FastQCFastQC Report
Fri 27 May 2016
SRR2139869_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139869_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10409736
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC123900.043.900191
GGTATCA107200.040.6063081
TATACAC165000.035.79293
TTATACA175800.032.1985442
GTATCAA182000.029.9964261
TACACAT222200.026.6513085
ACACATC222500.025.078486
ACATCTC221400.025.0031998
ATCAACG217400.024.4269453
TCAACGC219750.024.0924934
CAACGCA223950.023.6226965
ATACACA267550.023.3518494
AACGCAG228550.023.041636
TATCAAC237000.022.8654772
CACATCT242900.022.7403857
CATCTCC267050.020.6387219
TACATGG260500.020.2622262
GTACATG267700.020.168111
ACGCAGA266100.019.8355267
ACATGGG260650.019.540283
Produced by FastQC (version 0.11.5)