##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139869_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10409736
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.728308383613186	31.0	31.0	34.0	30.0	34.0
2	31.92435533427553	31.0	31.0	34.0	30.0	34.0
3	31.94964521674709	31.0	31.0	34.0	30.0	34.0
4	35.68060265889548	37.0	35.0	37.0	33.0	37.0
5	35.478017598140816	37.0	35.0	37.0	33.0	37.0
6	35.474518950336495	37.0	35.0	37.0	33.0	37.0
7	35.35574879132381	37.0	35.0	37.0	32.0	37.0
8	35.32586494028283	37.0	35.0	37.0	32.0	37.0
9	36.88994889015437	39.0	37.0	39.0	33.0	39.0
10-11	36.72586922473346	39.0	35.0	39.0	32.0	39.0
12-13	36.67898393388651	39.0	35.0	39.0	32.0	39.0
14-15	37.79639214673648	40.0	37.0	41.0	32.0	41.0
16-17	37.79837058307723	40.0	37.0	41.0	32.0	41.0
18-19	37.777109861383614	40.0	37.0	41.0	32.0	41.0
20-21	37.74928463654956	40.0	37.0	41.0	32.0	41.0
22-23	37.664937645933406	39.0	37.0	41.0	32.0	41.0
24-25	37.554799567394866	39.0	36.0	41.0	32.0	41.0
26-27	37.38089491399208	39.0	36.0	41.0	31.5	41.0
28-29	37.230354522837786	39.0	36.0	40.5	31.0	41.0
30-31	37.0845515539914	39.0	36.0	40.0	31.0	41.0
32-33	37.257076666823735	39.0	36.0	40.0	31.0	41.0
34-35	37.22100721697777	39.0	36.0	41.0	31.0	41.0
36-37	37.10727637701385	39.0	36.0	40.5	31.0	41.0
38-39	36.91145057949854	39.0	35.0	40.0	30.0	41.0
40-41	36.90393700598557	39.0	35.0	40.0	30.0	41.0
42-43	36.78579461868025	39.0	35.0	40.0	30.0	41.0
44-45	36.84881406956431	39.0	35.0	40.0	30.5	41.0
46-47	37.00617302243347	39.0	35.5	40.0	31.0	41.0
48-49	36.93927031408866	39.0	35.0	40.0	30.5	41.0
50-51	36.8009034089893	39.0	35.0	40.0	30.0	41.0
52-53	36.66089026666269	39.0	35.0	40.0	30.0	41.0
54-55	36.523831581104645	38.5	35.0	40.0	30.0	41.0
56-57	36.34394123901812	38.0	35.0	40.0	29.5	41.0
58-59	36.187725239976885	38.0	34.0	40.0	29.0	41.0
60-61	35.9778517067482	38.0	34.0	40.0	29.0	41.0
62-63	35.72500550128977	37.5	34.0	40.0	28.5	41.0
64-65	35.494860341463905	37.0	34.0	40.0	28.0	41.0
66-67	35.207468833835776	36.5	34.0	39.0	28.0	41.0
68-69	34.87632939843581	36.0	33.0	39.0	28.0	40.0
70-71	34.5164942990378	35.5	33.0	39.0	28.0	40.0
72-73	34.22417062151875	35.0	32.5	38.0	28.0	40.0
74-75	33.8683519876034	35.0	32.0	37.0	27.0	39.5
76-77	32.80194894014324	34.0	31.0	36.0	26.0	39.0
78-79	33.14960627333346	34.5	31.5	36.0	26.0	39.0
80-81	33.02541384306819	34.0	32.0	36.0	26.0	38.0
82-83	32.61026192825071	34.0	31.0	35.5	26.0	37.0
84-85	32.37343499395785	34.0	31.0	35.0	26.0	37.0
86-87	32.28063750434451	34.0	31.0	35.0	26.0	36.0
88-89	32.12009588674428	34.0	31.0	35.0	26.0	36.0
90-91	31.993769242058793	34.0	31.0	35.0	26.0	36.0
92-93	31.90901389403186	34.0	31.0	35.0	26.0	35.0
94-95	31.8694680442752	34.0	31.0	35.0	26.0	35.0
96-97	31.79748976376071	34.0	31.0	35.0	25.5	35.0
98-99	32.02480982156892	34.0	31.0	35.0	26.5	35.0
100-101	31.129144090774272	33.0	29.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	4.0
19	26.0
20	136.0
21	709.0
22	3220.0
23	10456.0
24	26746.0
25	54732.0
26	96300.0
27	148753.0
28	209518.0
29	273528.0
30	342169.0
31	417576.0
32	505943.0
33	622221.0
34	789030.0
35	1026646.0
36	1410834.0
37	1897265.0
38	1742930.0
39	807649.0
