##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139881_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12092598
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.145963754025395	33.0	31.0	34.0	30.0	34.0
2	32.36731866882534	34.0	31.0	34.0	30.0	34.0
3	32.413391233215556	34.0	31.0	34.0	30.0	34.0
4	36.0584603903975	37.0	35.0	37.0	35.0	37.0
5	35.91713385328777	37.0	35.0	37.0	35.0	37.0
6	35.940311089478044	37.0	35.0	37.0	35.0	37.0
7	35.88853247250922	37.0	35.0	37.0	35.0	37.0
8	35.890703056530946	37.0	35.0	37.0	35.0	37.0
9	37.6718724958855	39.0	37.0	39.0	35.0	39.0
10-11	37.55219846057894	39.0	37.0	39.0	35.0	39.0
12-13	37.51521947558333	39.0	37.0	39.0	35.0	39.0
14-15	38.77301722094789	40.0	38.0	41.0	34.5	41.0
16-17	38.74888832821533	40.0	38.0	41.0	34.0	41.0
18-19	38.74962787152934	40.0	38.0	41.0	34.0	41.0
20-21	38.75944761154757	40.0	38.0	41.0	34.0	41.0
22-23	38.67342847407106	40.0	38.0	41.0	34.0	41.0
24-25	38.60438731047843	40.0	38.0	41.0	34.0	41.0
26-27	38.4298192122339	40.0	38.0	41.0	34.0	41.0
28-29	38.30024338770109	40.0	38.0	41.0	34.0	41.0
30-31	38.168136908830874	40.0	38.0	41.0	33.5	41.0
32-33	38.05649086507451	40.0	37.0	41.0	33.0	41.0
34-35	38.08614957330329	40.0	37.0	41.0	33.0	41.0
36-37	38.05083238969402	40.0	37.0	41.0	33.0	41.0
38-39	37.95059002924481	40.0	37.0	41.0	33.0	41.0
40-41	37.93067541221811	40.0	37.0	41.0	33.0	41.0
42-43	37.93035844162901	40.0	37.0	41.0	33.0	41.0
44-45	37.98368473014146	40.0	37.0	41.0	33.0	41.0
46-47	37.98451881994583	40.0	37.0	41.0	33.0	41.0
48-49	37.91556418899117	40.0	37.0	41.0	33.0	41.0
50-51	37.79273145095232	40.0	37.0	41.0	33.0	41.0
52-53	37.57382799770501	39.5	36.0	41.0	32.0	41.0
54-55	37.46788087832392	39.0	36.0	41.0	32.0	41.0
56-57	37.35934461191521	39.0	36.0	41.0	32.0	41.0
58-59	37.19546554835164	39.0	35.0	41.0	31.5	41.0
60-61	37.00369656894235	39.0	35.0	40.5	31.0	41.0
62-63	36.75806370125217	38.0	35.0	40.0	31.0	41.0
64-65	36.48623405473761	38.0	35.0	40.0	31.0	41.0
66-67	36.22048307889074	37.0	35.0	40.0	31.0	41.0
68-69	35.95788059723773	37.0	34.5	40.0	30.0	41.0
70-71	35.67286479257436	36.5	34.0	39.0	30.0	41.0
72-73	35.36352985271736	36.0	34.0	39.0	30.0	40.5
74-75	35.01261892192535	35.5	34.0	38.5	30.0	40.0
76-77	33.649258414430115	34.5	32.0	36.5	28.0	39.0
78-79	34.35904556377257	35.0	33.5	37.0	29.0	39.0
80-81	34.305036748315736	35.0	34.0	36.5	30.0	39.0
82-83	34.024736353184565	35.0	34.0	36.0	29.0	37.5
84-85	33.85292935530177	35.0	34.0	36.0	29.0	37.0
86-87	33.63215121927277	35.0	33.0	35.5	29.0	37.0
88-89	33.53693502558162	35.0	33.0	35.0	29.0	36.0
90-91	33.36403400447888	35.0	33.0	35.0	29.0	36.0
92-93	33.26724999856363	35.0	33.0	35.0	29.0	36.0
94-95	33.269661732839594	35.0	33.0	35.0	29.0	36.0
96-97	33.30185263855216	35.0	33.0	35.0	29.0	35.0
98-99	33.39204322114111	35.0	33.0	35.0	30.0	35.0
100	32.16534532168319	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	14.0
20	57.0
21	363.0
22	1714.0
23	6071.0
24	16198.0
25	33763.0
26	60771.0
27	96511.0
28	138601.0
29	185496.0
