##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139861_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15553370
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.720802501322865	31.0	31.0	34.0	30.0	34.0
2	31.91216784529655	31.0	31.0	34.0	30.0	34.0
3	31.934663805979024	31.0	31.0	34.0	30.0	34.0
4	35.666897784853056	37.0	35.0	37.0	33.0	37.0
5	35.48041254081913	37.0	35.0	37.0	33.0	37.0
6	35.47940227744855	37.0	35.0	37.0	33.0	37.0
7	35.35657108395158	37.0	35.0	37.0	32.0	37.0
8	35.32680525185217	37.0	35.0	37.0	32.0	37.0
9	36.88025636887697	39.0	37.0	39.0	33.0	39.0
10-11	36.71637837973378	39.0	35.0	39.0	32.0	39.0
12-13	36.66612428689088	39.0	35.0	39.0	32.0	39.0
14-15	37.7542259330293	40.0	37.0	41.0	32.0	41.0
16-17	37.7664309728374	40.0	37.0	41.0	32.0	41.0
18-19	37.76729988420516	40.0	37.0	41.0	32.0	41.0
20-21	37.75036431973608	40.0	37.0	41.0	32.0	41.0
22-23	37.66449902245106	39.0	37.0	41.0	32.0	41.0
24-25	37.54812661184937	39.0	36.0	41.0	32.0	41.0
26-27	37.37308290691595	39.0	36.0	41.0	32.0	41.0
28-29	37.22709818228458	39.0	36.0	40.0	31.0	41.0
30-31	37.06871261022975	39.0	36.0	40.0	31.0	41.0
32-33	37.22842489860753	39.0	36.0	40.0	31.0	41.0
34-35	37.1918537383319	39.0	36.0	40.0	31.0	41.0
36-37	37.085899045782355	39.0	36.0	40.0	31.0	41.0
38-39	36.877620622270825	39.0	35.0	40.0	30.0	41.0
40-41	36.89020813285222	39.0	35.0	40.0	30.0	41.0
42-43	36.78176098375338	39.0	35.0	40.0	30.0	41.0
44-45	36.840775161844164	38.5	35.0	40.0	30.5	41.0
46-47	36.982901712598746	39.0	35.0	40.0	31.0	41.0
48-49	36.92533402674093	39.0	35.0	40.0	30.5	41.0
50-51	36.781798236191385	39.0	35.0	40.0	30.0	41.0
52-53	36.65447874428051	39.0	35.0	40.0	30.0	41.0
54-55	36.53628919936344	38.0	35.0	40.0	30.0	41.0
56-57	36.348165679377246	38.0	35.0	40.0	30.0	41.0
58-59	36.19943866899789	38.0	34.0	40.0	29.0	41.0
60-61	36.00474920775274	38.0	34.0	40.0	29.0	41.0
62-63	35.76381023227651	37.5	34.0	40.0	29.0	41.0
64-65	35.55357769432288	37.0	34.0	40.0	29.0	41.0
66-67	35.28815656983461	36.5	34.0	39.0	28.0	41.0
68-69	34.97278136879878	36.0	33.0	39.0	28.0	40.5
70-71	34.63096963712707	36.0	33.0	39.0	28.0	40.0
72-73	34.346844278370895	35.0	33.0	38.0	28.0	40.0
74-75	33.988223644779424	35.0	32.0	37.5	27.5	39.5
76-77	32.92135042985562	34.0	31.0	36.0	26.0	39.0
78-79	33.27605347299007	35.0	32.0	36.0	26.5	39.0
80-81	33.15694256530339	35.0	32.0	36.0	27.0	38.5
82-83	32.73554490885445	34.0	31.5	35.5	26.0	37.0
84-85	32.495637011303806	34.0	31.0	35.0	26.0	37.0
86-87	32.4006163004107	34.0	31.0	35.0	26.0	36.0
88-89	32.236120903034774	34.0	31.0	35.0	26.0	36.0
90-91	32.10300214224124	34.0	31.0	35.0	26.0	36.0
92-93	32.01529173420646	34.0	31.0	35.0	26.0	35.0
94-95	31.97486264314712	34.0	31.0	35.0	26.0	35.0
96-97	31.89378015823185	34.0	31.0	35.0	26.0	35.0
98-99	32.108607390630844	34.0	31.0	35.0	26.5	35.0
100-101	31.21608150395047	33.0	29.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	2.0
18	6.0
19	47.0
20	210.0
21	1090.0
22	4475.0
23	14561.0
24	36572.0
25	75252.0
26	132543.0
27	206397.0
28	293054.0
29	386834.0
30	491471.0
31	604883.0
32	742280.0
33	920072.0
34	1173039.0
35	1518474.0
36	2087305.0
37	2814299.0
