##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139870_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10769332
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.718019186333933	31.0	31.0	34.0	30.0	34.0
2	31.910435020482236	31.0	31.0	34.0	30.0	34.0
3	31.93175342723207	31.0	31.0	34.0	30.0	34.0
4	35.665869805109544	37.0	35.0	37.0	33.0	37.0
5	35.45733746531354	37.0	35.0	37.0	33.0	37.0
6	35.45564209553573	37.0	35.0	37.0	33.0	37.0
7	35.33235459729536	37.0	35.0	37.0	32.0	37.0
8	35.30010487187135	37.0	35.0	37.0	32.0	37.0
9	36.8629982806733	39.0	37.0	39.0	33.0	39.0
10-11	36.69069283963016	39.0	35.0	39.0	32.0	39.0
12-13	36.63789369665639	39.0	35.0	39.0	32.0	39.0
14-15	37.74565674082663	40.0	37.0	41.0	32.0	41.0
16-17	37.73409813162042	40.0	37.0	41.0	32.0	41.0
18-19	37.70873560217105	39.5	37.0	41.0	32.0	41.0
20-21	37.68163914521157	39.5	37.0	41.0	32.0	41.0
22-23	37.58565947637618	39.0	36.5	41.0	32.0	41.0
24-25	37.46223811983993	39.0	36.0	41.0	32.0	41.0
26-27	37.283006810309175	39.0	36.0	41.0	31.0	41.0
28-29	37.13943268270377	39.0	36.0	40.0	31.0	41.0
30-31	37.00593189696042	39.0	36.0	40.0	31.0	41.0
32-33	37.16741456094756	39.0	36.0	40.0	31.0	41.0
34-35	37.10915740563193	39.0	36.0	40.0	31.0	41.0
36-37	37.011680336985684	39.0	35.5	40.0	30.5	41.0
38-39	36.791948065130875	39.0	35.0	40.0	30.0	41.0
40-41	36.80139453367485	39.0	35.0	40.0	30.0	41.0
42-43	36.6628473356701	38.5	35.0	40.0	30.0	41.0
44-45	36.72736069092647	38.5	35.0	40.0	30.0	41.0
46-47	36.87927085973517	39.0	35.0	40.0	30.0	41.0
48-49	36.80395131546634	39.0	35.0	40.0	30.0	41.0
50-51	36.643422487582576	39.0	35.0	40.0	30.0	41.0
52-53	36.51126399734118	38.5	35.0	40.0	30.0	41.0
54-55	36.33922890386195	38.0	35.0	40.0	29.5	41.0
56-57	36.12644931443536	38.0	34.0	40.0	29.0	41.0
58-59	35.96772619422603	38.0	34.0	40.0	29.0	41.0
60-61	35.715364122420056	37.5	34.0	40.0	28.5	41.0
62-63	35.44893284898496	37.0	34.0	40.0	28.0	41.0
64-65	35.21174663691154	36.5	33.5	39.0	28.0	41.0
66-67	34.9110954950749	36.0	33.0	39.0	28.0	40.5
68-69	34.57378609156919	35.5	33.0	39.0	28.0	40.0
70-71	34.20875254156091	35.0	32.5	38.0	27.0	40.0
72-73	33.9225900774437	35.0	32.0	37.5	27.0	39.5
74-75	33.5747421925179	35.0	32.0	37.0	26.0	39.0
76-77	32.53252698121341	34.0	30.5	36.0	26.0	38.5
78-79	32.90222580873472	34.0	31.0	36.0	26.0	38.0
80-81	32.798384976802765	34.0	31.0	35.5	26.0	37.0
82-83	32.41217444820202	34.0	31.0	35.0	26.0	37.0
84-85	32.19837750559735	34.0	31.0	35.0	26.0	36.5
86-87	32.12211844709623	34.0	31.0	35.0	26.0	36.0
88-89	31.984414599782795	34.0	31.0	35.0	26.0	36.0
90-91	31.87312946482026	34.0	31.0	35.0	25.0	35.0
92-93	31.79993331035938	34.0	31.0	35.0	25.0	35.0
94-95	31.772823817377507	34.0	31.0	35.0	25.0	35.0
96-97	31.708409404248666	34.0	31.0	35.0	25.0	35.0
98-99	31.944232267349452	34.0	31.0	35.0	26.0	35.0
100-101	31.02157386619284	32.5	29.0	34.5	23.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	22.0
20	141.0
21	845.0
22	3540.0
23	11992.0
24	29958.0
25	60873.0
26	106063.0
27	163531.0
28	226774.0
29	295568.0
30	367195.0
31	444422.0
32	538116.0
33	659986.0
34	837183.0
35	1090652.0
36	1502171.0
37	1958487.0
