FastQCFastQC Report
Fri 27 May 2016
SRR2139876_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139876_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4154061
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC55250.045.4116481
TATACAC62400.040.652173
GGTATCA50050.039.8507731
TTATACA69100.036.3096052
GTATCAA84500.032.361721
TACACAT86650.029.5948875
ATACACA96400.027.5868764
ACACATC94150.026.564836
CGTCGAA1600.009402689526.51748794
ACATCTC100750.025.0209988
TCAACGC106900.024.914244
CAACGCA107050.024.8423615
ATCAACG107800.024.7062363
TATCAAC110800.024.2873122
CACATCT103150.024.2470077
AACGCAG113550.023.699116
TACATGG133950.022.0988252
GTACATG137150.022.0448841
ACATGGG134500.021.5376873
ACGCAGA124400.021.4730477
Produced by FastQC (version 0.11.5)