##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139872_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7007173
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.722772364832437	31.0	31.0	34.0	30.0	34.0
2	31.90242841157197	31.0	31.0	34.0	30.0	34.0
3	31.92566774075651	31.0	31.0	34.0	30.0	34.0
4	35.66136300616525	37.0	35.0	37.0	33.0	37.0
5	35.472464715799084	37.0	35.0	37.0	33.0	37.0
6	35.470171351556466	37.0	35.0	37.0	33.0	37.0
7	35.34818906283604	37.0	35.0	37.0	32.0	37.0
8	35.317627665251024	37.0	35.0	37.0	32.0	37.0
9	36.86097132181552	39.0	37.0	39.0	33.0	39.0
10-11	36.707571156013984	39.0	35.0	39.0	32.0	39.0
12-13	36.66587638124533	39.0	35.0	39.0	32.0	39.0
14-15	37.75554742261965	40.0	37.0	41.0	32.0	41.0
16-17	37.76932380576304	40.0	37.0	41.0	32.0	41.0
18-19	37.77011592264098	40.0	37.0	41.0	32.0	41.0
20-21	37.7490015532905	40.0	37.0	41.0	32.0	41.0
22-23	37.65944359132162	39.0	37.0	41.0	32.0	41.0
24-25	37.54148030418668	39.0	36.0	41.0	32.0	41.0
26-27	37.36103225361215	39.0	36.0	41.0	31.5	41.0
28-29	37.217378275918406	39.0	36.0	40.5	31.0	41.0
30-31	37.06566113277073	39.0	36.0	40.0	31.0	41.0
32-33	37.21607706249715	39.0	36.0	40.0	31.0	41.0
34-35	37.18531674706874	39.0	36.0	41.0	31.0	41.0
36-37	37.07296423938921	39.0	35.5	40.0	31.0	41.0
38-39	36.88375441556001	39.0	35.0	40.0	30.0	41.0
40-41	36.88379202592834	39.0	35.0	40.0	30.0	41.0
42-43	36.76729733339505	39.0	35.0	40.0	30.0	41.0
44-45	36.82454048580423	38.5	35.0	40.0	30.5	41.0
46-47	36.973859236417184	39.0	35.0	40.0	31.0	41.0
48-49	36.91207419412081	39.0	35.0	40.0	30.5	41.0
50-51	36.782046066397086	39.0	35.0	40.0	30.0	41.0
52-53	36.66286710390453	39.0	35.0	40.0	30.0	41.0
54-55	36.52817065378491	38.0	35.0	40.0	30.0	41.0
56-57	36.35353570500951	38.0	35.0	40.0	29.5	41.0
58-59	36.21411023181365	38.0	34.5	40.0	29.0	41.0
60-61	35.997069035375134	38.0	34.0	40.0	29.0	41.0
62-63	35.75173870680469	37.5	34.0	40.0	29.0	41.0
64-65	35.540874137125506	37.0	34.0	40.0	28.5	41.0
66-67	35.27137887777992	36.5	34.0	39.0	28.0	41.0
68-69	34.951765815240826	36.0	33.0	39.0	28.0	40.5
70-71	34.614117325043935	36.0	33.0	39.0	28.0	40.0
72-73	34.32966999564887	35.0	33.0	38.0	28.0	40.0
74-75	33.977612034447596	35.0	32.0	37.5	27.5	39.5
76-77	32.90436605167943	34.0	31.0	36.0	26.0	39.0
78-79	33.26130226387944	35.0	32.0	36.0	26.5	39.0
80-81	33.150812775812895	35.0	32.0	36.0	27.0	38.5
82-83	32.716452944723414	34.0	31.5	35.5	26.0	37.0
84-85	32.47407973375779	34.0	31.0	35.0	26.0	37.0
86-87	32.38569792023442	34.0	31.0	35.0	26.0	36.0
88-89	32.22359325387077	34.0	31.0	35.0	26.0	36.0
90-91	32.09025002972367	34.0	31.0	35.0	26.0	36.0
92-93	32.00386379195025	34.0	31.0	35.0	26.0	35.0
94-95	31.9623438236017	34.0	31.0	35.0	26.0	35.0
96-97	31.878643869165373	34.0	31.0	35.0	26.0	35.0
98-99	32.095681355013625	34.0	31.0	35.0	26.5	35.0
100-101	31.212241177404593	33.0	29.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	2.0
19	22.0
20	100.0
21	580.0
22	2295.0
23	7286.0
24	17779.0
25	36265.0
26	62654.0
27	96515.0
28	135428.0
29	177018.0
30	221777.0
31	272375.0
32	331157.0
33	408811.0
34	522003.0
35	674033.0
36	935925.0
37	1266846.0
