##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139880_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9552893
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00204451154221	31.0	31.0	34.0	30.0	34.0
2	32.106546467127814	33.0	31.0	34.0	30.0	34.0
3	31.83265048608835	31.0	31.0	34.0	30.0	34.0
4	35.90037091381637	37.0	35.0	37.0	35.0	37.0
5	35.84593305923138	37.0	35.0	37.0	35.0	37.0
6	35.88934273627895	37.0	35.0	37.0	35.0	37.0
7	35.870825099789144	37.0	35.0	37.0	35.0	37.0
8	35.87201636195444	37.0	35.0	37.0	35.0	37.0
9	37.64842702624221	39.0	37.0	39.0	35.0	39.0
10-11	37.553972707534776	39.0	37.0	39.0	35.0	39.0
12-13	37.50739273432666	39.0	37.0	39.0	35.0	39.0
14-15	38.74055571437888	40.0	38.0	41.0	34.0	41.0
16-17	38.67794065106769	40.0	38.0	41.0	34.0	41.0
18-19	38.709450791503684	40.0	38.0	41.0	34.0	41.0
20-21	38.79046657613665	40.0	38.0	41.0	34.0	41.0
22-23	38.74471602635228	40.0	38.0	41.0	34.0	41.0
24-25	38.64681238878056	40.0	38.0	41.0	34.0	41.0
26-27	38.49600313034351	40.0	38.0	41.0	34.0	41.0
28-29	38.37409239180373	40.0	38.0	41.0	34.0	41.0
30-31	38.236567585933415	40.0	38.0	41.0	34.0	41.0
32-33	38.03253964153523	40.0	37.0	41.0	33.0	41.0
34-35	38.10868778317063	40.0	37.0	41.0	33.0	41.0
36-37	38.075304470934896	40.0	37.0	41.0	33.0	41.0
38-39	38.02701434302025	40.0	37.0	41.0	33.0	41.0
40-41	37.984802155048264	40.0	37.0	41.0	33.0	41.0
42-43	38.019132682525935	40.0	37.0	41.0	33.0	41.0
44-45	38.05604678698627	40.0	37.0	41.0	33.0	41.0
46-47	38.01667956875118	40.0	37.0	41.0	33.0	41.0
48-49	37.940212610314816	40.0	37.0	41.0	33.0	41.0
50-51	37.84904498998532	40.0	37.0	41.0	33.0	41.0
52-53	37.59795767642218	40.0	36.5	41.0	32.0	41.0
54-55	37.554983860169955	39.5	36.0	41.0	32.0	41.0
56-57	37.38754469885951	39.0	36.0	41.0	31.5	41.0
58-59	37.223311536756626	39.0	35.5	41.0	31.0	41.0
60-61	37.07518526433876	39.0	35.0	41.0	31.0	41.0
62-63	36.84224929523083	38.5	35.0	40.0	31.0	41.0
64-65	36.50175921564227	38.0	35.0	40.0	31.0	41.0
66-67	36.26858820390652	37.5	35.0	40.0	31.0	41.0
68-69	35.98818650522066	37.0	34.5	40.0	30.0	41.0
70-71	35.72384734408318	36.5	34.0	39.0	30.0	41.0
72-73	35.43019839605415	36.0	34.0	39.0	30.0	40.5
74-75	35.09992443953813	36.0	34.0	38.5	30.0	40.0
76-77	33.83013287748688	34.5	32.5	37.0	28.5	39.0
78-79	34.44217443181351	35.0	34.0	37.0	29.0	39.0
80-81	34.36345487409409	35.0	34.0	37.0	30.0	39.0
82-83	34.07836728676861	35.0	34.0	36.0	29.0	38.0
84-85	33.83780812065561	35.0	34.0	36.0	29.0	37.0
86-87	33.63561723932547	35.0	33.0	35.5	29.0	37.0
88-89	33.46926044138034	35.0	33.0	35.0	29.0	36.5
90-91	33.336464578562186	35.0	33.0	35.0	29.0	36.0
92-93	33.30922477637859	35.0	33.0	35.0	29.0	36.0
94-95	33.30182443088591	35.0	33.0	35.0	29.0	36.0
96-97	33.288866693345554	35.0	33.0	35.0	29.0	35.0
98-99	33.39221112334563	35.0	33.0	35.0	29.5	35.0
100	32.29741007151924	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	0.0
18	1.0
19	7.0
20	46.0
21	291.0
22	1336.0
23	4825.0
24	12728.0