40	23344.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.755385919489214	21.935513062002727	14.740450670410855	25.568650348097204
2	14.226491446256212	22.12337964381704	40.4541329853979	23.19599592452885
3	16.952046997550564	27.092930520606963	31.548375779823	24.406646702019476
4	12.30254298524823	19.13100757425694	38.08265417733057	30.483795263164264
5	12.640186071961864	38.0752019071377	36.38016372365255	12.904448297247884
6	29.839123681907015	38.68452571707871	18.210836470780816	13.265514130233466
7	25.675607911670383	32.65244190630771	23.513775949745508	18.1581742322764
8	24.245725347885863	37.183959324232625	21.277014133691765	17.29330119418975
9	25.137966995512663	16.209075811336618	20.858905547652697	37.79405164549802
10-11	23.93042436426822	26.72196970220955	29.34763667397521	19.999969259547022
12-13	24.729719370404784	24.037751773916263	29.13749685870996	22.09503199696899
14-15	21.924825951397807	25.44914203395744	26.22845574565964	26.397576268985112
16-17	22.059415107691784	28.64517786312748	26.931157673190153	22.36424935599058
18-19	22.057359662925883	27.85242055875453	28.26469463705548	21.825525141264105
20-21	22.624018271543076	27.147573029683763	28.083587083416273	22.14482161535689
22-23	22.38043693042285	27.36947530789613	27.822026277050764	22.42806148463026
24-25	22.433907268830893	27.579004790196098	27.91865393202231	22.068434008950696
26-27	22.4559154900461	27.72186963802121	27.844761564566817	21.97745330736587
28-29	22.183900701412142	27.763614639813206	27.871413163391072	22.181071495383584
30-31	22.90075018528673	27.430191794358848	27.76433697479498	21.904721045559445
32-33	21.943109825357602	27.95924044848877	27.88889530110185	22.208754425051776
34-35	22.332046070796622	27.84460861959394	27.98250252353427	21.840842786075164
36-37	22.156612065247593	27.822298780911325	27.864501562486772	22.156587591354313
38-39	22.05577716957368	27.374105185471592	28.235861235068228	22.334256409886503
40-41	22.261666661842643	27.292538588286185	28.24507547785039	22.20071927202078
42-43	22.502375152987387	27.29438215234475	28.00213966751842	22.20110302714944
44-45	22.182418397978214	27.69783414470633	27.767584544226025	22.35216291308943
46-47	22.55224285333989	27.24147470939866	27.389937435523805	22.81634500173764
48-49	22.39222428081633	27.248352947060184	27.56768782883	22.791734943293484
50-51	22.096693474818817	27.495513893471124	27.71258527690578	22.69520735480428
52-53	22.296278853973476	27.49265132688809	27.816760294563814	22.394309524574624
54-55	22.30504870133492	27.404608517291095	27.560046008234973	22.730296773139017
56-57	22.552431719516026	27.597402364464017	27.220542007600674	22.62962390841928
58-59	22.193286673991636	27.92693902589849	27.7156814552194	22.164092844890476
60-61	22.58765653837195	28.13331351848158	27.048239871069953	22.230790072076523
62-63	22.27364391566002	28.310168425026138	27.059364539553187	22.356823119760655
64-65	22.252090386372817	28.118466624666645	27.13844710663926	22.49099588232129
66-67	22.41625173901304	27.8969081263	27.06692925155653	22.61991088313043
68-69	22.503809025910133	27.670068459659486	27.248870432043244	22.577252082387133
70-71	22.4525312806685	27.416866870075847	27.29836780302569	22.832234046229967
