##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139865_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13118654
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.465109377837084	31.0	31.0	34.0	30.0	34.0
2	31.68548503527877	31.0	31.0	34.0	30.0	34.0
3	31.694098266483742	31.0	31.0	34.0	30.0	34.0
4	35.47591612676118	37.0	35.0	37.0	33.0	37.0
5	35.253019783889414	37.0	35.0	37.0	33.0	37.0
6	35.25522305870709	37.0	35.0	37.0	32.0	37.0
7	35.13382394260875	37.0	35.0	37.0	32.0	37.0
8	35.09967966225803	37.0	35.0	37.0	32.0	37.0
9	36.62792356593901	38.0	35.0	39.0	32.0	39.0
10-11	36.43135092212967	38.0	35.0	39.0	32.0	39.0
12-13	36.382533680665716	38.0	35.0	39.0	32.0	39.0
14-15	37.416140443981526	39.0	36.0	40.0	32.0	41.0
16-17	37.41081299194262	39.0	36.0	40.0	32.0	41.0
18-19	37.38266277165325	39.0	36.0	40.0	32.0	41.0
20-21	37.344331521587	39.0	36.0	40.0	32.0	41.0
22-23	37.2399255739359	39.0	36.0	40.0	31.0	41.0
24-25	37.108988937842724	39.0	36.0	40.0	31.0	41.0
26-27	36.90124565572639	39.0	36.0	40.0	31.0	41.0
28-29	36.729412705013516	38.5	35.5	40.0	30.0	41.0
30-31	36.5874989723825	38.0	35.0	40.0	30.0	41.0
32-33	36.777378536247035	38.5	35.0	40.0	30.0	41.0
34-35	36.725232572039914	38.0	35.0	40.0	30.0	41.0
36-37	36.60042559482139	38.0	35.0	40.0	30.0	41.0
38-39	36.377040977916344	38.0	35.0	40.0	30.0	41.0
40-41	36.345046292342964	38.0	35.0	40.0	30.0	41.0
42-43	36.20295189585069	38.0	35.0	40.0	30.0	41.0
44-45	36.264373484711186	38.0	35.0	40.0	30.0	41.0
46-47	36.43631086128179	38.0	35.0	40.0	30.0	41.0
48-49	36.35975010752388	38.0	35.0	40.0	30.0	41.0
50-51	36.20112354832732	38.0	34.5	40.0	29.0	41.0
52-53	36.04837839365955	38.0	34.0	40.0	29.0	41.0
54-55	35.877702110555106	38.0	34.0	40.0	28.0	41.0
56-57	35.664885869298274	38.0	34.0	40.0	28.0	41.0
58-59	35.4802878530582	37.5	34.0	40.0	28.0	41.0
60-61	35.24069136525091	37.0	33.0	40.0	28.0	41.0
62-63	34.964430424450526	37.0	33.0	39.0	27.5	41.0
64-65	34.718333663337845	36.0	33.0	39.0	27.0	40.0
66-67	34.426597002067034	36.0	32.0	39.0	27.0	40.0
68-69	34.0817338970232	35.0	32.0	38.5	26.0	40.0
70-71	33.71877105545488	35.0	32.0	38.0	26.0	40.0
72-73	33.42363159918804	35.0	31.0	37.0	26.0	39.5
74-75	33.063222577132336	34.5	31.0	37.0	26.0	39.0
76-77	32.06406125539512	34.0	30.0	36.0	25.5	38.0
78-79	32.43487368984799	34.0	31.0	36.0	26.0	38.0
80-81	32.32804814919205	34.0	31.0	35.5	26.0	37.0
82-83	31.894325972276757	34.0	30.5	35.0	25.0	37.0
84-85	31.642064532451517	34.0	30.0	35.0	25.0	36.0
86-87	31.55511834120032	34.0	30.0	35.0	25.0	36.0
88-89	31.40202211173078	34.0	30.0	35.0	25.0	35.5
90-91	31.296313998863347	34.0	30.0	35.0	25.0	35.0
92-93	31.227659260828	33.0	30.0	35.0	25.0	35.0
94-95	31.19864510901131	33.0	30.0	35.0	25.0	35.0
96-97	31.149239539281638	33.0	30.0	34.0	25.0	35.0
98-99	31.45300602220297	33.0	30.0	34.0	25.0	35.0
100-101	30.617306590644098	32.0	28.0	34.0	22.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	33.0
20	236.0
21	1315.0
22	5885.0
23	18386.0
