##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139878_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13360112
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15621193894183	33.0	31.0	34.0	30.0	34.0
2	32.38296752302675	34.0	31.0	34.0	31.0	34.0
3	32.433536485322875	34.0	31.0	34.0	30.0	34.0
4	36.07190239123744	37.0	35.0	37.0	35.0	37.0
5	35.9291047859479	37.0	35.0	37.0	35.0	37.0
6	35.94820357793408	37.0	35.0	37.0	35.0	37.0
7	35.898067171891974	37.0	35.0	37.0	35.0	37.0
8	35.89940690616965	37.0	35.0	37.0	35.0	37.0
9	37.67923569802409	39.0	37.0	39.0	35.0	39.0
10-11	37.564011589124405	39.0	37.0	39.0	35.0	39.0
12-13	37.529117794820884	39.0	37.0	39.0	35.0	39.0
14-15	38.805307096227935	40.0	38.0	41.0	35.0	41.0
16-17	38.78748969320017	40.0	38.0	41.0	35.0	41.0
18-19	38.76789202815066	40.0	38.0	41.0	34.0	41.0
20-21	38.76059350758459	40.0	38.0	41.0	34.0	41.0
22-23	38.682008116998816	40.0	38.0	41.0	34.0	41.0
24-25	38.6154872713728	40.0	38.0	41.0	34.0	41.0
26-27	38.44456228904744	40.0	38.0	41.0	34.0	41.0
28-29	38.31707247306433	40.0	38.0	41.0	34.0	41.0
30-31	38.18446221215339	40.0	38.0	41.0	33.5	41.0
32-33	38.1022980585786	40.0	37.5	41.0	33.0	41.0
34-35	38.162715625515176	40.0	38.0	41.0	33.0	41.0
36-37	38.12007151291493	40.0	38.0	41.0	33.0	41.0
38-39	38.05205656963028	40.0	37.0	41.0	33.0	41.0
40-41	37.99408160443721	40.0	37.0	41.0	33.0	41.0
42-43	37.97144383483405	40.0	37.0	41.0	33.0	41.0
44-45	38.012128911648624	40.0	37.0	41.0	33.0	41.0
46-47	38.00199325265018	40.0	37.0	41.0	33.0	41.0
48-49	37.92665439680731	40.0	37.0	41.0	33.0	41.0
50-51	37.82910446904078	40.0	37.0	41.0	33.0	41.0
52-53	37.60617141994655	40.0	36.5	41.0	32.0	41.0
54-55	37.4842642620072	39.0	36.0	41.0	32.0	41.0
56-57	37.41034354624075	39.0	36.0	41.0	32.0	41.0
58-59	37.249220837416146	39.0	35.5	41.0	31.5	41.0
60-61	37.04994470180087	39.0	35.0	40.5	31.0	41.0
62-63	36.8011904658065	38.5	35.0	40.0	31.0	41.0
64-65	36.51397425512209	38.0	35.0	40.0	31.0	41.0
66-67	36.23296202733182	37.5	35.0	40.0	30.5	41.0
68-69	35.95344265209426	37.0	34.0	40.0	30.0	41.0
70-71	35.65097970712636	36.5	34.0	39.0	30.0	41.0
72-73	35.33803942388208	36.0	34.0	39.0	30.0	40.5
74-75	34.987261590292846	35.5	34.0	38.5	29.0	40.0
76-77	33.64574311572012	34.5	32.0	36.5	28.0	39.0
78-79	34.36246100261536	35.0	33.5	37.0	29.0	39.0
80-81	34.30363711468688	35.0	34.0	37.0	29.0	39.0
82-83	34.025783876172525	35.0	34.0	36.0	29.0	38.5
84-85	33.843039630600416	35.0	33.0	36.0	29.0	37.0
86-87	33.60885805273536	35.0	33.0	35.5	29.0	37.0
88-89	33.50016580002277	35.0	33.0	35.0	29.0	36.5
90-91	33.31785228879457	35.0	33.0	35.0	29.0	36.0
92-93	33.2115887251098	35.0	33.0	35.0	29.0	36.0
94-95	33.206722773635846	35.0	33.0	35.0	29.0	36.0
96-97	33.24108245674688	35.0	33.0	35.0	29.0	35.0
98-99	33.332193560699224	35.0	33.0	35.0	29.5	35.0
100	32.030678897071304	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	6.0
19	15.0
20	75.0
21	418.0
22	1814.0
23	6580.0
24	17265.0
25	37083.0
26	68237.0
27	109130.0
28	159007.0
29	213783.0
30	272798.0
31	340403.0
32	424071.0
33	535756.0
34	711345.0
35	985008.0