38	2654817.0
39	1356483.0
40	39202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.898043960890796	22.422999002788462	14.470786716962305	25.20817031935844
2	14.174113751807749	22.71485112012806	40.447343973517434	22.663691154546754
3	16.772353308148222	27.935606748608617	31.95688985453891	23.335150088704253
4	11.717364146805483	19.80308447622605	38.48101729721598	29.998534079752492
5	11.986013320585828	38.99001952631488	36.53122763748306	12.49273951561623
6	28.665491787310405	39.23073263222054	18.796428040996904	13.307347539472152
7	25.093635655809642	33.133700284890026	23.637481780475873	18.13518227882446
8	24.382985809506234	36.70174373785231	21.747550530849587	17.167719921791868
9	25.405818803256143	15.629770268437001	20.93352115972294	38.03088976858391
10-11	23.7378844584807	26.88919186002776	29.48851277890258	19.884410902588957
12-13	24.692793266025305	24.35689821562787	29.21114202259703	21.739166495749796
14-15	21.88523130356958	25.660728189453476	26.582875608308683	25.871164898668265
16-17	21.545411265649868	28.737668437575113	27.375283371445175	22.341636925329848
18-19	21.410381048968286	28.003903515803557	28.965473476014537	21.620241959213622
20-21	22.324764068628873	27.1720597021528	28.52914893414549	21.97402729507284
22-23	21.959729567664805	27.81665299404656	28.212953180472667	22.010664257815968
24-25	21.946685583962413	27.9650167265927	28.577894651012404	21.510403038432486
26-27	22.057882565361847	28.10750731881857	28.29254471409862	21.54206540172096
28-29	21.479114117741805	28.302292019006202	28.366197954417334	21.852395908834662
30-31	22.525899076497176	27.77709653325316	28.327519508615417	21.36948488163425
32-33	21.2617679747947	28.463308506140162	28.40893006055904	21.865993458506097
34-35	21.82463770115001	28.370608899851085	28.6999215400545	21.104831858944404
36-37	21.572821718360984	28.381002010934992	28.494737753111266	21.551438517592754
38-39	21.477081971024553	27.623240271909232	28.89399613510767	22.00568162195854
40-41	21.631113822622343	27.445183441400033	29.05120312221512	21.872499613762503
42-43	22.09990891530737	27.454778268521284	28.61179449696421	21.83351831920714
44-45	21.589946757512372	28.26557858248212	28.290651635562842	21.85382302444267
46-47	22.21222931669053	27.586529009054207	27.692557992052507	22.508683682202758
48-49	22.141186377043702	27.544752890480318	27.756519658515888	22.55754107396009
50-51	21.808247676642598	27.59431860670558	28.0132996152439	22.584134101407923
52-53	21.77999124227059	27.761790650921558	28.31478146525343	22.14343664155442
54-55	21.857258565662537	27.877600317347312	27.744541445197257	22.520599671792894
56-57	22.426295960063182	28.115591826080617	27.142878529354753	22.31523368450145
58-59	21.814933075684	28.383433800870566	28.01146900581628	21.790164117629153
60-61	22.273748346541957	28.92353540902512	27.04839276968631	21.754323474746617
62-63	21.90685351000163	29.014169872878607	27.01686672096799	22.06210989615177
64-65	21.957563084715545	28.64153969192832	27.17811174887743	22.2227854744787
66-67	22.1054168118388	28.35272619949475	27.195270105408287	22.346586883258162
