##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139876_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4154061
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.335904792924325	34.0	31.0	34.0	31.0	34.0
2	32.54444361794398	34.0	31.0	34.0	31.0	34.0
3	32.59023134229372	34.0	31.0	34.0	31.0	34.0
4	35.80189867216683	37.0	35.0	37.0	34.0	37.0
5	35.67857982826925	37.0	35.0	37.0	34.0	37.0
6	36.12277214995158	37.0	35.0	37.0	35.0	37.0
7	36.077586005597894	37.0	35.0	37.0	35.0	37.0
8	36.06000561859828	37.0	35.0	37.0	35.0	37.0
9	37.635104539870746	39.0	37.0	39.0	35.0	39.0
10-11	37.53660213463404	39.0	37.0	39.0	35.0	39.0
12-13	37.49154177081174	39.0	37.0	39.0	35.0	39.0
14-15	38.63508829071118	40.0	38.0	41.0	35.0	41.0
16-17	38.60451339063148	40.0	38.0	41.0	35.0	41.0
18-19	38.60091269723772	40.0	38.0	41.0	35.0	41.0
20-21	38.59673340514798	40.0	38.0	41.0	35.0	41.0
22-23	38.52978143636405	40.0	38.0	41.0	34.0	41.0
24-25	38.46756293216213	40.0	38.0	41.0	34.0	41.0
26-27	38.30993690121108	40.0	38.0	41.0	34.0	41.0
28-29	38.19193977717761	40.0	38.0	41.0	34.0	41.0
30-31	38.09326354193412	40.0	38.0	41.0	34.0	41.0
32-33	37.992409204937765	39.5	38.0	41.0	34.0	41.0
34-35	38.01046750345308	40.0	38.0	41.0	34.0	41.0
36-37	37.97398130396332	40.0	38.0	41.0	34.0	41.0
38-39	37.892195912451385	39.5	37.0	41.0	33.0	41.0
40-41	37.864002840420696	39.0	37.0	41.0	33.0	41.0
42-43	37.85020330716421	39.0	37.0	41.0	33.0	41.0
44-45	37.8721441619325	39.5	37.0	41.0	33.0	41.0
46-47	37.854857514054174	39.5	37.0	41.0	33.0	41.0
48-49	37.77438829279726	39.0	37.0	41.0	33.0	41.0
50-51	37.67944619300839	39.0	37.0	41.0	33.0	41.0
52-53	37.47576369262174	39.0	37.0	41.0	32.5	41.0
54-55	37.356431545171404	39.0	36.0	41.0	32.0	41.0
56-57	37.25923553607869	38.5	36.0	40.5	32.0	41.0
58-59	37.07594455001821	38.0	35.5	40.0	32.0	41.0
60-61	36.880034063413646	38.0	35.0	40.0	31.5	41.0
62-63	36.6500146636443	38.0	35.0	40.0	31.0	41.0
64-65	36.37725249698484	38.0	35.0	40.0	31.0	41.0
66-67	36.1062038020047	37.0	35.0	39.0	31.0	41.0
68-69	35.82837527366935	37.0	35.0	39.0	31.0	41.0
70-71	35.56053290414394	36.5	34.0	39.0	30.0	40.5
72-73	35.278822379571125	36.0	34.0	38.0	30.0	40.0
74-75	34.984401684429244	35.5	34.0	38.0	30.0	39.5
76-77	33.83292546051226	34.5	32.5	37.0	28.5	39.0
78-79	34.442271856121096	35.0	34.0	37.0	29.5	39.0
80-81	34.39942078885744	35.0	34.0	37.0	30.0	38.5
82-83	34.18567578981819	35.0	34.0	37.0	30.0	38.0
84-85	34.047895700638534	35.0	34.0	36.0	30.0	38.0
86-87	33.87504998436046	35.0	34.0	36.0	29.0	38.0
88-89	33.80242519633805	35.0	34.0	36.0	29.5	38.0
90-91	33.65823122942663	35.0	33.5	35.0	29.0	37.5
92-93	33.58950691121393	35.0	33.0	35.0	29.0	37.0
94-95	33.57893371849494	35.0	33.0	35.0	29.0	37.0
96-97	33.6141124247515	35.0	33.0	35.0	29.5	37.0
98-99	33.67273113642317	35.0	33.0	35.0	30.0	37.0
100	32.56417779309827	34.0	31.0	35.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	7.0
17	17.0
18	25.0
19	28.0
20	98.0
21	257.0
22	932.0
23	2444.0
24	5638.0
25	11638.0
26	20040.0
27	31295.0
28	44177.0
29	60249.0
30	78565.0
31	97062.0
32	121987.0
33	156804.0
34	212257.0
35	297961.0
36	477948.0
37	1050799.0
38	893897.0
39	563155.0
40	26779.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.808750521477656	20.03617664738192	12.857779411520436	25.297293419619983
2	15.339688078725855	20.95701050129018	40.05564675145599	23.647654668527977
3	17.83548676824919	26.579508582083893	30.715966857491985	24.869037792174932
4	12.437540036123687	17.981464403146706	37.867691398850425	31.713304161879186
