FastQCFastQC Report
Fri 27 May 2016
SRR2139872_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139872_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7007173
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC89150.036.170091
GGTATCA119600.035.3094671
TATACAC111900.031.3854273
GTATCAA191900.028.7883911
TTATACA120150.028.0341972
TACACAT149400.023.5855715
ACACATC142750.022.9425226
ATCAACG245200.022.1026043
ATACACA170100.022.0857334
ACATCTC146450.022.0710858
AACGCAG246350.022.0151966
CAACGCA246750.021.9322745
TATCAAC251400.021.6812932
TCAACGC250500.021.6194554
CACATCT156300.020.6553127
TACATGG253450.020.5708872
CATCTCC161150.020.1300989
GTACATG264750.020.1037651
ACATGGG257350.019.6551573
ACGCAGA278800.019.3692027
Produced by FastQC (version 0.11.5)