36	1531101.0
37	2647197.0
38	3359937.0
39	1857414.0
40	81668.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.16243935679581	20.48853332966071	13.720057137245556	24.628970176297923
2	14.675041646357457	21.319244928485627	41.18600203351589	22.81971139164103
3	17.828622993579696	26.72861574813145	30.791897552954644	24.650863705334206
4	12.24488237823156	18.405249896108657	38.77460009317287	30.575267632486913
5	12.817632067755122	38.10090813609946	36.09052828299643	12.990931513148993
6	29.57327004444274	38.28275541402647	18.96875565115023	13.175218890380561
7	25.952888718298173	32.63116357108384	23.864365807711792	17.551581902906204
8	24.2813383600377	36.72679540411038	21.66040973309206	17.331456502759856
9	25.545354709601238	16.378088746561406	21.349536590711217	36.72701995312614
10-11	23.940181040398464	26.722897233196846	29.39219746062009	19.944724265784597
12-13	24.865053526497384	24.287756719404747	29.21961657207664	21.62757318202123
14-15	21.974463986529454	25.614455926716783	26.636625501343104	25.774454585410663
16-17	21.760607505222136	28.680621657968658	27.421837101049245	22.13693373575996
18-19	21.71256008608017	27.945812695238477	28.9196749112011	21.421952307480254
20-21	22.395410359663174	27.25760643849082	28.689922885344508	21.657060316501493
22-23	22.103903087779784	27.387818039498825	28.520900226456973	21.987378646264414
24-25	22.005832729322027	27.457509821525285	28.626201035256475	21.910456413896217
26-27	22.007900999235208	27.607685417321175	28.534292084500528	21.850121498943086
28-29	21.833615363322032	27.627970053156826	28.600277263372654	21.938137320148485
30-31	22.085723454874618	27.53500348943449	28.589397937691853	21.789875117999046
32-33	21.670502841628984	27.615846602911137	28.733239808014133	21.98041074744575
34-35	21.80098803933866	27.69335959965234	28.58055706125566	21.925095299753337
36-37	21.894837237709826	27.698050698587217	28.564519295728118	21.84259276797484
38-39	21.861496387443626	27.673804199034308	28.548384765140217	21.916314648381846
40-41	21.9730510355383	27.574335890126793	28.566249792363795	21.88636328197111
42-43	22.042237116906783	27.664779608523443	28.352514813791796	21.94046846077798
44-45	21.946408547593656	27.97502535177025	28.09782993111925	21.98073616951684
46-47	22.15523661837035	27.70992467117848	27.774427698043482	22.36041101240769
48-49	22.128830825814322	27.7577675831935	27.788100784387133	22.325300806605046
50-51	21.983761291857558	27.918660662887635	27.792806952148347	22.304771093106464
52-53	22.094141960936927	27.886420405390595	27.89857150957879	22.120866124093688
54-55	22.07491918158245	27.9489904312681	27.680247969980105	22.295842417169347
56-57	22.238607363984123	28.08543815737635	27.45017541303656	22.22577906560297
58-59	22.039887141263247	28.173249258716922	27.760820901891886	22.026042698127952
60-61	22.247006340533783	28.348128836623548	27.365566669552706	22.03929815328996
62-63	22.05367417888687	28.417347842161096	27.41923147387325	22.109746505078785
64-65	22.03793667605682	28.283520740932488	27.469869004403076	22.20867357860762
