211040.0
31	232813.5
32	258002.0
33	283017.0
34	306540.0
35	329971.0
36	355240.5
37	377474.0
38	394230.5
39	408144.0
40	422105.0
41	435781.5
42	444208.5
43	451407.0
44	458469.5
45	466679.0
46	477224.0
47	487533.5
48	485640.5
49	474592.0
50	463527.0
51	445557.0
52	418742.0
53	390120.0
54	363595.5
55	335684.5
56	306088.5
57	275009.0
58	242901.0
59	211043.0
60	177657.0
61	144734.0
62	115083.5
63	89710.5
64	68456.5
65	51544.5
66	38985.5
67	29937.5
68	23093.0
69	16880.5
70	12118.5
71	8661.0
72	6069.0
73	4207.5
74	2881.5
75	2030.0
76	1439.0
77	1008.0
78	687.0
79	459.5
80	299.5
81	196.0
82	142.5
83	102.0
84	78.0
85	55.0
86	39.5
87	33.5
88	22.0
89	10.0
90	6.5
91	6.0
92	4.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004394696561436149
3	7.38604464106916E-5
4	2.769766740400935E-5
5	1.2002322541737384E-4
6	0.0
7	0.0
8	3.969998994574673E-4
9	1.477208928213832E-4
10-11	3.69302232053458E-5
12-13	2.26197617132743E-4
14-15	4.616277900668225E-6
16-17	4.985580132721683E-4
18-19	0.0
20-21	2.3082353822107714E-5
22-23	0.0
24-25	0.0
26-27	9.701748837245402E-5
28-29	4.623653129843276E-6
30-31	4.168941634956075E-5
32-33	3.996484766352721E-4
34-35	0.0
36-37	3.332386966724802E-4
38-39	0.001140859401339473
40-41	0.0
42-43	0.0
44-45	3.219671451065921E-4
46-47	0.0
48-49	0.0
50-51	4.047815836522886E-5
52-53	5.421697038229204E-4
54-55	0.0033251377049154894
56-57	0.0035810770235742196
58-59	0.004243523057712975
60-61	0.0010482893127979728
62-63	5.448229668596175E-6
64-65	5.519939373401874E-4
66-67	0.0
68-69	0.0
70-71	5.75176057077251E-6
72-73	2.6802309659901995E-4
74-75	5.89988427376997E-6
76-77	0.0
78-79	0.0
80-81	5.831565959614871E-4
82-83	0.0
84-85	0.0
86-87	3.8385596495855665E-5
88-89	0.0
90-91	0.0012537368782413634
92-93	2.080665104064801E-4
94-95	6.83186900191791E-6
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	305.0
20-21	834.0
22-23	1814.0
24-25	4142.0
26-27	7048.0
28-29	15234.0
30-31	33079.0
32-33	40830.0
34-35	55054.0
36-37	85138.0
38-39	100399.0
40-41	97819.0
42-43	109363.0
44-45	126180.0
46-47	126154.0
48-49	118395.0
50-51	108171.0
52-53	104440.0
54-55	115551.0
56-57	123852.0
58-59	124305.0
60-61	126371.0
62-63	119523.0
64-65	120200.0
66-67	128218.0
68-69	117087.0
70-71	113520.0
72-73	107247.0
74-75	108066.0
76-77	106138.0
78-79	112717.0
80-81	115236.0
82-83	108647.0
84-85	107209.0
86-87	111995.0
88-89	119811.0
90-91	130522.0
92-93	127448.0
94-95	143673.0
96-97	218091.0
98-99	866806.0
100-101	6124605.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.63031364200786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.91047779826545	74.35611144329715
2	7.453340227856504	12.466503618724674
3	1.9943351331826706	5.003606180860267
4	0.7549545987863953	2.5254835952792978
5	0.3489407942092486	1.4591014031105392
6	0.1876847056800861	0.941767848110012
7	0.1020384128460139	0.597345312889138
8	0.06411191107927806	0.4289359385398834
9	0.04731941499291307	0.35616037654922844
>10	0.13420269160997483	1.6588388467237103
>50	0.0020902134142398664	0.11798782166254028
>100	4.6695329175925715E-4	0.05891066502447999
>500	1.857259798588843E-5	0.010561978924006054
>1k	1.857218748145733E-5	0.018684970305032158
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.232555801336449E-6
2	0.0	2.769766740400935E-5	0.0	0.0	9.232555801336449E-6
3	0.0	3.6930223205345795E-5	0.0	0.0	9.232555801336449E-6
4	0.0	9.23255580133645E-5	0.0	0.0	2.769766740400935E-5
5	0.0	1.0155811381470094E-4	0.0	0.0	2.769766740400935E-5
6	0.0	1.107906696160374E-4	0.0	0.0	2.769766740400935E-5
7	9.232555801336449E-6	1.107906696160374E-4	0.0	0.0	2.769766740400935E-5
8	9.232555801336449E-6	1.107906696160374E-4	0.0	0.0	2.769766740400935E-5
9	9.232555801336449E-6	2.0311622762940188E-4	0.0	0.0	3.6930223205345795E-5
10-11	1.8465111602672897E-5	2.0311622762940188E-4	0.0	9.232555801336449E-6	3.6930223205345795E-5
12-13	2.769766740400935E-5	2.077325055300701E-4	0.0	9.232555801336449E-6	3.6930223205345795E-5
14-15	2.769766740400935E-5	2.9544178564276636E-4	0.0	1.8465111602672897E-5	6.462789060935515E-5
16-17	2.769766740400935E-5	3.1390689724543927E-4	0.0	1.8465111602672897E-5	9.23255580133645E-5
