##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139871_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3497196
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77362950203535	34.0	31.0	34.0	31.0	34.0
2	32.98635135119679	34.0	33.0	34.0	31.0	34.0
3	33.096168759200225	34.0	33.0	34.0	31.0	34.0
4	36.427187666919444	37.0	37.0	37.0	35.0	37.0
5	36.28324777907787	37.0	37.0	37.0	35.0	37.0
6	36.35386549681516	37.0	37.0	37.0	35.0	37.0
7	36.364098266153796	37.0	37.0	37.0	35.0	37.0
8	36.375815081568206	37.0	37.0	37.0	35.0	37.0
9	38.2783018166554	39.0	39.0	39.0	37.0	39.0
10-11	38.18330113611019	39.0	38.5	39.0	37.0	39.0
12-13	38.16912649448301	39.0	39.0	39.0	37.0	39.0
14-15	39.662770974231925	41.0	40.0	41.0	37.5	41.0
16-17	39.63708625424483	41.0	39.0	41.0	37.0	41.0
18-19	39.68688686593488	41.0	40.0	41.0	38.0	41.0
20-21	39.70392509368634	41.0	40.0	41.0	38.0	41.0
22-23	39.65384467412548	41.0	40.0	41.0	37.0	41.0
24-25	39.63760244792657	41.0	40.0	41.0	37.0	41.0
26-27	39.527278192367326	41.0	39.0	41.0	37.0	41.0
28-29	39.46021431457684	41.0	39.0	41.0	37.0	41.0
30-31	39.4012304520268	40.0	39.0	41.0	37.0	41.0
32-33	39.32568721445778	40.0	39.0	41.0	36.0	41.0
34-35	39.301518913841804	40.0	39.0	41.0	36.0	41.0
36-37	39.26146332148906	40.0	39.0	41.0	36.0	41.0
38-39	39.19432312070671	40.0	39.0	41.0	36.0	41.0
40-41	39.155534942357406	40.0	39.0	41.0	35.0	41.0
42-43	39.160363736749	40.0	39.0	41.0	35.0	41.0
44-45	39.11331480054011	40.0	39.0	41.0	35.0	41.0
46-47	39.02399839413066	40.0	39.0	41.0	35.0	41.0
48-49	39.034238143772185	40.0	39.0	41.0	35.0	41.0
50-51	38.9181614213731	40.0	38.5	41.0	35.0	41.0
52-53	38.78313083215518	40.0	38.0	41.0	35.0	41.0
54-55	38.60210299599339	40.0	38.0	41.0	35.0	41.0
56-57	38.4704946804798	40.0	38.0	41.0	35.0	41.0
58-59	38.345672193760734	40.0	37.0	41.0	34.0	41.0
60-61	38.13322601874421	40.0	37.0	41.0	34.0	41.0
62-63	38.00568099008139	39.0	36.5	41.0	34.0	41.0
64-65	37.777390958110914	39.0	36.0	41.0	34.0	41.0
66-67	37.564492712313736	39.0	35.5	40.5	34.0	41.0
68-69	37.32560079467823	38.5	35.0	40.0	34.0	41.0
70-71	36.88937035501586	37.5	35.0	40.0	33.0	41.0
72-73	36.62098134769982	37.0	35.0	39.0	33.0	41.0
74-75	36.251957674775326	36.5	35.0	39.0	33.0	41.0
76-77	34.4504152126716	34.5	33.0	37.5	30.0	39.0
78-79	35.406809591770994	35.0	34.5	37.5	32.0	39.0
80-81	35.307597273730636	35.0	35.0	37.0	32.0	39.0
82-83	35.0236405588701	35.0	35.0	37.0	32.0	39.0
84-85	34.767155475213485	35.0	34.0	36.0	32.0	37.5
86-87	34.561894868488835	35.0	34.0	36.0	32.0	37.0
88-89	34.382679295315214	35.0	34.0	36.0	32.0	37.0
90-91	34.302247611194716	35.0	34.0	35.0	32.0	36.5
92-93	34.176254295548745	35.0	34.0	35.0	32.0	36.0
94-95	34.025447885271355	35.0	34.0	35.0	32.0	36.0
96-97	34.043196986747844	35.0	34.0	35.0	32.0	36.0
98-99	34.0557904407579	35.0	34.0	35.0	32.0	36.0
100-101	32.837937262158206	34.5	32.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	3.0
19	12.0
20	20.0
21	72.0
22	195.0
23	524.0
24	1284.0
25	2635.0
26	4855.0
27	8076.0
28	12133.0
29	17238.0
30	23589.0
31	31868.0
32	43152.0
33	61622.0
34	97113.0
35	163831.0
36	339494.0
37	817174.0
