FastQCFastQC Report
Fri 27 May 2016
SRR2139875_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139875_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4025935
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC48100.040.2742081
GGTATCA46300.039.619861
TATACAC60000.034.131433
GTATCAA79650.031.1213021
TTATACA68050.029.338592
ATCAACG94500.025.4777743
TACACAT82850.025.2906135
CAACGCA96050.025.0666285
AACGCAG96250.025.0556166
TCAACGC96950.024.8339314
ACACATC80600.024.377966
TATCAAC101400.024.0559882
ACATCTC84500.023.8610448
CACATCT87700.022.2691367
ATACACA96300.022.2099194
ACGCAGA109700.021.8755047
GTACATG124600.021.4171521
TACATGG121050.021.3593942
CGCAGAG114000.021.0156968
ACATGGG122950.020.321913
Produced by FastQC (version 0.11.5)