25	26490.0
26	48340.0
27	76449.0
28	109781.0
29	147918.0
30	187855.0
31	235516.0
32	295520.0
33	376489.0
34	506314.0
35	694785.0
36	1077023.0
37	1871377.0
38	2408771.0
39	1414305.0
40	56725.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.2014245318146	20.837509642366978	13.71455746442465	24.24650836139377
2	14.614607740120913	21.564073055136827	41.55430972941685	22.26700947532541
3	17.403345656999132	27.514401018442786	31.414324631046362	23.66792869351172
4	11.84127429103147	18.66668788196034	39.490280237629435	30.00175758937876
5	12.30290133051841	38.6559443301626	36.27954379893086	12.761610540388132
6	28.63644552493156	38.97671626804571	19.26880160805737	13.118036598965361
7	25.27473091135847	33.394061882615034	23.993799574641944	17.337407631384544
8	24.349618487300127	36.439893129756605	21.859294351983216	17.351194030960045
9	25.764268478669237	16.04268989509251	21.622727272251453	36.5703143539868
10-11	23.737102467283993	26.887933320304118	29.597808747569974	19.777155464841904
12-13	24.79906348788791	24.704741275758035	29.261198675626325	21.23499656072773
14-15	22.023297897942598	25.888609077954406	27.092167711953643	24.995925312149353
16-17	21.25841795670253	28.716791057763547	27.946137638777046	22.078653346756877
18-19	20.970990796659425	27.945889168611227	29.759371329254986	21.32374870547436
20-21	22.00120995314703	27.259177658976203	29.40566678489131	21.33394560298546
22-23	21.62938043214629	27.537469942143485	29.14343491601442	21.6897147096958
24-25	21.27808920736131	27.701565892556662	29.584726519269754	21.435618380812272
26-27	21.296246529555628	27.80125196355985	29.352549372011833	21.549952134872687
28-29	21.031753978828164	27.836321060913193	29.3569779181577	21.77494704210094
30-31	21.358622501247734	27.631857942157662	29.373043683837242	21.63647587275736
32-33	21.12317715922322	28.00446224301555	29.355197972474922	21.517162625286307
34-35	21.359334660992253	28.23493040396378	29.41422772753172	20.991507207512242
36-37	21.410124696732744	28.034092103077988	28.97495974909911	21.580823451090154
38-39	21.28132606081142	27.97688965338257	29.2214394435215	21.520344842284512
40-41	21.53609798502809	27.81335662256132	29.26216802767595	21.38837736473464
42-43	21.662677495994252	27.848483341621083	28.942256478258464	21.546582684126196
44-45	21.45448749926824	28.46338432183961	28.570119303077096	21.512008875815056
46-47	21.761628555239763	28.05017897086632	28.03186659900741	22.156325874886505
48-49	21.724198233285055	28.050304607418948	28.189957490219214	22.03553966907678
50-51	21.614968999316524	28.26395996865591	28.004135181025713	22.11693585100186
52-53	21.54748825385949	28.407412138454045	28.079299674252624	21.965799933433843
54-55	21.75773920356442	28.322579739321647	27.87599537647059	22.04368568064334
56-57	21.956439512930334	28.462748174346313	27.540295022740352	22.040517289983
58-59	21.630386280788013	28.605511255109416	28.133018873222177	21.631083590880394
