##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139866_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13951609 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.719505542335654 31.0 31.0 34.0 30.0 34.0 2 31.901106316841304 31.0 31.0 34.0 30.0 34.0 3 31.925149708538992 31.0 31.0 34.0 30.0 34.0 4 35.65985371293017 37.0 35.0 37.0 33.0 37.0 5 35.47090955602325 37.0 35.0 37.0 33.0 37.0 6 35.47322226418473 37.0 35.0 37.0 33.0 37.0 7 35.358862049531346 37.0 35.0 37.0 33.0 37.0 8 35.32571246800279 37.0 35.0 37.0 32.0 37.0 9 36.88162146745942 39.0 37.0 39.0 33.0 39.0 10-11 36.71985335168152 39.0 36.0 39.0 32.0 39.0 12-13 36.6800189856238 39.0 35.0 39.0 32.0 39.0 14-15 37.77655767159186 40.0 37.0 41.0 32.0 41.0 16-17 37.78984649727498 40.0 37.0 41.0 32.0 41.0 18-19 37.78120054109888 40.0 37.0 41.0 32.0 41.0 20-21 37.751949298831036 40.0 37.0 41.0 32.0 41.0 22-23 37.67035801038597 39.0 37.0 41.0 32.0 41.0 24-25 37.56955885154471 39.0 36.0 41.0 32.0 41.0 26-27 37.39928468992375 39.0 36.0 41.0 32.0 41.0 28-29 37.25178221297091 39.0 36.0 40.0 31.0 41.0 30-31 37.1201748820167 39.0 36.0 40.0 31.0 41.0 32-33 37.29293534117534 39.0 36.0 40.0 31.0 41.0 34-35 37.262296756037216 39.0 36.0 40.5 31.0 41.0 36-37 37.151561088747826 39.0 36.0 40.0 31.0 41.0 38-39 36.98347850695146 39.0 35.5 40.0 31.0 41.0 40-41 36.959738029238665 39.0 35.0 40.0 31.0 41.0 42-43 36.841834926681216 39.0 35.0 40.0 30.0 41.0 44-45 36.89743400947235 39.0 35.0 40.0 30.5 41.0 46-47 37.054865282813594 39.0 36.0 40.0 31.0 41.0 48-49 36.994342805560365 39.0 35.5 40.0 31.0 41.0 50-51 36.87819544955282 39.0 35.0 40.0 30.0 41.0 52-53 36.74101705084131 39.0 35.0 40.0 30.0 41.0 54-55 36.60794251574494 38.5 35.0 40.0 30.0 41.0 56-57 36.44307796697322 38.0 35.0 40.0 30.0 41.0 58-59 36.2936734016687 38.0 34.5 40.0 29.0 41.0 60-61 36.09823121095111 38.0 34.0 40.0 29.0 41.0 62-63 35.84858345795976 38.0 34.0 40.0 28.5 41.0 64-65 35.62769879760228 37.0 34.0 40.0 28.0 41.0 66-67 35.358471923769386 37.0 34.0 39.5 28.0 41.0 68-69 35.03914930224822 36.0 33.0 39.0 28.0 40.5 70-71 34.690836477725625 36.0 33.0 39.0 28.0 40.0 72-73 34.39774740102309 35.5 33.0 38.5 28.0 40.0 74-75 34.03369353548008 35.0 32.0 38.0 27.0 40.0 76-77 32.951015664018506 34.0 31.0 36.0 26.0 39.0 78-79 33.30475481986213 35.0 32.0 36.5 26.0 39.0 80-81 33.17187557992625 35.0 32.0 36.0 26.0 38.5 82-83 32.72699132840813 34.0 31.0 36.0 26.0 37.5 84-85 32.467490763850805 34.0 31.0 35.0 26.0 37.0 86-87 32.36627098419413 34.0 31.0 35.0 26.0 36.5 88-89 32.192307603229594 34.0 31.0 35.0 26.0 36.0 90-91 32.05196902418695 34.0 31.0 35.0 26.0 36.0 92-93 31.960330969436836 34.0 31.0 35.0 26.0 35.5 94-95 31.905566331235633 34.0 31.0 35.0 26.0 35.0 96-97 31.82307622268589 34.0 31.0 35.0 26.0 35.0 98-99 32.04527811379863 34.0 31.0 35.0 26.5 35.0 100-101 31.173489799909355 33.0 29.0 34.5 25.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 0.0 14 1.0 15 0.0 16 0.0 17 1.0 18 4.0 19 23.0 20 165.0 21 892.0 22 4031.0 23 13096.0 24 33049.0 25 68664.0 26 119863.0 27 186401.0 28 264354.0 29 348875.0 30 439632.0 31 540521.0 32 663557.0 33 823144.0 34 1048603.0 35 1360789.0 36 1869731.0 37 2530377.0 38 2419376.0 39 1181792.0 40 34667.