FastQCFastQC Report
Fri 27 May 2016
SRR2139871_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139871_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3497196
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC44800.042.3586961
TATACAC57850.035.7273863
TTATACA60200.032.6605152
GGTATCA49450.031.8573841
GTATCAA74550.026.8061051
TACACAT78200.026.2754825
ACACATC75300.025.3612486
ACATCTC76900.024.9911068
AACGCAG84250.023.6713266
TACATGG98400.023.5435752
GTACATG100900.023.4394051
ATACACA94650.023.2838064
TCAACGC85100.023.2455164
CAACGCA85200.023.2182315
ATCAACG85150.023.089923
CACATCT82750.022.786177
ACATGGG98850.022.620573
TATCAAC90700.021.7664932
CATCTCC93250.020.5228779
ACGCAGA96200.020.5217537
Produced by FastQC (version 0.11.5)