72-73	22.51373222945694	27.510721457584243	27.263791047092116	22.711755265866703
74-75	22.282336300473577	27.634428085520884	27.300036832628795	22.783198781376743
76-77	22.413504194403437	27.576631849051687	27.211201614146802	22.798662342398075
78-79	22.500332222318555	27.57471637055662	27.16267491818489	22.762276488939932
80-81	22.490243356247813	27.485971590201874	27.191101446244165	22.832683607306144
82-83	22.42005010305905	27.51804047215597	27.22891057774182	22.83299884704316
84-85	22.608229511886766	27.466650115185466	27.19318762258266	22.73193275034511
86-87	22.39515577832204	27.483396935695165	27.301403998405192	22.820043287577604
88-89	22.450737119943682	27.338303656092794	27.355364393774632	22.85559483018889
90-91	22.453235092886086	27.38243850677441	27.3852548282964	22.779071572043105
92-93	22.449425702305422	27.362525922162877	27.40733963287671	22.780708742654987
94-95	22.205671622257704	27.45254022380612	27.516538569870104	22.82524958406607
96-97	21.99976681527383	27.398809537552143	27.732491273713023	22.868932373461007
98-99	22.182794158148962	28.032808183890356	27.781954566449773	22.002443091510905
100-101	25.05568518707058	36.05634974694693	28.83191897560827	10.056046090374217
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1785.0
1	1726.5
2	2593.5
3	3716.5
4	5087.0
5	7890.0
6	10025.0
7	11338.0
8	11932.0
9	11547.0
10	10756.5
11	10273.0
12	11884.5
13	16292.5
14	25557.5
15	37742.0
16	47052.5
17	51137.0
18	51477.0
19	50246.0
20	48413.0
21	47609.5
22	49345.5
23	55575.0
24	66848.5
25	82621.0
26	103070.5
27	125409.5
28	146920.0
29	167614.0
30	185345.0
31	202815.0
32	223665.5
33	246181.0
34	271353.5
35	297069.5
36	324324.5
37	347006.0
38	360715.0
39	373689.5
40	389486.0
41	403403.0
42	413055.0
43	423704.0
44	435781.5
45	449586.0
46	470370.0
47	492055.0
48	494354.0
49	489184.0
50	487661.5
51	476566.0
52	453540.5
53	423284.5
54	393849.5
55	364211.0
56	334440.5
57	304861.0
58	273826.0
59	239286.0
60	200123.5
61	161467.5
62	127065.5
63	97664.0
64	73493.0
65	54764.0
66	40459.5
67	30131.0
68	22455.5
69	16528.5
70	11946.5
71	8598.5
72	6175.0
73	4333.0
74	3016.0
75	2094.5
76	1445.0
77	1013.0
78	737.5
79	522.5
80	359.5
81	246.0
82	172.0
83	119.5
84	87.5
85	67.0
86	48.5
87	35.5
88	25.0
89	14.5
90	9.0
91	8.5
92	8.0
93	5.5
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0013641076008075517
3	1.9212783109965517E-5
4	1.9212783109965517E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0013881235796950086
18-19	0.0014985970825773102
20-21	4.803446568982176E-6
22-23	0.0023684403450409232
24-25	1.4416122530121767E-4
26-27	0.0011778486570241725
28-29	4.619090547262149E-4
30-31	1.4943427557589318E-4
32-33	0.0010351881243992342
34-35	0.015839151593025465
36-37	0.034466937062169224
38-39	0.00856618596399307
40-41	0.005010080541535813
42-43	0.0067471527696078785
44-45	0.004756294754526454
46-47	0.002715802447460474
48-49	0.0012424103536712414
50-51	2.0175359981515123E-4
52-53	6.985681234230161E-5
54-55	0.004543158482125854
56-57	0.01137306124070662
58-59	0.009414851572708669
60-61	0.007959065258702902
62-63	0.0016937309065079037
64-65	0.0011514234648822893
66-67	0.0012675471575374572
68-69	6.083247909324567E-6
70-71	1.8548321778759328E-5
