##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139858_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32865586
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.892614420445753	31.0	30.0	33.0	28.0	34.0
2	31.320021830738085	31.0	31.0	34.0	28.0	34.0
3	31.471688227314736	31.0	31.0	34.0	28.0	34.0
4	35.15081407646284	37.0	35.0	37.0	32.0	37.0
5	35.199086241760604	37.0	35.0	37.0	33.0	37.0
6	35.069740092265505	37.0	35.0	37.0	32.0	37.0
7	35.087404222763595	37.0	35.0	37.0	32.0	37.0
8	35.18587175046871	37.0	35.0	37.0	32.0	37.0
9	36.90222696774675	39.0	37.0	39.0	33.0	39.0
10-11	36.879230922582664	39.0	37.0	39.0	32.5	39.0
12-13	36.79318972130909	39.0	37.0	39.0	32.0	39.0
14-15	37.96831920173278	40.0	37.0	41.0	33.0	41.0
16-17	38.00825255329389	40.0	37.0	41.0	33.0	41.0
18-19	38.061404062596054	40.0	37.5	41.0	33.0	41.0
20-21	38.07377788189445	40.0	37.5	41.0	33.0	41.0
22-23	38.021271459908235	40.0	37.0	41.0	33.0	41.0
24-25	38.046480420660934	40.0	37.0	41.0	33.0	41.0
26-27	37.932904396762254	40.0	37.0	41.0	32.5	41.0
28-29	37.88934599661639	40.0	37.0	41.0	32.5	41.0
30-31	37.81293991428347	40.0	37.0	41.0	32.0	41.0
32-33	37.7233921023563	40.0	37.0	41.0	32.0	41.0
34-35	37.6412934908301	40.0	37.0	41.0	32.0	41.0
36-37	37.54572460199814	40.0	37.0	41.0	32.0	41.0
38-39	37.519716975404165	40.0	37.0	41.0	31.0	41.0
40-41	37.44829646565272	40.0	36.0	41.0	31.0	41.0
42-43	37.39812081765085	40.0	36.0	41.0	31.0	41.0
44-45	37.33317810787189	39.0	36.0	41.0	31.0	41.0
46-47	37.205900688725535	39.0	36.0	41.0	31.0	41.0
48-49	37.13400252701027	39.0	36.0	41.0	31.0	41.0
50-51	37.0675290039243	39.0	36.0	41.0	31.0	41.0
52-53	36.986454316965606	39.0	35.5	41.0	30.5	41.0
54-55	36.83657402342577	39.0	35.0	40.5	30.0	41.0
56-57	36.68595517569385	39.0	35.0	40.0	30.0	41.0
58-59	36.50494878747231	38.0	35.0	40.0	30.0	41.0
60-61	36.330243346612804	38.0	35.0	40.0	30.0	41.0
62-63	36.14305556309492	38.0	34.0	40.0	29.0	41.0
64-65	35.955576506704546	37.5	34.0	40.0	29.0	41.0
66-67	35.697141725960634	37.0	34.0	40.0	29.0	41.0
68-69	35.39502734165925	36.5	34.0	39.0	29.0	41.0
70-71	35.10714291129766	36.0	34.0	39.0	29.0	40.5
72-73	34.83580428911765	36.0	33.5	39.0	29.0	40.0
74-75	34.53232422124823	35.0	33.0	38.0	28.5	40.0
76-77	34.23537250869349	35.0	33.0	37.0	28.0	39.0
78-79	34.196593416762525	35.0	33.0	37.0	29.0	39.0
80-81	33.955551350143274	35.0	33.0	36.5	29.0	39.0
82-83	33.75393967428425	35.0	33.0	36.0	29.0	38.5
84-85	33.53819647401333	35.0	33.0	36.0	29.0	37.0
86-87	33.33384243589387	35.0	33.0	35.5	29.0	37.0
88-89	33.18054698830892	35.0	33.0	35.0	29.0	36.5
90-91	33.06881268684471	35.0	32.5	35.0	29.0	36.0
92-93	33.00662064311973	35.0	32.0	35.0	29.0	36.0
94-95	32.967990695777914	34.5	32.0	35.0	29.0	36.0
96-97	33.01208411533013	34.0	32.0	35.0	29.0	35.0
98-99	33.12022566536295	34.0	33.0	35.0	29.0	35.0
100	32.6779901633125	34.0	32.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	3.0
16	25.0
17	344.0
18	3216.0
19	11714.0
20	24913.0
21	42635.0
22	67587.0
23	104714.0
24	158254.0
25	228994.0
26	319181.0
27	421698.0
28	537676.0
29	665321.0
30	818161.0
31	1003755.0
32	1243173.0
33	1579038.0
34	2080760.0
35	2818578.0
36	4100098.0
37	6153841.0
38	6941242.0
39	3417471.0
40	123193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.91962680963607	19.212458283871765	9.62827499865665	29.239639907835507
