##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139862_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9183047
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19854129027108	33.0	31.0	34.0	31.0	34.0
2	32.44541882449257	34.0	31.0	34.0	31.0	34.0
3	32.5442579135226	34.0	31.0	34.0	31.0	34.0
4	36.131338759346434	37.0	35.0	37.0	35.0	37.0
5	35.829065015130595	37.0	35.0	37.0	35.0	37.0
6	35.83000838392747	37.0	35.0	37.0	35.0	37.0
7	35.817760161741525	37.0	35.0	37.0	35.0	37.0
8	35.80782587740213	37.0	35.0	37.0	35.0	37.0
9	37.61279235530429	39.0	37.0	39.0	35.0	39.0
10-11	37.45721115224609	39.0	37.0	39.0	35.0	39.0
12-13	37.44679728852526	39.0	37.0	39.0	35.0	39.0
14-15	38.71003709335257	40.0	38.0	41.0	35.0	41.0
16-17	38.63645465388558	40.0	38.0	41.0	35.0	41.0
18-19	38.678520266748066	40.0	38.0	41.0	35.0	41.0
20-21	38.71065203792355	40.0	38.0	41.0	35.0	41.0
22-23	38.64371305578116	40.0	38.0	41.0	35.0	41.0
24-25	38.59827563452855	40.0	38.0	41.0	35.0	41.0
26-27	38.43839503891475	40.0	38.0	41.0	34.0	41.0
28-29	38.32827984539601	40.0	38.0	41.0	34.0	41.0
30-31	38.23681477867093	40.0	38.0	41.0	34.0	41.0
32-33	38.11361039222901	40.0	37.5	41.0	34.0	41.0
34-35	38.036582688569894	40.0	37.0	41.0	34.0	41.0
36-37	37.99208296960238	40.0	37.0	41.0	33.5	41.0
38-39	37.8947866486924	40.0	37.0	41.0	33.0	41.0
40-41	37.833520931685456	40.0	37.0	41.0	33.0	41.0
42-43	37.81560044384513	40.0	37.0	41.0	33.0	41.0
44-45	37.74057335484492	39.0	37.0	41.0	33.0	41.0
46-47	37.631940241127964	39.0	37.0	40.0	33.0	41.0
48-49	37.636051982438175	39.0	37.0	40.0	33.0	41.0
50-51	37.48427112932608	39.0	36.0	40.0	32.5	41.0
52-53	37.2873900490931	39.0	36.0	40.0	32.0	41.0
54-55	37.05742254159239	39.0	35.5	40.0	31.5	41.0
56-57	36.886422769896484	39.0	35.0	40.0	31.0	41.0
58-59	36.70565606296619	38.0	35.0	40.0	31.0	41.0
60-61	36.442415622258785	38.0	35.0	40.0	31.0	41.0
62-63	36.289302857304605	38.0	35.0	40.0	31.0	41.0
64-65	36.020537198731276	37.0	34.0	40.0	30.5	41.0
66-67	35.809579351219085	37.0	34.0	39.0	30.0	41.0
68-69	35.570497626080574	36.0	34.0	39.0	30.0	40.5
70-71	35.144336112761586	36.0	34.0	39.0	30.0	40.0
72-73	34.88594609732647	35.0	34.0	38.5	29.0	40.0
74-75	34.52636131249387	35.0	33.5	37.5	29.0	39.5
76-77	32.63484422911966	33.5	31.0	35.5	27.0	38.5
78-79	33.82973097716877	35.0	33.0	36.0	29.0	39.0
80-81	33.868347389341906	35.0	33.0	36.0	29.0	38.5
82-83	33.61303196649042	35.0	33.0	36.0	29.0	37.0
84-85	33.37942363428484	35.0	33.0	35.0	29.0	37.0
86-87	33.206384264616574	35.0	33.0	35.0	29.0	36.5
88-89	33.070708331117004	34.5	33.0	35.0	29.0	36.0
90-91	33.03926909238479	34.0	33.0	35.0	29.0	36.0
92-93	32.92406995453088	34.0	32.5	35.0	29.0	35.5
94-95	32.8122883816647	34.0	32.0	35.0	29.0	35.0
96-97	32.877968411629254	34.0	32.0	35.0	29.0	35.0
98-99	32.99380188290077	34.0	32.0	35.0	29.0	35.0
100-101	31.77001545278607	33.0	31.0	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	2.0
18	14.0
19	27.0
20	88.0
21	306.0
22	1085.0
23	3072.0
24	7615.0
25	16477.0
26	29382.0
27	48434.0
28	73622.0
29	105603.0
30	146606.0
31	201024.0
32	278129.0
33	400063.0
34	607756.0
35	954165.0
36	1571082.0
37	2232767.0
38	1898528.0
39	600339.0
40	6859.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.94693765587827	20.301028623723695	13.07405918754418	24.677974532853856
2	15.419293266148143	21.221606154693205	39.97405634077667	23.38504423838198
3	18.07920500322824	26.812497964996524	30.765125370915115	24.343171660860126
4	12.41325455483349	18.25131680149301	37.7458048510478	31.589623792625694
5	12.883109495138163	38.17354958544805	35.61999628227973	13.323344637134058
