##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139868_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3684110
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82419580305691	34.0	31.0	34.0	31.0	34.0
2	33.02378132031888	34.0	33.0	34.0	31.0	34.0
3	33.12121272166141	34.0	33.0	34.0	31.0	34.0
4	36.05414740602208	37.0	35.0	37.0	34.0	37.0
5	35.92917013878522	37.0	35.0	37.0	34.0	37.0
6	36.43667018628651	37.0	37.0	37.0	35.0	38.0
7	36.44354918827071	37.0	37.0	37.0	35.0	38.0
8	36.43913591070842	37.0	37.0	37.0	35.0	38.0
9	38.10649926305132	39.0	38.0	39.0	37.0	39.0
10-11	38.01925770403164	39.0	38.0	39.0	37.0	39.0
12-13	37.99330788168649	39.0	38.0	39.0	37.0	39.0
14-15	39.26651145052672	40.0	38.0	41.0	37.0	41.0
16-17	39.21461099152848	40.0	38.0	41.0	37.0	41.0
18-19	39.29080198473987	40.0	38.0	41.0	37.0	41.0
20-21	39.30442663313032	40.0	38.0	41.0	37.0	41.0
22-23	39.246725795714156	40.0	38.0	41.0	37.0	41.0
24-25	39.22526908368882	40.0	38.0	41.0	37.0	41.0
26-27	39.090956916651784	40.0	38.0	41.0	36.5	41.0
28-29	38.998031566982874	40.0	38.0	41.0	36.0	41.0
30-31	38.94983233080606	40.0	38.0	41.0	36.0	41.0
32-33	38.829831683106335	40.0	38.0	41.0	35.0	41.0
34-35	38.79535408726588	40.0	38.0	41.0	35.0	41.0
36-37	38.75171941712601	40.0	38.0	41.0	35.0	41.0
38-39	38.6469217509738	40.0	38.0	41.0	35.0	41.0
40-41	38.65870278699401	40.0	38.0	41.0	35.0	41.0
42-43	38.684049883658275	40.0	38.0	41.0	35.0	41.0
44-45	38.60688230718601	40.0	38.0	41.0	35.0	41.0
46-47	38.51229070848623	40.0	38.0	41.0	35.0	41.0
48-49	38.484384916803194	40.0	38.0	41.0	35.0	41.0
50-51	38.31560817295051	40.0	38.0	41.0	35.0	41.0
52-53	38.17923920661971	39.5	37.5	41.0	34.5	41.0
54-55	37.945342445812116	39.0	37.0	41.0	34.0	41.0
56-57	37.79337397149055	39.0	37.0	41.0	34.0	41.0
58-59	37.66109613562879	39.0	36.5	41.0	34.0	41.0
60-61	37.420128012820605	38.0	36.0	40.5	33.5	41.0
62-63	37.29932476533838	38.0	35.5	40.0	33.0	41.0
64-65	37.09610673700606	38.0	35.0	40.0	33.0	41.0
66-67	36.89178113614956	38.0	35.0	40.0	33.0	41.0
68-69	36.66094554217811	37.5	35.0	39.5	33.0	41.0
70-71	36.273967139584556	37.0	35.0	39.0	33.0	41.0
72-73	36.02356210935877	37.0	35.0	39.0	33.0	40.5
74-75	35.7302095977022	36.0	35.0	38.0	32.0	40.0
76-77	34.234804288753494	34.5	33.0	37.5	30.0	39.0
78-79	35.07272440109482	35.0	34.5	38.0	31.5	39.0
80-81	35.00878231100897	35.0	35.0	37.0	32.0	39.0
82-83	34.79297279491381	35.0	34.5	37.0	32.0	38.0
84-85	34.59945805456532	35.0	34.0	36.5	32.0	38.0
86-87	34.463009627052166	35.0	34.0	36.0	31.5	38.0
88-89	34.33651405857982	35.0	34.0	36.0	31.0	38.0
90-91	34.279007366627674	35.0	34.0	36.0	31.0	38.0
92-93	34.194058507568954	35.0	34.0	35.5	31.0	38.0
94-95	34.063775132278764	35.0	34.0	35.0	31.0	38.0
96-97	34.08850036413487	35.0	34.0	35.0	31.0	38.0
98-99	34.08405395099147	35.0	34.0	35.0	31.0	37.5
100-101	32.76029672423894	34.0	32.0	35.0	28.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	3.0
16	9.0
17	10.0
18	28.0
19	52.0
20	98.0
21	324.0
22	770.0
23	1731.0
24	3214.0
25	5460.0
26	8632.0
27	12036.0
28	16866.0
29	24434.0
30	33230.0
31	40957.0
32	53888.0
33	76577.0
34	121511.0
35	203226.0
36	423892.0
37	1111747.0
38	1005144.0
39	527329.0
40	12942.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.17814071783958	20.14280246789591	13.20408456859323	25.474972245671275
2	14.693947792938284	20.54330805054531	41.588833626804764	23.17391052971164
3	17.339691120550498	27.015293596167954	31.42582525784515	24.219190025436397
4	11.736755960055481	18.93689384953218	37.127365903841095	32.198984286571246
5	12.64872655811037	38.190743490286664	35.65143277480857	13.509097176794397