5	12.923907472711644	38.06622001939788	35.86225623552471	13.147616272365765
6	30.095297108058837	37.72440992079799	18.411862512370426	13.768430458772754
7	26.579965965834397	32.213778276245826	23.042102655690417	18.16415310222936
8	25.308294702461037	35.48496278701733	21.401611579608485	17.805130930913148
9	26.183943856385355	15.62846573509633	20.580391091994073	37.607199316524245
10-11	24.58189708817468	26.180645878815934	28.91612569001755	20.321331342991833
12-13	25.336471948774946	23.60076079768689	28.582343879880433	22.48042337365773
14-15	22.56336630588718	24.802548638549123	25.76280174990208	26.871283305661613
16-17	22.45481881667223	28.03013579451309	26.563620532224746	22.95142485658993
18-19	22.548536262965932	27.151648544231076	27.963312509523817	22.336502683279175
20-21	23.18406556502505	26.46312328445262	27.619560502098977	22.733250648423343
22-23	22.932998977308774	26.85396944446618	27.448298112987214	22.76473346523783
24-25	22.905361382379066	27.08391606960156	27.593697670383456	22.417024877635917
26-27	22.89878117237774	27.22123443940916	27.534831390292265	22.345152997920835
28-29	22.520281020552382	27.19262503083111	27.636303623519836	22.650790325096676
30-31	23.401474038599055	26.986917834958703	27.335943871733328	22.27566425470892
32-33	22.30160109239855	27.643547375366005	27.48348542654145	22.571366105694
34-35	22.72365092620248	27.401074016957523	27.710929737835023	22.16434531900498
36-37	22.490102834265386	27.374018325410855	27.60288951565891	22.53298932466485
38-39	22.432407206521994	26.89818099314329	27.95677357760853	22.71263822272619
40-41	22.56350992259312	26.67982074407891	28.19411418268506	22.56255515064291
42-43	22.872063559685763	26.639380172252093	27.822203287986547	22.6663529800756
44-45	22.47347802841029	27.292555020211452	27.560270056898474	22.673696894479782
46-47	23.05490355336863	26.670177285371526	27.098588885814223	23.176330275445622
48-49	22.917601826149514	26.57369136525105	27.116080540290426	23.392626268309012
50-51	22.346461696955693	26.720448628165823	27.57782474085417	23.355264934024316
52-53	22.577915268198023	26.942328207006483	27.690117141509564	22.789639383285934
54-55	22.751982054408813	26.816704532097212	27.137682924307583	23.29363048918639
56-57	23.084809350078192	27.175947697239135	26.762986662051908	22.97625629063077
58-59	22.480107440525785	27.513050719740047	27.555405648021093	22.45143619171308
60-61	22.93201257883643	28.14055368342717	26.346270265352327	22.581163472384063
62-63	22.625443261424625	28.359233379412487	26.463577063118056	22.551746296044836
64-65	22.524485632170453	28.043033023675175	26.571848361806566	22.86063298234781
66-67	22.685088927884646	27.86605727324783	26.457050608546357	22.991803190321168
68-69	22.775239618821654	27.57660136501358	26.731683470668294	22.916475545496475
70-71	22.878517430995377	26.937179040821658	26.917915090137367	23.2663884380456
72-73	22.933314069488787	27.157367550086402	26.7163166227617	23.193001757663115
74-75	22.619945715529468	27.34699653980726	26.825876203830134	23.207181540833137
76-77	22.852343501371276	27.307518204978532	26.74168438520454	23.098453908445652
78-79	22.823754796473356	27.323473462603953	26.61936134050591	23.233410400416783
80-81	22.83121773881431	27.264199270304708	26.673259146195626	23.231323844685356
82-83	23.078609494794158	27.101647870009277	26.92845629639914	22.891286338797425
84-85	23.120693043783255	27.15688512207332	26.52419389560424	23.198227938539183
86-87	22.746143873095768	27.386494132761936	26.79890868345367	23.068453310688625