38	1661155.0
39	788040.0
40	22615.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	37.63301196397325	21.821242023182126	14.464054037892044	26.08169197495258
2	14.501803623069105	21.82908095777599	40.00752333724915	23.66159208190576
3	17.086779384273786	27.24831958008166	31.289461593346736	24.375439442297814
4	12.442556779298247	19.233332064297407	37.078174779675244	31.245936376729098
5	12.836153625870203	37.9787065715868	36.080696555738086	13.104443246804909
6	30.123660409020726	38.020194753026466	18.036160460091676	13.819984377861132
7	26.063891427992004	32.1140716991546	22.96184201582791	18.860194857025487
8	24.817147433099844	36.26653909453251	21.23450182425428	17.681811648113367
9	25.50550953392467	15.863035887462656	20.229722697749498	38.401731880863174
10-11	24.334341257192182	26.344424148127292	28.872584669132685	20.448649925547844
12-13	25.16650986337871	23.447053169128782	28.513235547014432	22.873201420478075
14-15	22.51008233379749	24.76406150353615	25.27922808954167	27.446628073124685
16-17	22.669570513511587	28.156060039206388	26.055474320520545	23.118895126761476
18-19	22.831997841248768	27.052204209620612	27.430733177772254	22.685064771358363
20-21	23.23888291269248	26.366991473767182	27.01499761158504	23.37912800195531
22-23	22.972822984052197	26.89407509045832	26.857568540223753	23.27553338526573
24-25	23.00185568814777	27.073815584324368	27.118289295966374	22.80603943156149
26-27	22.933685621931126	27.018053628735224	27.386501955480576	22.661758793853075
28-29	22.453140410971965	26.58415662415764	27.78919723225049	23.1735057326199
30-31	22.87964898317379	26.587974160774536	26.83558057076781	23.696796285283867
32-33	23.23340396440254	26.63315726962726	27.53129228089811	22.602146485072083
34-35	23.02723919731054	27.349989662610664	27.19794183636724	22.424829303711558
36-37	22.597440572186564	26.98785022944617	27.10682196560227	23.307887232764998
38-39	22.631017574781882	26.63966131667933	27.57871577419701	23.150605334341773
40-41	22.708433877242605	26.512219927225978	27.84539222895016	22.93395396658126
42-43	23.290321216027486	26.4054878150287	27.512376118854608	22.7918148500892
44-45	22.640350711906294	27.14510055546244	27.26284748633658	22.951701246294686
46-47	23.4135023397703	26.340413339970294	26.712650895969652	23.533433424289747
48-49	23.15874626359141	26.259471150709	26.74372409117691	23.83805849452267
50-51	22.50827368403958	26.447043276064942	27.400785483436106	23.643897556459372
52-53	22.711085626334494	26.609722023122405	27.72216605511586	22.957026295427244
54-55	22.883223126395773	26.545309205211748	27.010005522958192	23.56146214543429
56-57	23.35576910323168	26.89333350730976	26.529794667198374	23.221102722260188
58-59	22.63208457622942	27.3420821888822	27.431644712299992	22.59418852258839
60-61	23.24302115906964	27.98961576395697	26.051015199261958	22.716347877711435
62-63	22.734777621370622	28.208933936524417	26.180369201101144	22.87591924100382
64-65	22.751402588341914	27.835563683479187	26.290085748821664	23.122947979357235
66-67	22.973723693364917	27.475187658935717	26.223228151099082	23.327860496600284
