FastQCFastQC Report
Fri 27 May 2016
SRR2139861_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139861_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15553370
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC246800.043.945231
TATACAC311050.037.0876273
GGTATCA258450.035.5406071
TTATACA317100.035.193572
GTATCAA415250.028.2109051
TACACAT416450.027.5240885
ACACATC413650.026.406936
ACATCTC443400.024.7663788
ATACACA514250.024.0395364
CACATCT455250.024.010957
CATCTCC486500.022.4925429
ATCAACG508900.022.4781553
TATCAAC531100.021.8599992
CAACGCA521800.021.833245
CGTACCG1253.549951E-521.7223265
TCAACGC526150.021.7117084
AACGCAG526150.021.6306116
TACATGG550600.019.9304182
GTACATG579500.019.2577761
ACGCAGA593750.019.0960467
Produced by FastQC (version 0.11.5)