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.014779371668126 22.46703749130566 14.787571439793892 24.73061169723232 2 14.271413650272931 23.456272719896486 39.70791377054736 22.56439985928322 3 17.059669183628813 27.707431839213935 31.633065638961877 23.599833338195378 4 11.967895602578887 19.39939687243242 39.17532379240273 29.457383732585967 5 12.105657490831344 38.88632486761921 36.456246731111804 12.551770910437643 6 28.9558716847641 39.394782350910205 18.907661474744597 12.741684489581095 7 25.335429053380153 33.21560975511857 24.173355202256598 17.27560598924468 8 24.1634925405378 37.16147721743062 21.803334654805763 16.87169558722582 9 25.524109799808752 15.92594803939818 21.457224037743604 37.092718123049465 10-11 23.557879493173832 27.27795606069207 29.73334686582364 19.430817580310457 12-13 24.24670245011534 24.991161413395453 29.7282101916779 21.033925944811312 14-15 21.45940299789078 26.368510614080424 27.11729521663057 25.05479117139822 16-17 21.05776707967931 29.36354580895135 27.968906895220254 21.609780216149087 18-19 21.107611863673302 28.623578436950382 29.49189911774166 20.776910581634656 20-21 21.992429888082636 27.8783426122022 29.2000783181763 20.929149181538858 22-23 21.651159242785003 27.935325783512997 28.991920185756438 21.421594787945562 24-25 21.364592275278248 28.044931908497496 29.17497025337392 21.41550556285034 26-27 21.372474999655644 28.16900958805677 29.095266320243162 21.363249092044427 28-29 21.288329477153727 28.18397897797393 29.08026482469711 21.447426720175233 30-31 21.547862084099595 28.123345585631498 29.029775018699848 21.299017311569056 32-33 21.36355341570104 28.216998763867533 29.00224894659647 21.41719887383495 34-35 21.45158607625543 28.291371502910472 29.01376224515718 21.243280175676915 36-37 21.412140569482563 28.198450364341472 28.96579317469455 21.42361589148141 38-39 21.226233338003585 28.216665019245358 29.068856475808087 21.48824516694297 40-41 21.416292787023497 28.111772956907107 28.992422716647198 21.479511539422198 42-43 21.410439307948316 28.189202544380247 28.81518228937044 21.585175858301 44-45 21.44179274071282 28.297456081987505 28.60537313951304 21.655378037786637 46-47 21.532781844916624 28.224886129257193 28.386324892535804 21.85600713329038 48-49 21.45214130796271 28.333883695667723 28.397520606251714 21.816454390117855 50-51 21.44662567855379 28.453263604602313 28.268542211273417 21.83156850557048 52-53 21.52200182514071 28.36897392699791 28.195082061356963 21.913942186504414 54-55 21.495385167730042 28.31272549905793 28.22137849469121 21.970510838520827 56-57 21.555028845158823 28.286375346985015 28.17780969739897 21.98078611045719 58-59 21.4926003379788 28.324964692838144 28.217224543515396 21.965210425667657 60-61 21.551190837197748 28.348011446458475 28.136698219369066 21.96409949697471 62-63 21.461929820903684 28.43771069761944 28.133638440860448 21.966721040616427 64-65 21.411617659144206 28.45156849249434 28.10282799645683 22.033985851904625 66-67 21.534369246861413 28.55451426336943 27.83475552731612 