##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139874_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11236637
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65923656695504	31.0	31.0	34.0	30.0	34.0
2	31.847642938007162	31.0	31.0	34.0	30.0	34.0
3	31.86740427763218	31.0	31.0	34.0	30.0	34.0
4	35.61375454239556	37.0	35.0	37.0	33.0	37.0
5	35.41671729717708	37.0	35.0	37.0	33.0	37.0
6	35.413421916183644	37.0	35.0	37.0	33.0	37.0
7	35.28770654422671	37.0	35.0	37.0	32.0	37.0
8	35.256209130899215	37.0	35.0	37.0	32.0	37.0
9	36.798818365316954	39.0	37.0	39.0	32.0	39.0
10-11	36.6350444087497	39.0	35.0	39.0	32.0	39.0
12-13	36.58649051313128	39.0	35.0	39.0	32.0	39.0
14-15	37.64938918112243	39.0	36.5	41.0	32.0	41.0
16-17	37.65310946682713	39.0	36.0	41.0	32.0	41.0
18-19	37.65020472762447	39.0	36.5	41.0	32.0	41.0
20-21	37.629045451594735	39.0	37.0	41.0	32.0	41.0
22-23	37.53426203854254	39.0	36.0	41.0	32.0	41.0
24-25	37.415853705510045	39.0	36.0	41.0	32.0	41.0
26-27	37.219019629444055	39.0	36.0	40.5	31.0	41.0
28-29	37.05361280484981	39.0	36.0	40.0	31.0	41.0
30-31	36.90341738649116	39.0	36.0	40.0	30.5	41.0
32-33	37.07297948769818	39.0	36.0	40.0	31.0	41.0
34-35	37.03607281532287	39.0	36.0	40.0	31.0	41.0
36-37	36.91442724199681	39.0	35.0	40.0	30.5	41.0
38-39	36.71381401534293	39.0	35.0	40.0	30.0	41.0
40-41	36.70070811880046	38.5	35.0	40.0	30.0	41.0
42-43	36.581002412015124	38.0	35.0	40.0	30.0	41.0
44-45	36.62680177321657	38.5	35.0	40.0	30.0	41.0
46-47	36.77336970400577	39.0	35.0	40.0	30.0	41.0
48-49	36.70367179017811	39.0	35.0	40.0	30.0	41.0
50-51	36.5443399291203	39.0	35.0	40.0	30.0	41.0
52-53	36.407222635421704	38.0	35.0	40.0	30.0	41.0
54-55	36.262841883568015	38.0	34.5	40.0	29.5	41.0
56-57	36.059881032866045	38.0	34.0	40.0	29.0	41.0
58-59	35.9013613410325	38.0	34.0	40.0	29.0	41.0
60-61	35.683852853261584	37.5	34.0	40.0	28.0	41.0
62-63	35.42722679778988	37.0	34.0	40.0	28.0	41.0
64-65	35.200304475431295	36.5	33.5	39.5	28.0	41.0
66-67	34.92809067426275	36.0	33.0	39.0	28.0	40.5
68-69	34.606114929151246	36.0	33.0	39.0	28.0	40.0
70-71	34.26573932528283	35.0	32.0	38.5	27.0	40.0
72-73	33.97743948610728	35.0	32.0	38.0	27.0	40.0
74-75	33.62644578300893	35.0	32.0	37.0	26.0	39.0
76-77	32.57055425221663	34.0	30.5	36.0	26.0	39.0
78-79	32.94210961940156	34.0	31.0	36.0	26.0	39.0
80-81	32.84179906897185	34.0	31.0	36.0	26.0	37.5
82-83	32.41023002284882	34.0	31.0	35.0	26.0	37.0
84-85	32.171342550108406	34.0	31.0	35.0	26.0	36.5
86-87	32.10621296594489	34.0	31.0	35.0	26.0	36.0
88-89	31.96248251973398	34.0	31.0	35.0	26.0	36.0
90-91	31.847852754997454	34.0	31.0	35.0	25.5	35.5
92-93	31.77881491418642	34.0	31.0	35.0	25.0	35.0
94-95	31.745002338476695	34.0	31.0	35.0	25.0	35.0
96-97	31.67376949098439	34.0	30.5	35.0	25.0	35.0
98-99	31.920983889738082	34.0	31.0	35.0	26.0	35.0
100-101	31.03510463438319	32.5	29.0	34.5	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	8.0
19	31.0
