##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139859_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12082845
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.489348741956054	31.0	31.0	34.0	30.0	34.0
2	31.704895246111327	31.0	31.0	34.0	30.0	34.0
3	31.715624010735883	31.0	31.0	34.0	30.0	34.0
4	35.49495520301717	37.0	35.0	37.0	33.0	37.0
5	35.2760532804981	37.0	35.0	37.0	33.0	37.0
6	35.27834371789094	37.0	35.0	37.0	32.0	37.0
7	35.15901900587155	37.0	35.0	37.0	32.0	37.0
8	35.12373964906444	37.0	35.0	37.0	32.0	37.0
9	36.65231872129453	39.0	35.0	39.0	32.0	39.0
10-11	36.457225098890206	38.0	35.0	39.0	32.0	39.0
12-13	36.40848223245436	38.0	35.0	39.0	32.0	39.0
14-15	37.44778055168298	39.0	36.0	41.0	32.0	41.0
16-17	37.437798093081554	39.0	36.0	40.5	32.0	41.0
18-19	37.412629600065216	39.0	36.0	40.0	32.0	41.0
20-21	37.379822918675345	39.0	36.0	40.0	32.0	41.0
22-23	37.284076297975744	39.0	36.0	40.0	31.5	41.0
24-25	37.16419014006084	39.0	36.0	40.0	31.0	41.0
26-27	36.963834894916744	39.0	36.0	40.0	31.0	41.0
28-29	36.79134308506735	39.0	36.0	40.0	30.0	41.0
30-31	36.63603695337739	38.0	35.0	40.0	30.0	41.0
32-33	36.81428951721569	39.0	35.5	40.0	30.0	41.0
34-35	36.757852228698425	38.0	35.0	40.0	30.0	41.0
36-37	36.633419945041425	38.0	35.0	40.0	30.0	41.0
38-39	36.41405984889911	38.0	35.0	40.0	30.0	41.0
40-41	36.388370948702516	38.0	35.0	40.0	30.0	41.0
42-43	36.2392747643612	38.0	35.0	40.0	30.0	41.0
44-45	36.30062760991055	38.0	35.0	40.0	30.0	41.0
46-47	36.475923718498805	38.0	35.0	40.0	30.0	41.0
48-49	36.40431199844099	38.0	35.0	40.0	30.0	41.0
50-51	36.248947468743765	38.0	35.0	40.0	29.5	41.0
52-53	36.096922109517195	38.0	34.0	40.0	29.0	41.0
54-55	35.93241165008205	38.0	34.0	40.0	28.5	41.0
56-57	35.72758533171874	38.0	34.0	40.0	28.0	41.0
58-59	35.55407882050721	37.5	34.0	40.0	28.0	41.0
60-61	35.32827757559476	37.0	33.5	40.0	28.0	41.0
62-63	35.055668261878594	37.0	33.0	39.5	28.0	41.0
64-65	34.80252327813899	36.0	33.0	39.0	28.0	40.0
66-67	34.511479201154955	36.0	32.5	39.0	27.0	40.0
68-69	34.16309752377789	35.5	32.0	38.5	26.0	40.0
70-71	33.796273480605905	35.0	32.0	38.0	26.0	40.0
72-73	33.50131764055234	35.0	31.0	37.0	26.0	39.5
74-75	33.135457506492216	34.5	31.0	37.0	26.0	39.0
76-77	32.13435226362077	34.0	30.0	36.0	25.5	38.0
78-79	32.51156562446367	34.0	31.0	36.0	26.0	38.0
80-81	32.40071941711358	34.0	31.0	35.5	26.0	37.0
82-83	31.96923766990912	34.0	30.5	35.0	25.5	37.0
84-85	31.71637139955005	34.0	30.0	35.0	25.0	36.0
86-87	31.630926042008895	34.0	30.0	35.0	25.0	36.0
88-89	31.476687272419895	34.0	30.0	35.0	25.0	36.0
90-91	31.35782907881792	34.0	30.0	35.0	25.0	35.0
92-93	31.274855263000106	33.0	30.0	35.0	25.0	35.0
94-95	31.242426770668786	33.0	30.0	35.0	25.0	35.0
96-97	31.188056597490817	33.0	30.0	34.0	25.0	35.0
98-99	31.484333590568873	33.0	30.0	34.0	25.0	35.0
100-101	30.634723564438193	32.0	28.0	34.0	22.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	3.0
19	35.0
20	229.0
21	1157.0
22	5077.0
23	15792.0
24	39123.0