38	1198514.0
39	656330.0
40	17460.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.535046934744294	20.36368564987493	13.02835185674466	25.072915558636115
2	14.570282162640702	21.322272078949265	41.179543694661795	22.92790206374824
3	17.629957640131558	26.730323706714387	31.109826265634748	24.529892387519308
4	11.911514253133081	18.389875774763553	38.97185059115932	30.726759380944046
5	12.579878279627449	38.1405274396974	36.39072559845087	12.888868682224272
6	29.19469046033415	38.55152312851194	19.054654654311175	13.199131756842734
7	25.565853329353	32.8180633856381	23.806014875917736	17.81006840909117
8	24.285313148019156	36.45574912015226	21.98529907960549	17.273638652223095
9	25.447258573587856	16.022931527390245	21.188730164812135	37.34107973420977
10-11	23.722793459764617	26.651922418700995	29.648991774236578	19.97629234729781
12-13	24.691504880291355	24.19917942421298	29.279489165337143	21.829826530158527
14-15	21.858955002807964	25.64578879765389	26.519874779680634	25.975381419857506
16-17	21.76395031905561	28.81821607939618	27.349453676602625	22.068379924945585
18-19	21.78296555297444	27.955439157542212	28.712474222205447	21.5491210672779
20-21	22.38009908024287	27.419354146885194	28.347089394547915	21.85345737832402
22-23	21.954969423037955	27.650090364585605	28.313536155915266	22.08140405646117
24-25	21.937965326910366	27.635954441029902	28.473741320965278	21.95233891109445
26-27	22.00447378002937	27.832448568340318	28.413181555779538	21.749896095850772
28-29	21.73094542675781	27.743814303757215	28.42378378316462	22.101456486320355
30-31	21.95222867934847	27.761002512248844	28.47338739261682	21.813381415785862
32-33	21.41790311882872	27.645264589098716	28.882350349184517	22.05448194288805
34-35	21.521603809480254	27.813246662312274	28.566615321892368	22.098534206315104
36-37	21.708202327987287	27.860760990127098	28.606859182147637	21.824177499737978
38-39	21.662084363283714	27.733260747055382	28.594029719720933	22.01062516993997
40-41	21.7873488266479	27.591299995843006	28.676945513450402	21.94440566405869
42-43	21.96932498139442	27.631148620685188	28.56263336260189	21.836893035318504
44-45	21.69650923221922	28.04641244799642	28.26607812248992	21.99100019729444
46-47	22.206262528148855	27.569685999305843	27.72122903010135	22.502822442443954
48-49	22.00149819519851	27.637832464156787	28.007600124372534	22.353069216272168
50-51	21.79012359460612	27.95006332664717	27.96876948558222	22.291043593164485
52-53	21.86560419475026	28.09426234473423	28.136309999281167	21.90382346123434
54-55	21.88968626147175	28.05576140569371	27.710129611104485	22.344422721730066
56-57	22.305684791764435	28.24505346297904	27.312441536165665	22.13682020909086
58-59	21.7562722622095	28.47944897553515	27.964586166731042	21.799692595524313
60-61	22.289910698240586	28.801529504388878	27.145493263963942	21.76306653340659
62-63	21.841550120421964	28.964712902911348	27.219863075928007	21.97387390073868
64-65	21.89383863303462	28.651075211919803	27.367688281520476	22.087397873525106
66-67	22.028111544240865	28.50933413665775	27.251530134586904	22.21102418451448