72-73	8.229689503238351E-4
74-75	9.573455946753203E-4
76-77	1.0379687004430764E-4
78-79	9.369439369698019E-4
80-81	0.002486649046853979
82-83	0.004638195726789856
84-85	0.012181727924701983
86-87	0.007710005273131031
88-89	0.06993356420501248
90-91	7.450585481908339E-6
92-93	0.0
94-95	1.2644754781851109E-4
96-97	0.007964586447505874
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	298.0
20-21	1276.0
22-23	2322.0
24-25	4123.0
26-27	7187.0
28-29	14118.0
30-31	33944.0
32-33	44708.0
34-35	61892.0
36-37	92825.0
38-39	102535.0
40-41	102469.0
42-43	112812.0
44-45	130299.0
46-47	129985.0
48-49	122454.0
50-51	113901.0
52-53	112586.0
54-55	124862.0
56-57	134055.0
58-59	136715.0
60-61	141756.0
62-63	137136.0
64-65	141345.0
66-67	151899.0
68-69	132551.0
70-71	129627.0
72-73	124662.0
74-75	126845.0
76-77	127256.0
78-79	136482.0
80-81	142278.0
82-83	136439.0
84-85	137247.0
86-87	145601.0
88-89	159613.0
90-91	177475.0
92-93	193351.0
94-95	249310.0
96-97	309434.0
98-99	870010.0
100-101	4954053.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.64934594958723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.98659691820694	76.17306573355359
2	6.802981588665434	11.517358839752257
3	1.7485903900289141	4.440510985490437
4	0.6793125785023623	2.300134618622104
5	0.310415938176557	1.3138253069486527
6	0.16221634557970527	0.8238904533392559
7	0.09044883138942318	0.5359504093313417
8	0.05513347616239292	0.3733610157658975
9	0.039544014872298014	0.3012637495644699
>10	0.1179388098910212	1.5212103197535278
>50	0.004316876835586747	0.2559254459953486
>100	0.00238179988904584	0.35812378774275894
>500	1.0202709857387941E-4	0.05850340726763981
>1k	2.0404701752309687E-5	0.026875926872713646
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	9.606391554982759E-6	0.0	0.0	0.0
5	0.0	9.606391554982759E-6	0.0	0.0	0.0
6	0.0	9.606391554982759E-6	0.0	0.0	0.0
7	0.0	9.606391554982759E-6	0.0	0.0	1.9212783109965517E-5
8	0.0	9.606391554982759E-6	0.0	0.0	4.803195777491379E-5
9	0.0	2.8819174664948276E-5	0.0	9.606391554982759E-6	4.803195777491379E-5
10-11	0.0	2.8819174664948276E-5	0.0	9.606391554982759E-6	4.803195777491379E-5
12-13	0.0	2.8819174664948276E-5	0.0	1.9212783109965517E-5	4.803195777491379E-5
14-15	0.0	2.8819174664948276E-5	0.0	1.9212783109965517E-5	5.283515355240517E-5
16-17	0.0	2.8819174664948276E-5	0.0	1.9212783109965517E-5	7.685113243986207E-5
18-19	0.0	5.283515355240517E-5	0.0	3.3622370442439654E-5	1.0567030710481034E-4
20-21	0.0	7.685113243986207E-5	0.0	4.803195777491379E-5	1.7771824376718102E-4
22-23	0.0	7.685113243986207E-5	0.0	6.724474088487931E-5	1.921278310996552E-4
24-25	0.0	8.165432821735346E-5	0.0	7.204793666237068E-5	2.1134061420962068E-4
26-27	4.803195777491379E-6	8.645752399484483E-5	0.0	1.2968628599226723E-4	2.1134061420962068E-4
28-29	9.606391554982759E-6	8.645752399484483E-5	0.0	8.453624568384827E-4	2.1134061420962068E-4
30-31	9.606391554982759E-6	1.0567030710481034E-4	0.0	0.0025937257198453446	2.1134061420962068E-4
32-33	9.606391554982759E-6	1.0567030710481034E-4	0.0	0.005951159568311819	2.2094700576460343E-4
34-35	9.606391554982759E-6	1.2007989443728448E-4	0.0	0.010437344424488768	2.2094700576460343E-4