30	237003.0
31	296820.0
32	369693.0
33	472426.0
34	634126.0
35	875104.0
36	1357950.0
37	2352165.0
38	3015538.0
39	1859767.0
40	82446.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.74999433537773	20.901612705557564	13.56836636759115	24.78002659147356
2	14.557326721685449	21.295200584688253	41.67135962015772	22.47611307346858
3	17.412370774253805	27.427795085886427	31.489957741090873	23.6698763987689
4	11.853011238775984	18.83214839358755	38.84367114494338	30.47116922269309
5	12.315608275409469	38.58262715753885	36.26825269474765	12.833511872304031
6	28.56439120857239	38.724366757251005	19.142114870601006	13.569127163575603
7	25.17717036487941	33.18906326002072	23.735900258984877	17.897866116115
8	24.441439300305856	36.246867711967276	21.918036140786292	17.393656846940583
9	25.7065024405839	15.82170349167317	21.269565067820828	37.2022289999221
10-11	23.884342305929625	26.596464217201298	29.383408759639572	20.135784717229498
12-13	24.924251182417542	24.365475475162572	28.89168233327528	21.8185910091446
14-15	22.11013712686058	25.551163612649656	26.571692865337955	25.76700639515181
16-17	21.612512405988316	28.58240059197285	27.423334062300793	22.381752939738043
18-19	21.44011834686028	27.70964812788857	29.007331308153645	21.842902217097503
20-21	22.401618845133058	26.985951214171067	28.550769235095014	22.061660705600865
22-23	22.093562060222784	27.713194036151933	28.263607265854972	21.92963663777031
24-25	21.98923291990125	27.814177831682542	28.64978297101301	21.546806277403203
26-27	21.972830729701272	28.06828077506341	28.367683286205274	21.591205209030047
28-29	21.50805983521732	27.86149063193313	28.47610603063066	22.15434350221889
30-31	22.416530551486076	27.82204669078633	28.422680350746283	21.338742406981318
32-33	22.12707334058409	27.959993732155063	28.511608296561324	21.40132463069952
34-35	22.012102725213857	28.104836897106605	28.7510425260822	21.132017851597336
36-37	22.08282612003105	27.79507883080515	28.45811020319953	21.66398484596426
38-39	21.517635223267977	27.523583180376836	28.89935056499343	22.059431031361758
40-41	21.652528873451153	27.367681967797303	29.071697238096984	21.90809192065456
42-43	22.00099866556028	27.36786148685333	28.752972739453536	21.878167108132857
44-45	21.48554311851924	28.17064512056739	28.455742945815704	21.888068815097668
46-47	22.17622046581077	27.409432539246144	27.77541712352359	22.638929871419503
48-49	22.073284934452467	27.463142839372217	27.90282515123171	22.560747074943606
50-51	21.807219434350884	27.709439000015962	27.956173922911127	22.527167642722027
52-53	21.6913979135347	27.9524400607107	28.286014905926127	22.07014711982848
54-55	21.718219253285493	27.9759841092661	27.738690302787322	22.567106334661087
56-57	22.391142055229565	28.23558955999076	27.071235552569416	22.302032832210262
58-59	21.644468761848653	28.528782814968544	28.069644542627454	21.757103880555345
60-61	22.172265939445712	29.077006481550516	26.993007912066247	21.757719666937522