38	1195573.0
39	623773.0
40	18954.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.193762306139725	22.36565302440799	14.421379349418089	25.0192053200342
2	14.32209796195755	22.73556111280226	40.08169181199494	22.860649113245245
3	16.737408472348044	27.870558907359488	32.14433725902547	23.247695361267
4	11.653460197637507	19.45559492474282	38.97167073963648	29.919274137983198
5	11.868566681598985	39.23846321476579	36.514211936825305	12.378758166809925
6	28.73963579891634	39.45975074398763	18.769281135202455	13.03133232189358
7	25.06879735950575	33.489739728132875	23.82699842004757	17.614464492313804
8	24.776425528526268	36.487353744512944	21.615664405602658	17.12055632135813
9	25.673078144352935	15.576281618849713	21.208067789963227	37.54257244683412
10-11	23.762321837922368	26.98688900645096	29.602765623169287	19.648023532457383
12-13	24.59673822809855	24.614334482679393	29.330144981435453	21.458782307786606
14-15	21.793617768535185	25.89660623478256	26.83042790580452	25.479348090877735
16-17	21.347190137477234	28.825029245541383	27.717209422337586	22.110571194643796
18-19	21.206690983529995	28.0183383314984	29.437861451820492	21.33710923315111
20-21	22.071670586757705	27.298676683831335	29.06318149640912	21.566471233001838
22-23	21.710860005296205	27.809219537716906	28.749046219030223	21.730874237956662
24-25	21.519522026874743	27.96212611761218	29.159722143384943	21.358629712128135
26-27	21.62578916923076	28.0647442577671	28.99259030540727	21.31687626759487
28-29	21.397829799659345	27.926509745705776	29.16186236660983	21.51379808802505
30-31	21.793362295720268	27.9347067529651	28.889959463744734	21.381971487569896
32-33	21.551088625306893	27.903637820201375	28.934681042034207	21.610592512457526
34-35	21.392760124390477	28.350320372951355	29.26661278613415	20.990306716524014
36-37	21.753440133655285	28.113322874298202	28.775503753214853	21.35773323883166
38-39	21.325957318704795	27.978669323178202	29.014471000943086	21.680902357173913
40-41	21.48454456847702	27.808147986014063	29.108136574268944	21.59917087123997
42-43	21.772933578139206	27.85593975985409	28.760937660596962	21.610189001409747
44-45	21.425083433728048	28.458968662597574	28.516721141343666	21.599226762330716
46-47	21.876945923038864	28.043613765785942	27.843546807608142	22.235893503567052
48-49	21.829489087014668	28.057765697227214	27.896269135830636	22.216476079927475
50-51	21.61261824410946	28.06177027489301	28.07110939312446	22.25450208787307
52-53	21.66596095749873	28.1545817886159	28.251883946800355	21.92757330708502
54-55	21.655097213795337	28.209335360865538	27.863922035732536	22.27164538960659
56-57	22.023315927079828	28.3782770501598	27.458305257356585	22.140101765403784
58-59	21.64443312876864	28.623142769617367	28.1235034061467	21.608920695467297
60-61	22.007107241249095	28.91086198431661	27.433579346411825	21.64845142802247
62-63	21.818639283466837	28.960661696441537	27.369900058449154	21.85079896164247
64-65	21.719671502203603	28.75586364697771	27.51102262003291	22.013442230785778
66-67	21.85639460547162	28.570910847917215	27.459057248077386	22.113637298533778