25	78271.0
26	134681.0
27	206476.0
28	286241.0
29	372075.0
30	463041.0
31	563738.0
32	682606.0
33	842095.0
34	1059340.0
35	1362317.0
36	1789247.0
37	2065331.0
38	1499793.0
39	608524.0
40	7651.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.754038059745035	23.506392741113537	15.701335240169017	25.038233958972413
2	14.628487398525817	23.28720946294276	39.184422622890665	22.899880515640763
3	17.15165592209451	27.96080724365826	31.72743670882147	23.16010012542576
4	12.645498638772573	19.910021191201245	37.70891706382065	29.735563106205532
5	12.469836367180081	38.68087358564974	36.24875598420736	12.60053406296282
6	29.652561131091225	39.22007606652242	17.99565416919608	13.131708633190279
7	25.501345088842903	32.73634644820818	23.713305930846584	18.04900253210233
8	24.463807985619283	36.92761100552064	21.290184555044775	17.318396453815303
9	25.349799653972223	15.832935041374776	20.716718620490457	38.100546684162545
10-11	24.07188042220189	26.820947384494296	29.26988221730892	19.837289975994892
12-13	24.806227341325656	24.081236662392012	28.974339238813375	22.138196757468958
14-15	22.039730709116935	25.450417513425027	26.077504925371464	26.432346852086575
16-17	21.979251330905804	28.582791968437064	26.810735174129164	22.627221526527965
18-19	22.103773641512436	27.660002106318604	28.336651785187776	21.89957246698118
20-21	22.78453751755241	27.024884018883743	27.931623088385425	22.25895537517842
22-23	22.45229727290409	27.37163227358271	27.755408948489247	22.420661505023954
24-25	22.366476936087842	27.598640537908835	27.920971639616184	22.113910886387135
26-27	22.471957774709914	27.660371155640345	27.965219827831543	21.902451241818195
28-29	22.217005977338456	27.366009074303616	28.151817961999903	22.26516698635803
30-31	22.50740797063039	27.7970294346191	27.616023937785883	22.079538656964633
32-33	22.811942160109027	27.491383524833484	27.731589040619696	21.965085274437794
34-35	22.37322967478304	27.44417672324584	27.98280303075681	22.19979057121431
36-37	22.218583123265	27.65079946879934	27.91125819350863	22.21935921442703
38-39	22.223449465040883	27.325034938808223	28.122753652426496	22.3287619437244
40-41	22.3455712362174	27.20418586166044	28.2933756524491	22.15686724967306
42-43	22.53523773506015	27.292257474855948	27.88948731497218	22.283017475111727
44-45	22.293896203803637	27.65368916977222	27.61271038177734	22.439704244646798
46-47	22.720902293845178	27.244356142442864	27.329543412219447	22.70519815149251
48-49	22.562752531122918	27.162747940625398	27.37249836559727	22.902001162654408
50-51	22.22508080124298	27.330624758348637	27.564041396220524	22.880253044187864
52-53	22.40782670222862	27.46865058679501	27.54128832382754	22.582234387148826
54-55	22.49357010678113	27.35122005588232	27.291976174800464	22.863233662536082
56-57	22.720523736127397	27.570020234990285	27.12980687282837	22.579649156053954
58-59	22.401940313281003	27.809000520520044	27.429558719952308	22.359500446246642