60-61	21.998252290345313	28.940017226528852	27.486668232714745	21.575062250411086
62-63	21.75628062629723	28.87062636907699	27.508052402915112	21.86504060171067
64-65	21.615152158924047	28.73381979414854	27.632947052796148	22.018080994131264
66-67	21.774376685122615	28.69595465559835	27.476857868193548	22.052810791085488
68-69	21.80114064613831	28.409523255196145	27.636612813352624	22.15272328531292
70-71	21.877354739437724	27.97837671204207	27.817754946517415	22.32651360200279
72-73	21.916019908557402	28.048290353769573	27.830893943379536	22.204795794293485
74-75	21.906369962341547	28.100400929002628	27.74913910457373	22.2440900040821
76-77	21.905720681792978	28.095406715201936	27.742637814001338	22.256234789003752
78-79	21.83952741619629	28.05252851054026	27.691294205736668	22.416649867526782
80-81	21.832212533211244	27.996235083483313	27.826753828733626	22.344798554571824
82-83	22.114260723360974	27.8664097935254	27.99491829935375	22.02441118375987
84-85	22.311584160318077	27.95489556460647	27.624008378491677	22.10951189658378
86-87	21.869351089379396	28.019088995696144	27.854108785705805	22.25745112921865
88-89	22.106100753393417	27.80209914254663	27.85982845922223	22.23197164483772
90-91	22.17365578270594	27.68070210250973	27.972433319633698	22.173208795150632
92-93	22.200726207513096	27.735752634465882	27.970966691642747	22.092554466378274
94-95	21.799013434403957	27.884865780998307	28.080158287961254	22.23596249663648
96-97	21.856533860149423	28.028940653851397	28.126164531341928	21.98836095465725
98-99	22.88063140721531	30.06173662245929	28.333062178325484	18.724569791999915
100	29.301040982311243	41.41754458019917	29.281414437489587	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1895.0
1	2004.0
2	3893.5
3	5970.5
4	8704.5
5	15341.5
6	20349.0
7	22429.0
8	22373.0
9	19902.0
10	17087.0
11	14781.0
12	16195.5
13	22931.0
14	37320.5
15	56102.5
16	70721.5
17	77918.0
18	78127.0
19	73471.0
20	66153.0
21	58874.5
22	55166.5
23	56938.5
24	65307.0
25	80256.0
26	100303.5
27	120475.5
28	139225.5
29	161899.5
30	183962.5
31	203641.0
32	229095.5
33	256980.5
34	282262.0
35	309027.0
36	333918.5
37	355441.0
38	368056.0
39	372011.5
40	380084.5
41	385336.0
42	390050.0
43	400803.5
44	414950.5
45	432647.0
46	447438.5
47	456921.5
48	452186.5
49	434094.5
50	416480.0
51	398395.5
52	377550.5
53	351877.5
54	322640.0
55	294107.0
56	266864.0
57	239175.5
58	210340.5
59	174260.0
60	135634.0
61	104212.5
62	78803.0
63	59100.0
64	43053.0
65	31028.0
66	22668.5
67	17235.5
68	13082.5
69	9692.5
70	6959.0
71	4676.5
72	3347.5
73	2239.5
74	1493.0
75	989.0
76	671.5
77	467.5
78	329.0
79	250.0
80	189.0
81	143.5
82	108.0
83	67.0
84	39.5
85	29.5
86	24.0
87	20.0
88	13.5
89	8.0
90	4.5
91	3.0
92	3.5
93	2.0
94	0.5
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008583787131291012
3	0.0028263689334738704
4	3.7684919112984937E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.4655246321716364E-4
16-17	2.6693484371697663E-4