22.076360962453037 68-69 21.566621510549094 28.528189133449818 27.82129458925166 22.083894766749427 70-71 21.491876066190823 28.334435444179867 27.94202743278676 22.23166105684255 72-73 21.529422888003957 28.27049582134309 27.89321442752292 22.306866863130033 74-75 21.591129218310684 28.29087749771171 27.80665456790417 22.311338716073436 76-77 21.754406086920454 28.323753310151485 27.721159206821593 22.200681396106468 78-79 21.763934804734877 28.254729325932797 27.65605270486458 22.325283164467745 80-81 21.712342455920957 28.26253483358973 27.664780068447918 22.36034264204139 82-83 21.669516692929584 28.271007200213543 27.73196743377586 22.327508673081013 84-85 21.84077312909416 28.295824902303025 27.75118898422016 22.11221298438266 86-87 21.746052093139834 28.26348217038656 27.83132890332938 22.159136833144224 88-89 21.725561997333834 28.23456298078657 27.862348088855278 22.177526933024314 90-91 21.79235969858396 28.22679453441624 27.922197592417824 22.058648174581975 92-93 21.770393016897486 28.148929869563112 27.999048210449317 22.08162890309008 94-95 21.679519383675963 28.158938108369096 28.121591200221935 22.039951307733006 96-97 21.49153609378601 28.160111321596972 28.30298697854239 22.04536560607463 98-99 21.544064864889798 28.770354471182074 28.449420130692143 21.236160533235985 100-101 24.11599352378006 36.51664768998128 29.777877799114115 9.589480987124546 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2821.0 1 2704.0 2 3797.5 3 5338.0 4 7159.0 5 10808.5 6 13836.0 7 16288.5 8 18036.5 9 17977.0 10 16934.0 11 16171.5 12 18351.5 13 25053.5 14 39585.5 15 58842.5 16 73400.5 17 80005.0 18 81160.5 19 79362.5 20 76286.5 21 74642.5 22 76949.0 23 86097.0 24 102850.0 25 127027.5 26 158806.0 27 193913.0 28 231170.5 29 269975.0 30 306010.5 31 340608.5 32 375457.0 33 409210.5 34 440919.5 35 470623.0 36 500712.5 37 526669.0 38 547089.5 39 565694.0 40 582392.5 41 595201.5 42 603433.5 43 611786.0 44 618048.0 45 619043.5 46 616619.5 47 612111.0 48 607980.0 49 596485.5 50 577740.5 51 554811.0 52 524402.5 53 490362.0 54 453282.5 55 414457.5 56 374809.0 57 333306.0 58 291317.0 59 250379.0 60 210673.0 61 171405.5 62 135812.0 63 105877.5 64 79608.5 65 58985.0 66 43618.0 67 32520.0 68 24389.5 69 17350.5 70 12017.5 71 8488.5 72 5906.5 73 4071.0 74 2810.5 75 1939.0 76 1338.5 77 911.0 78 647.5 79 469.0 80 332.5 81 225.5 82 170.0 83 126.0 84 90.0 85 70.0 86 58.5 87 47.5 88 33.0 89 21.0 90 12.0 91 7.0 92 5.5 93 4.5 94 4.0 95 4.5 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.16763206308319E-6 2 0.0012973414034180572 3 1.433526412616638E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.16763206308319E-6 12-13 3.583816031541595E-6 14-15 0.0 16-17 0.0015768790538783018 18-19 0.0014084397003958468 20-21 3.58402655276248E-5 22-23 0.002616683847167597 24-25 1.3266120712017798E-4 26-27 0.0012913234257260896 28-29 5.563060702682759E-4 30-31 1.7615898139556235E-4 32-33 9.811337714937282E-4 34-35 0.0165973461713202 36-37 0.03246272159766359 38-39 0.007227745790970866 40-41 0.004393977163224531 42-43 0.007669598078079216 44-45 0.005358865961888826 46-47 0.0032108976707777366 