FastQCFastQC Report
Fri 27 May 2016
SRR2139860_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139860_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7200736
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC103350.045.4490471
TATACAC134500.036.9783483
GGTATCA115500.035.5086631
TTATACA140500.034.263372
GTATCAA185800.030.0392041
TACACAT183700.026.9473045
ACACATC180000.026.3327966
ATACACA211300.025.049494
ACATCTC190200.024.900158
ATCAACG230800.023.5912133
CACATCT199600.023.5713967
TATCAAC239300.023.0792392
CAACGCA236550.022.9683695
TCAACGC237300.022.912194
AACGCAG239100.022.6419666
CATCTCC214750.021.962919
TACATGG262200.020.0385912
ACGCAGA271850.019.8139827
CGCAGAG273200.019.6733048
GTACATG273100.019.5810871
Produced by FastQC (version 0.11.5)