##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139863_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14992646
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79063882386071	31.0	31.0	34.0	30.0	34.0
2	31.959341799973135	31.0	31.0	34.0	30.0	34.0
3	32.69895514107383	34.0	31.0	34.0	30.0	37.0
4	35.56394875194145	37.0	35.0	37.0	33.0	37.0
5	35.4096723820465	37.0	35.0	37.0	33.0	37.0
6	35.39465195136336	37.0	35.0	37.0	33.0	37.0
7	35.35218072913881	37.0	35.0	37.0	32.0	37.0
8	35.5913974091031	37.0	35.0	37.0	32.0	39.0
9	36.77217337086462	39.0	37.0	39.0	32.0	39.0
10-11	36.639299060352656	39.0	35.0	39.0	32.0	39.0
12-13	36.74077084191809	39.0	35.5	39.0	32.0	39.5
14-15	37.67440864007594	39.0	37.0	41.0	32.0	41.0
16-17	37.67025470353933	39.0	37.0	41.0	32.0	41.0
18-19	37.6441526732506	39.0	37.0	41.0	32.0	41.0
20-21	37.605993348242066	39.0	36.5	41.0	32.0	41.0
22-23	37.5123929844477	39.0	36.0	41.0	32.0	41.0
24-25	37.38378060624031	39.0	36.0	41.0	32.0	41.0
26-27	37.196162980325006	39.0	36.0	40.0	31.0	41.0
28-29	37.05673285268297	39.0	36.0	40.0	31.0	41.0
30-31	36.94096239455096	39.0	36.0	40.0	31.0	41.0
32-33	37.10155971521258	39.0	36.0	40.0	31.0	41.0
34-35	37.07690823174343	39.0	36.0	40.0	31.0	41.0
36-37	36.937860181068245	39.0	35.5	40.0	30.5	41.0
38-39	36.77792408382187	39.0	35.0	40.0	30.0	41.0
40-41	36.74987582224588	38.5	35.0	40.0	30.0	41.0
42-43	36.632190680503264	38.0	35.0	40.0	30.0	41.0
44-45	36.728263518539094	38.5	35.0	40.0	30.0	41.0
46-47	36.8495608118765	39.0	35.0	40.0	30.0	41.0
48-49	36.784223541308194	39.0	35.0	40.0	30.0	41.0
50-51	36.63193942509663	39.0	35.0	40.0	30.0	41.0
52-53	36.50310601456851	38.0	35.0	40.0	30.0	41.0
54-55	36.35652083804784	38.0	35.0	40.0	30.0	41.0
56-57	36.17500661090722	38.0	34.0	40.0	29.0	41.0
58-59	36.03278087761349	38.0	34.0	40.0	29.0	41.0
60-61	35.81155921439751	38.0	34.0	40.0	28.5	41.0
62-63	35.55361599691976	37.0	34.0	40.0	28.0	41.0
64-65	35.32041523806658	37.0	34.0	39.5	28.0	41.0
66-67	35.041561058308986	36.0	33.0	39.0	28.0	40.5
68-69	34.70738268164699	36.0	33.0	39.0	28.0	40.0
70-71	34.3699721184915	35.5	32.5	38.5	27.5	40.0
72-73	34.06265631159199	35.0	32.0	38.0	27.0	40.0
74-75	33.587771816940865	35.0	31.5	37.0	26.0	39.0
76-77	32.75160326581589	34.0	31.0	36.0	26.0	39.0
78-79	33.03948861116099	34.0	31.0	36.0	26.0	39.0
80-81	32.88264455488438	34.0	31.0	36.0	26.0	37.5
82-83	32.439941042977885	34.0	31.0	35.5	26.0	37.0
84-85	32.22770284097743	34.0	31.0	35.0	26.0	37.0
86-87	32.112474446096414	34.0	31.0	35.0	26.0	36.0
88-89	31.94712152059375	34.0	31.0	35.0	26.0	36.0
90-91	31.81809206756806	34.0	31.0	35.0	25.5	35.5
92-93	31.731659051246947	34.0	31.0	35.0	25.0	35.0
94-95	31.684671991570504	34.0	31.0	35.0	25.0	35.0
96-97	31.64441022958987	34.0	30.0	35.0	25.0	35.0
98-99	31.86896069801596	34.0	31.0	35.0	26.0	35.0
100-101	30.829070560718932	32.5	28.5	34.5	22.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	7.0
19	29.0
20	237.0
21	1225.0
22	5203.0
23	16177.0
24	40391.0
25	81484.0
26	141144.0
27	218272.0
28	306664.0
29	401982.0
30	505543.0
31	619586.0
32	755482.0
33	936377.0
34	1187798.0
35	1531526.0
36	2077213.0
37	2678642.0
38	2343551.0
39	1101827.0
40	42284.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.50310303393311	0.009691457477146202	26.49493066072328	31.992274847866465
