##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139877_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6022621
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14876579482588	33.0	31.0	34.0	30.0	34.0
2	32.366512187966	34.0	31.0	34.0	30.0	34.0
3	32.41311050454611	34.0	31.0	34.0	30.0	34.0
4	36.057553845742575	37.0	35.0	37.0	35.0	37.0
5	35.91820471518962	37.0	35.0	37.0	35.0	37.0
6	35.942540465355535	37.0	35.0	37.0	35.0	37.0
7	35.88927877082088	37.0	35.0	37.0	35.0	37.0
8	35.8910539115777	37.0	35.0	37.0	35.0	37.0
9	37.67070848389763	39.0	37.0	39.0	35.0	39.0
10-11	37.5514472851604	39.0	37.0	39.0	35.0	39.0
12-13	37.519955016262855	39.0	37.0	39.0	35.0	39.0
14-15	38.782931052775865	40.0	38.0	41.0	34.5	41.0
16-17	38.76235180663037	40.0	38.0	41.0	34.5	41.0
18-19	38.7641257020822	40.0	38.0	41.0	34.0	41.0
20-21	38.76773180263987	40.0	38.0	41.0	34.0	41.0
22-23	38.68937790567385	40.0	38.0	41.0	34.0	41.0
24-25	38.62189638054059	40.0	38.0	41.0	34.0	41.0
26-27	38.460116277518786	40.0	38.0	41.0	34.0	41.0
28-29	38.341754823679636	40.0	38.0	41.0	34.0	41.0
30-31	38.20542288037772	40.0	38.0	41.0	34.0	41.0
32-33	38.09792865296699	40.0	37.0	41.0	33.0	41.0
34-35	38.13309294131927	40.0	38.0	41.0	33.0	41.0
36-37	38.099978206731166	40.0	37.0	41.0	33.0	41.0
38-39	38.02003026320955	40.0	37.0	41.0	33.0	41.0
40-41	37.980330119697626	40.0	37.0	41.0	33.0	41.0
42-43	37.959144403658314	40.0	37.0	41.0	33.0	41.0
44-45	38.005678322159184	40.0	37.0	41.0	33.0	41.0
46-47	38.00257597860961	40.0	37.0	41.0	33.0	41.0
48-49	37.9313050303023	40.0	37.0	41.0	33.0	41.0
50-51	37.83937862522182	40.0	37.0	41.0	33.0	41.0
52-53	37.626227254976676	40.0	36.5	41.0	32.0	41.0
54-55	37.50143174545526	39.0	36.0	41.0	32.0	41.0
56-57	37.43045343824791	39.0	36.0	41.0	32.0	41.0
58-59	37.26650785916574	39.0	35.5	41.0	32.0	41.0
60-61	37.054058502782254	39.0	35.0	40.5	31.0	41.0
62-63	36.80613637026508	38.5	35.0	40.0	31.0	41.0
64-65	36.52621432896139	38.0	35.0	40.0	31.0	41.0
66-67	36.24860469698903	37.0	35.0	40.0	31.0	41.0
68-69	35.97852251996401	37.0	34.5	39.5	30.5	41.0
70-71	35.683646102373686	36.5	34.0	39.0	30.0	41.0
72-73	35.37475589485252	36.0	34.0	39.0	30.0	40.5
74-75	35.02809261834149	35.5	34.0	38.5	30.0	40.0
76-77	33.67027211816136	34.5	32.0	36.5	28.5	39.0
78-79	34.380778782716355	35.0	33.5	37.0	29.0	39.0
80-81	34.3268396680819	35.0	34.0	36.5	30.0	39.0
82-83	34.05504692845231	35.0	34.0	36.0	29.5	37.5
84-85	33.881988455576874	35.0	34.0	36.0	30.0	37.0
86-87	33.66652522494255	35.0	33.0	35.5	29.0	37.0
88-89	33.565645953704546	35.0	33.0	35.0	29.0	36.0
90-91	33.393608180039024	35.0	33.0	35.0	29.0	36.0
92-93	33.29174725291172	35.0	33.0	35.0	29.0	36.0
94-95	33.291080285616914	35.0	33.0	35.0	29.0	36.0
96-97	33.32009505472912	35.0	33.0	35.0	29.5	35.0
98-99	33.405826553621566	35.0	33.0	35.0	30.0	35.0
100	32.16907543570855	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	8.0
