FastQCFastQC Report
Fri 27 May 2016
SRR2139881_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139881_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12092598
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA220700.036.4494441
CTTATAC249950.033.8018461
TATACAC274600.031.9542223
GTATCAA346500.029.3912491
TTATACA297350.029.0209122
TACACAT366350.024.2944245
ATCAACG415050.024.0167143
CAACGCA420250.023.682175
ACACATC364650.023.5463646
AACGCAG427450.023.522076
TATCAAC428950.023.458122
TCAACGC434000.022.9861494
ATACACA399650.022.830094
ACATCTC380250.022.4771128
CACATCT395100.021.7416047
TACATGG455150.021.6765022
GTACATG471950.021.320821
CGCAGAG470450.021.1885158
ACGCAGA472250.021.1410067
ACATGGG464100.020.717083
Produced by FastQC (version 0.11.5)