6	29.863522799033298	37.552274589161996	18.63726654004863	13.946936071756078
7	26.19926697532965	32.59531395189418	23.01904803492784	18.186371037848332
8	25.487760217278645	35.22762107174231	21.453979272892756	17.83063943808629
9	26.201515945963454	15.345356578346289	20.480722897470283	37.97240457821997
10-11	24.591669922678964	26.02543696991793	28.657291311391077	20.72560179601203
12-13	25.511167316261947	23.768348459883946	28.301494970257895	22.418989253596212
14-15	22.72626395138781	24.91969713320644	25.878491093424653	26.475547821981095
16-17	22.364847963862104	27.893519438591568	26.662996497785542	23.07863609976079
18-19	22.175079796498917	27.12401450194037	28.279752896832612	22.421152804728102
20-21	22.964098736316117	26.336986216634696	27.851331192598806	22.84758385445038
22-23	22.771366674218395	26.83031046321146	27.6790467829633	22.719276079606846
24-25	22.61768572359752	26.96341245986753	28.030390393799465	22.38851142273548
26-27	22.418903619950882	26.95282062494489	28.093053434557053	22.535222320547177
28-29	21.97712592556388	26.93729570144076	28.36654487009089	22.719033502904466
30-31	22.50348380849157	26.68787022299436	27.695435532922097	23.11321043559197
32-33	22.651196526859348	26.82378362735974	28.160699282873214	22.364320562907693
34-35	22.704885934100727	27.404304641955502	28.00246494752689	21.888344476416883
36-37	22.521592942304068	26.98814691622427	27.743728267322066	22.7465318741496
38-39	22.394416044661213	26.81806503442965	28.054796796066878	22.73272212484226
40-41	22.458038056326284	26.671789627102978	28.28913428965341	22.58103802691733
42-43	22.77135413454821	26.616494363475468	27.97420285914645	22.637948642829873
44-45	22.285716287927233	27.321785737019173	27.678444934597294	22.714053040456296
46-47	22.99473651162109	26.712592263575385	26.98032474024709	23.312346484556436
48-49	22.797482858219627	26.82807078204422	27.126660981071403	23.247785378664744
50-51	22.644477850262202	26.944860249518943	27.23853108168767	23.17213081853119
52-53	22.634078300147817	27.11904731033813	27.47071202892974	22.77616236058432
54-55	22.653083397182105	27.241180014661886	26.929949879945013	23.175786708210993
56-57	23.056248685993495	27.58121391437614	26.388243075473465	22.9742943241569
58-59	22.491767430823547	27.834844879729438	27.165737781407273	22.507649908039742
60-61	23.05718113925526	28.283226147173057	26.231131334478793	22.428461379092894
62-63	22.718116584035602	28.26884007150381	26.283096292049112	22.72994705241147
64-65	22.741959923753512	27.889945941975164	26.40220675431954	22.96588737995178
66-67	22.816266450181555	27.717557114219034	26.412042127006924	23.054134308592488
68-69	22.93046348733074	27.32813669730448	26.619161787592244	23.12223802777254
70-71	23.017341579907082	26.893662191704376	26.68779630983223	23.401199918556312
72-73	23.035396246016376	27.096719056494738	26.720499366986044	23.147385330502836
74-75	22.98297500616717	27.182738060609175	26.703555431084723	23.13073150213893
76-77	23.028051432271628	27.08163395757335	26.65111737050766	23.23919723964736
78-79	22.89346790796496	27.152340653310887	26.565876188575565	23.388315250148587
80-81	22.958114951665628	27.048796277193514	26.722507289569275	23.270581481571583
82-83	23.230304551987484	26.86619925811817	26.85820785667317	23.04528833322118
84-85	23.250166504012654	27.001615364896253	26.580999040429916	23.167219090661177
86-87	23.04009056352133	27.072298580103343	26.717611420400782	23.169999435974542
88-89	23.202700593868357	26.865744475468812	26.542606483804214	23.388948446858617
90-91	23.1849203797989	26.838808410326603	26.714582231577783	23.261688978296714
