FastQCFastQC Report
Fri 27 May 2016
SRR2139859_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139859_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12082845
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141650.040.0664521
CTTATAC149800.034.744911
GTATCAA226450.030.5752431
TATACAC187700.030.342483
TTATACA202850.026.9363082
TCAACGC299900.022.5280724
ATCAACG300050.022.5035213
CAACGCA304500.022.1877485
TATCAAC310850.021.991382
TACACAT255800.021.8436245
AACGCAG323700.020.9086636
ATACACA299350.020.0247384
ACACATC264350.019.9905016
ACATCTC271200.019.323818
ACGCAGA356650.018.8763227
TACATGG349400.018.1267572
CACATCT287250.018.1052557
GTACATG356150.018.0071771
CGCAGAG377150.017.8502988
ACATGGG350550.017.4413193
Produced by FastQC (version 0.11.5)