24	44834.0
25	89785.0
26	152516.0
27	230517.0
28	319017.0
29	410685.0
30	507132.0
31	613793.0
32	741267.0
33	911706.0
34	1151395.0
35	1481456.0
36	1943153.0
37	2222604.0
38	1616064.0
39	649987.0
40	6883.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.90100020932026	23.47743144990332	15.475619678665204	25.145948662111216
2	14.931632698486846	23.53978548852038	38.23700969436261	23.291572118630167
3	17.2534558235514	27.67031035884553	31.938340010975214	23.13789380662786
4	12.693367779956693	19.84835486933339	37.52830892559557	29.929968425114346
5	12.427105707643483	38.601025684494765	36.28151180753757	12.69035680032418
6	29.882219624055946	39.17410276999455	17.80597308229945	13.137704523650061
7	25.541919163353192	32.88902199875079	23.475419048326145	18.093639789569878
8	24.903789672324613	36.48439085290305	21.21302231158776	17.398797163184575
9	25.366954567137757	15.467806377087163	20.5213736104329	38.643865445342186
10-11	24.10711876386099	26.729582928248586	29.142669667177746	20.020628640712683
12-13	24.78610229372617	24.07289650294916	28.92175523495017	22.219245968374498
14-15	22.03485967386593	25.54472432918804	25.978122450672146	26.442293546273877
16-17	22.04068025088932	28.65859041011997	26.749051374498855	22.551677964491855
18-19	22.058574890642884	27.607590905293357	28.25546663609404	22.07836756796972
20-21	22.7401286823548	26.929646424262376	27.948134612040636	22.382090281342187
22-23	22.341410755073017	27.216014607921444	27.89527487297824	22.547299764027294
24-25	22.168437921939685	27.488541774159632	28.11081307355482	22.232207230345864
26-27	22.185174992265118	27.556657900147762	28.135432052890465	22.12273505469666
28-29	21.81526954020544	27.103764890532762	28.273557645426312	22.80740792383549
30-31	22.06097663206711	27.11614424671084	28.245043121712786	22.57783599950926
32-33	22.058215456440717	27.482939189636298	28.137621498299335	22.321223855623646
34-35	22.002938569200463	27.782475291239734	28.388587458151516	21.825998681408294
36-37	22.24936784627976	27.349227871808278	27.915702754640982	22.48570152727098
38-39	22.29102134481488	27.266680697812156	28.12551198684932	22.316785970523643
40-41	22.27171259117342	27.124226965026942	28.423304062213084	22.180756381586555
42-43	22.537803051382266	27.15804607196751	28.11053945384941	22.193611422800807
44-45	22.24906225029721	27.584129319764877	27.78626616381965	22.380542266118265
46-47	22.747804407745747	27.086812414855537	27.3820863052627	22.783296872136013
48-49	22.534249579095608	27.023545129592996	27.629513024646187	22.812692266665206
50-51	22.155496684064676	27.34526335790578	27.678475342355192	22.820764615674353
52-53	22.291992317467827	27.546517798701807	27.705694512902017	22.455795370928346
54-55	22.358895722037424	27.430183612001596	27.427129693542085	22.783790972418895
56-57	22.691824111604213	27.593304243800958	27.19184282001079	22.523028824584035
58-59	22.23812009739282	27.787982165066605	27.709422388577604	22.26447534896297
