FastQCFastQC Report
Fri 27 May 2016
SRR2139858_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139858_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32865586
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA485950.053.9965861
GTATCAA912300.037.1856161
ATCAACG1132350.029.4418243
TCAACGC1132100.029.4372374
TATCAAC1159100.028.9212472
CAACGCA1151750.028.8841295
AACGCAG1188650.028.0711886
ACGCAGA1386150.023.9436957
CGCAGAG1407050.023.5999228
GCAGAGT1606600.020.4891599
GAGTACT923000.019.67271612-13
GTACATG1155400.018.823331
TACATGG1134400.018.7290212
CAGAGTA1581650.018.377310-11
ACATGGG1149150.018.0296843
GTACTTT1002000.018.01533914-15
AGAGTAC1475150.017.75618710-11
GTGGTAT201800.017.1738951
AGTACTT1015000.016.56159812-13
TGGTATC188100.016.5333022
Produced by FastQC (version 0.11.5)