FastQCFastQC Report
Fri 27 May 2016
SRR2139873_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139873_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7457554
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104200.033.0559431
GTATCAA160850.027.3732241
CTTATAC140700.022.2474381
TATACAC152800.022.0991213
TACATGG224300.021.6691232
TCAACGC200200.021.6348764
ATCAACG200100.021.5463053
GTACATG230950.021.4929491
CAACGCA201450.021.3821725
AACGCAG201700.021.2767946
ACATGGG225350.021.1617833
TATCAAC209900.020.8060322
ACGCAGA225650.019.0361487
TTATACA172600.018.8269792
CATGGGG154950.018.6866054
CGCAGAG233250.018.398848
TACACAT183500.018.1417855
ACACATC181800.017.1956796
ATACACA207000.016.869994
CACATCT197600.015.9012337
Produced by FastQC (version 0.11.5)