##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139864_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9966268
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59431745162783	31.0	31.0	34.0	30.0	34.0
2	31.801751668728958	31.0	31.0	34.0	30.0	34.0
3	31.823074193870767	31.0	31.0	34.0	30.0	34.0
4	35.57910413406503	37.0	35.0	37.0	33.0	37.0
5	35.38116645067141	37.0	35.0	37.0	33.0	37.0
6	35.3788686998985	37.0	35.0	37.0	33.0	37.0
7	35.260625642417004	37.0	35.0	37.0	32.0	37.0
8	35.22765241713348	37.0	35.0	37.0	32.0	37.0
9	36.76729664504306	39.0	37.0	39.0	32.0	39.0
10-11	36.598041112279944	38.5	35.0	39.0	32.0	39.0
12-13	36.553146674361955	38.0	35.0	39.0	32.0	39.0
14-15	37.62037063422336	39.0	36.0	41.0	32.0	41.0
16-17	37.62815464123582	39.0	36.0	41.0	32.0	41.0
18-19	37.60804866977288	39.0	36.5	41.0	32.0	41.0
20-21	37.58031118517457	39.0	36.0	41.0	32.0	41.0
22-23	37.48470921688453	39.0	36.0	41.0	32.0	41.0
24-25	37.3672365131594	39.0	36.0	40.5	32.0	41.0
26-27	37.184036592115476	39.0	36.0	40.0	31.0	41.0
28-29	37.03281437240026	39.0	36.0	40.0	31.0	41.0
30-31	36.89114535362999	39.0	36.0	40.0	30.5	41.0
32-33	37.062227316286624	39.0	36.0	40.0	31.0	41.0
34-35	37.026825010012075	39.0	36.0	40.0	31.0	41.0
36-37	36.90179969572115	39.0	35.5	40.0	30.0	41.0
38-39	36.703644664371865	38.0	35.0	40.0	30.0	41.0
40-41	36.70668699903743	38.0	35.0	40.0	30.0	41.0
42-43	36.58433466411019	38.0	35.0	40.0	30.0	41.0
44-45	36.65222072024561	38.5	35.0	40.0	30.0	41.0
46-47	36.825389329073744	39.0	35.0	40.0	30.0	41.0
48-49	36.77481872165347	39.0	35.0	40.0	30.0	41.0
50-51	36.651846118855346	39.0	35.0	40.0	30.0	41.0
52-53	36.53037060620362	38.5	35.0	40.0	30.0	41.0
54-55	36.39273426118945	38.0	35.0	40.0	30.0	41.0
56-57	36.22531250983668	38.0	34.5	40.0	29.0	41.0
58-59	36.0852928688341	38.0	34.0	40.0	29.0	41.0
60-61	35.87537715627147	38.0	34.0	40.0	28.5	41.0
62-63	35.639112273211964	37.5	34.0	40.0	28.0	41.0
64-65	35.4313483806108	37.0	34.0	40.0	28.0	41.0
66-67	35.167679143450485	37.0	33.0	39.0	28.0	41.0
68-69	34.85089640269477	36.0	33.0	39.0	28.0	40.0
70-71	34.5144479330355	36.0	33.0	39.0	28.0	40.0
72-73	34.22018842168707	35.0	32.0	38.0	27.0	40.0
74-75	33.85804554355285	35.0	32.0	37.5	26.0	39.5
76-77	32.8016533149675	34.0	31.0	36.0	26.0	39.0
78-79	33.159718400300655	34.5	31.0	36.0	26.0	39.0
80-81	33.02242489810313	34.0	31.0	36.0	26.0	38.5
82-83	32.549861184778585	34.0	31.0	36.0	26.0	37.5
84-85	32.2699163758229	34.0	31.0	35.0	26.0	37.0
86-87	32.15402252774228	34.0	31.0	35.0	26.0	36.5
88-89	31.97089524893934	34.0	31.0	35.0	26.0	36.0
90-91	31.82303959271806	34.0	31.0	35.0	26.0	36.0
92-93	31.719436705041403	34.0	30.5	35.0	25.0	35.0
94-95	31.661097188623	34.0	30.0	35.0	25.0	35.0
96-97	31.580275024945266	34.0	30.0	35.0	25.0	35.0
98-99	31.82519661190009	34.0	31.0	35.0	26.0	35.0
100-101	30.991029165014645	32.5	29.0	34.5	23.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	25.0
