FastQCFastQC Report
Fri 27 May 2016
SRR2139863_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139863_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14992646
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA204350.032.9252131
CTTATAC161950.032.304871
TATACAC208150.027.460843
GTATCAA310100.026.5741351
TTATACA219150.024.5974662
ATCAACG343850.023.1458473
ACACATC241100.021.8474866
CAACGCA365200.021.7818475
TCAACGC367400.021.7594574
ACGCAGA364950.021.6118647
AACGCAG366500.021.4554796
TATCAAC396000.020.5089072
ACATCTC262050.019.9796738
TACACAT281700.019.8541765
GTACATG407050.019.698681
ATACACA318450.019.3828164
TACATGG408800.019.1190762
ACATGGG404750.018.7315733
CGCAGAG432500.018.2455948
CACATCT294500.017.7646987
Produced by FastQC (version 0.11.5)