20	136.0
21	902.0
22	3934.0
23	13056.0
24	32009.0
25	65061.0
26	112685.0
27	172015.0
28	237776.0
29	309617.0
30	382086.0
31	464994.0
32	560539.0
33	689421.0
34	873528.0
35	1129276.0
36	1553420.0
37	2014927.0
38	1744807.0
39	854250.0
40	22158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.95050956972268	22.324384066157872	14.506715843895288	25.218390520224155
2	14.552454786616753	22.785154731510605	39.41081810557313	23.251572376299514
3	16.931413802389578	27.588751222118653	31.855811980705255	23.624022994786518
4	12.043472089286517	19.31572930864089	38.1400481549859	30.500750447086695
5	12.228142637338912	38.75752148974822	36.25037455601707	12.763961316895795
6	29.401163355192484	38.82182898673331	18.46297962637754	13.314028031696672
7	25.461950937811732	33.009912129403126	23.591275574711542	17.936861358073592
8	25.02539683358998	36.18469654221276	21.402364426295875	17.387542197901382
9	25.668071327746905	15.228257351376573	20.871075571810323	38.2325957490662
10-11	24.093013772715093	26.70122742240405	29.056620766515817	20.149138038365038
12-13	24.8603118530927	24.230804109806165	29.00306826677768	21.905815770323454
14-15	22.134545238045867	25.484826999394926	26.35797970513776	26.02264805742145
16-17	21.729567247309888	28.513171234165018	27.245304811103804	22.51195670742129
18-19	21.577761661603837	27.730268289798154	28.877817399754317	21.81415264884369
20-21	22.451302621120103	27.078435841819104	28.460909084193524	22.00935245286727
22-23	22.233001873881904	27.4344830146375	28.225711721480945	22.10680338999965
24-25	22.10893175605719	27.6042218833017	28.502561146507393	21.78428521413372
26-27	22.05938096958276	27.76834040275097	28.303287194549387	21.868991433116882
28-29	21.68997977501195	27.701018705126305	28.388006558896382	22.220994960965363
30-31	22.396105956125155	27.63756008417337	28.303788833867976	21.662545125833503
32-33	22.148985348008686	27.77078526981185	28.3037065068223	21.776522875357166
34-35	22.171786037893064	27.89655842868687	28.486311185195884	21.445344348224182
36-37	22.118845925314144	27.68695960236396	28.289740336197987	21.90445413612391
38-39	21.750171861692795	27.4899315152141	28.653669192517984	22.10622743057512
40-41	21.77575927412072	27.318883958745687	28.806001761445206	22.099355005688388
42-43	22.09693178500828	27.419926688614094	28.365316038823714	22.11782548755391
44-45	21.77043453768662	27.956271650728876	28.080043404784245	22.19325040680026
46-47	22.23487906667184	27.490549431866867	27.54740797130268	22.727163530158613
48-49	22.107625889608034	27.665734755190346	27.631697578249426	22.594941776952197
50-51	21.938323156003293	27.70912707630309	27.613678367722162	22.738871399971455
52-53	21.937865121227517	27.792942671304722	27.71800057104078	22.551191636426985
54-55	21.931746148241118	27.807671301711228	27.451968301716494	22.80861424833116
56-57	22.347824696441073	27.80953913884398	27.118353574090293	22.72428259062465