92-93	23.28047228649118	26.80753135267606	26.748364864659642	23.163631496173117
94-95	22.934510412018184	26.867303272524047	26.884174420575864	23.314011894881908
96-97	23.046106193738506	26.89360154781344	26.843322458537134	23.21696979991092
98-99	23.2164396675769	27.479447686979224	26.860992673649285	22.44311997179459
100-101	27.758776910818888	34.402846325141184	27.388372862402466	10.450003901637462
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1392.0
1	1547.5
2	3153.0
3	4798.0
4	6934.0
5	12164.0
6	16116.5
7	18001.5
8	18451.5
9	16834.0
10	14354.5
11	12153.5
12	13319.5
13	18888.0
14	30757.0
15	46086.0
16	57793.5
17	63559.0
18	63705.5
19	59425.0
20	52853.5
21	46206.0
22	42186.5
23	43224.0
24	48842.5
25	58495.0
26	72254.0
27	87161.5
28	102403.5
29	121395.5
30	140155.5
31	157669.0
32	177946.5
33	196629.5
34	214220.5
35	234597.0
36	257172.0
37	280323.5
38	295354.5
39	305233.0
40	317386.5
41	326603.0
42	335817.0
43	351564.5
44	368585.0
45	388379.0
46	410881.0
47	433900.5
48	471433.5
49	490117.5
50	473364.5
51	462142.0
52	450589.5
53	425768.5
54	397587.0
55	368123.0
56	339087.0
57	305618.0
58	271359.5
59	232820.0
60	188861.5
61	152216.0
62	122422.0
63	95292.0
64	71696.0
65	54100.0
66	40784.5
67	32176.5
68	25684.5
69	19631.0
70	14547.0
71	9991.5
72	6958.0
73	4807.0
74	3224.0
75	2232.5
76	1543.5
77	1020.0
78	693.5
79	491.0
80	349.5
81	250.5
82	195.0
83	135.5
84	84.0
85	55.0
86	37.0
87	27.0
88	22.0
89	18.5
90	11.5
91	5.5
92	4.0
93	3.5
94	3.0
95	2.5
96	2.0
97	2.0
98	2.5
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	7.73163852912873E-4
3	1.742341076986756E-4
4	0.0
5	0.0
6	5.227023230960268E-4
7	0.0
8	0.0
9	2.177926346233445E-5
10-11	3.811371105908529E-4
12-13	2.3412708222009536E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	3.26982747082333E-5
28-29	1.0909923038675404E-5
30-31	1.6402108851939313E-5
32-33	9.331433014445333E-5
34-35	2.207151668013489E-5
36-37	5.614408640452603E-4
38-39	7.867315697733685E-5
40-41	2.2753538843675396E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	5.032803498654008E-4
56-57	6.394188143359232E-5
58-59	0.0
60-61	8.414308352466498E-4
62-63	1.3497395070238418E-5
64-65	1.3756445840631988E-5
66-67	7.018406754290072E-6
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	1.8301677531762562E-4
88-89	5.083181176776775E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	299.0
20-21	1182.0
22-23	2100.0
24-25	3355.0
26-27	6981.0
28-29	15858.0
30-31	34101.0
32-33	44344.0
34-35	59435.0
36-37	90302.0
38-39	109496.0
40-41	104557.0
42-43	116397.0
44-45	135533.0
46-47	132513.0
48-49	125571.0
50-51	114677.0
52-53	108370.0
54-55	124494.0
56-57	134158.0
58-59	137608.0
60-61	139776.0
62-63	138441.0
64-65	141633.0
66-67	141493.0
68-69	122592.0
70-71	114091.0
72-73	105532.0
74-75	105426.0
76-77	103836.0
78-79	110789.0
80-81	112277.0
82-83	105351.0
84-85	103257.0
86-87	107556.0
88-89	113372.0
90-91	122756.0
92-93	124835.0
94-95	135472.0
96-97	195077.0
98-99	690335.0
100-101	4547819.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.15022778699297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.61804784970117	71.91374769041892
2	7.01820763643459	11.39058296706161
3	2.1323840166623707	5.191303460444831
4	0.8873050110649893	2.8802001505785673
5	0.5018691491395039	2.0363397885967545
6	0.2670569263387433	1.3003038242689933
7	0.16245616532643975	0.9228348375149381
8	0.11139745298225444	0.7231942947520629
9	0.0677233991599703	0.4946192341506934
>10	0.22899713049189552	2.7666858567031176
>50	0.003318154854242627	0.1864909839944373
>100	0.0011861054825802512	0.1687396290652999