68-69	22.16112462987995	28.079219743314876	27.34876613868087	22.410889488124305
70-71	22.20571035945673	27.727985766477854	27.4951729481261	22.571130925939315
72-73	22.25574065919969	27.820346036494854	27.537972244085086	22.385941060220375
74-75	22.207235603322815	27.861480600766047	27.44930824331303	22.481975552598108
76-77	22.16457485933293	27.85881069324438	27.421527273742658	22.555087173680036
78-79	22.09623700588363	27.866390516891	27.424936405636817	22.612436071588554
80-81	22.085710446681077	27.734608661383632	27.574176743841623	22.605504148093665
82-83	22.278379845242817	27.694810630244742	27.608721904346297	22.418087620166144
84-85	22.486416610184598	27.734928690906685	27.350696062588053	22.42795863632066
86-87	22.16034278986299	27.71764390759986	27.597130692985257	22.524882609551895
88-89	22.283290385372	27.560286075503242	27.500058685871352	22.65636485325341
90-91	22.285088779425287	27.53096791938589	27.65254475088696	22.53139855030186
92-93	22.286583260826653	27.553281308059656	27.58639993376275	22.57373549735094
94-95	21.90913923036437	27.585233411264774	27.825781605223217	22.67984575314764
96-97	21.83574441321489	27.657528280824938	27.89694775664292	22.60977954931725
98-99	21.927517182444635	28.291094426228465	27.974438381870133	21.80695000945677
100-101	24.707061233881554	36.07417529772061	29.201178415799404	10.017585052598434
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3087.0
1	3279.5
2	6268.5
3	9488.0
4	12902.5
5	20061.0
6	25250.5
7	28313.5
8	29335.0
9	27115.0
10	23955.5
11	21625.5
12	24884.0
13	36070.5
14	59825.0
15	90388.0
16	111828.0
17	118821.5
18	115661.5
19	108361.0
20	99319.0
21	91409.0
22	88398.5
23	93685.5
24	108565.5
25	132550.0
26	163527.5
27	195093.0
28	227751.5
29	266270.0
30	300976.0
31	333948.5
32	372205.5
33	408558.5
34	445525.5
35	485281.0
36	524264.5
37	563574.0
38	591912.5
39	607347.0
40	621358.5
41	630399.0
42	639086.0
43	655916.0
44	673971.0
45	701400.5
46	756713.0
47	813860.5
48	814345.5
49	787938.5
50	764980.0
51	732071.5
52	692038.0
53	638684.5
54	582682.0
55	530598.5
56	481409.5
57	421706.5
58	359888.0
59	303503.5
60	246779.5
61	196242.5
62	153607.5
63	117920.5
64	87751.0
65	64423.0
66	47088.0
67	35919.5
68	27839.0
69	20377.5
70	14629.0
71	9945.5
72	6846.5
73	4562.5
74	3007.5
75	2032.5
76	1393.0
77	942.5
78	632.0
79	463.0
80	337.5
81	232.5
82	169.5
83	113.0
84	75.0
85	53.0
86	35.0
87	27.5
88	21.0
89	12.0
90	5.5
91	3.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0012408886305668805
3	6.429474769776583E-6
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0015527181569010447
18-19	0.001359833913807747
20-21	2.8934425172795582E-5
22-23	0.002401975986992673
24-25	1.383092142434817E-4
26-27	0.0011585621702804474
28-29	4.832061695763732E-4
30-31	1.54974817560739E-4
32-33	8.947902394465165E-4
34-35	0.016060497016630634
36-37	0.03217131456751113
38-39	0.0069830950214923955
40-41	0.004275235335184182
42-43	0.007085154223095408
44-45	0.005168468289190831
46-47	0.0029923648415572848
48-49	0.0013156838065277615
50-51	1.5350747025829313E-4
52-53	1.039315794656588E-4
54-55	0.0045020869095827865
56-57	0.012082459485187436