62-63	21.730856411816855	29.159222492653274	27.081314879605113	22.02860621592476
64-65	21.629435316293204	28.957927002523064	27.18973116137629	22.222906519807438
66-67	21.880516132454776	28.77883683242765	27.030626594143563	22.310020440974014
68-69	22.05059116414139	28.34001707430226	27.19221845061898	22.41717331093737
70-71	22.152200707946488	27.790642958691258	27.407835690559306	22.64932064280295
72-73	22.16633020042801	27.77052476574185	27.579599474254184	22.48354555957596
74-75	22.2857575564497	27.90041781949621	27.357366807000812	22.456457817053284
76-77	22.199980071375297	27.86517905962692	27.33015327587422	22.60468759312356
78-79	22.13564387918084	27.83595934745224	27.413888936035512	22.614507837331413
80-81	22.180167098300938	27.823375244106046	27.405330521685872	22.591127135907147
82-83	22.477211825260866	27.637519968226698	27.610353525928115	22.274914680584313
84-85	22.579060788975397	27.75269589647187	27.2656569005084	22.40258641404433
86-87	22.255898424507752	27.8896585487809	27.445603911949924	22.408839114761427
88-89	22.524566318434978	27.578343371037363	27.455342268718574	22.441748041809085
90-91	22.534143706183563	27.51058300170412	27.564950799958705	22.39032249215361
92-93	22.593215077130594	27.52072022068324	27.58050644023065	22.305558261955515
94-95	22.067130458972258	27.649531652318736	27.798123888542705	22.485214000166298
96-97	22.262087766872703	27.79482008347725	27.787830019384728	22.155262130265317
98-99	23.276917358873003	29.81697316260874	28.029390922717234	18.87671855580102
100	29.927092159540038	40.99427836933976	29.078629471120205	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2115.0
1	2245.0
2	4264.0
3	6495.5
4	9671.5
5	17485.5
6	23461.0
7	25861.0
8	25689.0
9	23022.0
10	19937.0
11	17246.5
12	18992.0
13	26882.0
14	43113.0
15	64904.0
16	81484.5
17	88837.0
18	88933.5
19	84221.5
20	76740.5
21	69508.0
22	66544.5
23	69315.0
24	79599.5
25	96680.5
26	119919.0
27	143323.5
28	165688.5
29	194588.0
30	223082.5
31	248793.0
32	277484.0
33	308001.0
34	338947.0
35	374404.5
36	408583.5
37	438430.5
38	457923.5
39	462184.5
40	467396.0
41	475065.0
42	483909.5
43	498924.5
44	521046.5
45	542819.5
46	569074.5
47	601523.5
48	633531.0
49	625968.0
50	589479.5
51	569075.0
52	541984.0
53	499141.5
54	450890.0
55	406750.5
56	364833.0
57	318879.0
58	275047.5
59	231224.5
60	188325.0
61	150594.5
62	117053.5
63	89482.5
64	66636.0
65	50022.0
66	37048.0
67	27952.0
68	21268.0
69	15648.0
70	11258.0
71	7646.0
72	5370.5
73	3468.5
74	2265.5
75	1553.0
76	1035.0
77	671.5
78	454.5
79	320.5
80	237.5
81	176.5
82	125.5
83	86.5
84	62.5
85	42.5
86	31.5
87	27.0
88	18.5
89	9.5
90	5.5
91	3.5
92	2.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0012528325178758112
18-19	7.029093334616763E-5
20-21	0.0
22-23	0.007162642436092439
24-25	0.008020537208053893
26-27	0.020350874231017925
28-29	0.016423714949903943
30-31	0.0020506105630686035
32-33	0.006247434871221307
34-35	0.03352838903370352
36-37	0.0057579109402211654
38-39	0.002816423343526817
