##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139860_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7200736 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.66313249090093 31.0 31.0 34.0 30.0 34.0 2 31.856166786284067 31.0 31.0 34.0 30.0 34.0 3 31.874945283371034 31.0 31.0 34.0 30.0 34.0 4 35.619880523324284 37.0 35.0 37.0 33.0 37.0 5 35.42729173795568 37.0 35.0 37.0 33.0 37.0 6 35.42634183505686 37.0 35.0 37.0 33.0 37.0 7 35.30257670882532 37.0 35.0 37.0 32.0 37.0 8 35.26894139710163 37.0 35.0 37.0 32.0 37.0 9 36.818366344773644 39.0 37.0 39.0 32.0 39.0 10-11 36.64719717539985 39.0 35.0 39.0 32.0 39.0 12-13 36.60043584433591 39.0 35.0 39.0 32.0 39.0 14-15 37.661773671469135 39.0 36.5 41.0 32.0 41.0 16-17 37.66479377941366 39.0 36.5 41.0 32.0 41.0 18-19 37.66334142787626 39.0 37.0 41.0 32.0 41.0 20-21 37.6408101522326 39.0 37.0 41.0 32.0 41.0 22-23 37.552375789242056 39.0 36.0 41.0 32.0 41.0 24-25 37.434755019561635 39.0 36.0 41.0 32.0 41.0 26-27 37.24285163674229 39.0 36.0 40.5 31.0 41.0 28-29 37.085938661771735 39.0 36.0 40.0 31.0 41.0 30-31 36.91805302756083 39.0 36.0 40.0 30.5 41.0 32-33 37.099158953842675 39.0 36.0 40.0 31.0 41.0 34-35 37.0678905388868 39.0 36.0 40.0 31.0 41.0 36-37 36.9498012789055 39.0 35.5 40.0 30.5 41.0 38-39 36.7437632851325 39.0 35.0 40.0 30.0 41.0 40-41 36.749628710215276 39.0 35.0 40.0 30.0 41.0 42-43 36.63757390395804 38.0 35.0 40.0 30.0 41.0 44-45 36.69947564832022 38.5 35.0 40.0 30.0 41.0 46-47 36.84698251099954 39.0 35.0 40.0 30.0 41.0 48-49 36.781297405251294 39.0 35.0 40.0 30.0 41.0 50-51 36.62831135756165 39.0 35.0 40.0 30.0 41.0 52-53 36.50007979288873 38.5 35.0 40.0 30.0 41.0 54-55 36.35023248843187 38.0 35.0 40.0 30.0 41.0 56-57 36.17638362789113 38.0 34.0 40.0 29.0 41.0 58-59 36.033102281534624 38.0 34.0 40.0 29.0 41.0 60-61 35.81807464688032 38.0 34.0 40.0 29.0 41.0 62-63 35.56802000957663 37.0 34.0 40.0 28.0 41.0 64-65 35.348479390610144 37.0 34.0 39.5 28.0 41.0 66-67 35.07804688623092 36.0 33.0 39.0 28.0 41.0 68-69 34.75468959277397 36.0 33.0 39.0 28.0 40.0 70-71 34.41221648390373 35.5 33.0 38.5 28.0 40.0 72-73 34.11567098252698 35.0 32.0 38.0 27.0 40.0 74-75 33.75636105808397 35.0 32.0 37.0 26.5 39.0 76-77 32.70353035471129 34.0 31.0 36.0 26.0 39.0 78-79 33.07085291095147 34.0 31.0 36.0 26.0 39.0 80-81 32.95975453987549 34.0 31.0 36.0 26.0 37.5 82-83 32.515694134954245 34.0 31.0 35.5 26.0 37.0 84-85 32.27117033379494 34.0 31.0 35.0 26.0 37.0 86-87 32.18584861315797 34.0 31.0 35.0 26.0 36.0 88-89 32.02466168425977 34.0 31.0 35.0 26.0 36.0 90-91 31.89776576562096 34.0 31.0 35.0 26.0 35.5 92-93 31.810702465421347 34.0 31.0 35.0 25.5 35.0 94-95 31.77048114939948 34.0 31.0 35.0 25.0 35.0 96-97 31.69443778313926 34.0 31.0 35.0 25.0 35.0 98-99 31.934269934414836 34.0 31.0 35.0 26.0 35.0 100-101 31.065877836123907 33.0 29.0 34.5 24.