FastQCFastQC Report
Fri 27 May 2016
SRR2139870_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139870_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10769332
Sequences flagged as poor quality0
Sequence length19-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC178350.052.038671
TATACAC208150.045.6935733
TTATACA214050.043.8416672
GGTATCA121050.037.0568471
TACACAT286950.033.339235
ATACACA312850.031.0359484
GTATCAA195500.030.1938881
ACACATC317950.029.3270856
ACATCTC342700.027.1859028
CACATCT347200.026.7878157
CATCTCC391100.023.8215489
ATCAACG247800.023.0838053
TCAACGC248600.022.945654
CAACGCA252100.022.5955945
TATCAAC260250.022.163062
AACGCAG266050.021.4257436
CCGTCTT270550.019.39627846-47
GTACATG333450.019.2977681
ACGCAGA295450.019.2802497
TATGCCG272650.019.21301542-43
Produced by FastQC (version 0.11.5)