68-69	22.093134691815326	28.17830590225862	27.475865011500193	22.252694394425856
70-71	22.191108693657092	27.834688674451762	27.51900200932212	22.45520062256902
72-73	22.20156450941844	27.994765787344683	27.551981531474745	22.25168817176213
74-75	22.185135117238012	27.949923579023782	27.50024616326008	22.364695140478126
76-77	22.063476365539266	27.97475865602731	27.4934529339344	22.468312044499022
78-79	22.181980159686475	27.965103646082305	27.457125248398988	22.395790945832232
80-81	22.12133446207824	27.95755534069023	27.52436839522277	22.39674180200876
82-83	22.223708971821004	27.886819659310312	27.62040635106842	22.269065017800262
84-85	22.467998758268738	27.809303311875855	27.416260644518047	22.30643728533736
86-87	22.106266515666288	27.903716287068498	27.546495532905503	22.443521664359718
88-89	22.272424878446017	27.865782085310965	27.476034188441893	22.385758847801124
90-91	22.360346167080365	27.878980023328836	27.502645594246992	22.2580282153438
92-93	22.36516658711049	27.843407037783575	27.486163497513953	22.305262877591986
94-95	22.20380633610437	27.896120674315387	27.529852201136094	22.370220788444144
96-97	22.181037668280087	27.867068425158415	27.636528697143742	22.315365209417752
98-99	22.455429430277263	28.43467708356811	27.716636871892753	21.393256614261873
100-101	26.579292016727667	35.537964512620704	28.241910838005786	9.640832632645848
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	550.0
1	559.0
2	978.0
3	1447.0
4	2192.5
5	3991.0
6	5336.0
7	5852.0
8	5744.5
9	5042.0
10	4182.0
11	3465.5
12	3844.5
13	5540.5
14	8994.5
15	13315.5
16	16672.5
17	18539.5
18	18820.5
19	17905.5
20	16668.5
21	15870.5
22	16158.0
23	18220.5
24	22140.0
25	27808.0
26	35028.0
27	42698.0
28	50189.0
29	58573.5
30	66871.5
31	74086.0
32	81958.5
33	89928.5
34	97827.0
35	106473.0
36	115947.0
37	124450.5
38	130186.0
39	134798.5
40	139922.0
41	142881.0
42	144137.5
43	146967.0
44	149816.0
45	152794.0
46	156954.0
47	160883.5
48	168335.5
49	168849.5
50	160495.0
51	154616.5
52	147154.5
53	137146.0
54	125029.0
55	112505.5
56	102731.5
57	93238.5
58	81798.0
59	70317.5
60	59434.0
61	48315.0
62	37770.0
63	29454.5
64	22611.5
65	16794.0
66	12405.5
67	9421.0
68	7135.0
69	5235.5
70	3849.0
71	2792.0
72	2009.5
73	1432.0
74	1043.0
75	752.5
76	513.5
77	318.0
78	198.5
79	137.5
80	99.0
81	63.0
82	43.0
83	32.5
84	25.0
85	20.5
86	17.5
87	15.5
88	10.0
89	5.0
90	2.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001830037550082981
3	5.146980609608383E-4
4	0.0
5	0.0
6	5.43292397680885E-4
7	0.0
8	0.0
9	5.7188673440093155E-5
10-11	3.717263773606055E-4
12-13	5.00400892600815E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.722772586030798E-5
28-29	0.0
30-31	7.173339737691051E-5
32-33	2.015989678132848E-4
34-35	4.341846923265961E-5
36-37	8.454629324998429E-4
38-39	1.7675198779704276E-4
40-41	1.0429719336252661E-4
42-43	0.0
44-45	0.0
46-47	1.5501535117022636E-5
48-49	3.145485465576672E-5
50-51	1.4352531013028748E-4
52-53	0.0
54-55	7.53004775523547E-4
56-57	6.649516455475253E-5
58-59	0.0
60-61	0.0010469834689892077
62-63	0.0
64-65	0.0
66-67	3.6077430825134133E-5