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 0.0 15 0.0 16 1.0 17 0.0 18 6.0 19 24.0 20 102.0 21 589.0 22 2399.0 23 7715.0 24 18785.0 25 38510.0 26 66827.0 27 103208.0 28 145566.0 29 191355.0 30 239216.0 31 292455.0 32 358823.0 33 444720.0 34 563405.0 35 728901.0 36 995701.0 37 1296345.0 38 1142520.0 39 550091.0 40 13471.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.112456282246704 22.714872479702073 14.963928687289743 25.208742550761475 2 14.145298741727853 22.890959560974064 40.169925901319154 22.79381579597893 3 16.61641254449545 27.990902596623457 32.328778613741704 23.063906245139385 4 11.745438244090604 19.848123858450027 38.46751220986299 29.93892568759638 5 11.9834972425041 39.059229500984344 36.31185756567106 12.645415690840492 6 28.7007467014483 39.46071623789568 18.660689685054415 13.177847375601607 7 24.947908102727276 33.49228467756629 23.578214782488903 17.98159243721753 8 24.575418401674494 36.680694862302964 21.602347315607737 17.1415394204148 9 25.51096165725281 15.507831421676896 20.78844440346098 38.19276251760931 10-11 23.66414349866458 26.857351804037812 29.473841285113078 20.00466341218453 12-13 24.667575647822666 24.468804300004887 29.054571643787526 21.80904840838492 14-15 21.870187991894163 25.784037909458146 26.49260436710914 25.853169731538557 16-17 21.55174359263151 28.74506701526553 27.304305293609154 22.398884098493806 18-19 21.41902861277416 27.88126872232013 28.888854169704597 21.810848495201114 20-21 22.356079550070866 27.085668612665266 28.574687389109677 21.983564448154194 22-23 21.826352246409346 27.775846098622413 28.250578438737726 22.147223216230515 24-25 21.786461783661863 27.866128961100184 28.650135080633255 21.697274174604704 26-27 21.869064869729094 27.946096043750472 28.493769470946383 21.691069615574047 28-29 21.144808251715308 27.82272185948189 28.732729050890608 22.29974083791219 30-31 21.97693020757039 27.425878750361488 28.93693334842394 21.660257693644187 32-33 21.409527778180344 28.245941726139755 28.48380607603916 21.860724419640743 34-35 21.503858257002246 28.257073342115213 29.04292320154202 21.19614519934052 36-37 22.033204467588615 27.89910146348738 28.397809128621287 21.669884940302722 38-39 21.560660738106144 27.5912428133271 28.785034862245794 22.06306158632096 40-41 21.684066660384705 27.47917671229662 28.90582629222007 21.9309303350986 42-43 21.96911858191042 27.457453168805745 28.693309011360224 21.880119237923612 44-45 21.579585418053327 28.166334664925202 28.28134279667751 21.97273712034396 46-47 22.20070419860953 27.485489954233167 27.66403073198073 22.649775115176574 48-49 21.96816650700294 27.54329800757274 27.919240063461892 22.56929542196243 50-51 21.791474370932303 27.73139467893927 27.909400093813453 22.567730856314974 52-53 21.80601949672172 27.890521754668306 28.2173657669534 22.08609298165657 54-55 21.833298974377062 27.915076958128783 27.720938067468907 22.530686000025245 56-57 22.308111161853724 