60-61	22.548041381001738	28.131576541958985	26.943809583363	22.376572493676278
62-63	22.299847000909327	28.28064792874635	26.93707187064342	22.482433199700907
64-65	22.206045147030874	27.998291157539356	27.136449117145574	22.659214578284196
66-67	22.354966668936243	27.970930130339184	26.91236171297592	22.761741487748658
68-69	22.42261160611445	27.823089993457117	27.008543033378125	22.745755367050307
70-71	22.446815837218363	27.37571204036357	27.120799649688276	23.05667247272979
72-73	22.445259893328224	27.448481654237668	27.089217177294415	23.01704127513969
74-75	22.30478023503968	27.604526922492962	27.09689202348941	22.993800818977945
76-77	22.486730993900455	27.5853990601112	26.98925447545709	22.938615470531257
78-79	22.529573370088084	27.623035306009097	26.851201550810522	22.996189773092297
80-81	22.482533936454765	27.510226540260913	26.905695082588473	23.101544440695854
82-83	22.602977297448057	27.39207399617159	27.015833302658766	22.989115403721584
84-85	22.696645985498552	27.440341078975354	26.88427557438615	22.97873736113994
86-87	22.436960046463227	27.594068957686073	27.076622210763606	22.89234878508709
88-89	22.485441206237784	27.332771661150456	27.209613789669092	22.972173342942664
90-91	22.416711317215558	27.26831648243529	27.339767599268278	22.975204601080875
92-93	22.368203904001383	27.30910520755848	27.422919508001048	22.899771380439084
94-95	22.061322851246565	27.43425150729566	27.507910307440397	22.996515334017378
96-97	21.891666506269306	27.456450721606807	27.689103607490082	22.962779164633805
98-99	22.000968512931802	28.120186020185667	27.738439422319033	22.1404060445635
100-101	25.0121738467553	36.11256906539779	28.776944325833643	10.098312762013272
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2105.0
1	2144.5
2	3505.0
3	5041.5
4	6489.5
5	9361.5
6	11636.0
7	13636.0
8	15097.5
9	15026.5
10	14118.0
11	13434.0
12	15459.5
13	21452.0
14	34704.5
15	52467.5
16	65115.0
17	69354.5
18	68941.5
19	66805.5
20	63558.5
21	60893.5
22	61088.0
23	67625.5
24	81169.0
25	101314.5
26	128887.0
27	157817.5
28	183765.5
29	209908.5
30	232779.0
31	254926.0
32	283616.0
33	313165.5
34	344159.5
35	379608.0
36	418818.5
37	448496.5
38	457823.5
39	463637.0
40	480877.0
41	495522.0
42	502582.5
43	516004.0
44	532367.0
45	552016.0
46	576715.0
47	606823.0
48	624222.0
49	628641.0
50	629768.0
51	615172.0
52	583405.0
53	546895.5
54	510933.5
55	470730.0
56	432875.5
57	399603.5
58	362175.5
59	313164.0
60	259017.0
61	205916.0
62	158964.0
63	121704.0
64	91695.0
65	67577.0
66	49225.5
67	36562.5
68	27466.0
69	20309.5
70	15211.5
71	11488.5
72	8485.5
73	6075.5
74	4341.5
75	3119.5
76	2208.5
77	1484.0
78	1033.0
79	756.0
80	562.5
81	401.0
82	282.5
83	192.0
84	131.5
85	100.0
86	79.5
87	62.0
88	44.0
89	27.0
90	14.0
91	9.0
92	8.5
93	6.5
94	4.0
95	2.5
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	7.775187911808636E-4
3	7.622733246871211E-6
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0015245466493742422
