FastQCFastQC Report
Fri 27 May 2016
SRR2139880_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139880_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9552893
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA176150.037.7114031
GTATCAA288100.030.7201861
TGCGCGA9600.028.70998494
CTTATAC128950.026.1451971
ATCAACG342200.025.2424893
AACGCAG346500.025.0782226
CAACGCA347650.024.8342275
TCAACGC349850.024.746644
TATCAAC355350.024.7038082
TATACAC150150.024.5030943
CGCAGAG388400.022.2698638
ACGCAGA391150.022.103077
TACATGG351450.021.8485892
GTACATG367750.021.4891851
ACATGGG355550.021.212873
CGACGGG7301.8189894E-1220.9753394
TTATACA171150.020.6567062
ACACATC179200.019.034936
GCAGAGT455550.018.8555189
CATGGGG218900.018.52394
Produced by FastQC (version 0.11.5)