20	140.0
21	822.0
22	3594.0
23	11201.0
24	27125.0
25	54806.0
26	94932.0
27	145944.0
28	204699.0
29	268311.0
30	337905.0
31	413818.0
32	503553.0
33	623519.0
34	787392.0
35	1016518.0
36	1379417.0
37	1776691.0
38	1576908.0
39	724580.0
40	14364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.85011962351404	23.89417984746146	15.293638501392898	24.962062027631607
2	13.821684286615335	23.92888097589384	40.18111879733892	22.068315940151912
3	16.361811663101975	28.523565691791553	32.56040275055818	22.554219894548293
4	11.692069689476542	20.579418494465532	39.35476148142916	28.373750334628774
5	11.586262781614943	39.227682819687374	37.23039557033786	11.955658828359823
6	27.821096121436828	40.697370369731175	18.90862256563841	12.57291094319358
7	24.173070601753835	33.56920564447996	24.635249624031786	17.62247412973442
8	23.412304385151995	37.91044952834903	22.16312063853792	16.51412544796106
9	24.628055356327966	16.066615908783508	21.64525377001702	37.660074964871505
10-11	23.060076249203814	27.56214763640713	30.22061016219913	19.157165952189928
12-13	23.879249484360646	24.950202021458782	29.94239669252322	21.22815180165735
14-15	20.975268776637353	26.500842642401345	27.112811937226656	25.41107664373465
16-17	20.914055087392015	29.570656728470674	27.922353596100713	21.5929345880366
18-19	20.808066486837458	28.86955137920734	29.408773100633013	20.913609033322185
20-21	21.62655086195024	28.13864455600011	29.018508869612074	21.216295712437578
22-23	21.26072240517494	28.546176948439715	28.793409005234594	21.39969164115075
24-25	21.12919626931578	28.61603915525821	29.1460331990056	21.10873137642041
26-27	21.314892332828148	28.72885876358593	29.09242753723087	20.863821366355054
28-29	21.12396432835538	28.553857991700905	29.196283165707914	21.1258945142358
30-31	21.51684811853171	28.64942855063702	28.915843886515386	20.917879444315886
32-33	21.314120161374312	28.408191489216556	29.05957475644252	21.218113592966613
34-35	20.97570821934329	28.84861149019633	29.468825653948443	20.706854636511938
36-37	21.350885692635398	28.540655990510448	29.043556070479852	21.0649022463743
38-39	20.968161970716356	28.45122848311635	29.255107286518513	21.32550225964879
40-41	21.19556945485456	28.346163555902255	29.280356223056074	21.17791076618711
42-43	21.405640192811486	28.355019915278522	29.044745395821607	21.19459449608839
44-45	21.141642452136363	28.85143222966025	28.774354615467697	21.232570702735693
46-47	21.572307864793064	28.300497545083577	28.3507539658139	21.776440624309462
48-49	21.469875812006897	28.314289116351272	28.474992613107652	21.740842458534175
50-51	21.11254143493799	28.455940266814107	28.682941269159095	21.748577029088807
52-53	21.262694181094442	28.521487809341366	28.84243077901509	21.373387230549103
54-55	21.269588580541807	28.526967478681907	28.460916229050078	21.742527711726208
56-57	21.671548335056045	28.815869244461588	28.0234369125125	21.489145507969862