18-19	0.001265373718980621
20-21	2.286981055411264E-5
22-23	0.002344574979450658
24-25	1.3728572843921914E-4
26-27	0.0012514539090097622
28-29	4.925274235642301E-4
30-31	1.3385167330080177E-4
32-33	8.631075385193387E-4
34-35	0.015610132547823655
36-37	0.02901025372709845
38-39	0.004905444237264836
40-41	0.0030013739974226097
42-43	0.0069756156739051315
44-45	0.004874178946171821
46-47	0.0027301150311660844
48-49	0.0011368096002823934
50-51	1.3452052177819986E-4
52-53	9.36332736222598E-5
54-55	0.004165043936260269
56-57	0.010734325786437966
58-59	0.009160029713290182
60-61	0.007407542412014907
62-63	0.0016480367360701303
64-65	0.0010420802276538746
66-67	0.0012340362638947003
68-69	0.0
70-71	4.94399491994634E-6
72-73	1.8123788191919035E-4
74-75	2.614210633716951E-4
76-77	4.1769105397654416E-5
78-79	2.1290598843334992E-4
80-81	0.0020008195748443263
82-83	0.003664132595561996
84-85	0.010514935129670748
86-87	0.006817306651992783
88-89	0.07014585157934972
90-91	0.0
92-93	1.905692003455909E-5
94-95	5.948217200340714E-5
96-97	0.006401537038330981
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	555.0
20-21	1964.0
22-23	3575.0
24-25	5918.0
26-27	8289.0
28-29	15556.0
30-31	36913.0
32-33	50918.0
34-35	72139.0
36-37	109487.0
38-39	121630.0
40-41	128614.0
42-43	141717.0
44-45	162964.0
46-47	166715.0
48-49	159215.0
50-51	150051.0
52-53	134999.0
54-55	160784.0
56-57	175699.0
58-59	177851.0
60-61	197140.0
62-63	188538.0
64-65	197945.0
66-67	204621.0
68-69	185082.0
70-71	183983.0
72-73	177203.0
74-75	178586.0
76-77	178327.0
78-79	195087.0
80-81	204174.0
82-83	196952.0
84-85	197845.0
86-87	211317.0
88-89	234101.0
90-91	262501.0
92-93	289505.0
94-95	376945.0
96-97	450853.0
98-99	1104701.0
100-101	5717695.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.11336943499667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.60510589169974	74.47382269238837
2	6.727571540696618	11.183022777245757
3	1.8524133009686257	4.618809330891213
4	0.7419517995834133	2.4666445608694736
5	0.39322994351302193	1.634133278405033
6	0.22361125143045998	1.1151050729977052
7	0.12960931486251423	0.7540586807869464
8	0.08243676854052491	0.5481278078988768
9	0.05656151382002167	0.42309161945335183
>10	0.18312054372486836	2.29049169254063
>50	0.0031405265573477424	0.17221069596496208
>100	0.0010982938784885978	0.16684698035811477
>500	6.638370229365203E-5	0.02924178459140741
>1k	8.292702216205029E-5	0.12439302560814307
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.5245466493742422E-5	0.0
3	0.0	0.0	0.0	1.5245466493742422E-5	0.0
4	0.0	0.0	0.0	2.286819974061363E-5	0.0
5	0.0	0.0	0.0	2.286819974061363E-5	0.0
6	0.0	0.0	0.0	3.0490932987484844E-5	0.0
7	0.0	0.0	0.0	3.0490932987484844E-5	0.0
8	0.0	0.0	0.0	6.098186597496969E-5	0.0
9	0.0	0.0	0.0	1.5245466493742421E-4	0.0
10-11	0.0	0.0	0.0	1.9437969779521587E-4	0.0
12-13	0.0	0.0	0.0	2.4392746389987875E-4	0.0
14-15	0.0	0.0	0.0	2.6298429701705674E-4	0.0
