FastQCFastQC Report
Fri 27 May 2016
SRR2139879_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139879_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17186661
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA233050.039.482771
GTATCAA389250.030.118761
CTTATAC152200.028.6052821
ATCAACG450350.025.3454763
AACGCAG478450.023.9157356
CAACGCA481000.023.7471285
TCAACGC481300.023.7072934
ACGCAGA482000.023.6895967
TATACAC199300.023.4972933
TATCAAC494100.023.3777712
ACATGGG554250.022.173913
TACATGG586750.021.6440162
GTACATG598500.021.6084371
TTATACA208050.020.9455182
CGCAGAG566000.020.1809228
ACACATC223400.019.1287366
CATGGGG405050.018.8197634
TACACAT258450.017.9020395
TATGCCG130200.017.3712142-43
ATACACA317850.017.2605674
Produced by FastQC (version 0.11.5)