88-89	22.974685340696983	27.0798212467761	26.767682803405062	23.177810609121853
90-91	22.939388817762495	27.14440355550237	26.851458940022194	23.064748686712942
92-93	23.059317036283943	27.257182095687032	26.721490303715733	22.96201056431329
94-95	22.73857173706728	27.269795216811684	26.814433712608682	23.17719933351236
96-97	22.7664505339791	27.31695068486238	26.992905511373277	22.92369326978525
98-99	23.951561245844577	29.25553052638926	27.061467054374834	19.73144117339133
100	31.099407641438404	41.26449308699027	27.636099271571325	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	780.0
1	786.0
2	1237.5
3	1811.5
4	2505.0
5	4156.5
6	5436.0
7	6066.5
8	6216.0
9	5710.0
10	5054.5
11	4433.0
12	4797.5
13	6487.0
14	9968.5
15	14572.0
16	18019.5
17	19669.5
18	20056.5
19	19276.5
20	17842.0
21	16863.0
22	17024.5
23	18750.0
24	22325.5
25	27875.5
26	36210.0
27	44798.5
28	51339.0
29	59348.0
30	67114.0
31	73898.5
32	83761.5
33	95321.5
34	105032.5
35	115170.5
36	127798.0
37	137077.5
38	141326.5
39	142967.5
40	146599.0
41	150413.0
42	152493.0
43	158020.0
44	167154.0
45	178248.0
46	188092.0
47	196794.5
48	198971.5
49	198256.0
50	198742.0
51	195799.5
52	189024.5
53	179884.0
54	171034.5
55	161321.5
56	151842.5
57	142409.0
58	131320.5
59	115485.0
60	96449.0
61	77729.5
62	61244.0
63	48135.0
64	37317.5
65	28237.5
66	20592.0
67	15294.5
68	11769.5
69	8770.0
70	6497.0
71	4681.5
72	3203.5
73	2157.0
74	1428.5
75	922.0
76	613.0
77	432.5
78	311.5
79	222.0
80	150.5
81	85.5
82	53.5
83	31.0
84	15.5
85	8.5
86	5.5
87	3.5
88	1.5
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0015406610543273196
18-19	4.814565794772874E-5
20-21	0.0
22-23	0.005994934641368684
24-25	0.00701943480426011
26-27	0.018282684996563867
28-29	0.013982045607022073
30-31	0.001739362349762577
32-33	0.0052259920230263756
34-35	0.02939577066521459
36-37	0.004677637711925533
38-39	0.0027304960665963025
40-41	0.006331244692557773
42-43	0.007006589245762922
44-45	0.007556317777978363
46-47	0.005015541631352714
48-49	0.0010240930525527315
50-51	0.0022002209129782934
52-53	9.307069513133508E-4
54-55	0.004954109954597039
56-57	0.0012823837117539626
58-59	0.0010889511151217625
60-61	8.310781226557582E-4
62-63	0.0014098093047414113
64-65	3.0209969864044563E-4
66-67	0.004866532272347163
68-69	0.003518600200308883
70-71	0.0016799674758296679
72-73	0.0012039757883722947
74-75	7.770452243627156E-4
76-77	6.048966382869327E-4
78-79	7.688992882042989E-4
80-81	0.0011828055556376948
82-83	0.006056025674715632
84-85	8.106510176192295E-4
86-87	7.333125561442426E-5
88-89	4.294779471476969E-4
90-91	4.382526751514925E-4
92-93	0.002297298902144217
94-95	0.003545869002849843
96-97	8.20574595051566E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	91.0
20-21	324.0
22-23	663.0
24-25	1080.0
26-27	2499.0
28-29	6409.0
30-31	14998.0
32-33	19462.0
34-35	26508.0
36-37	40983.0
38-39	49203.0
40-41	47098.0
42-43	52797.0
44-45	58584.0
46-47	59620.0
48-49	56337.0
50-51	52115.0
52-53	49152.0
54-55	53663.0
56-57	57982.0
58-59	59592.0
60-61	61422.0
62-63	58341.0
64-65	61216.0
66-67	65646.0
68-69	59452.0
70-71	53321.0
72-73	49275.0
74-75	48532.0
76-77	48586.0
78-79	51544.0
80-81	51788.0
82-83	48357.0
84-85	47937.0
86-87	49015.0
88-89	52509.0
90-91	55586.0
92-93	57032.0
94-95	65238.0
96-97	136028.0
98-99	780754.0
100-101	1543322.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.50239281002315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.43272312541008	77.32214215548876
2	6.518148880077766	11.146346518772432