68-69	21.916644702484454	28.30504369101343	27.62291520165331	22.15539640484881
70-71	21.937097318297592	28.009121842595718	27.69661209169098	22.357168747415702
72-73	21.9685613831646	28.11346476069168	27.71013117976499	22.207842676378732
74-75	21.91585633552465	28.149411740211217	27.68847317813786	22.246258746126273
76-77	21.948527352703522	28.121272525164915	27.640522613182505	22.28967750894906
78-79	21.839127367145423	28.106125312752635	27.6426621215179	22.41208519858404
80-81	21.832707725143404	27.93493645839606	27.890730484426605	22.341625332033928
82-83	22.033370496712426	27.919262048995357	27.919351054194262	22.12801640009795
84-85	22.200410721730567	27.987848116706143	27.59554832989548	22.21619283166781
86-87	21.873702525684024	28.080679936503365	27.783234495334742	22.262383042477868
88-89	22.027001762646588	27.914755042298147	27.66712440127656	22.391118793778713
90-91	22.01891540994988	27.79176232411417	27.907109770089477	22.282212495846473
92-93	22.028485995366925	27.76850839467608	27.9342936715304	22.26871193842659
94-95	21.625623311669663	27.825138883991773	28.178884599963116	22.370353204375448
96-97	21.601894822228786	27.810127821522094	28.198438038605605	22.389539317643518
98-99	21.618440880146224	28.537006680570126	28.277048929744204	21.567503509539446
100-101	24.232163584348893	36.31611557369296	29.639920080556532	9.811800761401607
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1525.0
1	1657.0
2	3127.5
3	4727.5
4	6541.5
5	10135.5
6	12699.0
7	14379.5
8	15083.5
9	14104.0
10	12520.0
11	11368.5
12	13021.5
13	18561.0
14	30480.5
15	45118.5
16	54498.0
17	56902.5
18	55073.5
19	51632.0
20	47594.0
21	44685.5
22	44002.0
23	46878.5
24	54192.0
25	65772.5
26	80323.0
27	94936.5
28	109878.5
29	126879.0
30	142908.5
31	158843.5
32	176987.0
33	194696.0
34	211621.0
35	227453.0
36	241523.5
37	256977.5
38	267707.0
39	273407.5
40	280971.5
41	285428.5
42	288120.5
43	295478.5
44	303446.5
45	312240.5
46	327977.0
47	343719.0
48	341761.5
49	329465.5
50	317851.0
51	304944.5
52	289944.5
53	270723.0
54	252073.0
55	233103.0
56	213916.0
57	191084.5
58	166936.5
59	141401.5
60	113624.5
61	90220.5
62	70561.5
63	53940.5
64	40400.5
65	30003.0
66	21804.0
67	16426.0
68	12608.5
69	9319.5
70	6959.5
71	4882.0
72	3393.0
73	2299.0
74	1519.5
75	1089.5
76	752.5
77	450.0
78	287.5
79	215.5
80	166.5
81	124.5
82	100.5
83	71.5
84	43.5
85	30.0
86	22.0
87	18.0
88	11.5
89	6.0
90	5.0
91	4.0
92	2.0
93	1.5
94	2.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0015127355925135572
3	1.4271090495410916E-5
4	1.4271090495410916E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.001741073040440132
18-19	0.001448515685284208
20-21	7.136087544380221E-6
22-23	0.0025338347233141703
24-25	1.3565944054046722E-4
26-27	0.0013145057062049742
28-29	5.363576083542488E-4
30-31	1.863666488567051E-4
32-33	9.141072443574931E-4
34-35	0.01664063927849547
36-37	0.03237257004125897
38-39	0.00773968142791805
40-41	0.004744067651901267
42-43	0.007417962680062891
44-45	0.005315071671430588
46-47	0.003112595923032481
48-49	0.0013403348810743997
50-51	1.2170860380027814E-4
52-53	1.3185673567883393E-4