58-59	21.90354015259182	27.930994310131048	27.73729936633793	22.4281661709392
60-61	22.204846993813433	28.237638617423567	27.15519820156614	22.402316187196863
62-63	22.027649147790505	28.255854791126705	27.10741153701079	22.609084524071996
64-65	21.961332402371394	28.158105552176092	27.152357915765297	22.728204129687214
66-67	22.079748607504794	28.118749573323953	26.966576146642097	22.83492567252916
68-69	22.13938308098642	28.025825325471367	27.026498892737173	22.808292700805037
70-71	22.267679533804934	27.569942386109087	27.0872613760853	23.075116704000678
72-73	22.128991493652407	27.692626918845033	27.177583643468235	23.00079794403433
74-75	22.25237134268297	27.68309914328259	27.07886086725671	22.98566864677773
76-77	22.393800081654916	27.730429312014376	26.929180112732155	22.946590493598553
78-79	22.262499835883816	27.658041933406917	26.998574366599282	23.08088386410998
80-81	22.305812629772028	27.693781702747355	26.951204054333903	23.04920161314671
82-83	22.48375520099151	27.512621980701002	27.129842625317508	22.873780192989983
84-85	22.631941931667455	27.630886797638954	26.941997177760395	22.795174092933195
86-87	22.37482488936719	27.70218453859642	27.150886668184356	22.77210390385204
88-89	22.489309087775506	27.476767384992502	27.11144554674841	22.922477980483585
90-91	22.467224424705115	27.497641596340312	27.2561986323331	22.778935346621477
92-93	22.554372528167683	27.499352467100348	27.222609980972496	22.723665023759477
94-95	22.288771564995304	27.43450601144749	27.53541353307558	22.741308890481623
96-97	22.142335260084252	27.520852700636507	27.583991980548696	22.752820058730546
98-99	22.114944479566006	28.10239629998243	27.819665211065235	21.962994009386332
100-101	24.91835036222871	35.79635345169107	29.10175264032332	10.183543545756903
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2198.0
1	2438.0
2	4730.5
3	7057.5
4	9486.5
5	14473.5
6	18050.0
7	20506.5
8	21633.0
9	20238.0
10	17993.5
11	16369.5
12	18725.0
13	27153.0
14	45463.0
15	68403.0
16	83089.5
17	86123.0
18	82585.5
19	76588.0
20	69273.5
21	63142.0
22	59945.0
23	62937.5
24	72942.0
25	88410.0
26	107413.5
27	126253.0
28	146948.0
29	172695.0
30	198062.5
31	221637.0
32	247235.0
33	272901.0
34	299260.5
35	327773.0
36	354225.0
37	380459.0
38	400928.0
39	413255.5
40	422348.0
41	427709.5
42	440262.5
43	464071.5
44	486378.5
45	508971.5
46	529496.0
47	543143.0
48	566197.5
49	569885.5
50	547222.5
51	536269.0
52	522652.5
53	493038.0
54	455984.5
55	413072.0
56	372452.5
57	326566.0
58	279501.0
59	237382.5
60	194196.0
61	155694.5
62	122631.0
63	95072.0
64	71117.0
65	51892.5
66	37988.0
67	28771.0
68	22181.5
69	16526.0
70	12271.5
71	8625.5
72	6009.0
73	4185.5
74	2818.5
75	1892.0
76	1286.5
77	826.5
78	546.5
79	394.0
80	286.5
81	198.0
82	138.5
83	95.0
84	68.0
85	52.5
86	41.5
87	29.5
88	23.0
89	17.5
90	10.0
91	6.5
92	5.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0012548238409766197