58-59	0.00983227038782692
60-61	0.00818324363249592
62-63	0.0018965677222527553
64-65	0.0012954171266056976
66-67	0.0015087019499205566
68-69	0.0
70-71	8.893282565733364E-6
72-73	6.441256264518626E-4
74-75	7.353229538413272E-4
76-77	7.54676399476632E-5
78-79	7.70411684410291E-4
80-81	0.002717775953344846
82-83	0.004881548642843836
84-85	0.012606084035133832
86-87	0.008092965236101058
88-89	0.07041669625919138
90-91	0.0
92-93	0.0
94-95	1.1859289998538935E-4
96-97	0.008741472259836729
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	559.0
20-21	2226.0
22-23	4277.0
24-25	7254.0
26-27	12402.0
28-29	26416.0
30-31	59399.0
32-33	79852.0
34-35	111847.0
36-37	169045.0
38-39	202274.0
40-41	197683.0
42-43	215528.0
44-45	247789.0
46-47	248155.0
48-49	233652.0
50-51	213701.0
52-53	205467.0
54-55	227118.0
56-57	246462.0
58-59	255691.0
60-61	266036.0
62-63	254185.0
64-65	258175.0
66-67	275308.0
68-69	231304.0
70-71	225621.0
72-73	212215.0
74-75	210856.0
76-77	212389.0
78-79	227352.0
80-81	232448.0
82-83	220766.0
84-85	219401.0
86-87	229349.0
88-89	247417.0
90-91	269290.0
92-93	282559.0
94-95	351105.0
96-97	424761.0
98-99	1149750.0
100-101	6586286.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.68696441670656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.38086730102862	73.01251726753493
2	6.54625466906794	10.694873444297038
3	1.9147307639270366	4.692256313414435
4	0.8234216977679124	2.6905127570204623
5	0.4402925317692841	1.7983080187789577
6	0.2656607649432191	1.302061287169907
7	0.17187661383419053	0.9828055186836276
8	0.11566446137995053	0.7558622992817238
9	0.07623543102883154	0.5604696847570004
>10	0.26070756378206267	3.150820948660059
>50	0.003136116897809239	0.17846711042367572
>100	0.001107114899390284	0.14925812087633447
>500	2.9980623604004384E-5	0.018563601062078047
>1k	1.4989050118605575E-5	0.01322362803973066
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	6.429474769776582E-5	0.0	0.0	0.0	1.2858949539553164E-5
3	6.429474769776582E-5	0.0	0.0	0.0	1.2858949539553164E-5
4	6.429474769776582E-5	0.0	0.0	0.0	3.214737384888291E-5
5	1.1573054585597848E-4	0.0	0.0	0.0	3.214737384888291E-5
6	2.250316169421804E-4	0.0	0.0	0.0	4.5006323388436075E-5
7	3.986274357261481E-4	0.0	0.0	0.0	4.5006323388436075E-5
8	4.2434533480525445E-4	0.0	0.0	6.429474769776582E-6	6.429474769776582E-5
9	4.2434533480525445E-4	0.0	0.0	6.429474769776582E-6	8.358317200709557E-5
10-11	4.5327797126924903E-4	0.0	0.0	2.6682320294572816E-4	8.358317200709557E-5
12-13	5.079285068123501E-4	0.0	0.0	2.7646741510039304E-4	8.358317200709557E-5
14-15	6.429474769776582E-4	0.0	0.0	2.957558394097228E-4	1.0287159631642531E-4
16-17	6.815243255963177E-4	0.0	0.0	3.150442637190525E-4	1.2216002062575507E-4
18-19	8.969117303838332E-4	0.0	0.0	3.3433268802838227E-4	1.510926570897497E-4
20-21	0.001080151761322466	0.0	0.0	4.30774809575031E-4	1.6073686924441454E-4
22-23	0.0011315875594806785	0.0	0.0	4.918548198879086E-4	2.2181687955729208E-4
24-25	0.0015623623690557095	0.0	0.0	5.690085171252276E-4	3.054000515643877E-4
26-27	0.0015848655307499275	0.0	0.0	6.750948508265412E-4	3.0861478894927594E-4
