##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139879_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17186661
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27333773558459	33.0	31.0	34.0	30.0	34.0
2	32.38628143069791	34.0	31.0	34.0	30.0	34.0
3	33.0763287295886	34.0	31.0	34.0	31.0	37.0
4	35.92796652008206	37.0	35.0	37.0	35.0	37.0
5	35.827493950104675	37.0	35.0	37.0	35.0	37.0
6	35.8358414121277	37.0	35.0	37.0	35.0	37.0
7	35.814275268477104	37.0	35.0	37.0	33.0	37.0
8	36.0453627379978	37.0	35.0	37.0	33.0	39.0
9	37.335806006763036	39.0	37.0	39.0	34.0	39.0
10-11	37.25534689373346	39.0	37.0	39.0	33.0	39.0
12-13	37.35485063096316	39.0	37.0	39.0	33.0	39.5
14-15	38.38881083416959	40.0	38.0	41.0	33.0	41.0
16-17	38.38830963152179	40.0	38.0	41.0	33.0	41.0
18-19	38.39574065608207	40.0	38.0	41.0	33.5	41.0
20-21	38.359430391609	40.0	38.0	41.0	34.0	41.0
22-23	38.25393528688657	40.0	38.0	41.0	33.0	41.0
24-25	38.13961033662734	40.0	37.5	41.0	33.0	41.0
26-27	37.96962226170901	40.0	37.0	41.0	33.0	41.0
28-29	37.831522181234504	40.0	37.0	41.0	33.0	41.0
30-31	37.695514293930195	40.0	37.0	41.0	32.0	41.0
32-33	37.867099659129515	40.0	37.0	41.0	33.0	41.0
34-35	37.84947763107823	40.0	37.0	41.0	33.0	41.0
36-37	37.75569153515572	40.0	37.0	41.0	33.0	41.0
38-39	37.644940194039805	40.0	37.0	41.0	32.5	41.0
40-41	37.59980296748431	40.0	37.0	41.0	32.0	41.0
42-43	37.48704828577519	39.5	36.0	41.0	32.0	41.0
44-45	37.55983981296647	39.5	36.5	41.0	32.5	41.0
46-47	37.65781706637409	40.0	37.0	41.0	32.5	41.0
48-49	37.574312199930446	40.0	36.5	41.0	32.0	41.0
50-51	37.431060369251284	39.5	36.0	41.0	31.5	41.0
52-53	37.291927296099814	39.0	36.0	41.0	31.0	41.0
54-55	37.16240259078355	39.0	35.5	41.0	31.0	41.0
56-57	36.97952332132266	39.0	35.0	40.0	31.0	41.0
58-59	36.81382852916148	38.5	35.0	40.0	31.0	41.0
60-61	36.55663430959797	38.0	35.0	40.0	31.0	41.0
62-63	36.28657569703876	38.0	35.0	40.0	30.0	41.0
64-65	36.04727470760962	37.0	34.0	40.0	30.0	41.0
66-67	35.73850166909399	37.0	34.0	39.5	29.5	41.0
68-69	35.40405486438594	36.0	34.0	39.0	29.0	41.0
70-71	35.09599817633119	36.0	34.0	39.0	29.0	40.0
72-73	34.76915028795945	35.5	34.0	38.5	29.0	40.0
74-75	34.289541705917145	35.0	33.0	37.5	28.5	39.5
76-77	33.44948921538014	34.5	32.0	36.0	27.5	39.0
78-79	33.7263575605911	35.0	33.0	36.0	28.5	39.0
80-81	33.5944773309058	35.0	33.0	36.0	29.0	38.0
82-83	33.373643910474165	35.0	33.0	36.0	29.0	37.0
84-85	33.17010217753666	35.0	32.5	35.0	28.0	37.0
86-87	32.9697496429743	34.5	32.0	35.0	28.0	36.0
88-89	32.769235784381095	34.0	32.0	35.0	27.0	36.0
90-91	32.64154512682942	34.0	32.0	35.0	27.0	36.0
92-93	32.56148578826476	34.0	32.0	35.0	27.0	35.0
94-95	32.52143936398406	34.0	32.0	35.0	27.0	35.0
96-97	32.480206736730494	34.0	32.0	35.0	27.0	35.0
98-99	32.600082760753935	34.0	32.0	35.0	28.5	35.0
100-101	31.509914311689354	33.0	30.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	1.0
17	3.0
18	4.0
19	36.0
20	165.0
21	877.0
22	3625.0
23	12235.0
24	30995.0
25	63893.0
26	112593.0
27	175676.0
28	247424.0
29	326816.0
30	412445.0
31	510979.0
32	634996.0
33	806320.0
34	1073513.0