66-67	22.0885978257281	28.19969009493303	27.385156742664705	22.32655533667416
68-69	22.161146600663212	28.07643267408604	27.522179250619693	22.240241474631052
70-71	22.193684986584	27.79278323875293	27.609344797213254	22.40418697744982
72-73	22.212408608540073	27.83893187771836	27.626510322650915	22.322149191090652
74-75	22.150543895847708	27.893108268243772	27.590713547711903	22.365634288196617
76-77	22.17399912759727	27.891965024616418	27.519572780496045	22.414463067290267
78-79	22.167088109085025	27.796376050112983	27.572740246548687	22.463795594253305
80-81	22.121312866553296	27.868114264880656	27.596546255583256	22.41402661298279
82-83	22.2622804488184	27.785800789820097	27.69514950166113	22.25676925970037
84-85	22.38282964580314	27.74280087794649	27.5632255374432	22.311143938807177
86-87	22.177372037298134	27.81137312985806	27.65896587024834	22.35228896259546
88-89	22.263054442031642	27.68783959887346	27.646796516375975	22.402309442718924
90-91	22.335892106505504	27.687701803714678	27.639281186789162	22.337124902990656
92-93	22.28011269701986	27.646364411329976	27.733460341431275	22.34006255021888
94-95	22.143578682026607	27.669979708551516	27.75181341936162	22.434628190060252
96-97	22.148030324566502	27.822430273494795	27.87776557185419	22.151773830084515
98-99	23.23122723030139	29.763922761167816	28.078939973303623	18.92591003522717
100	22.237190892430096	30.790382324908666	21.651144728979883	25.321282053681355
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2151.0
1	2140.0
2	3614.5
3	5410.0
4	7662.0
5	13027.5
6	17290.0
7	19537.5
8	20221.0
9	18776.0
10	16625.5
11	14970.0
12	16689.5
13	22768.0
14	35567.0
15	52823.0
16	66400.5
17	73801.0
18	76019.5
19	73916.0
20	69361.0
21	65703.0
22	65413.0
23	70538.5
24	82711.5
25	101569.5
26	125722.5
27	151524.0
28	177684.0
29	207157.0
30	237648.5
31	267540.0
32	298929.5
33	329670.5
34	361345.5
35	392938.0
36	424278.5
37	454760.5
38	476735.5
39	492151.0
40	508419.0
41	521328.5
42	530861.0
43	544814.5
44	557079.0
45	568275.5
46	580065.5
47	590589.5
48	600756.5
49	594112.0
50	575328.5
51	557859.0
52	533898.5
53	500542.0
54	464350.5
55	428197.0
56	392534.0
57	354042.5
58	312758.0
59	271994.0
60	229188.5
61	187849.0
62	150010.5
63	116421.5
64	89300.0
65	67630.0
66	50311.0
67	38469.5
68	29403.0
69	21599.0
70	15973.0
71	11831.0
72	8448.5
73	5793.5
74	3972.5
75	2659.0
76	1741.5
77	1093.5
78	705.5
79	466.0
80	311.5
81	216.0
82	163.0
83	113.0
84	71.5
85	48.0
86	35.5
87	28.5
88	20.0
89	15.0
90	12.0
91	7.0
92	3.5
93	1.0
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0012050797178945805
18-19	6.362222113108035E-5
20-21	0.0
22-23	0.00720903001752513
24-25	0.008187600042588998
26-27	0.02074028744660209
28-29	0.01627256539541903
30-31	0.002049585478844543
32-33	0.006239569337920315
34-35	0.03290338990747689
36-37	0.0056212132875441525
38-39	0.0024732025626519726
40-41	0.0074749250505282885
42-43	0.007646906326491032
44-45	0.008565374420593292
46-47	0.00550054433802508
48-49	0.0011812736889368985
50-51	0.0024707870340296098
52-53	9.423216490959498E-4