20	36.0
21	194.0
22	856.0
23	2838.0
24	7483.0
25	15922.0
26	29036.0
27	46301.0
28	67131.0
29	90034.0
30	115938.0
31	146334.0
32	183654.0
33	233995.0
34	313197.0
35	433187.0
36	673068.0
37	1169101.0
38	1497090.0
39	954605.0
40	42610.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.98230305377011	20.290268970934747	13.139594870738172	24.58783310455697
2	15.086969610075082	21.47315927733125	40.587146360363704	22.852724752229967
3	17.75173964956453	27.08812326062025	31.083526590831468	24.076610498983747
4	12.04638312787738	18.348356969498827	38.66755022439566	30.93770967822813
5	12.376123285858434	38.75767709772872	36.099033958802984	12.767165657609869
6	29.20118998024282	38.22510166254858	19.03237809584897	13.54133026135963
7	25.9063620307504	32.93147617955704	23.467656357589163	17.694505432103398
8	25.03112847379903	35.813360993494356	21.66892122217221	17.486589310534402
9	26.237463722190057	15.620757142114705	20.988685822999653	37.15309331269558
10-11	24.115082121222635	26.521169105610333	29.28751452233172	20.07623425083531
12-13	25.100217994789976	24.215644650393905	28.947828528476222	21.736308826339894
14-15	22.1924391390393	25.41879191800381	26.656683194908	25.7320857480489
16-17	21.687185001080525	28.486035087684343	27.42122697223871	22.405552938996426
18-19	21.555866568326266	27.64296191456938	29.11805131921035	21.683120197894006
20-21	22.493174780174495	26.879672140375487	28.79931840373835	21.827834675711667
22-23	22.10672390797666	27.251607462108616	28.5242987550613	22.11736987485342
24-25	21.857701314615987	27.279326264601572	29.05402088490691	21.808951535875526
26-27	21.797976292227204	27.429228903975112	28.84454278080466	21.92825202299302
28-29	21.465725559407375	27.53776902876004	28.969278253452934	22.027227158379652
30-31	21.919882793538502	27.241856220315064	28.559503440004974	22.278757546141456
32-33	21.846224087808974	27.441158731698017	28.871978798770037	21.840638381722965
34-35	22.06476636113253	27.86615712574975	28.6382452434015	21.43083126971621
36-37	21.815390387718928	27.631620181029316	28.53235061845865	22.020638812793106
38-39	21.64336762344259	27.57448683865177	28.755126199164067	22.027019338741578
40-41	21.877468026656146	27.382051027217358	28.86432818414472	21.87615276198178
42-43	22.100783620675042	27.38547299779055	28.48477752914385	22.02896585239056
44-45	21.700810452207072	28.082233558634357	28.27626237582909	21.940693613329476
46-47	22.207548976027752	27.580491009141024	27.691186765277454	22.52077324955377
48-49	22.186097134566214	27.67906258042643	27.609175988823342	22.52566429618402
50-51	21.952458723033907	27.62035048973343	27.86844574548238	22.55874504175028
52-53	22.012713616950876	27.740677140458697	28.05656445119822	22.19004479139221
54-55	21.962256452106498	27.843595012216866	27.670285578272928	22.523862957403708
56-57	22.471006693708926	28.092810165404174	27.099354633914846	22.336828506972054