3	2.6698379595247228E-5
4	1.779891973016482E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.001437262768210809
18-19	0.0014951092573338447
20-21	2.6700435003487077E-5
22-23	0.0027284692397363045
24-25	8.014638291581891E-5
26-27	0.0011761927886907281
28-29	4.4145537044504453E-4
30-31	1.4747165214994452E-4
32-33	9.377430588505559E-4
34-35	0.016710500903129488
36-37	0.033096266206393006
38-39	0.007313217997599517
40-41	0.004375491660964563
42-43	0.0074685518096126585
44-45	0.005323901529155584
46-47	0.0030830655669815935
48-49	0.0013395224212080308
50-51	1.6566571109345794E-4
52-53	9.675195538247931E-5
54-55	0.0046654842622997185
56-57	0.012303635910512568
58-59	0.010198762308487984
60-61	0.008267690781850492
62-63	0.0019741338695014564
64-65	0.0011831257201221459
66-67	0.0013630442471768594
68-69	0.0
70-71	0.0
72-73	6.11541350836136E-4
74-75	8.608019605326044E-4
76-77	7.637242682996449E-5
78-79	8.839446915806478E-4
80-81	0.002646743999472247
82-83	0.005018577247650172
84-85	0.013100344176701903
86-87	0.008555871015142321
88-89	0.07894474987759609
90-91	0.0
92-93	0.0
94-95	1.211373342033686E-4
96-97	0.007999827122379982
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	520.0
20-21	1957.0
22-23	3567.0
24-25	5797.0
26-27	9072.0
28-29	17482.0
30-31	41427.0
32-33	56300.0
34-35	77974.0
36-37	117202.0
38-39	131305.0
40-41	134752.0
42-43	145571.0
44-45	168239.0
46-47	168689.0
48-49	159141.0
50-51	146885.0
52-53	126822.0
54-55	145968.0
56-57	158320.0
58-59	165929.0
60-61	172742.0
62-63	163849.0
64-65	173013.0
66-67	188794.0
68-69	170585.0
70-71	168536.0
72-73	160707.0
74-75	159779.0
76-77	160297.0
78-79	172313.0
80-81	177564.0
82-83	168971.0
84-85	166405.0
86-87	175128.0
88-89	189086.0
90-91	207799.0
92-93	222893.0
94-95	280002.0
96-97	330620.0
98-99	860138.0
100-101	4784497.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.774173796315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.53008741772912	73.21248928496655
2	6.412003234591963	10.486725337761143
3	1.8930562576703933	4.644093342628212
4	0.8098411161644602	2.6489635272253715
5	0.44751683070498083	1.829765954542259
6	0.26789735668681763	1.3144251003168728
7	0.16860447933430012	0.965124439714418
8	0.11955057145321378	0.7820919365971102
9	0.07663714745061409	0.5640245473392301
>10	0.27176025643838986	3.3305864291366665
>50	0.0023849796222683035	0.1285074528581783
>100	6.603521535404048E-4	0.09320264691404966
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.89945986508241E-6	0.0	0.0	0.0	0.0
2	1.4239135784131855E-4	0.0	0.0	0.0	0.0
3	1.4239135784131855E-4	0.0	0.0	0.0	0.0
4	1.4239135784131855E-4	0.0	0.0	0.0	0.0
5	2.4028541635722503E-4	0.0	0.0	0.0	0.0
6	3.4707893473821393E-4	0.0	0.0	0.0	0.0
7	4.983697524446149E-4	0.0	0.0	0.0	0.0
8	5.161686721747797E-4	0.0	0.0	0.0	0.0
9	5.161686721747797E-4	0.0	0.0	0.0	0.0
10-11	5.784648912303565E-4	0.0	0.0	8.89945986508241E-6	0.0
12-13	7.253059790042163E-4	0.0	0.0	8.89945986508241E-6	0.0