48-49 0.001316932764820775 50-51 1.9464390037457827E-4 52-53 1.2466792586633853E-4 54-55 0.0045933825103278885 56-57 0.012214359494529158 58-59 0.009698599304877977 60-61 0.0082064070058784 62-63 0.001942709538777064 64-65 0.0013383142532436081 66-67 0.001467693856673824 68-69 0.0 70-71 4.61005631229886E-6 72-73 5.304801948974847E-4 74-75 6.882396335697484E-4 76-77 7.30233634356733E-5 78-79 7.395120074415752E-4 80-81 0.0024126140790121987 82-83 0.004609134040039599 84-85 0.012487614861859002 86-87 0.00817928030982876 88-89 0.07663823359778747 90-91 0.0 92-93 0.0 94-95 1.5159462384825985E-4 96-97 0.008226900582124575 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 524.0 20-21 1503.0 22-23 2916.0 24-25 5696.0 26-27 7559.0 28-29 15566.0 30-31 43047.0 32-33 58828.0 34-35 83335.0 36-37 124217.0 38-39 134867.0 40-41 143227.0 42-43 157550.0 44-45 184691.0 46-47 186381.0 48-49 173182.0 50-51 160487.0 52-53 139957.0 54-55 157001.0 56-57 166749.0 58-59 176143.0 60-61 179184.0 62-63 171418.0 64-65 185454.0 66-67 198586.0 68-69 198066.0 70-71 197322.0 72-73 189107.0 74-75 191660.0 76-77 192184.0 78-79 207261.0 80-81 211562.0 82-83 203001.0 84-85 201777.0 86-87 214552.0 88-89 232261.0 90-91 255351.0 92-93 274703.0 94-95 347032.0 96-97 422169.0 98-99 1129540.0 100-101 6425993.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.22072198610942 #Duplication Level Percentage of deduplicated Percentage of total 1 91.25080067851026 79.58960716990228 2 6.239713818448213 10.884646884635137 3 1.4174099886278702 3.708825676753379 4 0.5160978404027154 1.8005770502158687 5 0.22558384784477353 0.9837793038712899 6 0.12387660368924276 0.6482764086577739 7 0.0659548672066566 0.40268417953837915 8 0.04200405661112583 0.2930899315174246 9 0.02441136730470594 0.1916259372886102 >10 0.09080322872695192 1.2374993247027117 >50 0.002622863530539433 0.15233753110915957 >100 6.950751489369861E-4 0.09053872626064721 >500 2.576394806683428E-5 0.016511875547365138 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.433526412616638E-5 0.0 3.583816031541595E-5 0.0 3 0.0 1.433526412616638E-5 0.0 3.583816031541595E-5 0.0 4 0.0 5.0173424441582326E-5 0.0 5.0173424441582326E-5 0.0 5 0.0 5.0173424441582326E-5 0.0 5.0173424441582326E-5 0.0 6 0.0 5.0173424441582326E-5 0.0 5.0173424441582326E-5 0.0 7 0.0 5.0173424441582326E-5 0.0 5.734105650466552E-5 0.0 8 0.0 5.734105650466552E-5 0.0 5.734105650466552E-5 7.16763206308319E-6 9 0.0 1.0751448094624785E-4 0.0 7.88439526939151E-5 2.150289618924957E-5 10-11 0.0 1.0751448094624785E-4 0.0 1.1109829697778945E-4 2.150289618924957E-5 12-13 0.0 1.0751448094624785E-4 0.0 1.1826592904087263E-4 2.150289618924957E-5 14-15 0.0 1.2184974507241423E-4 0.0 1.290173771354974E-4 2.867052825233276E-5 16-17 0.0 1.2184974507241423E-4 0.0 1.7560698554553816E-4 2.867052825233276E-5 18-19 0.0 1.433526412616638E-4 0.0 2.3653185808174526E-4 3.942197634695755E-5 20-21 0.0 1.6843935348245494E-4 0.0 5.519076688574056E-4 5.0173424441582326E-5 22-23 0.0 1.9352606570324614E-4 0.0 0.001204162186597976 6.092487253620711E-5 24-25 0.0 2.042775137978709E-4 0.0 0.0025660122785837822 