2	14.455231880593447	26.679208011662332	36.07048276796787	22.79507733977636
3	15.506852260805518	25.50024702115564	34.34912710187257	24.643773616166275
4	12.806285161405132	25.4449814929266	36.89765635765695	24.85107698801132
5	16.720250714917167	37.06875357425234	32.903918361041804	13.30707734978869
6	27.291613501712774	38.32522958255667	20.489478641728752	13.8936782740018
7	25.104434534104254	32.8417212011809	23.537746439154237	18.516097825560614
8	24.01955598764888	32.056682989780455	22.398261120818834	21.525499901751832
9	23.10093895367102	20.179486662994645	24.282471553053412	32.43710283028093
10-11	24.483800257806394	26.073172807521765	29.093860416633593	20.349166518038245
12-13	24.449663521702572	24.149689787913353	28.91346864322682	22.48717804715725
14-15	21.938693037745562	26.654718134465543	26.357965792073994	25.0486230357149
16-17	21.460876296729158	28.805421374902927	27.688878116046943	22.044824212320975
18-19	21.535744014983553	28.227873514918183	28.88332386189306	21.353058608205203
20-21	22.16707568800952	27.66889719794615	28.537535992193856	21.62649112185047
22-23	22.018515151565698	27.55513595292147	28.315564903946395	22.110783991566432
24-25	21.921302729432483	27.763086867761388	28.56958078614511	21.74602961666102
26-27	21.82552671200496	28.046240425356938	28.397120769810535	21.731112092827566
28-29	21.737772643467913	27.659496104956048	28.451726504530388	22.15100474704565
30-31	21.896638998087575	27.84926515530729	28.483689256410035	21.770406590195098
32-33	21.643735249852625	27.924416060528458	28.593409813960776	21.83843887565815
34-35	21.69333566865501	27.73405843157251	28.54167662471927	22.030929275053207
36-37	21.841643261952314	27.951659764968916	28.598418461759845	21.60827851131892
38-39	21.656948846428193	27.954737066142222	28.674427036328808	21.713887051100773
40-41	21.904494352159283	27.63099970270591	28.47686513823619	21.98764080689861
42-43	22.04347286716469	27.710378896096927	28.350912740350825	21.895235496387556
44-45	21.75882904340948	28.20042971001584	28.133828856588323	21.906912389986356
46-47	22.080866591514415	27.714671747416226	27.74753175393263	22.45692990713673
48-49	21.969407248967798	27.78629008836208	28.0654810977018	22.17882156496832
50-51	21.916744497232614	28.00371923096333	27.71625828194152	22.36327798986254
52-53	21.95505459875633	27.85846988038839	27.9663204832622	22.220155037593077
54-55	21.88819208641044	28.041831596854323	27.890404121573965	22.17957219516127
56-57	22.159549852975598	28.317424253048557	27.317661422921613	22.205364471054235
58-59	22.0232694289006	28.164090748859167	27.82399927741981	21.988640544820427
60-61	22.180358951844532	28.383165150000885	27.423579372896807	22.012896525257776
62-63	22.063512270228465	28.472086476392278	27.406590308008372	22.057810945370882
64-65	21.98297419845802	28.217245469275387	27.429537756090173	22.37024257617642
66-67	22.02615222211036	28.16711974804498	27.503315879723022	22.303412150121645
68-69	22.051475295502403	28.091161650427647	27.586958628247814	22.27040442582214
70-71	22.1356784501391	27.746318767110665	27.62101330747389	22.496989475276344
72-73	22.07646130244347	27.986939991551235	27.606678969250954	22.32991973675434