28.15464853620294 27.195069433110202 22.342170868833136 58-59 21.774264998141128 28.450139106779034 27.987373610769247 21.788222284310592 60-61 22.223814269345066 28.914099983434905 27.08253832146454 21.779547425755496 62-63 21.91466783811736 28.911168175807138 27.090222805304965 22.083941180770534 64-65 21.898690376829627 28.582128186280677 27.269319605194664 22.249861831695032 66-67 22.024731741116874 28.375487008589555 27.212330318258747 22.38745093203482 68-69 22.15683720985692 28.058414461424615 27.369976141317327 22.41477218740114 70-71 22.17363314847127 27.715257703868424 27.486113494170354 22.62499565348995 72-73 22.164658781210413 27.804598343633753 27.526425455623748 22.504317419532086 74-75 22.15044219310089 27.890690441928555 27.41674219659524 22.54212516837531 76-77 22.166123449118345 27.85646016685816 27.431934470587017 22.545481913436483 78-79 22.048037514088005 27.862912109516564 27.461589967059368 22.627460409336063 80-81 22.14980261073679 27.69671321508551 27.56380168121652 22.589682492961177 82-83 22.240445747719985 27.69358881198647 27.59572504436348 22.47024039593007 84-85 22.433722812948343 27.666578398087776 27.418767656460446 22.480931132503432 86-87 22.083731978415543 27.81042817346009 27.5387018011981 22.567138046926264 88-89 22.25150788051605 27.625686662221398 27.504851161168226 22.617954296094325 90-91 22.26116060174019 27.505887705495656 27.66306677672358 22.569884916040575 92-93 22.32234898459226 27.554273561735194 27.681149915727183 22.44222753794536 94-95 21.872749795188668 27.533284688006177 27.862867743239704 22.73109777356545 96-97 21.742861586404803 27.64085733823698 27.970531481986356 22.64574959337186 98-99 21.833527108945855 28.336389925596688 27.9899477748845 21.840135190572962 100-101 24.609524203778566 36.16558009782282 29.264531169356594 9.960364529042023 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1407.0 1 1515.0 2 2835.5 3 4220.0 4 5588.0 5 8427.5 6 10517.0 7 11950.0 8 12681.5 9 12025.0 10 10943.0 11 10308.0 12 11949.0 13 16745.5 14 27217.5 15 40971.0 16 50580.5 17 53580.5 18 52158.0 19 49164.5 20 45509.5 21 42409.5 22 41487.0 23 44185.0 24 51300.5 25 62878.5 26 78150.5 27 93427.0 28 108076.0 29 124842.5 30 140655.5 31 155720.5 32 173373.5 33 190744.5 34 208535.5 35 227538.0 36 245103.0 37 261052.5 38 270260.0 39 272708.0 40 278238.0 41 283589.0 42 287194.5 43 296216.5 44 306416.5 45 319154.0 46 342549.0 47 367010.0 48 368647.0 49 358042.0 50 350076.5 51 337663.0 52 317305.5 53 290818.0 54 266826.5 55 245288.5 56 222990.5 57 198123.0 58 172378.5 59 145622.0 60 118651.5 61 94551.0 62 73729.0 63 55663.0 64 40654.5 65 29808.0 66 21792.5 67 16574.5 68 12690.0 69 9220.0 70 6720.5 71 4604.5 72 3186.5 73 2221.0 74 1534.0 75 1060.5 76 721.5 77 507.0 78 369.5 79 263.0 80 192.5 81 137.0 82 104.5 83 75.5 84 50.5 85 33.5 86 23.5 87 19.0 88 14.0 89 8.5 90 6.5 91 6.0 92 5.0 93 3.5 94 2.5 95 1.