18-19	2.769766740400935E-5	3.4622084255011686E-4	0.0	1.8465111602672897E-5	1.292557812187103E-4
20-21	2.769766740400935E-5	3.785347878547944E-4	0.0	4.154650110601402E-5	1.3387205911937852E-4
22-23	2.769766740400935E-5	4.10848733159472E-4	0.0	6.462789060935515E-5	1.523371707220514E-4
24-25	2.769766740400935E-5	4.385464005634813E-4	0.0	1.0617439171536917E-4	1.661860044240561E-4
26-27	2.769766740400935E-5	4.662440679674907E-4	0.0	2.0311622762940188E-4	1.661860044240561E-4
28-29	2.769766740400935E-5	4.708603458681589E-4	0.0	8.263137442196122E-4	1.661860044240561E-4
30-31	2.769766740400935E-5	5.493370701795188E-4	0.0	0.003083673637646374	1.661860044240561E-4
32-33	3.2313945304677574E-5	5.954998491862009E-4	0.0	0.006887486627796991	1.84651116026729E-4
34-35	3.6930223205345795E-5	6.878254071995655E-4	0.0	0.012223903880969458	1.9388367182806543E-4
36-37	3.6930223205345795E-5	8.078486326169393E-4	0.0	0.019905390307681386	1.9388367182806543E-4
38-39	3.6930223205345795E-5	8.863253569282992E-4	0.0	0.03302485210138048	2.0311622762940188E-4
40-41	9.23255580133645E-5	9.924997486436683E-4	0.0	0.04541032570887332	2.1234878343073834E-4
42-43	9.23255580133645E-5	0.0010155811381470094	0.0	0.055538439422939415	2.1696506133140657E-4
44-45	1.1079066961603738E-4	0.0010940578624583693	0.0	0.0668714016690799	2.215813392320748E-4
46-47	1.292557812187103E-4	0.0011679183088690607	0.0	0.07984775884785827	2.4004645083474768E-4
48-49	1.3848833702004675E-4	0.0011817671425710654	0.0	0.09204396506142373	2.585115624374206E-4
50-51	1.4772089282138318E-4	0.0013525694248957899	0.0	0.10390779926614108	2.585115624374206E-4
52-53	1.4772089282138318E-4	0.001357185702796458	0.0	0.11473297094320806	2.723603961394253E-4
54-55	1.4772089282138318E-4	0.0013618019806971263	0.0	0.1257243286246991	2.9082550774209813E-4
56-57	1.4772089282138318E-4	0.0013848833702004675	0.0	0.13469375658569746	2.9544178564276636E-4
58-59	1.4772089282138318E-4	0.0014033484818031404	0.0	0.14249526623782677	3.000580635434346E-4
60-61	1.523371707220514E-4	0.0014910577619158365	0.0	0.14962741559435916	3.046743414441028E-4
62-63	1.5695344862271963E-4	0.0015326042630218505	0.0	0.15611328604479802	3.231394530467757E-4
64-65	1.661860044240561E-4	0.0017172553790485796	0.0	0.16276995877756162	3.231394530467757E-4
66-67	1.8003483812606077E-4	0.001763418158055262	0.0	0.16932507339651048	3.231394530467757E-4
68-69	1.9388367182806543E-4	0.001809580937061944	0.0	0.17598636240717475	3.231394530467757E-4
70-71	1.9388367182806543E-4	0.0018372786044659533	0.0	0.18262457002833565	3.323720088481122E-4
72-73	1.9388367182806543E-4	0.001851127438167958	0.0	0.18805792911742214	3.969998994574673E-4
74-75	1.9388367182806543E-4	0.0019203716066779814	0.0	0.19253110240316965	3.969998994574673E-4
76-77	1.9388367182806543E-4	0.002026545998393351	0.0	0.196616508345261	3.969998994574673E-4
78-79	1.9849994972873366E-4	0.0020865576111020376	0.0	0.20012487954976887	3.969998994574673E-4
80-81	2.0311622762940188E-4	0.0021004064448040425	0.0	0.2015605419768767	4.154650110601402E-4
82-83	2.0311622762940188E-4	0.00213272039010872	0.0	0.20209141393545355	4.5239523426548605E-4
84-85	2.0311622762940188E-4	0.0021604180575127293	0.0	0.20262690217193105	4.570115121661542E-4
86-87	2.077325055300701E-4	0.002178883169115402	0.0	0.20283925095536182	4.7547662376882713E-4
88-89	2.1696506133140657E-4	0.002234278503923421	0.0	0.20289464629016982	4.8009290166949536E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11745	0.0	44.116703	1
GTATCAA	19735	0.0	33.545105	1
CTTATAC	10220	0.0	31.621754	1
TCAACGC	22765	0.0	28.246178	4
ATCAACG	22815	0.0	28.148142	3
CAACGCA	23150	0.0	27.758621	5
AACGCAG	23985	0.0	26.757877	6
TATCAAC	24460	0.0	26.525959	2
TATACAC	14010	0.0	26.067436	3
ACGCAGA	27915	0.0	23.035078	7
CGCAGAG	28650	0.0	22.429739	8
TTATACA	15640	0.0	21.770384	2
TACATGG	27910	0.0	21.253172	2
GTACATG	28425	0.0	21.158047	1
ACATGGG	28335	0.0	20.322445	3
CATGGGG	21130	0.0	18.805302	4
ACACATC	17275	0.0	18.53983	6
ACATCTC	17375	0.0	18.4094	8
GCAGAGT	34990	0.0	18.318384	9
TACACAT	19650	0.0	18.207903	5
>>END_MODULE