60-61	22.693349525748957	28.091467502385598	26.784845215710853	22.4303377561546
62-63	22.54204151242154	28.085694791046915	26.828709494093346	22.543554202438195
64-65	22.442835305283857	27.857832303854668	27.011426214763773	22.6879061760977
66-67	22.57369089124547	27.80918719668494	26.84209530008337	22.77502661198622
68-69	22.639886598214694	27.634355705374187	26.978569453629742	22.747188242781377
70-71	22.625189260544932	27.220065019535205	27.152631461849687	23.00211425807018
72-73	22.65766852390401	27.411471324968883	27.017069298390634	22.913790852736472
74-75	22.40661799078423	27.537853660708322	27.153857712085124	22.901670636422324
76-77	22.571295432044383	27.57032238143867	27.000389503517567	22.857992682999377
78-79	22.65183895263266	27.59776786554365	26.893221133215643	22.857172048608042
80-81	22.596438739900247	27.46688772646172	26.99384388380644	22.942829649831598
82-83	22.721151724102633	27.391028530257717	27.104530656350384	22.78328908928927
84-85	22.75818545588135	27.304788106910248	26.99639789006269	22.94062854714571
86-87	22.444412361496816	27.509793948841537	27.22458912595671	22.82120456370494
88-89	22.588628401783723	27.310420784513234	27.159278423608658	22.941672390094382
90-91	22.549688492382177	27.337109561048344	27.254267222644952	22.858934723924527
92-93	22.6630732453041	27.325907401121253	27.237567603580036	22.773451749994607
94-95	22.2365517339069	27.406337109884916	27.361849029949877	22.99526212625831
96-97	22.087400161003018	27.402568328663207	27.54216623482952	22.967865275504256
98-99	22.19187620896003	28.015521896863486	27.587339164715075	22.20526272946141
100-101	25.19071371262062	36.086200478572636	28.579988704661336	10.143097104145408
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1905.0
1	1987.5
2	3386.5
3	4895.5
4	6320.5
5	9262.0
6	11459.5
7	13207.5
8	14398.0
9	14114.0
10	13131.5
11	12427.5
12	14211.5
13	19792.0
14	32313.0
15	49097.0
16	61185.5
17	65156.0
18	64311.0
19	61627.5
20	57895.0
21	55083.0
22	55329.0
23	61133.5
24	73493.0
25	91906.5
26	116081.5
27	141951.5
28	166372.0
29	190604.0
30	210963.0
31	231430.0
32	261570.0
33	293834.0
34	323566.5
35	357806.5
36	396460.5
37	422234.5
38	430733.0
39	437289.5
40	451236.0
41	463955.0
42	470734.0
43	483440.0
44	496952.0
45	509954.0
46	530255.0
47	551534.5
48	557485.0
49	556298.5
50	560618.5
51	558881.0
52	538244.0
53	508116.5
54	474816.5
55	437680.5
56	405679.0
57	377254.0
58	342633.5
59	294570.0
60	241029.5
61	190523.0
62	147310.0
63	112865.0
64	84776.0
65	62274.0
66	45174.0
67	33409.0
68	25204.5
69	18837.0
70	14078.5
71	10351.0
72	7563.0
73	5438.5
74	3872.0
75	2672.5
76	1819.0
77	1219.5
78	856.0
79	632.0
80	457.0
81	308.5
82	219.5
83	164.0
84	128.0
85	97.5
86	73.5
87	56.5
88	33.0
89	15.0
90	10.5
91	8.5
92	8.5
93	5.5
94	2.5
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	7.614100818143409E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0015766154411481733
18-19	0.0012621199725726846
20-21	8.276753104247982E-6
22-23	0.0023178881820111762