58-59	21.205594080410066	29.137365327440946	28.58072053121748	21.076320060931504
60-61	21.527446430563074	29.464558013796736	27.82111209948547	21.186883456154714
62-63	21.275881432944555	29.548526334214298	27.82826194204091	21.347330290800237
64-65	21.249850874299987	29.24576078443819	28.051720242570276	21.452668098691547
66-67	21.467431238462936	29.035091874067113	27.944149732839378	21.553327154630573
68-69	21.507134087463893	28.83265660108048	28.113254906730223	21.546954404725405
70-71	21.44111041768032	28.56309202539751	28.228650642369168	21.767146914553003
72-73	21.497816631697777	28.615505297286063	28.30111676758668	21.585561303429476
74-75	21.397060914363685	28.73243695666887	28.183049545595594	21.68745258337185
76-77	21.371201787048737	28.76479903238451	28.13966569395192	21.724333486614835
78-79	21.44011569806617	28.795162138119352	28.06056772615819	21.704154437656285
80-81	21.392381531522105	28.708996126710513	28.13770616574972	21.760916176017666
82-83	21.394615707283364	28.633198570394562	28.295343588114104	21.67684213420797
84-85	21.663691581124393	28.625977786332385	28.088757555872473	21.621573076670746
86-87	21.313156828723738	28.7537284816595	28.189425733888807	21.743688955727954
88-89	21.476951615268316	28.628751218674374	28.226527875001068	21.667769291056242
90-91	21.466541996741697	28.616683609192687	28.316678526025612	21.60009586804
92-93	21.401933862470468	28.65718319013738	28.26269265983371	21.678190287558436
94-95	21.0928370974325	28.790363812908204	28.404622670944523	21.712176418714773
96-97	20.968374553090396	28.811898137142762	28.56761107468896	21.65211623507789
98-99	21.006630094405978	29.471347168926588	28.670073545928236	20.851949190739198
100-101	23.392131387636454	37.38520400264721	29.892842795739643	9.329821813976695
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2203.0
1	2203.5
2	3570.5
3	5111.0
4	6592.0
5	9781.0
6	12071.0
7	13345.0
8	13769.5
9	12769.0
10	11448.0
11	10459.5
12	11995.0
13	17301.5
14	28678.5
15	43586.0
16	54141.0
17	57716.5
18	57547.5
19	56485.0
20	55125.0
21	54860.5
22	57180.5
23	64739.0
24	78419.5
25	98471.0
26	125017.0
27	151692.0
28	178397.0
29	206910.0
30	231162.0
31	254360.5
32	281200.5
33	307141.0
34	334706.0
35	361107.0
36	385278.5
37	406742.5
38	418069.0
39	424792.0
40	433475.5
41	438718.5
42	440641.0
43	444474.5
44	448727.0
45	454183.5
46	467126.0
47	483696.5
48	477169.0
49	452536.5
50	428852.0
51	400940.0
52	368598.5
53	334494.0
54	300447.0
55	268004.0
56	238736.0
57	208450.5
58	177085.5
59	150338.5
60	125142.5
61	98946.0
62	75592.0
63	57414.0
64	43114.5
65	32005.5
66	23618.0
67	17759.5
68	13502.5
69	9900.0
70	7261.5
71	5380.5
72	3752.0
73	2620.0
74	1937.0
75	1364.5
76	928.0
77	652.5
78	463.0
79	332.5
80	250.0
81	179.5
82	130.0
83	87.0
84	57.5
85	37.5
86	24.0
87	19.0
88	13.5
89	7.5
90	4.5
91	4.0
92	3.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0010234523093298313
