FastQCFastQC Report
Fri 27 May 2016
SRR2139877_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139877_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6022621
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103900.039.222051
CTTATAC70500.039.1824761
TATACAC91250.033.312583
GTATCAA170700.030.5111221
TTATACA94650.030.2996062
AACGCAG208450.024.7981626
ATCAACG207250.024.7909263
CAACGCA209500.024.5806245
TCAACGC210200.024.5173554
TATCAAC211900.024.4312932
ACACATC118350.023.637766
TACACAT126300.023.3563585
ACATCTC121400.023.0438948
TACATGG240750.022.5098022
GTACATG251300.022.1556191
ACATGGG246150.021.6350333
ACGCAGA238300.021.6263037
ATACACA145500.021.616954
CGCAGAG239050.021.5094188
CACATCT129450.021.4599697
Produced by FastQC (version 0.11.5)