40-41	0.0076269362402005365
42-43	0.00743912266687055
44-45	0.008790141441407393
46-47	0.005480167961704151
48-49	0.0011803041357903025
50-51	0.0027806938392885357
52-53	9.915299150031144E-4
54-55	0.005850039875929162
56-57	0.0010735935704628253
58-59	0.0014792758418568745
60-61	9.199698512737311E-4
62-63	0.0016881639940148408
64-65	4.4027803977524224E-4
66-67	0.006160148459577876
68-69	0.004569170610374048
70-71	0.0018855227394876683
72-73	0.001804030486871069
74-75	8.962674425278557E-4
76-77	8.288931608667398E-4
78-79	8.723697532671286E-4
80-81	0.001183786688403245
82-83	0.008085328933930627
84-85	8.695708749059111E-4
86-87	7.64161585788123E-5
88-89	5.19071430522581E-4
90-91	3.909430294625931E-4
92-93	0.0029607665905742875
94-95	0.003928249891105582
96-97	8.58553706183175E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	283.0
20-21	1270.0
22-23	2533.0
24-25	4064.0
26-27	6312.0
28-29	20325.0
30-31	53024.0
32-33	68121.0
34-35	88526.0
36-37	129318.0
38-39	150723.0
40-41	148016.0
42-43	169186.0
44-45	182785.0
46-47	180692.0
48-49	167565.0
50-51	146866.0
52-53	134908.0
54-55	150257.0
56-57	170287.0
58-59	178273.0
60-61	183431.0
62-63	171618.0
64-65	181869.0
66-67	197649.0
68-69	175455.0
70-71	152903.0
72-73	139443.0
74-75	137329.0
76-77	138028.0
78-79	146316.0
80-81	147794.0
82-83	140444.0
84-85	139495.0
86-87	143242.0
88-89	156624.0
90-91	165660.0
92-93	172365.0
94-95	192411.0
96-97	402930.0
98-99	2207274.0
100-101	4446984.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.99907510749112
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.86637435086968	71.98094130576516
2	6.871018242716476	11.13092245413467
3	1.972575733355097	4.793304299437317
4	0.8899986798924713	2.883562796727129
5	0.47360619314191904	1.9180831804837624
6	0.2789935337214554	1.3558930915445113
7	0.17625692054164122	0.9993653281617243
8	0.11913328558905234	0.7719748757783872
9	0.08460955527462156	0.6167966150250445
>10	0.2629557898779218	3.1365132487507927
>50	0.0031782164420395623	0.17572352566751567
>100	0.0012209609767828272	0.16177449168607955
>500	3.9268950774825783E-5	0.023489823661353253
>1k	3.926865003470906E-5	0.051654963176539885
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.269521570137369E-6	0.0	0.0	0.0	0.0
2	4.961712942082421E-5	0.0	0.0	0.0	0.0
3	4.961712942082421E-5	0.0	0.0	5.788665099096158E-5	0.0
4	4.961712942082421E-5	0.0	0.0	5.788665099096158E-5	0.0
5	1.323123451221979E-4	0.0	0.0	1.1577330198192316E-4	0.0
6	1.5712090983261E-4	0.0	0.0	1.1577330198192316E-4	0.0
7	2.315466039638463E-4	0.0	0.0	2.4808564710412104E-4	0.0
8	2.563551686742584E-4	0.0	0.0	2.646246902443958E-4	0.0
9	2.563551686742584E-4	0.0	0.0	2.9770277652494526E-4	0.0
10-11	2.894332549548079E-4	0.0	0.0	3.0597229809508264E-4	0.0
12-13	3.679937098711129E-4	0.0	0.0	3.390503843756321E-4	0.0
14-15	4.8790177263810476E-4	0.0	0.0	4.0934131772179974E-4	0.0
16-17	5.209798589186543E-4	0.0	0.0	4.7136272949783E-4	0.0
18-19	6.202141177603026E-4	0.0	0.0	5.457884236290662E-4	0.0