6	29.02092074740764	37.56815108409286	18.909886664129637	14.501041504369866
7	25.623230576720026	32.860962349115525	22.416920233109217	19.098886841055236
8	25.106959347033612	35.29294727899004	21.728992891091742	17.871100482884607
9	25.77236449844453	15.31515489905429	20.033229201416137	38.87925140108504
10-11	24.18519754561394	25.966121698429074	28.681752130134413	21.166928625822575
12-13	25.413764780415722	23.62538944403759	28.032004034637513	22.928841740909174
14-15	22.768076957528415	24.87933313608986	25.32005016136869	27.03253974501304
16-17	22.352929201353923	28.497547575941002	26.026503008867813	23.123020213837265
18-19	22.368088900711435	26.765813181473952	27.61289700904696	23.253200908767653
20-21	23.108701146021282	25.93026857218707	27.679308939856345	23.281721341935302
22-23	22.40903609453416	27.150091733555154	27.382791990430828	23.058080181479866
24-25	22.696736946421176	27.41822814858745	27.759885267075425	22.125149637915957
26-27	22.876755160046255	27.3288833556946	27.80589951638276	21.988461967876386
28-29	21.411061378987785	26.646779860121605	28.210744857891818	23.731413902998792
30-31	22.757502666669573	26.261214736157857	28.724421239995902	22.256861357176668
32-33	21.683589135223063	27.70771028046986	28.1709882885128	22.437712295794274
34-35	21.71144672025605	27.678021461118473	29.178570975599133	21.43196084302635
36-37	22.94253192628268	27.024640260430736	27.66912512521645	22.363702688070134
38-39	22.211132064576734	26.232911149950915	28.467225039751337	23.08873174572101
40-41	22.088558758992747	26.086904966506275	29.157050140513785	22.667486133987193
42-43	22.853417739005124	25.990553449580823	28.751683496686148	22.404345314727905
44-45	21.914209358155546	27.047306093372136	28.404627487383298	22.63385706108902
46-47	23.133342941564443	26.0903583795374	27.060565230385773	23.715733448512378
48-49	22.71506777873798	26.245748558852217	27.430192489454264	23.608991172955545
50-51	22.150139849362525	26.695414339712332	27.857318370679106	23.29712744024604
52-53	22.105898661024334	26.994116322234934	28.573829303297295	22.326155713443438
54-55	22.136506855935554	27.242833431534276	27.27794013453795	23.34271957799223
56-57	23.149260104183185	27.686214230235894	26.091764160495785	23.072761505085133
58-59	21.911545735311382	28.418227802867506	27.773193784359542	21.89703267746157
60-61	23.033002198744523	29.31663888555335	25.755670026858468	21.89468888884366
62-63	22.13355518103251	29.65414711081972	25.933402004880975	22.278895703266798
64-65	22.26806098996481	29.26102580160432	26.088227702018834	22.382685506412038
66-67	22.48272608838918	28.51968451243525	26.221830558184294	22.77575884099127
68-69	22.681457806082346	27.909541137585002	26.398188633384056	23.010812422948597
70-71	22.942970461205135	27.251485287519223	26.633806785034338	23.17173746624131
72-73	22.67232680920393	27.41654041144289	26.74548489720099	23.16564788215219
74-75	22.750681012400896	27.372184220097097	26.840848640648524	23.03628612685348
76-77	22.77480079922957	27.362096899624134	26.53187988007579	23.33122242107051
78-79	22.55460445808833	27.527073605243242	26.50108072112747	23.41724121554096
80-81	22.752735537441527	27.380680243692456	26.651045987946944	23.215538230919076
82-83	23.03922696422409	27.185279692124283	26.728527655480377	23.046965688171255
84-85	22.941872342144226	27.405698931702737	26.502232572278267	23.15019615387477
86-87	22.825697212876513	27.307360328462043	26.719336716796526	23.14760574186491
88-89	23.033267298118798	27.148532676450948	26.51929855537262	23.298901470057633