68-69	0.0
70-71	0.0
72-73	5.678659427511628E-5
74-75	0.0
76-77	3.8935852015728524E-5
78-79	3.950678153658466E-5
80-81	0.0
82-83	0.0
84-85	2.0667959516427616E-5
86-87	5.872202419850729E-4
88-89	6.388607494390269E-5
90-91	0.0
92-93	2.2007235979189956E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	77.0
20-21	312.0
22-23	635.0
24-25	1002.0
26-27	2100.0
28-29	5041.0
30-31	12118.0
32-33	16189.0
34-35	22014.0
36-37	33228.0
38-39	39377.0
40-41	37750.0
42-43	41451.0
44-45	48155.0
46-47	47368.0
48-49	44566.0
50-51	40748.0
52-53	39476.0
54-55	45970.0
56-57	47082.0
58-59	47450.0
60-61	46916.0
62-63	46086.0
64-65	46954.0
66-67	50557.0
68-69	43769.0
70-71	39997.0
72-73	36746.0
74-75	36681.0
76-77	36677.0
78-79	37975.0
80-81	38594.0
82-83	36136.0
84-85	34974.0
86-87	35918.0
88-89	37132.0
90-91	38695.0
92-93	37674.0
94-95	39850.0
96-97	59293.0
98-99	259049.0
100-101	1885414.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.10023080542794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.5951184456604	78.90855726462102
2	6.75672298216476	11.770242624697799
3	1.5895676247529869	4.153551209904631
4	0.5557438634439062	1.9362167509865784
5	0.22328974962116294	0.9724294364244754
6	0.11169985732647246	0.5837450012441473
7	0.05497969851504482	0.33521211011912727
8	0.03287942206256781	0.22910442003189888
9	0.019872656619878252	0.15578216804775702
>10	0.05868638041416076	0.7287881724693963
>50	6.915081763889798E-4	0.043294568708352035
>100	7.011446567702263E-4	0.16145222636436246
>500	4.66665855495121E-5	0.021624046380443157
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.8594336720046574E-5	0.0	2.8594336720046574E-5	0.0
3	0.0	2.8594336720046574E-5	0.0	2.8594336720046574E-5	0.0
4	0.0	2.8594336720046574E-5	0.0	2.8594336720046574E-5	0.0
5	0.0	5.718867344009315E-5	0.0	5.718867344009315E-5	0.0
6	0.0	5.718867344009315E-5	0.0	5.718867344009315E-5	0.0
7	0.0	5.718867344009315E-5	0.0	5.718867344009315E-5	0.0
8	0.0	5.718867344009315E-5	0.0	8.578301016013973E-5	0.0
9	0.0	1.7156602032027945E-4	0.0	8.578301016013973E-5	0.0
10-11	0.0	1.7156602032027945E-4	0.0	1.7156602032027945E-4	0.0
12-13	0.0	1.7156602032027945E-4	0.0	2.0016035704032602E-4	0.0
14-15	0.0	2.430518621203959E-4	0.0	2.287546937603726E-4	0.0
16-17	0.0	2.5734903048041917E-4	0.0	2.8594336720046576E-4	0.0
18-19	0.0	2.8594336720046576E-4	0.0	3.431320406405589E-4	0.0
20-21	0.0	3.145377039205123E-4	0.0	3.431320406405589E-4	0.0
22-23	0.0	3.145377039205123E-4	0.0	4.432122191607219E-4	0.0
24-25	0.0	3.145377039205123E-4	0.0	6.862640812811178E-4	2.8594336720046574E-5
26-27	0.0	3.145377039205123E-4	0.0	7.148584180011644E-4	2.8594336720046574E-5
28-29	0.0	3.145377039205123E-4	0.0	0.0012009621422419561	2.8594336720046574E-5
30-31	0.0	3.431320406405589E-4	0.0	0.0029595138505248203	2.8594336720046574E-5
32-33	2.8594336720046574E-5	3.431320406405589E-4	0.0	0.006762560634291015	2.8594336720046574E-5
34-35	2.8594336720046574E-5	3.860235457206287E-4	0.0	0.012023918590779585	2.8594336720046574E-5
36-37	2.8594336720046574E-5	4.289150508006986E-4	0.0	0.018500535857870135	2.8594336720046574E-5