16-17	0.0	0.0	0.0	3.697025624732537E-4	0.0
18-19	0.0	0.0	0.0	4.6879809468257946E-4	0.0
20-21	0.0	0.0	0.0	5.755163601387764E-4	0.0
22-23	0.0	0.0	0.0	8.30877923908962E-4	0.0
24-25	0.0	0.0	0.0	0.0016122080817132611	0.0
26-27	0.0	0.0	0.0	0.004020991787724564	0.0
28-29	0.0	0.0	0.0	0.02174384658670013	0.0
30-31	0.0	0.0	0.0	0.03387542654909566	0.0
32-33	0.0	0.0	0.0	0.041570575761812154	0.0
34-35	0.0	0.0	0.0	0.05248632977133172	0.0
36-37	0.0	3.8113666234356055E-6	0.0	0.06930207931392962	0.0
38-39	0.0	7.622733246871211E-6	0.0	0.10201885040950084	0.0
40-41	0.0	7.622733246871211E-6	0.0	0.16935045317911424	0.0
42-43	0.0	7.622733246871211E-6	0.0	0.197066711264738	0.0
44-45	0.0	7.622733246871211E-6	0.0	0.23914419878746707	0.0
46-47	0.0	7.622733246871211E-6	0.0	0.29117697593061	0.0
48-49	0.0	7.622733246871211E-6	0.0	0.3346646691040102	0.0
50-51	0.0	7.622733246871211E-6	0.0	0.38244396109539897	0.0
52-53	0.0	7.622733246871211E-6	0.0	0.43755251110365434	0.0
54-55	0.0	7.622733246871211E-6	3.8113666234356055E-6	0.5776964618473815	0.0
56-57	0.0	7.622733246871211E-6	7.622733246871211E-6	0.7046911977402559	0.0
58-59	0.0	7.622733246871211E-6	7.622733246871211E-6	0.8390266257498673	0.0
60-61	0.0	7.622733246871211E-6	7.622733246871211E-6	0.9154178469833871	0.0
62-63	0.0	7.622733246871211E-6	7.622733246871211E-6	0.9489197596033861	0.0
64-65	0.0	7.622733246871211E-6	7.622733246871211E-6	0.9821167628935104	0.0
66-67	0.0	7.622733246871211E-6	7.622733246871211E-6	0.9960892329350253	0.0
68-69	0.0	7.622733246871211E-6	7.622733246871211E-6	1.0064752069838874	0.0
70-71	0.0	7.622733246871211E-6	7.622733246871211E-6	1.0170669948304147	0.0
72-73	0.0	7.622733246871211E-6	7.622733246871211E-6	1.0254901150682074	0.0
74-75	0.0	7.622733246871211E-6	7.622733246871211E-6	1.0338903671062596	0.0
76-77	0.0	7.622733246871211E-6	7.622733246871211E-6	1.0410709818248123	0.0
78-79	0.0	7.622733246871211E-6	7.622733246871211E-6	1.0480534054789463	0.0
80-81	0.0	7.622733246871211E-6	7.622733246871211E-6	1.0527604432588893	0.0
82-83	0.0	7.622733246871211E-6	7.622733246871211E-6	1.0560267844551734	0.0
84-85	0.0	7.622733246871211E-6	7.622733246871211E-6	1.0593045597513282	0.0
86-87	0.0	7.622733246871211E-6	7.622733246871211E-6	1.0598381510786092	0.0
88-89	0.0	7.622733246871211E-6	7.622733246871211E-6	1.060002039843417	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13965	0.0	40.210968	1
GTATCAA	22395	0.0	31.076149	1
ATCAACG	29845	0.0	22.730843	3
TCAACGC	29960	0.0	22.656904	4
CAACGCA	30520	0.0	22.201979	5
TATCAAC	31340	0.0	21.939215	2
CTTATAC	27215	0.0	21.527634	1
AACGCAG	32785	0.0	20.826265	6
TATACAC	31140	0.0	20.581646	3
TTATACA	32750	0.0	18.778284	2
ACGCAGA	36440	0.0	18.59507	7
TACATGG	35460	0.0	18.06299	2
CGCAGAG	38330	0.0	17.667768	8
GTACATG	36765	0.0	17.649637	1
ACATGGG	35825	0.0	17.46705	3
TACACAT	38310	0.0	16.500608	5
AGAGTAC	39820	0.0	16.125872	10-11
ATACACA	43495	0.0	15.432192	4
ACACATC	39295	0.0	15.183683	6
CATGGGG	26080	0.0	15.170047	4
>>END_MODULE