>500	5.1002361280970225E-5	0.02495728244979025
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0889631731167225E-5	0.0	0.0	0.0	0.0
2	6.533779038700335E-5	0.0	0.0	0.0	0.0
3	6.533779038700335E-5	0.0	0.0	0.0	0.0
4	6.533779038700335E-5	0.0	0.0	2.177926346233445E-5	0.0
5	1.9601337116101006E-4	0.0	0.0	2.177926346233445E-5	0.0
6	2.8313042501034786E-4	0.0	0.0	3.2668895193501676E-5	0.0
7	5.33591954827194E-4	0.0	0.0	3.2668895193501676E-5	0.0
8	5.553712182895285E-4	0.0	0.0	3.2668895193501676E-5	0.0
9	5.553712182895285E-4	0.0	0.0	5.444815865583613E-5	0.0
10-11	6.04374561079781E-4	0.0	0.0	7.622742211817058E-5	0.0
12-13	7.296053259882041E-4	0.0	0.0	9.256186971492142E-5	0.0
14-15	9.637324082082995E-4	0.0	0.0	1.0889631731167225E-4	0.0
16-17	0.0010399598303264701	0.0	0.0	1.1978594904283948E-4	0.0
18-19	0.0013612039663959033	0.0	0.0	1.6334447596750838E-4	0.0
20-21	0.0015681069692880805	0.0	0.0	2.940200567415151E-4	0.0
22-23	0.0017205618135244217	0.0	0.0	3.756922947252693E-4	0.0
24-25	0.0022432641366204485	0.0	0.0	4.682541644401907E-4	0.0
26-27	0.0022650434000827833	0.0	0.0	6.914916149291189E-4	0.0
28-29	0.002744187196254141	0.0	0.0	0.0017804547880458414	0.0
30-31	0.003027317621264489	0.0	0.0	0.003996494845338372	0.0
32-33	0.003054541700592407	0.0	0.0	0.0074321736565216324	0.0
34-35	0.0033376721256027546	0.0	0.0	0.012261725329294296	0.0
36-37	0.0037787022107150276	0.0	0.0	0.01935087558628416	0.0
38-39	0.004187063400633798	0.0	0.0	0.030158835079467632	0.0
40-41	0.0042197322958273005	0.0	0.0	0.04257846006886386	0.0
42-43	0.004432080114585062	0.0	0.0	0.05243902160143578	0.0
44-45	0.004764213882385661	0.0	0.0	0.06436316834706389	0.0
46-47	0.005325029916540773	0.0	0.0	0.0766902314667452	0.0
48-49	0.005575491446357619	0.0	0.0	0.08832580297149736	0.0
50-51	0.005575491446357619	0.0	0.0	0.0999123711334593	0.0
52-53	0.005613605157416705	0.0	0.0	0.1108128924963577	0.0
54-55	0.005755170369921879	0.0	0.0	0.12168074496406259	0.0
56-57	0.006217979718496486	0.0	0.0	0.13251592853657396	0.0
58-59	0.006228869350227653	0.0	0.0	0.14288830276051076	0.0
60-61	0.006261538245421154	0.0	0.0	0.15291765358491577	0.0
62-63	0.006354100115136077	0.0	0.0	0.16297422848864868	0.0
64-65	0.006424882721388663	0.0	0.0	0.17196906429859282	0.0
66-67	0.006838688727173018	0.0	0.0	0.1812470305335473	0.0
68-69	0.006969364307947024	0.0	0.0	0.19030720413387842	0.0
70-71	0.007132708783914533	0.0	0.0	0.19866499648754926	0.0
72-73	0.0072960532598820415	0.0	0.0	0.20501909660268536	0.0
74-75	0.007415839208924881	0.0	0.0	0.20990309643411387	0.0
76-77	0.00745939773584955	0.0	0.0	0.21416638725686582	0.0
78-79	0.007481176999311884	0.0	0.0	0.2179287550199841	0.0
80-81	0.007992989690676744	0.0	0.0	0.21979632686187928	0.0
82-83	0.008058327481063747	0.0	0.0	0.22049870810853958	0.0
84-85	0.008265230483955924	0.0	0.0	0.22111397230135055	0.0
86-87	0.008510247197907186	0.0	0.0	0.22132087530424271	0.0
88-89	0.008570140172428607	0.0	0.0	0.22139710272636087	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	12255	0.0	44.214577	1
TATACAC	14540	0.0	38.932304	3
TTATACA	15385	0.0	36.123066	2
GGTATCA	16340	0.0	35.66502	1
GTATCAA	25215	0.0	29.996622	1
TACACAT	21745	0.0	26.160477	5
ACACATC	21725	0.0	24.92301	6
ATACACA	24155	0.0	24.602917	4
ATCAACG	30140	0.0	24.475317	3
CAACGCA	30185	0.0	24.412642	5
TCAACGC	30840	0.0	23.984318	4
AACGCAG	31135	0.0	23.820992	6
ACATCTC	23115	0.0	23.664886	8
TATCAAC	31755	0.0	23.518528	2
CACATCT	24115	0.0	22.568241	7
TACATGG	34305	0.0	21.469242	2
ACATGGG	33995	0.0	21.127254	3
GTACATG	35825	0.0	20.93535	1
ACGCAGA	35415	0.0	20.82999	7
CGCAGAG	35380	0.0	20.82814	8
>>END_MODULE