7.16763206308319E-5 26-27 0.0 2.078613298294125E-4 0.0 0.00640427924836483 7.52601366623735E-5 28-29 0.0 2.078613298294125E-4 0.0 0.03136914172408358 8.601158475699829E-5 30-31 0.0 2.4369949014482845E-4 0.0 0.05224128629178183 9.317921682008147E-5 32-33 0.0 2.4728330617637006E-4 0.0 0.07447169713543433 9.317921682008147E-5 34-35 0.0 2.9028909855486923E-4 0.0 0.10581575214729713 9.317921682008147E-5 36-37 0.0 3.1895962680720196E-4 0.0 0.14907957928006726 9.317921682008147E-5 38-39 0.0 3.2254344283874354E-4 0.0 0.2177777487886881 1.0751448094624785E-4 40-41 0.0 3.7988449934340905E-4 0.0 0.32264020587159514 1.0751448094624785E-4 42-43 0.0 3.870521314064923E-4 0.0 0.37722530784800523 1.0751448094624785E-4 44-45 0.0 4.300579237849914E-4 0.0 0.45894348099921667 1.1109829697778945E-4 46-47 0.0 4.6589608410040734E-4 0.0 0.5434785335512198 1.1468211300933104E-4 48-49 0.0 4.6589608410040734E-4 0.0 0.6186419071807416 1.290173771354974E-4 50-51 0.0 5.053180604473648E-4 0.0 0.694615223233392 1.433526412616638E-4 52-53 0.0 5.089018764789065E-4 0.0 0.7759642633333546 1.50520273324747E-4 54-55 0.0 5.124856925104481E-4 0.0 0.9470843112074027 1.576879053878302E-4 56-57 0.0 5.232371406050729E-4 0.0 1.1027867825137587 1.576879053878302E-4 58-59 0.0 5.232371406050729E-4 0.0 1.2319869342668648 1.6127172141937177E-4 60-61 0.0 5.268209566366144E-4 0.0 1.3077846433339695 1.6485553745091336E-4 62-63 0.0 5.375724047312392E-4 0.0 1.348873094135594 1.6485553745091336E-4 64-65 0.0 5.877458291728215E-4 0.0 1.3772461656573087 1.6485553745091336E-4 66-67 0.0 6.02081093298988E-4 0.0 1.3905385393182965 1.6485553745091336E-4 68-69 0.0 6.092487253620711E-4 0.0 1.402863282650768 1.6485553745091336E-4 70-71 0.0 6.128325413936127E-4 0.0 1.4145071009372467 1.6485553745091336E-4 72-73 0.0 6.164163574251543E-4 0.0 1.4229075657151802 1.8994224967170455E-4 74-75 0.0 6.20000173456696E-4 0.0 1.4308277991448872 1.9352606570324614E-4 76-77 0.0 6.415030696459456E-4 0.0 1.4381567029293896 1.9352606570324614E-4 78-79 0.0 6.450868856774871E-4 0.0 1.4451953176153374 1.9352606570324614E-4 80-81 0.0 6.665897818667367E-4 0.0 1.4494600586928719 1.9352606570324614E-4 82-83 0.0 6.665897818667367E-4 0.0 1.4526890769372909 1.9352606570324614E-4 84-85 0.0 6.845088620244446E-4 0.0 1.4554342800174518 1.9352606570324614E-4 86-87 0.0 7.024279421821527E-4 0.0 1.4559575171580568 2.006936977663293E-4 88-89 0.0 7.095955742452358E-4 0.0 1.4560614478229714 2.078613298294125E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16035 0.0 45.756588 1 GTATCAA 27890 0.0 32.355946 1 ATCAACG 34475 0.0 25.698963 3 TCAACGC 34480 0.0 25.660374 4 CAACGCA 35105 0.0 25.169277 5 TATCAAC 36685 0.0 24.34758 2 AACGCAG 36425 0.0 24.158161 6 ACGCAGA 43295 0.0 20.31552 7 GTACATG 48195 0.0 20.278885 1 TACATGG 47195 0.0 20.241585 2 CGCAGAG 43920 0.0 20.026423 8 ACATGGG 48395 0.0 19.275852 3 CATGGGG 30850 0.0 17.054565 4 GCAGAGT 51805 0.0 16.738506 9 AGAGTAC 47725 0.0 16.171549 10-11 GAGTACT 26935 0.0 16.157246 12-13 CAGAGTA 51105 0.0 15.556773 10-11 GTGGTAT 7910 0.0 15.552354 1 TGGTATC 7225 0.0 15.529391 2 GAGTACA 34865 0.0 14.493047 1 >>END_MODULE