54-55	0.004956117846101323
56-57	0.012489906264361049
58-59	0.01042434279335932
60-61	0.00863206933278088
62-63	0.0019416446380309304
64-65	0.0014461726258497185
66-67	0.001675513471928419
68-69	0.0
70-71	0.0
72-73	7.60793202993441E-4
74-75	8.981296450142578E-4
76-77	9.369102345263288E-5
78-79	9.9897052898784E-4
80-81	0.0027064149534099906
82-83	0.005251030987646616
84-85	0.01299266608370326
86-87	0.00851035789722212
88-89	0.07220975950294282
90-91	0.0
92-93	1.2662129066094541E-5
94-95	2.621496877338457E-4
96-97	0.007842373771372868
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	329.0
20-21	1177.0
22-23	2167.0
24-25	3515.0
26-27	5855.0
28-29	12392.0
30-31	26905.0
32-33	35904.0
34-35	50207.0
36-37	75798.0
38-39	89430.0
40-41	88429.0
42-43	97257.0
44-45	111578.0
46-47	112239.0
48-49	106711.0
50-51	97128.0
52-53	92947.0
54-55	101512.0
56-57	107653.0
58-59	111299.0
60-61	113846.0
62-63	107816.0
64-65	111372.0
66-67	118081.0
68-69	104105.0
70-71	102674.0
72-73	96018.0
74-75	96049.0
76-77	96359.0
78-79	103596.0
80-81	106943.0
82-83	100490.0
84-85	98845.0
86-87	104438.0
88-89	112993.0
90-91	122905.0
92-93	129363.0
94-95	160272.0
96-97	191893.0
98-99	520187.0
100-101	2978496.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.02196124167116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.82986701667043	74.57851737802487
2	6.404557090619001	10.634377810948818
3	1.8284693490250852	4.55409334279033
4	0.7922178732112088	2.6308592625880056
5	0.42149463649564045	1.7496655687356668
6	0.2250069787343168	1.1208312400551586
7	0.15022288996862557	0.8730259264010944
8	0.091190734842526	0.6056666922956564
9	0.0597032630951629	0.44610137952191203
>10	0.1921255549837539	2.4623136040623907
>50	0.004576934534812427	0.24482796710339622
>100	5.368133441953584E-4	0.08319215867086469
>500	3.086447529392277E-5	0.016527668801797898
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	5.7084361981643666E-5	1.4271090495410916E-5	0.0	1.4271090495410916E-5	0.0
3	5.7084361981643666E-5	1.4271090495410916E-5	0.0	2.8542180990821833E-5	0.0
4	5.7084361981643666E-5	4.281327148623275E-5	0.0	7.135545247705459E-5	0.0
5	1.71253085944931E-4	4.281327148623275E-5	0.0	7.135545247705459E-5	0.0
6	2.4260853842198558E-4	4.281327148623275E-5	0.0	7.135545247705459E-5	0.0
7	4.281327148623275E-4	4.281327148623275E-5	0.0	8.56265429724655E-5	0.0
8	4.566748958531493E-4	4.281327148623275E-5	0.0	9.989763346787642E-5	0.0
9	4.566748958531493E-4	4.281327148623275E-5	0.0	2.7115071941280743E-4	0.0
10-11	4.566748958531493E-4	4.281327148623275E-5	0.0	2.925573551559238E-4	0.0
12-13	5.13759257834793E-4	4.281327148623275E-5	0.0	3.3537062664215655E-4	0.0
14-15	6.350635270457859E-4	4.281327148623275E-5	0.0	4.2099716961462204E-4	0.0
16-17	7.420967057613677E-4	4.281327148623275E-5	0.0	4.780815315962657E-4	0.0
18-19	9.775696989356478E-4	7.135545247705459E-5	0.0	4.8521707684397115E-4	0.0
20-21	0.0011416872396328734	7.135545247705459E-5	0.0	5.28030348330204E-4	0.0
22-23	0.0011773649658714006	7.135545247705459E-5	0.0	5.423014388256149E-4	0.0
24-25	0.001448515685284208	8.56265429724655E-5	0.0	5.92250255559553E-4	0.0