35	1473764.0
36	2241935.0
37	3490424.0
38	3597571.0
39	1868558.0
40	101811.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.710479357508305	0.008791739232941407	24.354828311471714	31.925900591787038
2	14.824792699537259	24.91661637048602	36.95733873399786	23.30125219597886
3	16.15438239298423	25.076953628365832	33.079822599507324	25.688841379142612
4	13.108829646686823	23.662421853462536	36.39356820671334	26.835180293137302
5	17.25566123635068	36.884599050391465	32.221209227318795	13.638530485939066
6	28.52181118833961	37.11360222907754	20.133294070325817	14.231292512257035
7	26.254168858046366	32.418536677950414	22.715348839428437	18.61194562457478
8	24.786990329302476	31.808080697001007	21.99551151907866	21.409417454617856
9	24.25084779411196	19.86734945199652	23.330029026580558	32.551773727310966
10-11	25.217216421502698	25.673052491115055	28.472694608917926	20.637036478464317
12-13	25.187388143578627	23.769532089814113	28.26513399671245	22.777945769894814
14-15	22.687785559611086	25.929209072554084	25.94619620466332	25.436809163171514
16-17	22.083762663637795	27.939719886905547	27.274341679067387	22.70217577038927
18-19	22.08524443424014	27.40664889434222	28.538361787497003	21.96974488392064
20-21	22.771993685553042	26.975305657090665	28.236754869102775	22.01594578825352
22-23	22.601151105856758	26.803897675612575	27.963916299600616	22.631034918930045
24-25	22.342706090705544	27.064236752397537	28.36549338410461	22.22756377279231
26-27	22.409603997443362	27.365368012008634	27.99784811287653	22.227179877671478
28-29	22.249154889372434	27.14939912230021	28.214634129282146	22.38681185904521
30-31	22.583166135274357	27.136289504620574	28.068952287762084	22.21159207234298
32-33	22.251406001294868	27.35343497657558	27.95867442477476	22.436484597354788
34-35	22.451038186707724	27.13950326759514	28.223264537796677	22.186194007900454
36-37	22.63619445786109	27.197018780620297	28.04711830932153	22.119668452197082
38-39	22.135689262474752	27.41398848402555	28.143877647750877	22.306444605748823
40-41	22.524619766405028	27.038827030252	28.022149670272974	22.41440353307
42-43	22.63013699468287	27.000827373789548	27.874475357981805	22.494560273545776
44-45	22.300616166487792	27.69492092440743	27.61311693134769	22.391345977757084
46-47	22.68349084338837	27.130399291855344	27.209793386149165	22.976316478607124
48-49	22.4756787600774	27.34381903556617	27.3910843903512	22.789417814005233
50-51	22.50654389805235	27.381438163826683	27.193678024674156	22.918339913446808
52-53	22.62857836577485	27.299083291107472	27.228048945896848	22.844289397220834
54-55	22.577673759353363	27.362438401920812	27.201613638189272	22.858274200536556
56-57	22.74930864695736	27.70816436945277	26.70812123081167	22.834405752778196
58-59	22.674190868673147	27.5098030556881	27.223401829421405	22.59260424621735
60-61	22.765189076425557	27.699298019025026	26.97999389581761	22.55551900873181
62-63	22.650550333658604	27.8090986766202	26.932953312086045	22.607397677635156
64-65	22.675358811440418	27.50853230858093	26.897910531677667	22.918198348300983