74-75	22.033289453507255	28.01191537952083	27.67591412923311	22.278881037738806
76-77	22.17821385404537	27.8662454122017	27.49172722314756	22.46381351060537
78-79	21.980683210882827	27.83479022657236	27.68055689793154	22.503969664613273
80-81	22.012016827525528	27.946332138619965	27.64463942546439	22.397011608390113
82-83	22.13829898883246	27.742373309267354	27.687079939065246	22.43224776283494
84-85	22.21815286799216	27.75330614354144	27.66321693559209	22.365324052874318
86-87	21.98417955537301	27.99940219284121	27.647003348649374	22.369414903136402
88-89	22.11501174144421	27.693678371278256	27.68511374059748	22.50619614668005
90-91	22.118955193346135	27.747868447609243	27.74542400679415	22.387752352250477
92-93	21.9900850132995	27.735914426733327	27.836008282727803	22.437992277239374
94-95	21.92217383753473	27.62878001820348	27.919319856539172	22.529726287722625
96-97	21.618059111546735	27.853431710019883	28.1408358897764	22.38767328865698
98-99	21.915488103736628	28.948918923037674	28.23370296460389	20.901890008621805
100-101	24.845829442312652	36.565183442518375	29.421274538765207	9.167712576403769
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2165.0
1	2182.5
2	3918.0
3	5896.5
4	7882.5
5	12040.5
6	15057.0
7	17109.5
8	18280.5
9	17584.0
10	16169.0
11	14904.5
12	16780.5
13	22799.0
14	36775.0
15	57721.0
16	74809.5
17	82114.5
18	81911.5
19	78781.0
20	74955.0
21	72450.0
22	73864.0
23	81338.0
24	96091.5
25	117080.0
26	143895.5
27	174529.0
28	208473.5
29	249188.0
30	289334.0
31	325704.0
32	363479.5
33	400292.5
34	436379.5
35	474945.0
36	515130.5
37	554235.5
38	584209.0
39	602773.0
40	617721.0
41	628953.0
42	636864.0
43	649418.0
44	665194.5
45	680726.0
46	692395.5
47	700946.0
48	712787.5
49	703672.5
50	676695.5
51	655795.0
52	629055.0
53	587483.5
54	539557.5
55	488984.0
56	440889.5
57	395084.0
58	345123.5
59	294621.0
60	244784.5
61	197217.5
62	156530.5
63	123352.0
64	94785.0
65	71382.0
66	52794.0
67	39741.5
68	30084.5
69	22253.5
70	16630.5
71	12315.0
72	8946.0
73	6326.5
74	4443.0
75	3106.5
76	2162.5
77	1487.5
78	1047.5
79	768.0
80	570.5
81	427.0
82	336.0
83	243.0
84	164.5
85	106.0
86	62.0
87	39.0
88	23.5
89	18.0
90	15.0
91	12.5
92	9.0
93	4.0
94	1.5
95	2.0
96	1.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.068661395726945E-4
2	6.803335448592597E-4
3	2.0009810142919402E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	6.336439878591144E-5
16-17	0.0014206965201472776
18-19	0.0013740069631471322
20-21	1.2674025628414044E-4
22-23	0.002411915623785598
24-25	2.1357224948976257E-4
26-27	0.0010484096477059778
28-29	5.247173277570253E-4
30-31	2.779777859578416E-4
32-33	0.0026752043775485016
34-35	0.01678582519809047
36-37	0.028856378933761393
38-39	0.005647098547918897
40-41	0.004915863690152312
42-43	0.006520290592769947
44-45	0.004629391913181786
46-47	0.0026534238553995912
48-49	0.001193939242116668
50-51	2.005252498980942E-4
52-53	3.348451655261928E-4
54-55	0.005554003240470984
56-57	0.011094073169596073
58-59	0.00954319549885578
60-61	0.006539790459987555
62-63	0.001999496094405862
64-65	0.0012417467817545178
66-67	0.00108726548726456
68-69	0.0
70-71	6.11650696248541E-5
72-73	4.1798967067483187E-4
74-75	3.9365447091934156E-4
76-77	1.3358029412446155E-4