90-91	22.8659780219289	27.071404703392147	26.732893682505942	23.329723592173007
92-93	23.231796702146063	27.01035245700134	26.629214506186887	23.128636334665707
94-95	22.721933898791868	27.213798912893562	26.799730460282877	23.26453672803169
96-97	22.973478171368537	27.208854963999496	26.670515553683693	23.14715131094827
98-99	23.253245179019657	27.93159282106859	26.81462557800452	22.00053642190723
100-101	27.935571566599204	35.04135509122591	27.47550408769164	9.547569254483241
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	850.0
1	871.5
2	1450.5
3	2109.5
4	3080.5
5	5374.5
6	7020.0
7	7581.5
8	7368.0
9	6463.5
10	5452.0
11	4642.5
12	5210.0
13	7514.0
14	12471.5
15	18626.5
16	23056.5
17	25171.5
18	25085.5
19	23184.0
20	20490.0
21	18034.0
22	16784.5
23	17563.0
24	20315.5
25	24830.0
26	30795.0
27	37167.0
28	43885.5
29	51575.0
30	58402.0
31	64165.0
32	71219.0
33	78398.5
34	85890.5
35	94606.5
36	102849.0
37	111080.5
38	117423.5
39	121342.0
40	125772.5
41	129868.0
42	134558.5
43	143319.5
44	153880.0
45	168498.5
46	190763.5
47	211373.0
48	212103.0
49	204319.5
50	200723.5
51	192995.5
52	182234.5
53	169999.5
54	158297.0
55	147435.5
56	135996.0
57	120977.0
58	105614.0
59	89363.5
60	72166.5
61	58002.0
62	46242.5
63	36403.0
64	27639.5
65	20723.0
66	15495.5
67	11942.0
68	9228.0
69	6791.0
70	4872.0
71	3353.0
72	2306.0
73	1457.0
74	917.5
75	622.5
76	433.0
77	292.5
78	203.0
79	149.5
80	106.5
81	72.5
82	50.0
83	33.0
84	22.5
85	12.5
86	8.5
87	8.5
88	6.5
89	4.0
90	3.5
91	3.0
92	2.5
93	1.0
94	0.0
95	0.0
96	0.0
97	1.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0013300362909902258
3	5.157283577309038E-4
4	0.0
5	0.0
6	4.3429756440497164E-4
7	0.0
8	0.0
9	2.7143597775310728E-5
10-11	3.1215137441607333E-4
12-13	3.257231733037287E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	2.716492204482321E-5
28-29	0.0
30-31	1.3633278177634253E-5
32-33	2.466060499862243E-4
34-35	0.0
36-37	5.840966340597039E-4
38-39	1.549208277109983E-4
40-41	4.2859151114509365E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	1.5473461620708938E-4
52-53	0.0
54-55	5.901591754997811E-4
56-57	1.626591253558575E-5
58-59	0.0
60-61	7.801716988306583E-4
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	3.857082741753268E-5
74-75	1.9584794522211312E-5
76-77	0.0
78-79	4.0419661174106276E-5
80-81	0.0
82-83	2.09154657529118E-5
84-85	4.251504554317966E-5
86-87	5.400033393806507E-4
88-89	6.588144765257813E-5
90-91	4.470265692476565E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	82.0
20-21	356.0
22-23	822.0
24-25	1205.0
26-27	2753.0
28-29	7306.0
30-31	17101.0
32-33	21248.0
34-35	28285.0
36-37	42131.0
38-39	51607.0
40-41	46300.0
42-43	50982.0
44-45	58348.0
46-47	57314.0
48-49	54044.0
50-51	49378.0
52-53	46435.0
54-55	58458.0
56-57	62666.0
58-59	63286.0
60-61	64938.0
62-63	65142.0
64-65	64629.0
66-67	69972.0
68-69	52590.0
70-71	44077.0
72-73	40011.0
74-75	39049.0
76-77	39147.0
78-79	41604.0
80-81	42301.0
82-83	39180.0
84-85	37186.0
86-87	37658.0
88-89	39125.0
90-91	41016.0
92-93	39519.0
94-95	41243.0
96-97	65186.0
98-99	315247.0
100-101	1745183.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.33788435243065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.84420056237592	75.77269796766245
2	6.767711536033561	11.415489457132184
3	1.804452626671096	4.5655115084287985
4	0.7246238431094646	2.4445296751671948
5	0.3257008869474737	1.3734461868430061
6	0.17153467094440958	0.8680122744325116
7	0.10178239756264275	0.6008878452325898
8	0.061758597151687	0.4166871539477865
9	0.042033363077659355	0.3190504422768627