66-67	22.541047270676977	27.705814022285125	26.84724295032378	22.905895756714116
68-69	22.695351816817816	27.52453034709969	26.956391696478292	22.823726139604204
70-71	22.875928626627196	26.97473042221794	26.956313895117635	23.19302705603723
72-73	22.662285868380604	27.343008622254484	27.00526400200073	22.989441507364187
74-75	22.584459604191117	27.40440378583483	27.12818096570556	22.882955644268492
76-77	22.874797812683386	27.183127585392437	26.84653581298557	23.0955387889386
78-79	22.486071248253634	27.36514474516849	27.11327288788124	23.035511118696636
80-81	22.661854516153955	27.28898039653103	27.044463008807025	23.00470207850799
82-83	22.949116012220063	27.06478780693668	27.086718016165673	22.899378164677582
84-85	22.828830113654515	27.242530611053052	27.02069344638762	22.907945828904808
86-87	22.70808595045769	27.341193020166266	27.023246653794065	22.92747437558198
88-89	22.827764896819986	27.15607626303573	26.877087055151623	23.13907178499267
90-91	22.759937503200618	27.172098059242472	27.15108355316362	22.916880884393283
92-93	22.776429189731115	27.0915255623801	27.220362664628194	22.911682583260593
94-95	22.667403882510225	26.907112886420602	27.289550310957907	23.135932920111262
96-97	22.46101079543215	27.26436279225797	27.43614880076961	22.838477611540274
98-99	22.788218579588257	28.229034651292434	27.405153077355955	21.57759369176336
100-101	25.99665031091008	35.827249906212366	28.65875446361872	9.517345319258833
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3093.0
1	3218.5
2	5696.0
3	8561.0
4	12047.5
5	19020.5
6	23903.0
7	26590.5
8	27440.0
9	25599.0
10	23089.5
11	21462.0
12	24677.0
13	33423.5
14	52188.5
15	78709.0
16	99594.0
17	107558.5
18	104453.0
19	96918.5
20	88045.5
21	80016.0
22	76922.5
23	81751.5
24	94967.0
25	114822.5
26	140921.5
27	168694.0
28	197752.5
29	233987.5
30	269977.5
31	305257.0
32	347581.0
33	391707.5
34	434806.5
35	477104.0
36	518696.5
37	559724.5
38	587848.5
39	604010.0
40	621821.0
41	635271.0
42	644136.5
43	667067.0
44	694206.5
45	716809.5
46	751541.0
47	784278.5
48	794717.5
49	795694.5
50	787026.5
51	766827.5
52	744711.5
53	721436.0
54	700229.5
55	670869.0
56	628595.0
57	573956.0
58	515213.0
59	443758.0
60	362635.5
61	293247.5
62	232499.0
63	179258.5
64	134952.0
65	101311.5
66	75377.0
67	58547.5
68	46017.0
69	34490.0
70	25417.0
71	17456.0
72	12058.0
73	8189.0
74	5484.0
75	3720.0
76	2543.5
77	1734.0
78	1241.5
79	889.0
80	606.0
81	414.5
82	306.5
83	217.5
84	156.0
85	112.5
86	84.5
87	66.5
88	44.5
89	29.5
90	17.0
91	8.5
92	7.0
93	5.0
94	3.5
95	3.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.24748006608148E-4
2	7.796744230889292E-4
3	6.98215901273668E-5
4	2.2692016791394208E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	4.654772675157787E-5
14-15	7.127620658835361E-4
16-17	0.002883049825675854
18-19	8.145852181526127E-5
20-21	0.002237320336121741
22-23	4.073741115788962E-5
24-25	0.0010186906152486752
26-27	0.003098155566660515
28-29	1.1657199417874433E-5
30-31	2.2193414262007796E-4
32-33	7.911908923261869E-4
34-35	0.0013160773510772358
36-37	0.010768078658041766
