FastQCFastQC Report
Fri 27 May 2016
SRR2139878_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139878_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13360112
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA166850.040.2266161
GTATCAA279800.032.4490171
CTTATAC123850.032.4229161
TATACAC166450.026.791653
ATCAACG331200.026.5941033
TCAACGC332100.026.5344094
CAACGCA335650.026.2292795
AACGCAG339500.026.0892166
TATCAAC350200.025.5737632
TTATACA177200.023.8905982
ACGCAGA389700.022.6757457
CGCAGAG397750.022.2168168
GTACATG378400.021.1479531
ACATCTC195150.020.6611258
TACATGG379850.020.5478482
ACACATC201150.020.3513326
TACACAT227250.019.9853575
ACATGGG382200.019.7870313
GCAGAGT464050.018.8920739
CACATCT225000.018.0844887
Produced by FastQC (version 0.11.5)