68-69	23.108362986122287	27.077795385431514	26.49184210688384	23.321999521562358
70-71	23.11191310906326	26.643257771368344	26.6577281292456	23.587100990322796
72-73	23.118460804221886	26.86560256305276	26.488526155346538	23.52741047737882
74-75	22.863650870475585	27.070690240028327	26.605998022105325	23.45966086739076
76-77	23.103608941306277	27.013971178471767	26.464682831985304	23.41773704823665
78-79	23.124078298289284	27.03526061801685	26.439937573312328	23.40072351038154
80-81	23.06767843062505	26.99672162812564	26.399663998528688	23.535935942720617
82-83	23.183491666053477	26.88702001006656	26.509561692097122	23.419926631782843
84-85	23.21826717746292	26.877261567365235	26.395699447558457	23.50877180761339
86-87	22.991140045282396	27.057465145312708	26.570396855322	23.380997954082897
88-89	23.111140670412215	26.796107330791475	26.593878099138262	23.498873899658047
90-91	23.04489842051501	26.836390587197794	26.625594532213466	23.493116460073725
92-93	23.109380441650426	26.91866684622239	26.618977728225566	23.352974983901614
94-95	22.784726751258344	26.99891007933079	26.699150266723766	23.517212902687103
96-97	22.619528293564088	26.943283679734453	26.901866214483327	23.535321812218136
98-99	22.75296552119711	27.629025817738196	26.942732404868373	22.675276256196323
100-101	26.036087233900673	35.65686348569987	27.74401693659444	10.563032343805014
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2572.0
1	2542.0
2	3626.5
3	4916.5
4	6367.0
5	9641.0
6	12065.5
7	13292.5
8	13515.0
9	12627.0
10	11500.5
11	10536.0
12	11690.5
13	15878.5
14	25173.5
15	37419.0
16	46311.0
17	49554.5
18	49327.0
19	47454.0
20	44581.5
21	42726.5
22	43050.5
23	47342.5
24	56628.5
25	70413.0
26	88847.0
27	108354.0
28	126478.0
29	144867.0
30	161638.5
31	178991.5
32	202150.0
33	226098.0
34	250845.5
35	280828.5
36	316305.5
37	343281.0
38	353127.0
39	359848.5
40	371648.5
41	382409.0
42	390826.5
43	406145.5
44	423220.0
45	439662.5
46	452849.5
47	468732.5
48	529243.0
49	567762.0
50	554543.0
51	550750.5
52	532738.0
53	500046.5
54	470824.5
55	438438.0
56	404386.5
57	377138.0
58	347887.0
59	304777.5
60	253020.0
61	202158.0
62	158045.0
63	121952.5
64	93426.5
65	70734.5
66	52432.5
67	39436.0
68	30049.5
69	22606.5
70	16746.0
71	12258.0
72	8946.0
73	6354.5
74	4531.0
75	3261.5
76	2245.5
77	1505.5
78	1054.5
79	750.0
80	530.0
81	375.5
82	267.0
83	191.5
84	143.0
85	97.5
86	69.0
87	55.0
88	35.5
89	22.0
90	17.0
91	14.0
92	11.5
93	6.0
94	2.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0016249847251435835
3	0.0
4	1.8571254001640957E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0015692709631386606
18-19	0.0013278446611173283
20-21	1.3929323263794593E-5
22-23	0.002298731327858204
24-25	1.4864807364174218E-4
26-27	0.0011525932092368448
28-29	5.210134493109272E-4
30-31	1.631532317438759E-4
32-33	0.001006086024941013
34-35	0.015427261052068653
36-37	0.03323885652648454
38-39	0.008583257066969415
40-41	0.005150164568087547
42-43	0.006726923055456578
44-45	0.004809611289198952
46-47	0.0027815310562972328
48-49	0.0012907531059703197
50-51	1.8101762315171958E-4
52-53	9.431035084393618E-5
54-55	0.0042796979043020255