28-29	0.0018774066327747621	0.0	0.0	0.0015880802681348157	3.1504426371905256E-4
30-31	0.0020188550777098468	0.0	0.0	0.003494419537373572	3.2468847587371744E-4
32-33	0.0020252845524796233	0.0	0.0	0.007229944378613767	4.1148638526570126E-4
34-35	0.0021892361591089263	0.0	0.0	0.012534261063679447	4.4363375911458415E-4
36-37	0.0024142677760511065	0.0	0.0	0.019947445473231847	4.4684849649947246E-4
38-39	0.0026618025546875047	0.0	0.0	0.03180661168608476	4.725663955785788E-4
40-41	0.0027035941406910527	0.0	0.0	0.044887378105195205	4.886400825030203E-4
42-43	0.003002564717485664	0.0	0.0	0.056142173689689115	4.950695572727968E-4
44-45	0.003381903728902482	0.0	0.0	0.06933545591727067	5.079285068123501E-4
46-47	0.0038062490637077364	0.0	0.0	0.08350280357247336	5.432906180461212E-4
48-49	0.0039798448824917045	0.0	0.0	0.09685682266929932	5.979411535892221E-4
50-51	0.0039798448824917045	0.0	0.0	0.11021727124089506	6.011558909741104E-4
52-53	0.0039927038320312575	0.0	0.0	0.12221145642391326	6.815243255963177E-4
54-55	0.004028065943265029	0.0	0.0	0.13480036802313583	7.779664471429665E-4
56-57	0.004320607045289863	0.0	0.0	0.14660166896306073	8.036843462220728E-4
58-59	0.004339895469599193	0.0	0.0	0.15791111508309774	8.101138209918494E-4
60-61	0.004400975479912071	0.0	0.0	0.16850688950368953	8.165432957616259E-4
62-63	0.004452411278070284	0.0	0.0	0.1784307838108397	8.808380434593918E-4
64-65	0.004500632338843608	0.0	0.0	0.1886440044826298	8.936969929989449E-4
66-67	0.004789958703483554	0.0	0.0	0.198825077780571	9.097706799233863E-4
68-69	0.004873541875490649	0.0	0.0	0.20950765011055483	9.129854173082747E-4
70-71	0.004976413471807075	0.0	0.0	0.21990089607589866	9.354885790024927E-4
72-73	0.005031064007350176	0.0	0.0	0.22843923857016196	0.0010351454379340297
74-75	0.005072855593353723	0.0	0.0	0.23655966520438979	0.0010351454379340297
76-77	0.005088929280278164	0.0	0.0	0.24366423482499291	0.0010351454379340297
78-79	0.005095358755047942	0.0	0.0	0.2504151833332583	0.0010351454379340297
80-81	0.005535777776777637	0.0	0.0	0.25430501556897317	0.0010640780743980242
82-83	0.005622575686169621	0.0	0.0	0.25718220552844817	0.0011315875594806785
84-85	0.005773668343259371	3.214737384888291E-6	0.0	0.26014619339731515	0.0011315875594806785
86-87	0.005892613626500238	6.429474769776582E-6	0.0	0.26054160609565646	0.0011380170342504552
88-89	0.005905472576039791	6.429474769776582E-6	0.0	0.26064447769197285	0.0011508759837900082
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	24680	0.0	43.94523	1
TATACAC	31105	0.0	37.087627	3
GGTATCA	25845	0.0	35.540607	1
TTATACA	31710	0.0	35.19357	2
GTATCAA	41525	0.0	28.210905	1
TACACAT	41645	0.0	27.524088	5
ACACATC	41365	0.0	26.40693	6
ACATCTC	44340	0.0	24.766378	8
ATACACA	51425	0.0	24.039536	4
CACATCT	45525	0.0	24.01095	7
CATCTCC	48650	0.0	22.492542	9
ATCAACG	50890	0.0	22.478155	3
TATCAAC	53110	0.0	21.859999	2
CAACGCA	52180	0.0	21.83324	5
CGTACCG	125	3.549951E-5	21.722326	5
TCAACGC	52615	0.0	21.711708	4
AACGCAG	52615	0.0	21.630611	6
TACATGG	55060	0.0	19.930418	2
GTACATG	57950	0.0	19.257776	1
ACGCAGA	59375	0.0	19.096046	7
>>END_MODULE