78-79	5.726742653657073E-4
80-81	0.002539194503305049
82-83	0.0048534077600803985
84-85	0.011948518668411522
86-87	0.015315568873066659
88-89	0.05767986997636491
90-91	0.0
92-93	4.9858548527906964E-5
94-95	8.215224638136589E-4
96-97	0.006315182240959609
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	772.0
20-21	2768.0
22-23	4425.0
24-25	7048.0
26-27	12282.0
28-29	25355.0
30-31	47576.0
32-33	67053.0
34-35	97321.0
36-37	141506.0
38-39	155458.0
40-41	157345.0
42-43	173636.0
44-45	199335.0
46-47	199934.0
48-49	191686.0
50-51	175752.0
52-53	178305.0
54-55	195275.0
56-57	206897.0
58-59	212593.0
60-61	210752.0
62-63	199268.0
64-65	207540.0
66-67	220062.0
68-69	207112.0
70-71	203334.0
72-73	193407.0
74-75	195049.0
76-77	199330.0
78-79	213149.0
80-81	218676.0
82-83	210653.0
84-85	212563.0
86-87	226112.0
88-89	249670.0
90-91	274981.0
92-93	306118.0
94-95	387928.0
96-97	545278.0
98-99	1693650.0
100-101	6165692.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.28262008441835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.31976093889826	71.78861574343118
2	7.205061817792909	11.712926048408194
3	2.1912740508682127	5.34337488532696
4	0.9756878356075362	3.172258546507032
5	0.4843461433006689	1.9684461777630762
6	0.29229832403382505	1.425526417425217
7	0.1591814816429215	0.9057081527799481
8	0.10171621119570208	0.6614208120837369
9	0.07571181875687365	0.553864949992377
>10	0.1915129963874975	2.188674294199356
>50	0.0026504766124437495	0.1487972864583807
>100	7.6770452953059E-4	0.11492168048574146
>500	3.020037365210131E-5	0.015465005138777101
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	1.3339873428612934E-5
3	0.0	0.0	0.0	0.0	1.3339873428612934E-5
4	0.0	0.0	0.0	2.6679746857225868E-5	1.3339873428612934E-5
5	0.0	0.0	0.0	2.6679746857225868E-5	1.3339873428612934E-5
6	0.0	0.0	0.0	4.0019620285838803E-5	3.3349683571532334E-5
7	0.0	0.0	0.0	4.0019620285838803E-5	3.3349683571532334E-5
8	0.0	0.0	0.0	4.0019620285838803E-5	3.3349683571532334E-5
9	0.0	0.0	0.0	1.0004905071459701E-4	3.3349683571532334E-5
10-11	0.0	0.0	0.0	1.0004905071459701E-4	3.3349683571532334E-5
12-13	0.0	0.0	0.0	1.2005886085751641E-4	3.668465192868557E-5
14-15	0.0	0.0	0.0	1.56743512786202E-4	7.670427221452438E-5
16-17	0.0	0.0	0.0	1.9009319635773434E-4	8.337420892883084E-5
18-19	0.0	0.0	0.0	2.5012262678649253E-4	8.670917728598407E-5
20-21	6.669936714306467E-6	0.0	0.0	3.3683180407247657E-4	9.004414564313731E-5
22-23	6.669936714306467E-6	3.3349683571532335E-6	0.0	4.0353117121554125E-4	1.3006376592897612E-4
24-25	6.669936714306467E-6	6.669936714306467E-6	0.0	5.269250004302109E-4	1.6674841785766168E-4
26-27	6.669936714306467E-6	6.669936714306467E-6	0.0	7.803825955738567E-4	1.7341835457196814E-4
28-29	1.3339873428612934E-5	6.669936714306467E-6	0.0	0.0018142227862913592	1.7341835457196814E-4
30-31	1.3339873428612934E-5	6.669936714306467E-6	0.0	0.0036484553827256377	1.7341835457196814E-4
32-33	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.007116822474165001	1.9342816471488755E-4
34-35	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.012256008712538133	2.0676803814350048E-4
36-37	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.019436195585489046	2.167729432149602E-4