18-19	0.001737693492432083
20-21	3.1405591807495447E-5
22-23	0.001560028999787407
24-25	3.508315913061209E-4
26-27	0.002661140156070632
28-29	0.005866100361690433
30-31	0.002973459613956684
32-33	0.002236346013151508
34-35	0.0022059403321774947
36-37	0.002601330919953469
38-39	0.0
40-41	6.657970920056816E-4
42-43	0.006292769353870708
44-45	0.004111701830586793
46-47	5.518143114122538E-4
48-49	0.01601238306355288
50-51	0.011106093105943932
52-53	0.01565215494791821
54-55	0.013474677864607924
56-57	0.011670024966190982
58-59	0.017997234213180294
60-61	0.009320249406867594
62-63	0.021435838853602184
64-65	0.015387140520027261
66-67	0.03288435127415707
68-69	0.023102878009997368
70-71	0.02280992502364758
72-73	0.021210282111213636
74-75	0.010668780207686985
76-77	0.012860598345364207
78-79	0.013699021624945839
80-81	0.010639931119983737
82-83	0.00549901925475267
84-85	0.003502009419724603
86-87	0.015113401272109984
88-89	0.0015579000603196985
90-91	2.3945704551671358E-4
92-93	0.0011569907745466591
94-95	0.0034759324188713627
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	255.0
20-21	1111.0
22-23	2057.0
24-25	3533.0
26-27	5082.0
28-29	14907.0
30-31	35579.0
32-33	47226.0
34-35	62225.0
36-37	87858.0
38-39	105064.0
40-41	109646.0
42-43	119376.0
44-45	135677.0
46-47	132892.0
48-49	122142.0
50-51	106889.0
52-53	100549.0
54-55	109704.0
56-57	117418.0
58-59	119876.0
60-61	119430.0
62-63	117699.0
64-65	122647.0
66-67	129886.0
68-69	123115.0
70-71	114038.0
72-73	104923.0
74-75	104051.0
76-77	104517.0
78-79	112018.0
80-81	114770.0
82-83	109165.0
84-85	109128.0
86-87	111879.0
88-89	120316.0
90-91	127265.0
92-93	134883.0
94-95	154712.0
96-97	326140.0
98-99	1813412.0
100-101	3739833.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.8077532193976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.69166676485995	71.6697432055185
2	6.936643176497741	11.210690999548953
3	2.006326727821365	4.86380265297811
4	0.9286899307145164	3.001813869540724
5	0.4969410938606066	2.00783466386327
6	0.30078854081728484	1.4583627706551516
7	0.17722519504698173	1.0024818877931274
8	0.12338505613084759	0.7976375333426442
9	0.07506103152471835	0.5458961980658578
>10	0.25930635183296297	3.0183073655460695
>50	0.0028880666823016704	0.15831816821037925
>100	9.073627871899867E-4	0.12939773931350507
>500	1.2192981190822704E-4	0.07549256535793472
>1k	4.8771611707208695E-5	0.0602203802658512
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.046803308694026E-5	0.0	0.0	0.0	0.0
3	1.046803308694026E-5	0.0	0.0	0.0	0.0
4	1.046803308694026E-5	0.0	0.0	4.187213234776104E-5	0.0
5	3.140409926082078E-5	0.0	0.0	5.23401654347013E-5	0.0
6	3.140409926082078E-5	0.0	0.0	5.23401654347013E-5	0.0
7	5.23401654347013E-5	0.0	0.0	5.23401654347013E-5	0.0
8	6.280819852164156E-5	0.0	0.0	5.23401654347013E-5	0.0
9	6.280819852164156E-5	0.0	0.0	5.23401654347013E-5	0.0
10-11	6.280819852164156E-5	0.0	0.0	1.3608443013022337E-4	0.0
12-13	6.80422150651117E-5	0.0	0.0	1.3608443013022337E-4	0.0