2	14.277627668041578	23.111530705705356	36.91791468437532	25.69292694187774
3	17.489370188013687	27.577831717347134	29.230822173686484	25.701975920952698
4	12.052093031294193	19.449983335151853	38.26083307931889	30.237090554235056
5	12.998956385481689	39.04577573187471	34.63513698853664	13.320130894106965
6	28.297631059046395	40.188134293237866	18.357909399064848	13.15632524865089
7	25.313210602725906	34.63578285200818	22.836014547253168	17.214991998012756
8	24.82209810590324	36.895903818663086	20.68510203956199	17.596896035871687
9	25.944016333681073	16.977284384949044	21.039877396374433	36.03882188499545
10-11	23.549555757198426	27.62535711366899	28.809662788303854	20.015424340828734
12-13	24.689328527414663	25.402897121627465	29.045766900368065	20.862007450589807
14-15	22.078529194641472	26.319888225939437	26.983521608286555	24.61806097113254
16-17	21.16744396402973	29.304412828665217	27.652008091381663	21.876135115923386
18-19	21.13185658700867	28.402031231087737	29.588845608899227	20.87726657300436
20-21	22.165994782089903	27.468112099391096	29.45569168282433	20.910201435694674
22-23	21.802934550655497	27.34192556848732	29.39204871725743	21.463091163599753
24-25	21.43777550420132	27.513993534509535	29.522268197654398	21.525962763634745
26-27	21.446523546755053	27.688358308470708	29.350951810167086	21.514166334607157
28-29	21.49383369797433	27.594850535896526	29.449170495073762	21.462145271055377
30-31	21.72066548933748	27.52262395325452	29.346426749381223	21.410283808026772
32-33	21.49925256648317	27.724727200314746	29.313560945342545	21.462459287859538
34-35	21.66127439844176	27.682797800393917	29.31097529404575	21.344952507118574
36-37	21.63994399893121	27.55745032638731	29.29726528175981	21.50534039292167
38-39	21.46817156282484	27.781148391181056	29.329156816836647	21.421523229157458
40-41	21.755490210500945	27.645105574101013	29.111834524092945	21.4875696913051
42-43	21.601904452085567	27.741289335821083	28.93746156188668	21.719344650206672
44-45	21.66253146708868	27.977217726219884	28.73070826501431	21.629542541677132
46-47	21.827201653198593	27.916051889694494	28.394805530758866	21.86194092634805
48-49	21.754906037433845	28.04690513582949	28.340668731742564	21.8575200949941
50-51	21.763967711028872	28.234576805656058	28.09015812767685	21.91129735563822
52-53	21.958096040752977	28.190002479759634	27.847426544768723	22.004474934718665
54-55	21.899123846485715	28.087490285150608	27.989539684442168	22.02384618392151
56-57	21.885966562933664	28.249493511316633	27.92880581779556	21.935734107954143
58-59	21.891366910873998	28.277636811563873	27.891516072221023	21.939480205341102
60-61	21.979805569084398	28.231045455961613	27.88531173887252	21.90383723608147
62-63	21.946796041895915	28.33492596818225	27.882214717746507	21.836063272175327
64-65	21.905120970777283	28.186106179816917	27.90048104030819	22.00829180909761
66-67	21.942078853993177	28.34616713676113	27.73131694585784	21.98043706338785
68-69	21.879840982003394	28.25355591804819	27.853099294878536	22.013503805069885
70-71	21.915716278856006	27.980415530348267	27.90605969700258	22.197808493793147
72-73	21.936903846590226	28.084155930613026	27.860921187145205	22.118019035651546
74-75	21.762079229528013	28.263414766647983	27.903555559441866	22.070950444382138