26-27	0.001462786775779619	8.56265429724655E-5	0.0	6.636057080366077E-4	0.0
28-29	0.001819564038164892	9.276208822017096E-5	0.0	0.0013985668685502699	0.0
30-31	0.0019694104883667064	9.989763346787642E-5	0.0	0.0032252664519628674	0.0
32-33	0.0019979526693575285	9.989763346787642E-5	0.0	0.006186517729760633	0.0
34-35	0.002240561207779514	1.1416872396328733E-4	0.0	0.010061118799264697	0.0
36-37	0.0024831697462014994	1.1416872396328733E-4	0.0	0.01659014270091519	0.0
38-39	0.002754320465614307	1.1416872396328733E-4	0.0	0.026073282335115744	0.0
40-41	0.0027757271013574233	1.1416872396328733E-4	0.0	0.036683838118453764	0.0
42-43	0.003039742275522525	1.3557535970640372E-4	0.0	0.04623833320513137	0.0
44-45	0.003382248447412387	1.4271090495410917E-4	0.0	0.0572484795223409	0.0
46-47	0.003838923343265537	1.4271090495410917E-4	0.0	0.0697999036130548	0.0
48-49	0.004010176429210467	1.4271090495410917E-4	0.0	0.08243695424674116	0.0
50-51	0.004010176429210467	1.4271090495410917E-4	0.0	0.09467441434655602	0.0
52-53	0.004017311974458173	1.569819954495201E-4	0.0	0.10567028957327013	0.0
54-55	0.0040672607911921115	1.569819954495201E-4	0.0	0.1173939904152502	0.0
56-57	0.004238513877137042	1.8552417644034192E-4	0.0	0.1294102486123862	0.0
58-59	0.004238513877137042	1.9265972168804738E-4	0.0	0.14129806699506348	0.0
60-61	0.004252784967632453	1.9979526693575283E-4	0.0	0.15237243321950236	0.0
62-63	0.0042884626938709805	1.9979526693575283E-4	0.0	0.16286168473362939	0.0
64-65	0.0043669536915957406	2.1406635743116375E-4	0.0	0.1737219846006371	0.0
66-67	0.004695188772990191	2.1406635743116375E-4	0.0	0.1847107242821035	0.0
68-69	0.004766544225467247	2.1406635743116375E-4	0.0	0.19588498814001026	0.0
70-71	0.0049092551304213555	2.2833744792657466E-4	0.0	0.20662398373780697	0.0
72-73	0.00504483049012776	2.568796289173965E-4	0.0	0.2150439271300994	0.0
74-75	0.005109050397357108	2.8542180990821835E-4	0.0	0.22332829516268543	0.0
76-77	0.005123321487852519	3.139639908990402E-4	0.0	0.23065650013207895	0.0
78-79	0.005151863668843341	3.42506171889862E-4	0.0	0.23764933447483028	0.0
80-81	0.005601403019448785	3.42506171889862E-4	0.0	0.24135981800363715	0.0
82-83	0.005658487381430428	3.42506171889862E-4	0.0	0.24437101809816883	0.0
84-85	0.005772656105393716	3.42506171889862E-4	0.0	0.24731799828547119	0.0
86-87	0.0058939603746047085	3.42506171889862E-4	0.0	0.24761055564062712	0.0
88-89	0.005929638100843236	3.42506171889862E-4	0.0	0.24768904663835187	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	8915	0.0	36.17009	1
GGTATCA	11960	0.0	35.309467	1
TATACAC	11190	0.0	31.385427	3
GTATCAA	19190	0.0	28.788391	1
TTATACA	12015	0.0	28.034197	2
TACACAT	14940	0.0	23.585571	5
ACACATC	14275	0.0	22.942522	6
ATCAACG	24520	0.0	22.102604	3
ATACACA	17010	0.0	22.085733	4
ACATCTC	14645	0.0	22.071085	8
AACGCAG	24635	0.0	22.015196	6
CAACGCA	24675	0.0	21.932274	5
TATCAAC	25140	0.0	21.681293	2
TCAACGC	25050	0.0	21.619455	4
CACATCT	15630	0.0	20.655312	7
TACATGG	25345	0.0	20.570887	2
CATCTCC	16115	0.0	20.130098	9
GTACATG	26475	0.0	20.103765	1
ACATGGG	25735	0.0	19.655157	3
ACGCAGA	27880	0.0	19.369202	7
>>END_MODULE