FastQCFastQC Report
Fri 27 May 2016
SRR2139864_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139864_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9966268
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC129450.039.4313931
GGTATCA113600.035.9743231
TATACAC179400.031.4983963
GTATCAA184250.028.9072021
TTATACA189750.028.3406142
ATCAACG228350.022.8733983
ACACATC227750.022.8641096
TACACAT242750.022.6910615
CAACGCA230850.022.5227685
ACATCTC232400.022.4066318
TCAACGC233200.022.3297634
AACGCAG240250.021.690996
TATCAAC246350.021.4755942
CACATCT246200.021.0220187
CATCTCC259500.019.9751139
ATACACA301850.019.7963434
ACGCAGA276850.018.7661957
TACATGG252050.018.6489892
CGCAGAG278900.018.6140588
GTACATG261900.018.4314481
Produced by FastQC (version 0.11.5)