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 9.443479111024206E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.001395690662732254 18-19 0.0014720717437773027 20-21 0.0 22-23 0.00268791756052659 24-25 1.6674449234257182E-4 26-27 0.0010983378741726058 28-29 4.105383382270895E-4 30-31 1.3946932340851034E-4 32-33 0.0010151576336773576 34-35 0.016715767621128787 36-37 0.0318237829122953 38-39 0.006570650525246756 40-41 0.003973897523135784 42-43 0.007514183112620457 44-45 0.005459208695541126 46-47 0.0029391377481207555 48-49 0.0012200497100760355 50-51 1.7265286861564027E-4 52-53 1.2746167327064184E-4 54-55 0.004417384950179826 56-57 0.011722612829530637 58-59 0.009488710491801543 60-61 0.008003395359076102 62-63 0.0018624180219109546 64-65 0.0012223975713369364 66-67 0.0017967360693215435 68-69 0.0 70-71 0.0 72-73 4.1655526235231667E-4 74-75 4.540659035200768E-4 76-77 3.0185265082973255E-5 78-79 4.8251642788776585E-4 80-81 0.0023924762916617483 82-83 0.004380325638561295 84-85 0.012031184478893835 86-87 0.00774953154643362 88-89 0.07047703118787067 90-91 0.0 92-93 0.0 94-95 8.855330839587832E-5 96-97 0.0071530701374422695 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 283.0 20-21 1172.0 22-23 1944.0 24-25 3475.0 26-27 5814.0 28-29 11762.0 30-31 26613.0 32-33 34773.0 34-35 48468.0 36-37 73834.0 38-39 88997.0 40-41 87254.0 42-43 94953.0 44-45 109551.0 46-47 109994.0 48-49 105912.0 50-51 96397.0 52-53 93808.0 54-55 106549.0 56-57 115887.0 58-59 117424.0 60-61 120609.0 62-63 113200.0 64-65 115863.0 66-67 122663.0 68-69 104567.0 70-71 103112.0 72-73 96721.0 74-75 95882.0 76-77 97249.0 78-79 104283.0 80-81 108546.0 82-83 103062.0 84-85 101882.0 86-87 106731.0 88-89 115950.0 90-91 128705.0 92-93 135632.0 94-95 170394.0 96-97 204822.0 98-99 552436.0 100-101 3063563.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.34789354502297 #Duplication Level Percentage of deduplicated Percentage of total 1 89.84966425804365 74.88780251635477 2 6.47958992407256 10.801203424140063 3 1.8401246148102384 4.601115315143405 4 0.7628592611711396 2.5433084995970803 5 0.39504849376896445 1.646322990188866 6 0.20390580796673807 1.0197071745374149 7 0.13446688778104174 0.7845272293673372 8 0.07923576342541383 0.5283307179952013 9 0.059382784212586436 0.44544869792619524 >10 0.1914403282585393 2.407882556592637 >50 0.003305946862558538 0.181225701487954 >100 9.172832799315963E-4 0.11607376178781573 >500 5.864634664150304E-5 0.03705141488130068 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.388746928091795E-5 0.0 0.0 0.0 0.0 3 1.388746928091795E-5 0.0 0.0 0.0 0.0 4 1.388746928091795E-5 0.0 0.0 0.0 0.0 5 4.1662407842753854E-5 0.0 0.0 0.0 0.0 6 8.332481568550771E-5 0.0 0.0 0.0 0.0 7 1.6664963137101542E-4 0.0 0.0 0.0 0.0 8 1.8053710065193336E-4 0.0 0.0 0.0 0.0 9 1.8053710065193336E-4 0.0 0.0 0.0 0.0 10-11 2.1525577385422823E-4 0.0 0.0 0.0 0.0 12-13 