38-39	0.013236721240190938
40-41	0.02139516250836401
42-43	0.0019395260073765007
44-45	0.001418940205952665
46-47	0.0017883073425856604
48-49	0.0015428663089767022
50-51	8.724005049841856E-5
52-53	7.035321897063097E-4
54-55	0.0061062671483921365
56-57	0.004239713181554067
58-59	0.003624120672980781
60-61	0.001490662938021771
62-63	5.94938446753009E-4
64-65	7.967628703643228E-4
66-67	6.610188297751211E-4
68-69	1.477392223960251E-5
70-71	3.754643508426002E-6
72-73	1.5263373128381337E-4
74-75	3.179109189567916E-4
76-77	0.0015168127862984135
78-79	0.003145694461942787
80-81	0.011540515595794828
82-83	0.010901567078447768
84-85	0.01321876562032633
86-87	0.016001248373256706
88-89	0.07337059490565394
90-91	3.8216221446188143E-4
92-93	1.3357659627487116E-4
94-95	0.001391608863620722
96-97	0.012903392998478588
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	540.0
20-21	2065.0
22-23	3845.0
24-25	6338.0
26-27	11890.0
28-29	26931.0
30-31	55384.0
32-33	73685.0
34-35	107791.0
36-37	158250.0
38-39	174560.0
40-41	174140.0
42-43	197541.0
44-45	226912.0
46-47	227234.0
48-49	214607.0
50-51	197490.0
52-53	193464.0
54-55	207705.0
56-57	218239.0
58-59	226304.0
60-61	224468.0
62-63	209918.0
64-65	219120.0
66-67	235982.0
68-69	221108.0
70-71	216319.0
72-73	207090.0
74-75	205515.0
76-77	209160.0
78-79	223828.0
80-81	225390.0
82-83	213906.0
84-85	214984.0
86-87	226463.0
88-89	243270.0
90-91	263014.0
92-93	278494.0
94-95	350376.0
96-97	522528.0
98-99	1952627.0
100-101	7818186.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.19409133952942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.20966156779092	69.85683995228614
2	7.097078266919985	11.240933290285009
3	2.1104401998785387	5.014031818673872
4	0.9732373135619966	3.08298578821068
5	0.5226636437919185	2.0695936173154235
6	0.3144210770083159	1.4940174895001872
7	0.20044547638434818	1.111186815576429
8	0.14355261524638951	0.9094815139080716
9	0.09337008844895288	0.6654923381705684
>10	0.3279350673502579	3.885684547465996
>50	0.0051245485399974	0.2730566380850687
>100	0.0018720359332173402	0.2672524212570087
>500	1.4858695028110741E-4	0.07947622546770047
>1k	4.951219486828557E-5	0.0499675437977319
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.818465843947233E-6	0.0
5	2.5019403128973103E-4	0.0	0.0	5.818465843947233E-6	0.0
6	2.5019403128973103E-4	0.0	0.0	1.1636931687894466E-5	0.0
7	4.4802186998393694E-4	0.0	0.0	1.1636931687894466E-5	0.0
8	5.120249942673565E-4	0.0	0.0	1.1636931687894466E-5	0.0
9	5.120249942673565E-4	0.0	0.0	1.1636931687894466E-5	0.0
10-11	5.702096527068288E-4	0.0	0.0	2.327386337578893E-5	0.0
12-13	7.273082304934042E-4	0.0	0.0	2.9092329219736168E-5	0.0
14-15	9.367730008755046E-4	0.0	0.0	5.23661925955251E-5	0.0
16-17	0.0010444146189885284	0.0	0.0	5.8184658439472335E-5	0.0
18-19	0.0013760671720935208	0.0	0.0	7.273082304934042E-5	0.0
20-21	0.0016378981350711462	0.0	0.0	8.72769876592085E-5	0.0
22-23	0.0017571766848720645	0.0	0.0	1.1346008395697106E-4	0.0
24-25	0.0023419325021887616	0.0	0.0	1.6000781070854892E-4	5.818465843947233E-6