58-59	21.94078101557989	28.28291691946744	27.820671775526367	21.955630289426303
60-61	22.330550537295426	28.606689402937274	27.156656707153832	21.90610335261347
62-63	22.001700501512655	28.73248113993962	27.125392733634236	22.140425624913494
64-65	21.983746103957053	28.49223270301003	27.21380305700199	22.310218136030933
66-67	22.072929155882807	28.433793028291614	27.131575391726443	22.361702424099136
68-69	22.239869606715537	28.013708396205356	27.381531922752206	22.364890074326905
70-71	22.322124800114988	27.64863359505543	27.424638859442656	22.604602745386924
72-73	22.29072528758488	27.8073609844511	27.454648098499963	22.44726562946405
74-75	22.27947022960954	27.792204683613093	27.399198184475672	22.529126902301698
76-77	22.29230731964677	27.750697296304104	27.44884376722563	22.508151616823497
78-79	22.20178734445858	27.742961539187533	27.419942989937212	22.63530812641667
80-81	22.23521045156624	27.667667665215887	27.47783268485397	22.619289198363898
82-83	22.47098878834625	27.625209861303734	27.58489103434575	22.318910316004263
84-85	22.50661351629691	27.689295467082843	27.337103967195027	22.466987049425217
86-87	22.2891177981089	27.706313393829703	27.48934031071001	22.515228497351387
88-89	22.492712792209343	27.474175835707403	27.453597550246855	22.5795138218364
90-91	22.474425527172905	27.50316569516993	27.593016663981928	22.429392113675238
92-93	22.486334754478374	27.497568997134703	27.517323483791383	22.498772764595543
94-95	22.237212341427856	27.533529369576133	27.659316227056312	22.569942061939702
96-97	22.29759654097959	27.631084503261793	27.759572407193456	22.31174654856516
98-99	23.33315727615681	29.599294401365732	27.991983351519668	19.07556497095779
100	29.935553504669244	41.04295850267714	29.021487992653615	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1306.0
1	1335.0
2	2354.5
3	3573.5
4	5116.0
5	8628.0
6	11355.5
7	12795.5
8	13225.0
9	12188.5
10	10648.0
11	9479.0
12	10510.5
13	14399.0
14	22372.5
15	32915.5
16	40885.5
17	44447.5
18	44372.5
19	41879.5
20	38213.0
21	34899.0
22	33607.5
23	35424.0
24	40775.5
25	49050.0
26	60476.5
27	72249.0
28	83990.5
29	98017.5
30	111669.5
31	124381.0
32	138480.0
33	153575.5
34	168130.5
35	183268.0
36	198036.0
37	210566.0
38	218988.0
39	223253.5
40	227789.0
41	232144.0
42	237044.0
43	245051.0
44	255096.5
45	264666.0
46	276404.5
47	288448.0
48	298482.0
49	295499.0
50	282495.0
51	273449.5
52	260490.5
53	244286.5
54	227735.5
55	211778.5
56	195593.5
57	174549.5
58	153492.0
59	132750.5
60	111130.0
61	90880.0
62	72279.0
63	56226.0
64	42178.0
65	31507.5
66	23291.5
67	17747.5
68	13640.5
69	10019.5
70	7230.5
71	5050.0
72	3442.5
73	2154.0
74	1404.0
75	935.5
76	632.5
77	397.0
78	255.0
79	171.5
80	113.5
81	77.0
82	55.5
83	36.0
84	23.0
85	16.5
86	13.0
87	8.5
88	5.5
89	5.0
90	4.0
91	3.5
92	2.5
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0013366273587529418
18-19	4.981219970507857E-5
