FastQCFastQC Report
Fri 27 May 2016
SRR2139862_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139862_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9183047
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC122550.044.2145771
TATACAC145400.038.9323043
TTATACA153850.036.1230662
GGTATCA163400.035.665021
GTATCAA252150.029.9966221
TACACAT217450.026.1604775
ACACATC217250.024.923016
ATACACA241550.024.6029174
ATCAACG301400.024.4753173
CAACGCA301850.024.4126425
TCAACGC308400.023.9843184
AACGCAG311350.023.8209926
ACATCTC231150.023.6648868
TATCAAC317550.023.5185282
CACATCT241150.022.5682417
TACATGG343050.021.4692422
ACATGGG339950.021.1272543
GTACATG358250.020.935351
ACGCAGA354150.020.829997
CGCAGAG353800.020.828148
Produced by FastQC (version 0.11.5)