38-39	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.029447770593663054	2.301128166435731E-4
40-41	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.03982285715276676	2.4011772171503282E-4
42-43	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.04898068026150955	2.4345269007218607E-4
44-45	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.05995606112490083	2.467876584293393E-4
46-47	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.07115488486822139	2.5012262678649253E-4
48-49	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.08204022158596955	2.5345759514364575E-4
50-51	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.09269878045543128	2.5345759514364575E-4
52-53	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.10285709407132004	2.901422470723313E-4
54-55	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.11333556464949548	3.268268990010169E-4
56-57	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.1234538586450984	3.268268990010169E-4
58-59	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.1323382143485546	3.268268990010169E-4
60-61	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.1409324278049385	3.268268990010169E-4
62-63	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.14905641072296377	3.4016677242962984E-4
64-65	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.15725376294484641	3.4016677242962984E-4
66-67	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.16556783905922945	3.4016677242962984E-4
68-69	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.1745422389083288	3.4350174078678306E-4
70-71	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.1830430732507124	3.468367091439363E-4
72-73	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.19054008211759285	3.801863927154686E-4
74-75	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.19730339794589963	3.801863927154686E-4
76-77	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.2035564636155619	3.801863927154686E-4
78-79	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.20911585586693637	3.801863927154686E-4
80-81	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.21295106947766257	3.901912977869283E-4
82-83	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.21588250666360026	3.9352626614408154E-4
84-85	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.21826367407060768	3.9352626614408154E-4
86-87	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.21866053530510893	3.968612345012348E-4
88-89	1.3339873428612934E-5	1.3339873428612934E-5	0.0	0.21877392422925213	4.0019620285838803E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20435	0.0	32.925213	1
CTTATAC	16195	0.0	32.30487	1
TATACAC	20815	0.0	27.46084	3
GTATCAA	31010	0.0	26.574135	1
TTATACA	21915	0.0	24.597466	2
ATCAACG	34385	0.0	23.145847	3
ACACATC	24110	0.0	21.847486	6
CAACGCA	36520	0.0	21.781847	5
TCAACGC	36740	0.0	21.759457	4
ACGCAGA	36495	0.0	21.611864	7
AACGCAG	36650	0.0	21.455479	6
TATCAAC	39600	0.0	20.508907	2
ACATCTC	26205	0.0	19.979673	8
TACACAT	28170	0.0	19.854176	5
GTACATG	40705	0.0	19.69868	1
ATACACA	31845	0.0	19.382816	4
TACATGG	40880	0.0	19.119076	2
ACATGGG	40475	0.0	18.731573	3
CGCAGAG	43250	0.0	18.245594	8
CACATCT	29450	0.0	17.764698	7
>>END_MODULE