20-21	0.0
22-23	0.006858787447754681
24-25	0.007823939110816795
26-27	0.020706851475039104
28-29	0.016667826927074015
30-31	0.002251097785353324
32-33	0.006673928995592109
34-35	0.03263818079244324
36-37	0.005766122341320814
38-39	0.002810210017221345
40-41	0.00766453113667871
42-43	0.007610303043679784
44-45	0.008628339737020404
46-47	0.005192301304590797
48-49	0.0011229990199281281
50-51	0.0026318157661045904
52-53	0.001043564210266508
54-55	0.005527642633337864
56-57	9.475492034664509E-4
58-59	0.0014557899678601736
60-61	9.404357631774216E-4
62-63	0.0017067165749445265
64-65	5.084878271192237E-4
66-67	0.0058319359186881255
68-69	0.004627724876026556
70-71	0.0018865195600636386
72-73	0.0016341730964140415
74-75	8.598359363317051E-4
76-77	8.50750379558737E-4
78-79	0.0010339564523992298
80-81	0.001077691648171396
82-83	0.0074118254802089285
84-85	0.0010544924873762639
86-87	1.9414160870137514E-4
88-89	6.599791420191955E-4
90-91	3.2378622661959896E-4
92-93	0.002926504968984569
94-95	0.003592972767671167
96-97	2.917526486034822E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	167.0
20-21	696.0
22-23	1372.0
24-25	2228.0
26-27	4418.0
28-29	10984.0
30-31	24243.0
32-33	32563.0
34-35	43285.0
36-37	64821.0
38-39	78655.0
40-41	74983.0
42-43	86461.0
44-45	94604.0
46-47	93343.0
48-49	88378.0
50-51	80420.0
52-53	74669.0
54-55	79502.0
56-57	84794.0
58-59	87817.0
60-61	89089.0
62-63	83904.0
64-65	87169.0
66-67	93989.0
68-69	86748.0
70-71	79664.0
72-73	72746.0
74-75	71742.0
76-77	71575.0
78-79	75777.0
80-81	76631.0
82-83	72098.0
84-85	71336.0
86-87	74144.0
88-89	80035.0
90-91	84311.0
92-93	86123.0
94-95	95814.0
96-97	196712.0
98-99	1091025.0
100-101	2173586.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.7609668327235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.28478637297955	73.89295243680965
2	6.944693350722734	11.494990721251995
3	1.9519606616073863	4.846384547222098
4	0.7992780032259519	2.645960812604339
5	0.3741237351155009	1.54814210166143
6	0.20975480595027182	1.041570632295288
7	0.12323845854991945	0.7139533786396097
8	0.08173151624245002	0.5411343443943686
9	0.05154849881587833	0.38395832406998337
>10	0.17250376386255487	2.2347619940544425
>50	0.004790236754154446	0.2602565302136096
>100	0.0012974830782616874	0.21848454319068605
>500	2.9311309548250223E-4	0.17744963359245497
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.320813313671905E-5	0.0	0.0	0.0	0.0
3	3.320813313671905E-5	0.0	0.0	0.0	0.0
4	3.320813313671905E-5	0.0	0.0	0.0	0.0
5	1.1622846597851666E-4	0.0	0.0	0.0	0.0
6	1.4943659911523572E-4	0.0	0.0	0.0	0.0
7	1.6604066568359524E-4	0.0	0.0	0.0	0.0
8	1.9924879882031427E-4	0.0	0.0	0.0	0.0
9	1.9924879882031427E-4	0.0	0.0	0.0	0.0
10-11	1.9924879882031427E-4	0.0	0.0	1.6604066568359524E-5	0.0
12-13	2.3245693195703333E-4	0.0	0.0	1.6604066568359524E-5	0.0
14-15	3.1547726479883096E-4	0.0	0.0	1.6604066568359524E-5	0.0