56-57	0.01122453292572788
58-59	0.009268955150316033
60-61	0.007717598613667058
62-63	0.0018417593349202403
64-65	0.0011878834726875168
66-67	0.001326204431926548
68-69	0.0
70-71	0.0
72-73	7.932339021923861E-4
74-75	8.635910886035567E-4
76-77	9.039649581858094E-5
78-79	9.198863125647533E-4
80-81	0.0022570152319729446
82-83	0.004242613548325213
84-85	0.011402101915149137
86-87	0.00725631331239971
88-89	0.06724819137013505
90-91	0.0
92-93	7.655871632469164E-6
94-95	1.1860701282149714E-4
96-97	0.008284458743601949
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	400.0
20-21	1509.0
22-23	2806.0
24-25	4653.0
26-27	8263.0
28-29	16322.0
30-31	38861.0
32-33	50750.0
34-35	68117.0
36-37	103467.0
38-39	116718.0
40-41	115013.0
42-43	123957.0
44-45	141180.0
46-47	142658.0
48-49	135011.0
50-51	125843.0
52-53	124067.0
54-55	137128.0
56-57	151159.0
58-59	159283.0
60-61	167539.0
62-63	165058.0
64-65	171771.0
66-67	181259.0
68-69	141030.0
70-71	137591.0
72-73	131356.0
74-75	130931.0
76-77	130998.0
78-79	142418.0
80-81	148123.0
82-83	140981.0
84-85	141428.0
86-87	148479.0
88-89	164360.0
90-91	180259.0
92-93	198925.0
94-95	254901.0
96-97	316828.0
98-99	878287.0
100-101	4929645.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.90429149957187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.32529864617004	74.05450597248392
2	6.957392489179598	11.535953899997546
3	1.8912388240187519	4.703754442852743
4	0.7695198887758654	2.551860046951699
5	0.37703666570359046	1.5628978819758548
6	0.21222647348562518	1.0556691253067056
7	0.13517203609361086	0.7844439318026755
8	0.08506548441301401	0.564183497306304
9	0.054954828160374364	0.41003919838049013
>10	0.18772964197482933	2.3203587980948353
>50	0.003124256587789921	0.17264457217812632
>100	0.001117799961987204	0.1649754142300682
>500	4.098874964776303E-5	0.02433068110566749
>1k	8.197672510918608E-5	0.09438253733332538
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.285627000820479E-6	0.0	0.0	0.0
3	0.0	9.285627000820479E-6	0.0	0.0	0.0
4	0.0	3.7142508003281915E-5	0.0	9.285627000820479E-6	9.285627000820479E-6
5	0.0	3.7142508003281915E-5	0.0	9.285627000820479E-6	9.285627000820479E-6
6	0.0	3.7142508003281915E-5	0.0	9.285627000820479E-6	1.8571254001640957E-5
7	0.0	3.7142508003281915E-5	0.0	9.285627000820479E-6	1.8571254001640957E-5
8	0.0	4.642813500410239E-5	0.0	9.285627000820479E-6	2.7856881002461433E-5
9	0.0	9.285627000820478E-5	0.0	3.7142508003281915E-5	3.7142508003281915E-5
10-11	0.0	1.0678471050943549E-4	0.0	3.7142508003281915E-5	3.7142508003281915E-5
12-13	0.0	1.1142752400984573E-4	0.0	3.7142508003281915E-5	3.7142508003281915E-5
14-15	0.0	1.6249847251435839E-4	0.0	3.7142508003281915E-5	4.642813500410239E-5
16-17	0.0	1.7642691301558908E-4	0.0	1.0214189700902526E-4	8.35706430073843E-5
18-19	0.0	1.9499816701723003E-4	0.0	1.5785565901394813E-4	9.285627000820478E-5
20-21	0.0	2.0892660751846073E-4	0.0	1.671412860147686E-4	1.067847105094355E-4
22-23	0.0	2.367834885209222E-4	0.0	1.8106972651599934E-4	1.2999877801148668E-4
24-25	0.0	2.5535474252256314E-4	0.0	1.9964098051764029E-4	1.4857003201312766E-4