20-21	6.863702903214015E-4	0.0	0.0	7.607959844526379E-4	0.0
22-23	7.277178981720884E-4	0.0	0.0	0.0014637053179143143	0.0
24-25	0.001008881631556759	0.0	0.0	0.003506277145738244	0.0
26-27	0.001021285913911965	0.0	0.0	0.008898005209467808	0.0
28-29	0.001347932015932391	0.0	0.0	0.03028712275062811	0.0
30-31	0.0014719748394844516	0.0	0.0	0.03359906613946813	0.0
32-33	0.0014885138826247262	0.0	0.0	0.0407067199290012	0.0
34-35	0.0016125567061767868	0.0	0.0	0.04955924276983325	0.0
36-37	0.001819294745430221	0.0	0.0	0.06326597477233593	0.0
38-39	0.001988819937618037	0.0	0.0	0.10324084204237997	0.0
40-41	0.002005358980758312	0.0	0.0	0.14639534035614182	0.0
42-43	0.002133536565095441	0.0	0.0	0.1709434151370946	0.0
44-45	0.0023898917337696994	0.0	0.0	0.20056484140132666	0.0
46-47	0.002654516424014095	0.0	0.0	0.23111658884219916	0.0
48-49	0.0027950982907064305	0.0	0.0	0.2590510327061232	0.0
50-51	0.0027950982907064305	0.0	0.0	0.2865802700131105	0.0
52-53	0.002803367812276568	0.0	0.0	0.3588104061674754	0.0
54-55	0.002852984941697392	0.0	0.0	0.44276672390829497	0.0
56-57	0.0030101058515300022	0.0	0.0	0.52403544713882	0.0
58-59	0.003051453459380689	8.269521570137369E-6	0.0	0.5743306773283954	0.0
60-61	0.0030969358280164444	8.269521570137369E-6	0.0	0.607776757318816	0.0
62-63	0.0031465529574372687	8.269521570137369E-6	0.0	0.6331807275822781	0.0
64-65	0.0031920353260730242	8.269521570137369E-6	0.0	0.6433067567449112	0.0
66-67	0.0033574257574757716	8.269521570137369E-6	0.0	0.6521923576720239	0.0
68-69	0.003390503843756321	8.269521570137369E-6	0.0	0.6611606538148378	0.0
70-71	0.0034483904947472826	8.269521570137369E-6	0.0	0.6693433454084887	0.0
72-73	0.0035269509496635874	8.269521570137369E-6	0.0	0.6758349198410465	0.0
74-75	0.003576568079084412	8.269521570137369E-6	0.0	0.681160491732215	0.0
76-77	0.003588972361439618	8.269521570137369E-6	0.0	0.6859278709173993	0.0
78-79	0.0036179156869350984	8.269521570137369E-6	0.0	0.6890248067454157	0.0
80-81	0.003948696549740593	8.269521570137369E-6	0.0	0.6900254188554023	0.0
82-83	0.004002448439946486	8.269521570137369E-6	0.0	0.6906539024947327	0.0
84-85	0.004072739373292654	8.269521570137369E-6	0.0	0.6909598747928278	0.0
86-87	0.004134760785068685	8.269521570137369E-6	0.0	0.6911211304634455	0.0
88	0.004143030306638822	8.269521570137369E-6	0.0	0.6911252652242306	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	22070	0.0	36.449444	1
CTTATAC	24995	0.0	33.801846	1
TATACAC	27460	0.0	31.954222	3
GTATCAA	34650	0.0	29.391249	1
TTATACA	29735	0.0	29.020912	2
TACACAT	36635	0.0	24.294424	5
ATCAACG	41505	0.0	24.016714	3
CAACGCA	42025	0.0	23.68217	5
ACACATC	36465	0.0	23.546364	6
AACGCAG	42745	0.0	23.52207	6
TATCAAC	42895	0.0	23.45812	2
TCAACGC	43400	0.0	22.986149	4
ATACACA	39965	0.0	22.83009	4
ACATCTC	38025	0.0	22.477112	8
CACATCT	39510	0.0	21.741604	7
TACATGG	45515	0.0	21.676502	2
GTACATG	47195	0.0	21.32082	1
CGCAGAG	47045	0.0	21.188515	8
ACGCAGA	47225	0.0	21.141006	7
ACATGGG	46410	0.0	20.71708	3
>>END_MODULE