24-25	1.490527079357732E-4
26-27	0.0011019004385729445
28-29	4.7265229665482413E-4
30-31	1.6199749726328075E-4
32-33	8.836370467499434E-4
34-35	0.015487573567544983
36-37	0.028041081660479007
38-39	0.005377532363228766
40-41	0.0032953170189615813
42-43	0.006955126515447688
44-45	0.004812772366919248
46-47	0.0026835189873033387
48-49	0.0011589287424061308
50-51	1.5597785591554736E-4
52-53	1.1149294865169022E-4
54-55	0.004292373111897084
56-57	0.011160021431825496
58-59	0.009475447655498327
60-61	0.007470135227186719
62-63	0.0016829645420408047
64-65	0.0010442745406936723
66-67	0.0013535950083150149
68-69	0.0
70-71	0.0
72-73	1.2031440341146022E-4
74-75	2.782531268695132E-4
76-77	5.665505389142036E-6
78-79	2.770707662980817E-4
80-81	0.0019384976560676212
82-83	0.003667047943309968
84-85	0.010315109051369797
86-87	0.006648906056807449
88-89	0.06435598880765411
90-91	0.0
92-93	0.0
94-95	4.270853849744101E-5
96-97	0.006115372344862334
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	480.0
20-21	1733.0
22-23	3311.0
24-25	5398.0
26-27	8841.0
28-29	16925.0
30-31	38386.0
32-33	52609.0
34-35	72146.0
36-37	108694.0
38-39	122075.0
40-41	124934.0
42-43	134627.0
44-45	154535.0
46-47	155610.0
48-49	148566.0
50-51	137018.0
52-53	136878.0
54-55	149699.0
56-57	159306.0
58-59	162044.0
60-61	166912.0
62-63	165457.0
64-65	168327.0
66-67	179149.0
68-69	163884.0
70-71	163291.0
72-73	157914.0
74-75	159535.0
76-77	159891.0
78-79	173524.0
80-81	183167.0
82-83	175304.0
84-85	177708.0
86-87	189440.0
88-89	209327.0
90-91	236008.0
92-93	260136.0
94-95	335177.0
96-97	408519.0
98-99	1019395.0
100-101	5336965.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.64560992929592
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.47471158141511	73.94692111893889
2	6.655259884885875	11.000560248487377
3	1.9295280388089926	4.784010649291421
4	0.8163674279165809	2.698767360263054
5	0.3914589887517497	1.6176183443846872
6	0.23367171646483764	1.1587164918277204
7	0.13622091145844983	0.7880642218825739
8	0.09181526807837559	0.6070503064927332
9	0.06355129966847624	0.4727012330610612
>10	0.2033903945455357	2.500883770586489
>50	0.003067697957999322	0.16587690761957347
>100	8.46214274491391E-4	0.12635020365068633
>500	3.685874219336503E-5	0.02360815218264455
>1k	7.371703130339455E-5	0.1088709913310743
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	4.138098270730114E-6	0.0
14-15	0.0	0.0	0.0	8.276196541460227E-6	0.0
16-17	8.276196541460227E-6	0.0	0.0	8.276196541460227E-6	0.0
18-19	8.276196541460227E-6	0.0	0.0	8.276196541460227E-6	0.0
20-21	8.276196541460227E-6	0.0	0.0	2.8966687895110794E-5	0.0
22-23	8.276196541460227E-6	0.0	0.0	8.276196541460228E-5	0.0
24-25	8.276196541460227E-6	0.0	0.0	2.8552878068037783E-4	0.0
26-27	8.276196541460227E-6	0.0	0.0	6.000242492558665E-4	0.0
28-29	8.276196541460227E-6	0.0	0.0	0.0032359928477109483	0.0
30-31	8.276196541460227E-6	0.0	0.0	0.008127225003713944	0.0
32-33	8.276196541460227E-6	0.0	0.0	0.017591055748873714	0.0