36-37	9.606391554982759E-6	1.3448948176975861E-4	0.0	0.017397175106073776	2.305533973195862E-4
38-39	9.606391554982759E-6	1.440958733247414E-4	0.0	0.027700030048792783	2.4976618042955173E-4
40-41	9.606391554982759E-6	1.440958733247414E-4	0.0	0.03825265117194135	2.4976618042955173E-4
42-43	9.606391554982759E-6	1.440958733247414E-4	0.0	0.04704730264052806	2.545693762070431E-4
44-45	9.606391554982759E-6	1.5370226487972414E-4	0.0	0.058397254262740186	2.5937257198453445E-4
46-47	1.9212783109965517E-5	1.633086564347069E-4	0.0	0.06957909403274012	2.5937257198453445E-4
48-49	1.9212783109965517E-5	1.633086564347069E-4	0.0	0.08103951915783456	2.833885508719914E-4
50-51	1.9212783109965517E-5	1.7291504798968966E-4	0.0	0.09179867769941524	2.929949424269741E-4
52-53	1.9212783109965517E-5	1.7291504798968966E-4	0.0	0.10170286739260245	3.3142050864690516E-4
54-55	1.9212783109965517E-5	1.7291504798968966E-4	0.0	0.11257249943706546	3.650428790893448E-4
56-57	1.9212783109965517E-5	1.7291504798968966E-4	0.0	0.12313953014754649	3.842556621993104E-4
58-59	1.9212783109965517E-5	1.7291504798968966E-4	0.0	0.13219835738389524	3.986652495317845E-4
60-61	1.9212783109965517E-5	1.825214395446724E-4	0.0	0.14086332256648967	4.0346844530927587E-4
62-63	1.9212783109965517E-5	1.825214395446724E-4	0.0	0.14930253754754202	4.2268122841924137E-4
64-65	1.9212783109965517E-5	2.1134061420962068E-4	0.0	0.15778978448636932	4.2268122841924137E-4
66-67	1.9212783109965517E-5	2.1134061420962068E-4	0.0	0.1668630213100505	4.2268122841924137E-4
68-69	1.9212783109965517E-5	2.1614380998711207E-4	0.0	0.17627248183815614	4.2268122841924137E-4
70-71	1.9212783109965517E-5	2.2094700576460343E-4	0.0	0.1856531231915968	4.3228761997422414E-4
72-73	1.9212783109965517E-5	2.2094700576460343E-4	0.0	0.19304043829737855	4.611067946391724E-4
74-75	1.9212783109965517E-5	2.2094700576460343E-4	0.0	0.19979373156053143	4.611067946391724E-4
76-77	1.9212783109965517E-5	2.305533973195862E-4	0.0	0.20622040751081488	4.851227735266293E-4
78-79	1.9212783109965517E-5	2.305533973195862E-4	0.0	0.21208030635935435	4.899259693041207E-4
80-81	1.9212783109965517E-5	2.305533973195862E-4	0.0	0.21571152236713786	5.091387524140862E-4
82-83	1.9212783109965517E-5	2.305533973195862E-4	0.0	0.21827162571654074	5.283515355240517E-4
84-85	1.9212783109965517E-5	2.305533973195862E-4	0.0	0.22066842040950896	5.331547313015431E-4
86-87	1.9212783109965517E-5	2.305533973195862E-4	0.0	0.2210238568970433	5.475643186340172E-4
88-89	1.9212783109965517E-5	2.4015978887456896E-4	0.0	0.22108149524637322	5.667771017439827E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	12390	0.0	43.90019	1
GGTATCA	10720	0.0	40.606308	1
TATACAC	16500	0.0	35.7929	3
TTATACA	17580	0.0	32.198544	2
GTATCAA	18200	0.0	29.996426	1
TACACAT	22220	0.0	26.651308	5
ACACATC	22250	0.0	25.07848	6
ACATCTC	22140	0.0	25.003199	8
ATCAACG	21740	0.0	24.426945	3
TCAACGC	21975	0.0	24.092493	4
CAACGCA	22395	0.0	23.622696	5
ATACACA	26755	0.0	23.351849	4
AACGCAG	22855	0.0	23.04163	6
TATCAAC	23700	0.0	22.865477	2
CACATCT	24290	0.0	22.740385	7
CATCTCC	26705	0.0	20.638721	9
TACATGG	26050	0.0	20.262226	2
GTACATG	26770	0.0	20.16811	1
ACGCAGA	26610	0.0	19.835526	7
ACATGGG	26065	0.0	19.54028	3
>>END_MODULE