>10	0.15401859366823945	1.9607961265219975
>50	0.0012870876349764	0.06883090219193765
>100	8.432168414896585E-4	0.1698306769509706
>500	5.261798143245469E-5	0.024229783211681535
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	5.4287195550621456E-5	0.0	0.0	0.0	0.0
3	5.4287195550621456E-5	0.0	0.0	0.0	0.0
4	5.4287195550621456E-5	0.0	0.0	0.0	0.0
5	1.0857439110124291E-4	0.0	0.0	0.0	0.0
6	1.6286158665186436E-4	0.0	0.0	0.0	0.0
7	2.7143597775310726E-4	0.0	0.0	0.0	0.0
8	2.7143597775310726E-4	0.0	0.0	0.0	0.0
9	2.7143597775310726E-4	0.0	0.0	0.0	0.0
10-11	3.257231733037287E-4	0.0	0.0	0.0	0.0
12-13	3.664385699666948E-4	0.0	0.0	0.0	0.0
14-15	5.293001566185592E-4	0.0	0.0	1.3571798887655364E-5	0.0
16-17	5.564437543938699E-4	0.0	0.0	2.7143597775310728E-5	0.0
18-19	7.87164335484011E-4	0.0	0.0	2.7143597775310728E-5	0.0
20-21	9.228823243605646E-4	0.0	0.0	5.4287195550621456E-5	0.0
22-23	0.0010993157099000845	0.0	0.0	6.785899443827681E-5	0.0
24-25	0.0013571798887655362	0.0	0.0	1.3571798887655363E-4	0.0
26-27	0.0013571798887655362	0.0	0.0	1.7643338553951973E-4	0.0
28-29	0.001710046659844576	0.0	0.0	8.143079332593218E-4	0.0
30-31	0.0019000518442717509	0.0	0.0	0.002917936760845903	0.0
32-33	0.001913623643159406	0.0	0.0	0.007220197008232653	0.0
34-35	0.0021036288275865815	0.0	0.0	0.013164644921025703	0.0
36-37	0.002470067397553276	0.0	0.0	0.020995572879202846	0.0
38-39	0.0027957905708570047	0.0	0.0	0.033210191878092675	0.0
40-41	0.0027957905708570047	0.0	0.0	0.04615768801691589	0.0
42-43	0.0029315085597335583	0.0	0.0	0.05749014008810811	0.0
44-45	0.0032029445374866655	0.0	0.0	0.06939260771258188	0.0
46-47	0.0037051010963299142	0.0	0.0	0.0819736652814384	0.0
48-49	0.003854390884094123	0.0	0.0	0.09380827391147387	0.0
50-51	0.003854390884094123	0.0	0.0	0.10545287735708217	0.0
52-53	0.003881534481869434	0.0	0.0	0.1169074756182633	0.0
54-55	0.003935821677420056	0.0	0.0	0.12822635589056786	0.0
56-57	0.004125826861847231	0.0	0.0	0.13908379500069218	0.0
58-59	0.004125826861847231	0.0	0.0	0.15036195987633377	0.0
60-61	0.004139398660734886	0.0	0.0	0.16157226575753708	0.0
62-63	0.004207257655173163	0.0	0.0	0.1716696841299527	0.0
64-65	0.004234401252948473	0.0	0.0	0.1807899329824571	0.0
66-67	0.004370119241825027	0.0	0.0	0.18961160225943308	0.0
68-69	0.004370119241825027	0.0	0.0	0.19935615386076963	0.0
70-71	0.004424406437375648	0.0	0.0	0.20764852298112707	0.0
72-73	0.004492265431813925	0.0	0.0	0.21432584803385352	0.0
74-75	0.004519409029589236	0.0	0.0	0.2197274239911403	0.0
76-77	0.004546552627364546	0.0	0.0	0.22473541778068518	0.0
78-79	0.004573696225139858	0.0	0.0	0.22909196522362252	0.0
80-81	0.00465512701846579	0.0	0.0	0.23130416844231036	0.0
82-83	0.004668698817353445	0.0	0.0	0.2323899123533228	0.0
84-85	0.004736557811791721	0.0	0.0	0.2329599279066043	0.0
86-87	0.004817988605117654	0.0	0.0	0.2332042202865821	0.0
88-89	0.00483156040400531	0.0	0.0	0.2332720792810204	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	8665	0.0	56.955807	1
TATACAC	10035	0.0	50.306156	3
TTATACA	10120	0.0	49.152508	2
GGTATCA	7015	0.0	35.76015	1
TACACAT	14330	0.0	35.255577	5
ATACACA	14980	0.0	34.24666	4
ACACATC	15485	0.0	32.09685	6
GTATCAA	11750	0.0	31.808533	1
ACATCTC	16485	0.0	29.889498	8
CACATCT	16855	0.0	29.418535	7
GTGCGCT	1480	0.0	29.259405	9
TTGTGCG	1630	0.0	26.806162	7
CATCTCC	18660	0.0	26.426502	9
AACGCAG	14220	0.0	25.73401	6
ATCAACG	14295	0.0	25.571705	3
CAACGCA	14375	0.0	25.429392	5
TCAACGC	14575	0.0	25.107212	4
TATCAAC	15020	0.0	24.67571	2
ACGCAGA	15880	0.0	22.970232	7
TGCGCGA	430	3.6561687E-10	22.889692	94-95
>>END_MODULE