FastQCFastQC Report
Fri 27 May 2016
SRR2139868_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139868_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3684110
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC86650.056.9558071
TATACAC100350.050.3061563
TTATACA101200.049.1525082
GGTATCA70150.035.760151
TACACAT143300.035.2555775
ATACACA149800.034.246664
ACACATC154850.032.096856
GTATCAA117500.031.8085331
ACATCTC164850.029.8894988
CACATCT168550.029.4185357
GTGCGCT14800.029.2594059
TTGTGCG16300.026.8061627
CATCTCC186600.026.4265029
AACGCAG142200.025.734016
ATCAACG142950.025.5717053
CAACGCA143750.025.4293925
TCAACGC145750.025.1072124
TATCAAC150200.024.675712
ACGCAGA158800.022.9702327
TGCGCGA4303.6561687E-1022.88969294-95
Produced by FastQC (version 0.11.5)