14-15	8.810465266431585E-4	0.0	0.0	8.89945986508241E-6	0.0
16-17	9.38893015766194E-4	0.0	0.0	3.114810952778843E-5	0.0
18-19	0.0012325751913139136	0.0	0.0	1.2014270817861252E-4	0.0
20-21	0.0014951092573338447	0.0	0.0	3.3372974494059034E-4	0.0
22-23	0.0016330508852426219	0.0	0.0	8.232000375201228E-4	0.0
24-25	0.002095822798226907	0.0	0.0	0.0022159655064055194	0.0
26-27	0.0021047222580919895	0.0	0.0	0.006207373255894979	0.0
28-29	0.0025719439010088163	0.0	0.0	0.03509057024801994	0.0
30-31	0.002825578507163665	0.0	0.0	0.060222644907012665	0.0
32-33	0.0028745255364216182	0.0	0.0	0.08308980703034191	0.0
34-35	0.0031726574419018785	0.0	0.0	0.11370394896622539	0.0
36-37	0.0035597839460329633	0.0	0.0	0.15765838123986742	0.0
38-39	0.003991407749489461	0.0	0.0	0.23242274356642473	0.0
40-41	0.00402700558894979	0.0	0.0	0.348222515330877	0.0
42-43	0.0043651850638229215	0.0	0.0	0.40679876016284944	0.0
44-45	0.004850205626469913	0.0	0.0	0.4927764419194106	0.0
46-47	0.005566612145609046	0.0	0.0	0.5820647227457825	0.0
48-49	0.005811347291898813	0.0	0.0	0.6628495696710679	0.0
50-51	0.005811347291898813	0.0	0.0	0.7464599951035171	0.0
52-53	0.005815797021831354	0.0	0.0	0.8352944034767698	0.0
54-55	0.005904791620482177	0.0	0.0	1.017666584761971	0.0
56-57	0.006238521365422769	0.0	0.0	1.182235396587075	0.0
58-59	0.006238521365422769	0.0	0.0	1.320964626693912	0.0
60-61	0.006323066234141051	0.0	0.0	1.401629330911019	0.0
62-63	0.006425410022589499	0.0	0.0	1.4473992529971378	0.0
64-65	0.00650550516137524	0.0	0.0	1.4786497063133748	0.0
66-67	0.006861483555978537	0.0	0.0	1.4920923404395818	0.0
68-69	0.007039472753280185	0.0	0.0	1.5044180923527208	0.0
70-71	0.007204112760784209	0.0	0.0	1.5155468669140064	0.0
72-73	0.007328705198895363	0.0	0.0	1.524481924618549	0.0
74-75	0.007377652228153317	0.0	0.0	1.532669427694425	0.0
76-77	0.0073954511478834814	0.0	0.0	1.5401494237110267	0.0
78-79	0.007413250067613646	0.0	0.0	1.5469575105078146	0.0
80-81	0.00808960901735991	0.0	0.0	1.5512959971920424	0.0
82-83	0.008205301995605981	0.0	0.0	1.5541838719182617	0.0
84-85	0.008338793893582216	0.0	0.0	1.557089545564211	0.0
86-87	0.008458936601760829	0.0	0.0	1.5575523174771955	0.0
88-89	0.008476735521490995	0.0	0.0	1.557681359645239	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19275	0.0	32.298096	1
GTATCAA	29745	0.0	26.89234	1
AACGCAG	36710	0.0	21.27781	6
ATCAACG	37195	0.0	20.989832	3
CAACGCA	37575	0.0	20.73588	5
TCAACGC	38005	0.0	20.521873	4
TACATGG	38595	0.0	20.502625	2
TATCAAC	39285	0.0	20.272083	2
GTACATG	40350	0.0	20.047535	1
ACATGGG	39975	0.0	19.49095	3
CGCAGAG	42300	0.0	18.382614	8
ACGCAGA	42425	0.0	18.328451	7
CATGGGG	27670	0.0	17.616863	4
GTGCGCT	1705	0.0	17.4525	9
TATACAC	31555	0.0	17.160202	3
CTTATAC	29415	0.0	17.064493	1
AGAGTAC	45720	0.0	15.979999	10-11
TTATACA	32385	0.0	15.958946	2
ATGGGGG	15375	0.0	15.584924	5
GCAGAGT	49895	0.0	15.341157	9
>>END_MODULE