3	1.673229087588119	4.291952721243479
4	0.6287555901356487	2.1504042979710465
5	0.2874396827943053	1.2288390333733572
6	0.15068765059604736	0.7730492815729657
7	0.09524457546519055	0.5700547373114031
8	0.05199714699520387	0.3556704389907549
9	0.03769631953254307	0.2900812968147281
>10	0.12128351485925125	1.522458656325788
>50	0.0015501510702079198	0.08684032544832535
>100	0.0011999898715218654	0.228498328315989
>500	4.42856041994214E-5	0.033662208370923555
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	2.4072828973864372E-5	0.0
12-13	0.0	0.0	0.0	2.4072828973864372E-5	0.0
14-15	0.0	2.4072828973864372E-5	0.0	2.4072828973864372E-5	0.0
16-17	0.0	2.4072828973864372E-5	0.0	2.4072828973864372E-5	0.0
18-19	0.0	2.4072828973864372E-5	0.0	2.4072828973864372E-5	0.0
20-21	0.0	2.4072828973864372E-5	0.0	2.4072828973864372E-5	0.0
22-23	0.0	2.4072828973864372E-5	0.0	4.8145657947728744E-5	0.0
24-25	0.0	3.6109243460796556E-5	0.0	7.221848692159311E-5	0.0
26-27	0.0	4.8145657947728744E-5	0.0	1.6850980281705059E-4	0.0
28-29	0.0	7.221848692159311E-5	0.0	9.027310865199139E-4	0.0
30-31	0.0	7.221848692159311E-5	0.0	0.002852630233402928	0.0
32-33	0.0	7.221848692159311E-5	0.0	0.006102462144874618	0.0
34-35	0.0	8.425490140852529E-5	0.0	0.01079666379477817	0.0
36-37	0.0	9.629131589545749E-5	0.0	0.01878884301410114	0.0
38-39	0.0	1.0832773038238967E-4	0.0	0.02940496059157533	0.0
40-41	0.0	1.2036414486932185E-4	0.0	0.040225697215327365	0.0
42-43	0.0	1.4443697384318622E-4	0.0	0.051010324595618606	0.0
44-45	0.0	1.4443697384318622E-4	0.0	0.06326339454331556	0.0
46-47	0.0	1.4443697384318622E-4	0.0	0.07574515636626425	0.0
48-49	0.0	1.4443697384318622E-4	0.0	0.08786582575460494	0.0
50-51	0.0	1.4443697384318622E-4	0.0	0.09950503856346837	0.0
52-53	0.0	1.4443697384318622E-4	0.0	0.11131276117514885	0.0
54-55	0.0	1.4443697384318622E-4	0.0	0.12256680872043045	0.0
56-57	0.0	1.4443697384318622E-4	0.0	0.133291254028287	0.0
58-59	0.0	1.4443697384318622E-4	0.0	0.14424439121139532	0.0
60-61	0.0	1.4443697384318622E-4	0.0	0.1544994163542615	0.0
62-63	0.0	1.4443697384318622E-4	0.0	0.16446556754944136	0.0
64-65	0.0	1.4443697384318622E-4	0.0	0.17350491482912744	0.0
66-67	0.0	1.6850980281705059E-4	0.0	0.18217113325971862	0.0
68-69	0.0	1.6850980281705059E-4	0.0	0.19090957017723137	0.0
70-71	0.0	1.6850980281705059E-4	0.0	0.19916655051526688	0.0
72-73	0.0	1.6850980281705059E-4	0.0	0.20500421154142898	0.0
74-75	0.0	1.6850980281705059E-4	0.0	0.2107335448372087	0.0
76-77	0.0	1.6850980281705059E-4	0.0	0.21560829270441623	0.0
78-79	0.0	1.6850980281705059E-4	0.0	0.2187738697144794	0.0
80-81	0.0	1.6850980281705059E-4	0.0	0.21955623665613	0.0
82-83	0.0	1.6850980281705059E-4	0.0	0.22007380247906808	0.0
84-85	0.0	1.6850980281705059E-4	0.0	0.22039878567021523	0.0
86-87	0.0	1.6850980281705059E-4	0.0	0.22050711340059764	0.0
88	0.0	1.6850980281705059E-4	0.0	0.22050711340059764	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	5525	0.0	45.411648	1
TATACAC	6240	0.0	40.65217	3
GGTATCA	5005	0.0	39.850773	1
TTATACA	6910	0.0	36.309605	2
GTATCAA	8450	0.0	32.36172	1
TACACAT	8665	0.0	29.594887	5
ATACACA	9640	0.0	27.586876	4
ACACATC	9415	0.0	26.56483	6
CGTCGAA	160	0.0094026895	26.517487	94
ACATCTC	10075	0.0	25.020998	8
TCAACGC	10690	0.0	24.91424	4
CAACGCA	10705	0.0	24.842361	5
ATCAACG	10780	0.0	24.706236	3
TATCAAC	11080	0.0	24.287312	2
CACATCT	10315	0.0	24.247007	7
AACGCAG	11355	0.0	23.69911	6
TACATGG	13395	0.0	22.098825	2
GTACATG	13715	0.0	22.044884	1
ACATGGG	13450	0.0	21.537687	3
ACGCAGA	12440	0.0	21.473047	7
>>END_MODULE