14-15	1.046803308694026E-4	0.0	0.0	1.5178647976063378E-4	0.0
16-17	1.046803308694026E-4	0.0	0.0	1.6225451284757403E-4	0.0
18-19	1.2561639704328312E-4	0.0	0.0	1.9365861210839482E-4	0.0
20-21	1.4655246321716364E-4	0.0	0.0	3.6114714149943893E-4	0.0
22-23	1.8842459556492469E-4	0.0	0.0	0.001041569292150556	0.0
24-25	2.4076476099962597E-4	0.0	0.0	0.0028787090989085716	0.0
26-27	2.4076476099962597E-4	0.0	0.0	0.007275282995423481	0.0
28-29	3.0880697606473767E-4	0.0	0.0	0.02427013471207099	0.0
30-31	3.2450902569514806E-4	0.0	0.0	0.026389911412176396	0.0
32-33	3.3497705878208833E-4	0.0	0.0	0.03071844309362619	0.0
34-35	3.716151745863792E-4	0.0	0.0	0.03619845841463942	0.0
36-37	3.9778525730372986E-4	0.0	0.0	0.045389391464972964	0.0
38-39	4.6059345582537145E-4	0.0	0.0	0.06898957205947978	0.0
40-41	4.710614889123117E-4	0.0	0.0	0.09440072237802727	0.0
42-43	5.234016543470129E-4	0.0	0.0	0.11058430153043691	0.0
44-45	5.757418197817142E-4	0.0	0.0	0.13019616151881946	0.0
46-47	6.647201010207065E-4	0.0	0.0	0.15015870061561454	0.0
48-49	6.908901837380571E-4	0.0	0.0	0.16815324949206487	0.0
50-51	6.908901837380571E-4	0.0	0.0	0.18692243281694876	0.0
52-53	6.908901837380571E-4	0.0	0.0	0.2271092118377124	0.0
54-55	7.432303491727584E-4	0.0	0.0	0.27257187953429396	0.0
56-57	8.269746138682805E-4	0.0	0.0	0.3212534674051096	0.0
58-59	8.269746138682805E-4	0.0	0.0	0.36408342478032574	0.0
60-61	8.531446965856311E-4	0.0	0.0	0.3828369060555792	0.0
62-63	8.583787131291013E-4	0.0	0.0	0.4048773497201319	0.0
64-65	8.793147793029818E-4	0.0	0.0	0.41495806558285536	0.0
66-67	9.316549447376831E-4	0.0	0.0	0.4226939420341042	0.0
68-69	9.421229778246233E-4	0.0	0.0	0.43034607422065757	0.0
70-71	9.944631432593247E-4	0.0	0.0	0.43700897728049504	0.0
72-73	0.0010153992094332053	0.0	0.0	0.4425727368662038	0.0
74-75	0.0010258672425201454	0.0	0.0	0.44685416239876236	0.0
76-77	0.0010311012590636156	0.0	0.0	0.4511722260471252	0.0
78-79	0.001041569292150556	0.0	0.0	0.45383110645120806	0.0
80-81	0.0011200795403026077	0.0	0.0	0.4547522933628588	0.0
82-83	0.0011410156064764884	0.0	0.0	0.45531233313301006	0.0
84-85	0.0011462496230199583	0.0	0.0	0.4554850556789446	0.0
86-87	0.001167185689193839	0.0	0.0	0.45563684215870526	0.0
88	0.0011828877388242493	0.0	0.0	0.4556420761752487	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17615	0.0	37.711403	1
GTATCAA	28810	0.0	30.720186	1
TGCGCGA	960	0.0	28.709984	94
CTTATAC	12895	0.0	26.145197	1
ATCAACG	34220	0.0	25.242489	3
AACGCAG	34650	0.0	25.078222	6
CAACGCA	34765	0.0	24.834227	5
TCAACGC	34985	0.0	24.74664	4
TATCAAC	35535	0.0	24.703808	2
TATACAC	15015	0.0	24.503094	3
CGCAGAG	38840	0.0	22.269863	8
ACGCAGA	39115	0.0	22.10307	7
TACATGG	35145	0.0	21.848589	2
GTACATG	36775	0.0	21.489185	1
ACATGGG	35555	0.0	21.21287	3
CGACGGG	730	1.8189894E-12	20.97533	94
TTATACA	17115	0.0	20.656706	2
ACACATC	17920	0.0	19.03493	6
GCAGAGT	45555	0.0	18.855518	9
CATGGGG	21890	0.0	18.5239	4
>>END_MODULE