FastQCFastQC Report
Fri 27 May 2016
SRR2139865_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139865_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13118654
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139650.040.2109681
GTATCAA223950.031.0761491
ATCAACG298450.022.7308433
TCAACGC299600.022.6569044
CAACGCA305200.022.2019795
TATCAAC313400.021.9392152
CTTATAC272150.021.5276341
AACGCAG327850.020.8262656
TATACAC311400.020.5816463
TTATACA327500.018.7782842
ACGCAGA364400.018.595077
TACATGG354600.018.062992
CGCAGAG383300.017.6677688
GTACATG367650.017.6496371
ACATGGG358250.017.467053
TACACAT383100.016.5006085
AGAGTAC398200.016.12587210-11
ATACACA434950.015.4321924
ACACATC392950.015.1836836
CATGGGG260800.015.1700474
Produced by FastQC (version 0.11.5)