34-35	8.276196541460227E-6	0.0	0.0	0.032662009650872785	0.0
36-37	8.276196541460227E-6	0.0	0.0	0.049694422133197934	0.0
38-39	8.276196541460227E-6	0.0	0.0	0.07337262043831565	0.0
40-41	8.276196541460227E-6	0.0	0.0	0.09448519781558068	0.0
42-43	8.276196541460227E-6	0.0	0.0	0.11344596409206606	0.0
44-45	8.276196541460227E-6	0.0	0.0	0.13361505506360466	0.0
46-47	8.276196541460227E-6	0.0	0.0	0.15450831323252098	0.0
48-49	1.2414294812190341E-5	0.0	4.138098270730114E-6	0.17068000127453425	0.0
50-51	1.6552393082920455E-5	0.0	8.276196541460227E-6	0.18528748817021157	0.0
52-53	1.6552393082920455E-5	0.0	8.276196541460227E-6	0.19803696894233105	0.0
54-55	1.6552393082920455E-5	0.0	8.276196541460227E-6	0.21163062176167946	4.138098270730114E-6
56-57	1.6552393082920455E-5	0.0	8.276196541460227E-6	0.223382820850553	8.276196541460227E-6
58-59	1.6552393082920455E-5	0.0	8.276196541460227E-6	0.2338563475737709	8.276196541460227E-6
60-61	1.6552393082920455E-5	0.0	8.276196541460227E-6	0.24341121648088676	8.276196541460227E-6
62-63	1.6552393082920455E-5	0.0	8.276196541460227E-6	0.25261848513326124	8.276196541460227E-6
64-65	1.6552393082920455E-5	0.0	8.276196541460227E-6	0.2620740396818796	8.276196541460227E-6
66-67	1.6552393082920455E-5	0.0	8.276196541460227E-6	0.271819261109449	8.276196541460227E-6
68-69	1.6552393082920455E-5	0.0	8.276196541460227E-6	0.2820527781329646	8.276196541460227E-6
70-71	1.6552393082920455E-5	0.0	8.276196541460227E-6	0.2921621522083582	8.276196541460227E-6
72-73	1.6552393082920455E-5	0.0	8.276196541460227E-6	0.29989211977808206	8.276196541460227E-6
74-75	1.6552393082920455E-5	0.0	8.276196541460227E-6	0.3073158680757719	8.276196541460227E-6
76-77	1.6552393082920455E-5	0.0	8.276196541460227E-6	0.31427614936713993	8.276196541460227E-6
78-79	1.6552393082920455E-5	0.0	8.276196541460227E-6	0.32069847788331307	8.276196541460227E-6
80-81	1.6552393082920455E-5	0.0	8.276196541460227E-6	0.3250021000848724	8.276196541460227E-6
82-83	1.6552393082920455E-5	0.0	8.276196541460227E-6	0.32803118801904685	8.276196541460227E-6
84-85	1.6552393082920455E-5	0.0	8.276196541460227E-6	0.3309485472999115	8.276196541460227E-6
86-87	1.6552393082920455E-5	0.0	8.276196541460227E-6	0.33145339528894063	8.276196541460227E-6
88-89	1.6552393082920455E-5	0.0	8.276196541460227E-6	0.33156098584397964	8.276196541460227E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14165	0.0	40.066452	1
CTTATAC	14980	0.0	34.74491	1
GTATCAA	22645	0.0	30.575243	1
TATACAC	18770	0.0	30.34248	3
TTATACA	20285	0.0	26.936308	2
TCAACGC	29990	0.0	22.528072	4
ATCAACG	30005	0.0	22.503521	3
CAACGCA	30450	0.0	22.187748	5
TATCAAC	31085	0.0	21.99138	2
TACACAT	25580	0.0	21.843624	5
AACGCAG	32370	0.0	20.908663	6
ATACACA	29935	0.0	20.024738	4
ACACATC	26435	0.0	19.990501	6
ACATCTC	27120	0.0	19.32381	8
ACGCAGA	35665	0.0	18.876322	7
TACATGG	34940	0.0	18.126757	2
CACATCT	28725	0.0	18.105255	7
GTACATG	35615	0.0	18.007177	1
CGCAGAG	37715	0.0	17.850298	8
ACATGGG	35055	0.0	17.441319	3
>>END_MODULE