38-39	2.8594336720046574E-5	4.289150508006986E-4	0.0	0.027479157587964757	2.8594336720046574E-5
40-41	2.8594336720046574E-5	4.861037242407918E-4	0.0	0.03785890181734167	2.8594336720046574E-5
42-43	2.8594336720046574E-5	5.004008926008151E-4	0.0	0.04683752354743629	2.8594336720046574E-5
44-45	2.8594336720046574E-5	5.575895660409082E-4	0.0	0.05763188565925387	2.8594336720046574E-5
46-47	2.8594336720046574E-5	6.290754078410246E-4	0.0	0.07002753062739406	2.8594336720046574E-5
48-49	2.8594336720046574E-5	6.290754078410246E-4	0.0	0.08139377947361258	2.8594336720046574E-5
50-51	2.8594336720046574E-5	7.00561249641141E-4	0.0	0.09280291982491115	2.8594336720046574E-5
52-53	2.8594336720046574E-5	7.148584180011644E-4	0.0	0.10268226316168724	2.8594336720046574E-5
54-55	2.8594336720046574E-5	7.43452754721211E-4	0.0	0.11260449800354341	2.8594336720046574E-5
56-57	2.8594336720046574E-5	7.720470914412576E-4	0.0	0.12226938381491916	2.8594336720046574E-5
58-59	2.8594336720046574E-5	7.720470914412576E-4	0.0	0.13143386873369406	2.8594336720046574E-5
60-61	2.8594336720046574E-5	7.720470914412576E-4	0.0	0.14036959895870865	2.8594336720046574E-5
62-63	2.8594336720046574E-5	8.006414281613041E-4	0.0	0.14884781979620243	2.8594336720046574E-5
64-65	2.8594336720046574E-5	9.007216066814672E-4	0.0	0.15683993690945547	2.8594336720046574E-5
66-67	2.8594336720046574E-5	9.150187750414904E-4	0.0	0.16550402093562958	2.8594336720046574E-5
68-69	2.8594336720046574E-5	9.436131117615369E-4	0.0	0.1730100343246418	2.8594336720046574E-5
70-71	2.8594336720046574E-5	9.722074484815836E-4	0.0	0.179901269474173	2.8594336720046574E-5
72-73	2.8594336720046574E-5	9.722074484815836E-4	0.0	0.18587748584866276	2.8594336720046574E-5
74-75	2.8594336720046574E-5	0.00100080178520163	0.0	0.19065274008091054	2.8594336720046574E-5
76-77	2.8594336720046574E-5	0.001057990458641723	0.0	0.1948704047471174	2.8594336720046574E-5
78-79	2.8594336720046574E-5	0.0010865847953617697	0.0	0.19844469683712324	2.8594336720046574E-5
80-81	2.8594336720046574E-5	0.0010865847953617697	0.0	0.20017465420868602	2.8594336720046574E-5
82-83	2.8594336720046574E-5	0.0010865847953617697	0.0	0.20097529563684735	2.8594336720046574E-5
84-85	2.8594336720046574E-5	0.0010865847953617697	0.0	0.20160437104468837	2.8594336720046574E-5
86-87	2.8594336720046574E-5	0.0010865847953617697	0.0	0.20180453140172872	2.8594336720046574E-5
88-89	2.8594336720046574E-5	0.0011866649738819328	0.0	0.20190461158024886	2.8594336720046574E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	4480	0.0	42.358696	1
TATACAC	5785	0.0	35.727386	3
TTATACA	6020	0.0	32.660515	2
GGTATCA	4945	0.0	31.857384	1
GTATCAA	7455	0.0	26.806105	1
TACACAT	7820	0.0	26.275482	5
ACACATC	7530	0.0	25.361248	6
ACATCTC	7690	0.0	24.991106	8
AACGCAG	8425	0.0	23.671326	6
TACATGG	9840	0.0	23.543575	2
GTACATG	10090	0.0	23.439405	1
ATACACA	9465	0.0	23.283806	4
TCAACGC	8510	0.0	23.245516	4
CAACGCA	8520	0.0	23.218231	5
ATCAACG	8515	0.0	23.08992	3
CACATCT	8275	0.0	22.78617	7
ACATGGG	9885	0.0	22.62057	3
TATCAAC	9070	0.0	21.766493	2
CATCTCC	9325	0.0	20.522877	9
ACGCAGA	9620	0.0	20.521753	7
>>END_MODULE