76-77	21.94096428058075	28.193232754187825	27.801959585310243	22.063843379921178
78-79	21.850548476729642	28.243839985153645	27.795002961994538	22.110608576122175
80-81	21.885119281942217	28.299329838801963	27.721053438833742	22.094497440422078
82-83	21.938594311527222	28.207803398876912	27.851836796424323	22.00176549317154
84-85	21.994452083331243	28.22092475659428	27.792712669335085	21.9919104907394
86-87	21.799849177877583	28.340563381112265	27.88239117852355	21.977196262486594
88-89	21.868984957777386	28.239842372136266	27.81427983789939	22.076892832186957
90-91	21.875125430659352	28.282912846224217	27.867342055511184	21.974619667605243
92-93	21.92549908555893	28.296065645822654	27.81659012863488	21.961845139983545
94-95	21.74759705447426	28.326569593493982	27.89803138883713	22.02780196319463
96-97	21.797156283532573	28.471947200598613	27.958822928547512	21.7720735873213
98-99	22.710914472059727	30.454861964374007	28.1626175168327	18.671606046733572
100	21.953040285201325	31.268868576220797	21.99460776204849	24.78348337652939
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27069.0
1	21412.0
2	11912.0
3	7736.5
4	7954.5
5	11685.0
6	16848.0
7	21885.5
8	28440.5
9	33858.0
10	35418.0
11	38673.5
12	47131.5
13	70471.5
14	115799.5
15	173538.0
16	221672.5
17	244089.5
18	249461.5
19	232022.5
20	206284.0
21	190297.0
22	183737.0
23	198297.0
24	232812.5
25	284482.5
26	353566.5
27	427676.5
28	492513.0
29	561373.5
30	632879.5
31	699664.0
32	773463.5
33	848469.5
34	921701.5
35	986621.0
36	1057111.0
37	1127300.0
38	1173813.0
39	1206895.5
40	1236796.0
41	1259698.5
42	1273938.0
43	1295270.5
44	1314695.5
45	1329952.5
46	1337304.0
47	1333120.0
48	1327788.0
49	1312471.0
50	1287736.5
51	1249563.5
52	1190704.0
53	1118865.0
54	1046584.0
55	976891.5
56	908239.0
57	829522.5
58	746284.0
59	664324.0
60	567809.5
61	460928.0
62	359767.5
63	277703.0
64	213785.5
65	164197.5
66	125912.0
67	96495.0
68	73778.0
69	55823.5
70	41439.0
71	29720.0
72	21502.0
73	15796.5
74	11562.5
75	8404.5
76	5949.5
77	4147.5
78	2935.5
79	2101.5
80	1474.5
81	1064.0
82	810.0
83	571.5
84	403.0
85	295.5
86	226.5
87	182.0
88	143.0
89	109.5
90	81.0
91	61.5
92	49.5
93	40.5
94	39.0
95	49.0
96	50.0
97	37.0
98	20.0
99	17.0
100	32.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.1457847123127517
6	0.0062071006432077615
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.5220464316093246E-6
22-23	4.615201624221967E-4
24-25	4.039676636842674E-4
26-27	0.003292766289885501
28-29	6.630996185252065E-4
30-31	3.0620805600324876E-6
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0025783653215622707
48-49	0.0
50-51	2.3155411014334358E-5
52-53	0.0
54-55	1.011590176427565E-5
56-57	1.3270874021464448E-4
58-59	1.150828967010938E-4
60-61	1.5091989579588344E-4
62-63	0.0
64-65	6.716346064538465E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	2.3255798810177522E-4
76-77	5.2624049355945535E-5
78-79	3.21077238834539E-4
80-81	0.0
82-83	0.0
84-85	1.9687006520572803E-6
86-87	0.0
88-89	6.660629041916953E-4
90-91	0.0
92-93	1.4578387008108042E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	9279.0
20-21	23413.0
22-23	26598.0
24-25	29428.0
26-27	37925.0
28-29	60048.0
30-31	86679.0
32-33	103515.0
34-35	140975.0
36-37	200656.0
38-39	257411.0
40-41	257537.0
42-43	290708.0
44-45	344048.0
46-47	333693.0
48-49	309051.0
50-51	381762.0