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.001404738463786043
18-19	0.0014549076946355447
20-21	1.5051734316017584E-5
22-23	0.0026846692389645163
24-25	1.505876532580743E-4
26-27	0.001084788418798841
28-29	4.2725286161400446E-4
30-31	1.3093625313120202E-4
32-33	0.0011066480237742356
34-35	0.01656162322591481
36-37	0.03002936681899132
38-39	0.005685269667676624
40-41	0.003519082590832142
42-43	0.0074051197899608024
44-45	0.005233853721815858
46-47	0.003036369019758506
48-49	0.001433257076706816
50-51	2.0129614588334285E-4
52-53	5.1005372565910276E-5
54-55	0.004453792619921958
56-57	0.011751288102880338
58-59	0.009843164782889944
60-61	0.007990987713282152
62-63	0.001976271444392154
64-65	0.0014175365072101175
66-67	0.0013313049707343325
68-69	0.0
70-71	0.0
72-73	2.976450593490719E-4
74-75	3.442523998006916E-4
76-77	1.4021785227053718E-5
78-79	3.7152190303807036E-4
80-81	0.00231185746589208
82-83	0.004225058036798094
84-85	0.011574779582636734
86-87	0.007483437449314685
88-89	0.06935460930462523
90-91	0.0
92-93	0.0
94-95	7.8658409165138E-5
96-97	0.007046686174410889
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	396.0
20-21	1359.0
22-23	2622.0
24-25	4546.0
26-27	7241.0
28-29	14381.0
30-31	31365.0
32-33	42739.0
34-35	60318.0
36-37	91394.0
38-39	107491.0
40-41	107812.0
42-43	118638.0
44-45	135588.0
46-47	137238.0
48-49	130267.0
50-51	120840.0
52-53	119305.0
54-55	132355.0
56-57	142002.0
58-59	147865.0
60-61	153298.0
62-63	148616.0
64-65	153546.0
66-67	157878.0
68-69	137453.0
70-71	136187.0
72-73	129103.0
74-75	130473.0
76-77	131004.0
78-79	140554.0
80-81	146933.0
82-83	140432.0
84-85	141044.0
86-87	149533.0
88-89	162827.0
90-91	178361.0
92-93	196216.0
94-95	250885.0
96-97	309409.0
98-99	791858.0
100-101	4424896.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.94049867554934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.614314415888	75.2227024053711
2	6.724408555529325	11.289004148985239
3	1.886673161200156	4.751048579667485
4	0.7968517893565252	2.675525462763621
5	0.37894125642046594	1.5904259016336562
6	0.2060217776664308	1.0376142453206014
7	0.12336542959794433	0.7248748975842375
8	0.07594369789619423	0.5099801498137448
9	0.05612815476220617	0.4240282770430196
>10	0.135640832115094	1.593538391022318
>50	0.001156695017728838	0.06405610098882135
>100	5.15252637641402E-4	0.07661028192261438
>500	1.9490958447672232E-5	0.015019221484775329
>1k	1.949095382578554E-5	0.025571936398771256
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.013538467960123E-5	0.0
5	0.0	0.0	0.0	8.027076935920246E-5	0.0
6	0.0	0.0	0.0	8.027076935920246E-5	0.0
7	0.0	0.0	0.0	8.027076935920246E-5	0.0
8	0.0	0.0	0.0	8.027076935920246E-5	0.0
9	0.0	0.0	0.0	2.1071076956790646E-4	0.0
10-11	1.0033846169900308E-5	0.0	0.0	2.1071076956790646E-4	0.0
12-13	2.0067692339800615E-5	0.0	0.0	2.2074461573780676E-4	0.0
14-15	2.0067692339800615E-5	0.0	0.0	2.4582923116255755E-4	0.0