16-17	3.237792980830107E-4	0.0	0.0	1.6604066568359524E-5	0.0
18-19	4.317057307773476E-4	0.0	0.0	1.6604066568359524E-5	0.0
20-21	5.147260636191452E-4	0.0	0.0	1.6604066568359524E-5	0.0
22-23	5.396321634716845E-4	0.0	0.0	4.1510166420898804E-5	0.0
24-25	6.475585961660214E-4	0.0	0.0	1.5773863239941548E-4	0.0
26-27	6.475585961660214E-4	0.0	0.0	2.4906099852539285E-4	0.0
28-29	8.468073949863357E-4	0.0	0.0	8.385053617021559E-4	0.0
30-31	9.298277278281333E-4	0.0	0.0	0.002366079485991232	0.0
32-33	9.464317943964928E-4	0.0	0.0	0.005620476533389699	0.0
34-35	9.79639927533212E-4	0.0	0.0	0.009572244376659265	0.0
36-37	0.0010294521272382904	0.0	0.0	0.016653878768064602	0.0
38-39	0.0011622846597851666	0.0	0.0	0.02710613867284692	0.0
40-41	0.0011622846597851666	0.0	0.0	0.03847162223888902	0.0
42-43	0.0013449293920371214	0.0	0.0	0.04838424998019965	0.0
44-45	0.0015441781908574358	0.0	0.0	0.059218403416054236	0.0
46-47	0.0017600310562461093	0.0	0.0	0.07114842524542056	0.0
48-49	0.0019094676553613452	0.0	0.0	0.0824142844120525	0.0
50-51	0.0019094676553613452	0.0	0.0	0.09445223267411315	0.0
52-53	0.0019094676553613452	0.0	0.0	0.10489619054561128	0.0
54-55	0.0019343737552138846	0.0	0.0	0.11565562568190826	0.0
56-57	0.002191736787023457	0.0	0.0	0.12697959908152945	0.0
58-59	0.002191736787023457	0.0	0.0	0.13784696065052077	0.0
60-61	0.0022000388203076366	0.0	0.0	0.1480916697231986	0.0
62-63	0.0022249449201601763	0.0	0.0	0.15850241946156002	0.0
64-65	0.0022581530532968953	0.0	0.0	0.16835693296988138	0.0
66-67	0.0023909855858437715	0.0	0.0	0.1781533322452135	0.0
68-69	0.00244079778554885	0.0	0.0	0.18863880028313257	0.0
70-71	0.0024906099852539283	0.0	0.0	0.19683290713461796	0.0
72-73	0.002548724218243187	0.0	0.0	0.20408058219170688	0.0
74-75	0.002573630318095726	0.0	0.0	0.20926935299431926	0.0
76-77	0.002573630318095726	0.0	0.0	0.2142090627984062	0.0
78-79	0.0025819323513799055	0.0	0.0	0.21731402324668944	0.0
80-81	0.0027894831834843997	0.0	0.0	0.21849291197304296	0.0
82-83	0.0028060872500527594	0.0	0.0	0.21916537666906152	0.0
84-85	0.002847597416473658	0.0	0.0	0.21942273970087112	0.0
86-87	0.0029057116494629167	0.0	0.0	0.21953066613356542	0.0
88	0.002922315716031276	0.0	0.0	0.21953896816684962	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10390	0.0	39.22205	1
CTTATAC	7050	0.0	39.182476	1
TATACAC	9125	0.0	33.31258	3
GTATCAA	17070	0.0	30.511122	1
TTATACA	9465	0.0	30.299606	2
AACGCAG	20845	0.0	24.798162	6
ATCAACG	20725	0.0	24.790926	3
CAACGCA	20950	0.0	24.580624	5
TCAACGC	21020	0.0	24.517355	4
TATCAAC	21190	0.0	24.431293	2
ACACATC	11835	0.0	23.63776	6
TACACAT	12630	0.0	23.356358	5
ACATCTC	12140	0.0	23.043894	8
TACATGG	24075	0.0	22.509802	2
GTACATG	25130	0.0	22.155619	1
ACATGGG	24615	0.0	21.635033	3
ACGCAGA	23830	0.0	21.626303	7
ATACACA	14550	0.0	21.61695	4
CGCAGAG	23905	0.0	21.509418	8
CACATCT	12945	0.0	21.459969	7
>>END_MODULE