FastQCFastQC Report
Fri 27 May 2016
SRR2139867_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139867_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8982671
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC142200.044.975081
TATACAC194150.035.4679873
TTATACA191900.034.3873372
GGTATCA110950.032.7588841
GTATCAA170550.027.0891171
ACACATC238400.026.7928096
TACACAT254150.026.3882625
ACATCTC248300.025.6924158
CACATCT264350.024.13257
CATCTCC272100.023.3131929
ATACACA316050.023.0630054
ATCAACG200400.022.3969023
TCAACGC201700.022.252554
AACGCAG200700.022.1845196
CAACGCA202900.022.1012785
TATCAAC216850.020.9557062
ACGCAGA230900.019.248397
CGCAGAG235150.018.917478
TATGCCG145050.018.85486842-43
CCGTCTT146150.018.68683846-47
Produced by FastQC (version 0.11.5)