16-17	2.0067692339800615E-5	0.0	0.0	2.508461542475077E-4	0.0
18-19	2.508461542475077E-5	0.0	0.0	3.0101538509700926E-4	0.0
20-21	3.0101538509700924E-5	0.0	0.0	3.5118461594651075E-4	0.0
22-23	4.013538467960123E-5	0.0	0.0	4.013538467960123E-4	0.0
24-25	4.013538467960123E-5	0.0	0.0	4.0637076988096245E-4	0.0
26-27	4.013538467960123E-5	0.0	0.0	4.465061545605637E-4	0.0
28-29	4.013538467960123E-5	0.0	0.0	8.428430782716259E-4	0.0
30-31	4.013538467960123E-5	0.0	0.0	0.002056938464829563	0.0
32-33	4.515230776455138E-5	0.0	0.0	0.0046858061613434445	0.0
34-35	5.0169230849501536E-5	0.0	0.0	0.008413380013461407	0.0
36-37	5.0169230849501536E-5	0.0	0.0	0.013921961560736676	0.0
38-39	5.0169230849501536E-5	0.0	0.0	0.02254103542068104	0.0
40-41	5.0169230849501536E-5	0.0	0.0	0.032710338513875004	0.0
42-43	5.0169230849501536E-5	0.0	0.0	0.041515038527962525	0.0
44-45	5.0169230849501536E-5	0.0	0.0	0.05148868162084343	0.0
46-47	5.0169230849501536E-5	0.0	0.0	0.06301757087005888	0.0
48-49	5.0169230849501536E-5	0.0	0.0	0.07384409088738131	0.0
50-51	5.0169230849501536E-5	0.0	0.0	0.0846856616739586	0.0
52-53	5.0169230849501536E-5	0.0	0.0	0.0944586278434415	0.0
54-55	5.0169230849501536E-5	0.0	0.0	0.10502426786034652	0.0
56-57	5.0169230849501536E-5	0.0	0.0	0.11632237864765427	0.0
58-59	5.0169230849501536E-5	0.0	0.0	0.12651174943318802	0.0
60-61	5.0169230849501536E-5	0.0	0.0	0.13607400483310303	0.0
62-63	5.0169230849501536E-5	0.0	0.0	0.14476833253932164	0.0
64-65	5.0169230849501536E-5	0.0	0.0	0.15464163717050355	0.0
66-67	5.0169230849501536E-5	0.0	0.0	0.16432931564754227	0.0
68-69	5.0169230849501536E-5	0.0	0.0	0.174222687971064	0.0
70-71	5.0169230849501536E-5	0.0	0.0	0.1844572110643623	0.0
72-73	5.0169230849501536E-5	0.0	0.0	0.19232374646156414	0.0
74-75	5.0169230849501536E-5	0.0	0.0	0.19928723570347495	0.0
76-77	5.518615393445169E-5	0.0	0.0	0.20594970956028877	0.0
78-79	6.020307701940185E-5	0.0	0.0	0.21207035572392796	0.0
80-81	6.020307701940185E-5	0.0	0.0	0.21594342034550948	0.0
82-83	6.020307701940185E-5	0.0	0.0	0.2188482188116956	0.0
84-85	6.020307701940185E-5	0.0	0.0	0.22176305112405165	0.0
86-87	6.020307701940185E-5	0.0	0.0	0.22233999727882092	0.0
88-89	6.020307701940185E-5	0.0	1.0033846169900308E-5	0.22255070804838883	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	12945	0.0	39.431393	1
GGTATCA	11360	0.0	35.974323	1
TATACAC	17940	0.0	31.498396	3
GTATCAA	18425	0.0	28.907202	1
TTATACA	18975	0.0	28.340614	2
ATCAACG	22835	0.0	22.873398	3
ACACATC	22775	0.0	22.864109	6
TACACAT	24275	0.0	22.691061	5
CAACGCA	23085	0.0	22.522768	5
ACATCTC	23240	0.0	22.406631	8
TCAACGC	23320	0.0	22.329763	4
AACGCAG	24025	0.0	21.69099	6
TATCAAC	24635	0.0	21.475594	2
CACATCT	24620	0.0	21.022018	7
CATCTCC	25950	0.0	19.975113	9
ATACACA	30185	0.0	19.796343	4
ACGCAGA	27685	0.0	18.766195	7
TACATGG	25205	0.0	18.648989	2
CGCAGAG	27890	0.0	18.614058	8
GTACATG	26190	0.0	18.431448	1
>>END_MODULE