2.569181816969821E-4 0.0 0.0 0.0 0.0 14-15 3.402429973824898E-4 0.0 0.0 0.0 0.0 16-17 3.610742013038667E-4 0.0 0.0 0.0 0.0 18-19 4.930051594725872E-4 0.0 0.0 0.0 0.0 20-21 5.693862405176359E-4 0.0 0.0 0.0 0.0 22-23 5.83273709798554E-4 0.0 0.0 3.4718673202294876E-5 0.0 24-25 7.776982797314052E-4 0.0 0.0 9.026855032596668E-5 0.0 26-27 7.776982797314052E-4 0.0 0.0 1.5970589673055643E-4 0.0 28-29 8.818542993382899E-4 0.0 0.0 7.082609333268154E-4 0.0 30-31 9.304604418215027E-4 0.0 0.0 0.002291432431351462 0.0 32-33 9.512916457428797E-4 0.0 0.0 0.005318900734591575 0.0 34-35 0.0010901663385520593 0.0 0.0 0.009492085253507419 0.0 36-37 0.0012776471738444514 0.0 0.0 0.014949860680908174 0.0 38-39 0.0014026343973727131 0.0 0.0 0.024164196548797236 0.0 40-41 0.001409578132013172 0.0 0.0 0.03489226656830635 0.0 42-43 0.0016109464365864823 0.0 0.0 0.04424547712900459 0.0 44-45 0.0018470334143620875 0.0 0.0 0.05515408424916564 0.0 46-47 0.0020692329228567743 0.0 0.0 0.0672639574621261 0.0 48-49 0.002221995084946872 0.0 0.0 0.07865862600711927 0.0 50-51 0.002221995084946872 0.0 0.0 0.09031021273380943 0.0 52-53 0.002221995084946872 0.0 0.0 0.10137852575070103 0.0 54-55 0.00223588255422779 0.0 0.0 0.11318287463948129 0.0 56-57 0.0023955884509583468 0.0 0.0 0.12386233851650719 0.0 58-59 0.0024025321855988056 0.0 0.0 0.1349514827373202 0.0 60-61 0.0024303071241606414 0.0 0.0 0.1452559849437613 0.0 62-63 0.002465025797362936 0.0 0.0 0.15433838985348164 0.0 64-65 0.0024789132666438543 0.0 0.0 0.16480954169129378 0.0 66-67 0.0026108442248125746 0.0 0.0 0.1748779569199593 0.0 68-69 0.0026455628980148697 0.0 0.0 0.18571018295907532 0.0 70-71 0.0027080565097790006 0.0 0.0 0.19659795887531495 0.0 72-73 0.0027497189176217546 0.0 0.0 0.20535400825693373 0.0 74-75 0.0027636063869026723 0.0 0.0 0.21323514707385466 0.0 76-77 0.0027636063869026723 0.0 0.0 0.2205607871195389 0.0 78-79 0.002798325060104967 0.0 0.0 0.22724065984366043 0.0 80-81 0.003041355772521031 0.0 0.0 0.23136523822009308 0.0 82-83 0.003110793118925621 0.0 0.0 0.23427466303444539 0.0 84-85 0.0032149491385325057 0.0 0.0 0.23717020037951675 0.0 86-87 0.003298273954218013 0.0 0.0 0.23750349964225878 0.0 88-89 0.003305217688858472 0.0 0.0 0.23762848686578705 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTATAC 10335 0.0 45.449047 1 TATACAC 13450 0.0 36.978348 3 GGTATCA 11550 0.0 35.508663 1 TTATACA 14050 0.0 34.26337 2 GTATCAA 18580 0.0 30.039204 1 TACACAT 18370 0.0 26.947304 5 ACACATC 18000 0.0 26.332796 6 ATACACA 21130 0.0 25.04949 4 ACATCTC 19020 0.0 24.90015 8 ATCAACG 23080 0.0 23.591213 3 CACATCT 19960 0.0 23.571396 7 TATCAAC 23930 0.0 23.079239 2 CAACGCA 23655 0.0 22.968369 5 TCAACGC 23730 0.0 22.91219 4 AACGCAG 23910 0.0 22.641966 6 CATCTCC 21475 0.0 21.96291 9 TACATGG 26220 0.0 20.038591 2 ACGCAGA 27185 0.0 19.813982 7 CGCAGAG 27320 0.0 19.673304 8 GTACATG 27310 0.0 19.581087 1 >>END_MODULE