FastQCFastQC Report
Fri 27 May 2016
SRR2139874_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139874_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11236637
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA192750.032.2980961
GTATCAA297450.026.892341
AACGCAG367100.021.277816
ATCAACG371950.020.9898323
CAACGCA375750.020.735885
TCAACGC380050.020.5218734
TACATGG385950.020.5026252
TATCAAC392850.020.2720832
GTACATG403500.020.0475351
ACATGGG399750.019.490953
CGCAGAG423000.018.3826148
ACGCAGA424250.018.3284517
CATGGGG276700.017.6168634
GTGCGCT17050.017.45259
TATACAC315550.017.1602023
CTTATAC294150.017.0644931
AGAGTAC457200.015.97999910-11
TTATACA323850.015.9589462
ATGGGGG153750.015.5849245
GCAGAGT498950.015.3411579
Produced by FastQC (version 0.11.5)