54-55	0.005283267062091711
56-57	0.0011577447504092459
58-59	0.0014759772509551497
60-61	8.883376476931757E-4
62-63	0.0016674854166332988
64-65	4.354267466477472E-4
66-67	0.005760337012592844
68-69	0.00428027750450594
70-71	0.0017041780332562935
72-73	0.001501024120613292
74-75	5.557124004989823E-4
76-77	6.878734689944882E-4
78-79	0.0010917741446826894
80-81	0.001265367042447726
82-83	0.006995060083554613
84-85	6.916280308500683E-4
86-87	9.804750773298114E-5
88-89	4.4036323256389646E-4
90-91	3.949202581284195E-4
92-93	0.002611177690097816
94-95	0.003322803897504502
96-97	6.858326016356765E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	262.0
20-21	1124.0
22-23	2382.0
24-25	4047.0
26-27	7634.0
28-29	15853.0
30-31	38779.0
32-33	51152.0
34-35	69183.0
36-37	100442.0
38-39	117590.0
40-41	115956.0
42-43	136941.0
44-45	152434.0
46-47	151203.0
48-49	139961.0
50-51	126968.0
52-53	123823.0
54-55	134875.0
56-57	145574.0
58-59	147996.0
60-61	147280.0
62-63	139804.0
64-65	146452.0
66-67	158664.0
68-69	150733.0
70-71	140356.0
72-73	132787.0
74-75	132947.0
76-77	132976.0
78-79	142202.0
80-81	145156.0
82-83	139850.0
84-85	141213.0
86-87	148081.0
88-89	163821.0
90-91	176301.0
92-93	187573.0
94-95	218874.0
96-97	484685.0
98-99	865368.0
100-101	7480810.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.58715939308365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.80169732090056	70.75689376982699
2	7.583253611526102	12.222257350204622
3	2.300084987093042	5.560719464175172
4	0.9663656908189701	3.115066638321429
5	0.49166298297484745	1.9810861588336504
6	0.2806347224750418	1.3569333066797993
7	0.17289033811877386	0.9752918864851227
8	0.10707236503136801	0.6902926197901832
9	0.06848760084693029	0.4967299085311316
>10	0.22439079720035301	2.5967364823319503
>50	0.0028182960907483425	0.1499905950993622
>100	6.235665477041086E-4	0.09011906802124837
>500	1.7720375539903502E-5	0.007882751699330065
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.4969934383783608E-5	0.0	2.2454901575675412E-5	0.0
3	0.0	1.4969934383783608E-5	0.0	2.2454901575675412E-5	0.0
4	0.0	2.2454901575675412E-5	0.0	2.2454901575675412E-5	0.0
5	0.0	2.2454901575675412E-5	0.0	2.2454901575675412E-5	0.0
6	0.0	2.2454901575675412E-5	0.0	2.2454901575675412E-5	0.0
7	0.0	2.2454901575675412E-5	0.0	2.2454901575675412E-5	0.0
8	0.0	2.2454901575675412E-5	0.0	2.2454901575675412E-5	0.0
9	0.0	4.4909803151350825E-5	0.0	2.9939868767567217E-5	0.0
10-11	0.0	4.4909803151350825E-5	0.0	2.9939868767567217E-5	0.0
12-13	0.0	4.4909803151350825E-5	0.0	2.9939868767567217E-5	0.0
14-15	0.0	7.484967191891805E-5	0.0	2.9939868767567217E-5	0.0
16-17	7.484967191891804E-6	7.484967191891805E-5	0.0	2.9939868767567217E-5	0.0
18-19	7.484967191891804E-6	7.484967191891805E-5	0.0	3.742483595945902E-5	0.0
20-21	7.484967191891804E-6	7.484967191891805E-5	0.0	4.4909803151350825E-5	0.0
22-23	7.484967191891804E-6	8.981960630270166E-5	0.0	4.4909803151350825E-5	0.0
24-25	7.484967191891804E-6	1.1975947507026887E-4	0.0	6.362222113108033E-5	0.0
26-27	7.484967191891804E-6	1.2724444226216067E-4	0.0	1.3098692585810658E-4	0.0
