FastQCFastQC Report
Fri 27 May 2016
SRR2139866_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139866_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13951609
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160350.045.7565881
GTATCAA278900.032.3559461
ATCAACG344750.025.6989633
TCAACGC344800.025.6603744
CAACGCA351050.025.1692775
TATCAAC366850.024.347582
AACGCAG364250.024.1581616
ACGCAGA432950.020.315527
GTACATG481950.020.2788851
TACATGG471950.020.2415852
CGCAGAG439200.020.0264238
ACATGGG483950.019.2758523
CATGGGG308500.017.0545654
GCAGAGT518050.016.7385069
AGAGTAC477250.016.17154910-11
GAGTACT269350.016.15724612-13
CAGAGTA511050.015.55677310-11
GTGGTAT79100.015.5523541
TGGTATC72250.015.5293912
GAGTACA348650.014.4930471
Produced by FastQC (version 0.11.5)