26-27	0.002376843297252445	0.0	0.0	3.4328948479288676E-4	5.818465843947233E-6
28-29	0.0028743221269099334	0.0	0.0	0.0010415053860665547	5.818465843947233E-6
30-31	0.0031332438569655853	0.0	0.0	0.0027637712758749357	5.818465843947233E-6
32-33	0.003203065447092952	0.0	0.0	0.0059842921204997295	5.818465843947233E-6
34-35	0.0035027164380562344	0.0	0.0	0.010735069482082644	5.818465843947233E-6
36-37	0.003930373677586356	0.0	0.0	0.017100471115360918	5.818465843947233E-6
38-39	0.004424943274321871	0.0	0.0	0.025464515766035065	5.818465843947233E-6
40-41	0.004520947960747	0.0	0.0	0.034294037684224996	5.818465843947233E-6
42-43	0.004887511308915676	0.0	0.0	0.04204132495544073	5.818465843947233E-6
44-45	0.005425719399480795	0.0	0.0	0.05149924118477696	5.818465843947233E-6
46-47	0.006307216974838801	0.0	0.0	0.06158264249233751	5.818465843947233E-6
48-49	0.0066795987888514236	0.0	0.0	0.07131402661633926	5.818465843947233E-6
50-51	0.006691235720539318	0.0	0.0	0.08090285832716432	5.818465843947233E-6
52-53	0.00670869111807116	0.0	0.0	0.09000584814001975	5.818465843947233E-6
54-55	0.0068163327361841835	0.0	0.0	0.09947540130104388	5.818465843947233E-6
56-57	0.007261445373246147	0.0	0.0	0.10859875574435313	5.818465843947233E-6
58-59	0.007305083867075752	0.0	0.0	0.11782102410700951	5.818465843947233E-6
60-61	0.0073690869913591705	0.0	0.0	0.12710147712810532	5.818465843947233E-6
62-63	0.007500002472847983	0.0	0.0	0.1361230084191455	5.818465843947233E-6
64-65	0.007505820938691931	0.0	0.0	0.1450718088871364	5.818465843947233E-6
66-67	0.007977116672051657	0.0	0.0	0.15436971730576404	5.818465843947233E-6
68-69	0.008151670647370074	0.0	0.0	0.16331269930791095	5.818465843947233E-6
70-71	0.008366953883596122	0.0	0.0	0.17193275645571876	5.818465843947233E-6
72-73	0.008553144790602433	0.0	0.0	0.17894400779767516	5.818465843947233E-6
74-75	0.008660786408715457	0.0	0.0	0.1851174000581032	5.818465843947233E-6
76-77	0.008707334135467034	0.0	0.0	0.1908398612156253	5.818465843947233E-6
78-79	0.009062260551947816	0.0	0.0	0.1962655806151061	5.818465843947233E-6
80-81	0.009414277735506623	0.0	0.0	0.19925045359305102	5.818465843947233E-6
82-83	0.009676108698484249	0.0	0.0	0.20130146280304245	5.818465843947233E-6
84-85	0.010118312102624238	0.0	0.0	0.20284626548461043	5.818465843947233E-6
86-87	0.01015322289768792	0.0	0.0	0.20306154872083648	5.818465843947233E-6
88-89	0.0101997706244395	0.0	0.0	0.20309355028297818	5.818465843947233E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	23305	0.0	39.48277	1
GTATCAA	38925	0.0	30.11876	1
CTTATAC	15220	0.0	28.605282	1
ATCAACG	45035	0.0	25.345476	3
AACGCAG	47845	0.0	23.915735	6
CAACGCA	48100	0.0	23.747128	5
TCAACGC	48130	0.0	23.707293	4
ACGCAGA	48200	0.0	23.689596	7
TATACAC	19930	0.0	23.497293	3
TATCAAC	49410	0.0	23.377771	2
ACATGGG	55425	0.0	22.17391	3
TACATGG	58675	0.0	21.644016	2
GTACATG	59850	0.0	21.608437	1
TTATACA	20805	0.0	20.945518	2
CGCAGAG	56600	0.0	20.180922	8
ACACATC	22340	0.0	19.128736	6
CATGGGG	40505	0.0	18.819763	4
TACACAT	25845	0.0	17.902039	5
TATGCCG	13020	0.0	17.37121	42-43
ATACACA	31785	0.0	17.260567	4
>>END_MODULE