28-29	7.484967191891804E-6	1.2724444226216067E-4	0.0	4.6781044949323775E-4	0.0
30-31	7.484967191891804E-6	1.2724444226216067E-4	0.0	0.0015493882087216034	0.0
32-33	7.484967191891804E-6	1.2724444226216067E-4	0.0	0.0036152391536837416	0.0
34-35	7.484967191891804E-6	1.571843110297279E-4	0.0	0.006444556752218843	0.0
36-37	7.484967191891804E-6	1.871241797972951E-4	0.0	0.01080455014149582	0.0
38-39	7.484967191891804E-6	1.871241797972951E-4	0.0	0.017716917343207902	0.0
40-41	7.484967191891804E-6	1.9460914698918693E-4	0.0	0.02520562701869565	0.0
42-43	7.484967191891804E-6	1.9460914698918693E-4	0.0	0.03213296415479151	0.0
44-45	7.484967191891804E-6	2.2080653216080824E-4	0.0	0.03953933919116846	0.0
46-47	7.484967191891804E-6	2.4700391733242956E-4	0.0	0.04742849461142242	0.0
48-49	7.484967191891804E-6	2.5074640092837545E-4	0.0	0.05597632714456286	0.0
50-51	7.484967191891804E-6	2.6571633531215905E-4	0.0	0.06416113876889655	0.0
52-53	7.484967191891804E-6	2.6945881890810494E-4	0.0	0.07182574517339375	0.0
54-55	7.484967191891804E-6	2.6945881890810494E-4	0.0	0.07940053197158825	0.0
56-57	7.484967191891804E-6	2.6945881890810494E-4	0.0	0.08675076975402601	0.0
58-59	7.484967191891804E-6	2.6945881890810494E-4	0.0	0.09376792649642458	0.0
60-61	7.484967191891804E-6	2.7694378609999676E-4	0.0	0.10070649108330829	0.0
62-63	7.484967191891804E-6	2.9191372048378037E-4	0.0	0.10729700469576903	0.0
64-65	7.484967191891804E-6	3.517934580189148E-4	0.0	0.11401476275049191	0.0
66-67	7.484967191891804E-6	3.517934580189148E-4	0.0	0.12047803192069048	0.0
68-69	7.484967191891804E-6	3.555359416148607E-4	0.0	0.1268701939025661	0.0
70-71	7.484967191891804E-6	3.592784252108066E-4	0.0	0.1328057728857363	0.0
72-73	7.484967191891804E-6	3.6302090880675254E-4	0.0	0.13787683815824298	0.0
74-75	7.484967191891804E-6	3.8173332678648203E-4	0.0	0.14167545900812806	0.0
76-77	7.484967191891804E-6	3.9670326117026563E-4	0.0	0.14554518704633615	0.0
78-79	7.484967191891804E-6	4.1167319555404923E-4	0.0	0.14804890857202396	0.0
80-81	7.484967191891804E-6	4.1541567914999517E-4	0.0	0.14882360267638475	0.0
82-83	7.484967191891804E-6	4.1915816274594106E-4	0.0	0.1493101255438577	0.0
84-85	7.484967191891804E-6	4.1915816274594106E-4	0.0	0.14948976475646314	0.0
86-87	7.484967191891804E-6	4.1915816274594106E-4	0.0	0.1495459020104023	0.0
88	7.484967191891804E-6	4.565829987054001E-4	0.0	0.14954964449399824	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16685	0.0	40.226616	1
GTATCAA	27980	0.0	32.449017	1
CTTATAC	12385	0.0	32.422916	1
TATACAC	16645	0.0	26.79165	3
ATCAACG	33120	0.0	26.594103	3
TCAACGC	33210	0.0	26.534409	4
CAACGCA	33565	0.0	26.229279	5
AACGCAG	33950	0.0	26.089216	6
TATCAAC	35020	0.0	25.573763	2
TTATACA	17720	0.0	23.890598	2
ACGCAGA	38970	0.0	22.675745	7
CGCAGAG	39775	0.0	22.216816	8
GTACATG	37840	0.0	21.147953	1
ACATCTC	19515	0.0	20.661125	8
TACATGG	37985	0.0	20.547848	2
ACACATC	20115	0.0	20.351332	6
TACACAT	22725	0.0	19.985357	5
ACATGGG	38220	0.0	19.787031	3
GCAGAGT	46405	0.0	18.892073	9
CACATCT	22500	0.0	18.084488	7
>>END_MODULE