26-27	0.0	2.5999755602297336E-4	0.0	2.460691155217427E-4	1.4857003201312766E-4
28-29	0.0	2.6928318302379386E-4	0.0	6.685651440590744E-4	1.4857003201312766E-4
30-31	0.0	2.7392599652420414E-4	0.0	0.0022610501746997864	1.4857003201312766E-4
32-33	0.0	2.971400640262553E-4	0.0	0.005682803724502132	1.671412860147686E-4
34-35	0.0	3.203541315283065E-4	0.0	0.009559552997344681	2.2285504801969146E-4
36-37	0.0	3.4356819903035767E-4	0.0	0.01536771268635789	2.274978615201017E-4
38-39	0.0	3.4821101253076794E-4	0.0	0.023641206344088936	2.4142630202133244E-4
40-41	0.0	3.621394530319986E-4	0.0	0.033340043746445926	2.4142630202133244E-4
42-43	0.0	3.8071070703363957E-4	0.0	0.041399967983158106	2.460691155217427E-4
44-45	0.0	4.27138842037742E-4	0.0	0.051391302636040935	2.507119290221529E-4
46-47	0.0	4.549957230402034E-4	0.0	0.061271209764913924	2.7392599652420414E-4
48-49	0.0	4.5963853654061365E-4	0.0	0.07130897255280086	3.0642569102707577E-4
50-51	0.0	4.828526040426649E-4	0.0	0.08069209863712995	3.0642569102707577E-4
52-53	0.0	4.967810445438957E-4	0.0	0.08935558862889545	3.342825720295372E-4
54-55	0.0	5.014238580443058E-4	0.0	0.0985808590542106	3.621394530319986E-4
56-57	0.0	5.014238580443058E-4	0.0	0.10840969523457908	3.6678226653240884E-4
58-59	0.0	5.014238580443058E-4	0.0	0.11779282131890817	3.8535352053404985E-4
60-61	0.0	5.107094850451263E-4	0.0	0.1266838091721938	4.08567588036101E-4
62-63	0.0	5.107094850451263E-4	0.0	0.13555622577147775	4.549957230402034E-4
64-65	0.0	5.757088740508696E-4	0.0	0.1440200747827256	4.549957230402034E-4
66-67	0.0	5.757088740508696E-4	0.0	0.15270213602849275	4.549957230402034E-4
68-69	0.0	5.849945010516901E-4	0.0	0.16202954835081693	4.5963853654061365E-4
70-71	0.0	5.942801280525106E-4	0.0	0.17118053376012551	4.642813500410239E-4
72-73	0.0	5.942801280525106E-4	0.0	0.17858582129327982	4.828526040426649E-4
74-75	0.0	5.989229415529209E-4	0.0	0.18553611310339396	4.828526040426649E-4
76-77	0.0	6.082085685537413E-4	0.0	0.19210569420647444	4.874954175430751E-4
78-79	0.0	6.128513820541515E-4	0.0	0.19778385511747618	4.921382310434854E-4
80-81	0.0	6.128513820541515E-4	0.0	0.20141453527479697	5.107094850451263E-4
82-83	0.0	6.128513820541515E-4	0.0	0.2039634398865222	5.292807390467673E-4
84-85	0.0	6.128513820541515E-4	0.0	0.20606663440220804	5.292807390467673E-4
86-87	0.0	6.128513820541515E-4	0.0	0.20639627416073716	5.339235525471775E-4
88-89	0.0	6.314226360557925E-4	0.0	0.20649377324424578	5.385663660475877E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	17835	0.0	52.03867	1
TATACAC	20815	0.0	45.693573	3
TTATACA	21405	0.0	43.841667	2
GGTATCA	12105	0.0	37.056847	1
TACACAT	28695	0.0	33.33923	5
ATACACA	31285	0.0	31.035948	4
GTATCAA	19550	0.0	30.193888	1
ACACATC	31795	0.0	29.327085	6
ACATCTC	34270	0.0	27.185902	8
CACATCT	34720	0.0	26.787815	7
CATCTCC	39110	0.0	23.821548	9
ATCAACG	24780	0.0	23.083805	3
TCAACGC	24860	0.0	22.94565	4
CAACGCA	25210	0.0	22.595594	5
TATCAAC	26025	0.0	22.16306	2
AACGCAG	26605	0.0	21.425743	6
CCGTCTT	27055	0.0	19.396278	46-47
GTACATG	33345	0.0	19.297768	1
ACGCAGA	29545	0.0	19.280249	7
TATGCCG	27265	0.0	19.213015	42-43
>>END_MODULE