52-53	252001.0
54-55	264175.0
56-57	277124.0
58-59	283433.0
60-61	283166.0
62-63	259275.0
64-65	263675.0
66-67	284163.0
68-69	292662.0
70-71	297737.0
72-73	274432.0
74-75	268115.0
76-77	280608.0
78-79	302457.0
80-81	316076.0
82-83	302363.0
84-85	297785.0
86-87	315115.0
88-89	355959.0
90-91	392964.0
92-93	420774.0
94-95	485747.0
96-97	1128001.0
98-99	2047376.0
100-101	2.0027677E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.24420712149856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.82122449624364	70.47144528140198
2	7.9873623262511	12.818791137242957
3	2.1209409025002572	5.105796632180662
4	0.8198844135624725	2.631638987903817
5	0.4248339368351853	1.7045231209822127
6	0.23290351754729688	1.121349486083452
7	0.14869708077014537	0.8352455543377222
8	0.09869699531941142	0.633588970774434
9	0.06639490358840514	0.47950257558239207
>10	0.2698766145397937	3.410043234299788
>50	0.006806404618784901	0.3756793265277646
>100	0.0022769957402907453	0.3072701671269032
>500	4.631043246166683E-5	0.024635692774431426
>1k	5.5102050718387364E-5	0.08048983278152873
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.0426963937292948E-5	0.0	6.08539278745859E-6	0.0
3	0.0	3.6512356724751536E-5	0.0	1.5213481968646474E-5	0.0
4	0.0	9.736628459933744E-5	0.0	1.8256178362375768E-5	0.0
5	0.0	9.736628459933744E-5	0.0	3.3469660331022244E-5	0.0
6	0.0	9.736628459933744E-5	0.0	4.259774951221013E-5	6.08539278745859E-6
7	0.0	9.736628459933744E-5	0.0	6.0853927874585896E-5	6.08539278745859E-6
8	0.0	1.1257976656798392E-4	0.0	6.693932066204449E-5	6.08539278745859E-6
9	3.042696393729295E-6	2.1298874756105063E-4	0.0	7.911010623696167E-5	6.08539278745859E-6
10-11	1.5213481968646474E-5	2.1298874756105063E-4	0.0	7.911010623696167E-5	6.08539278745859E-6
12-13	1.6734830165511122E-5	2.1298874756105063E-4	0.0	7.911010623696167E-5	6.08539278745859E-6
14-15	2.434157114983436E-5	2.7079997904190723E-4	0.0	7.911010623696167E-5	1.217078557491718E-5
16-17	2.434157114983436E-5	2.738426754356365E-4	0.0	8.063145443382632E-5	1.217078557491718E-5
18-19	2.5862919346699006E-5	3.2100446953844063E-4	0.0	8.519549902442026E-5	1.5213481968646474E-5
20-21	3.3469660331022244E-5	3.6816626364124464E-4	0.0	1.1257976656798392E-4	1.8256178362375768E-5
22-23	3.9555053118480836E-5	4.5184041446880033E-4	0.0	1.4300673050527687E-4	2.1298874756105064E-5
24-25	3.9555053118480836E-5	5.020449049653337E-4	0.0	1.764763908362991E-4	2.434157114983436E-5
26-27	4.259774951221013E-5	5.142156905402508E-4	0.0	2.525438006795315E-4	2.434157114983436E-5
28-29	5.4768535087127305E-5	5.142156905402508E-4	0.0	6.891707331796853E-4	2.5862919346699006E-5
30-31	5.4768535087127305E-5	5.750696184148367E-4	0.0	0.0017678066047567202	2.7384267543563652E-5
32-33	5.4768535087127305E-5	5.796336630054307E-4	0.0	0.0035995098337817557	2.7384267543563652E-5
34-35	5.78112314808566E-5	6.709145548173096E-4	0.0	0.005921087182197207	2.7384267543563652E-5
36-37	6.0853927874585896E-5	7.606740984323237E-4	0.0	0.009085491431675674	2.7384267543563652E-5
38-39	6.0853927874585896E-5	7.880583659758873E-4	0.0	0.014420859558079993	2.7384267543563652E-5
40-41	6.0853927874585896E-5	9.006381325438713E-4	0.0	0.020045283841888594	2.7384267543563652E-5
42-43	6.0853927874585896E-5	9.371504892686229E-4	0.0	0.024979016044320644	3.0426963937292948E-5
44-45	6.0853927874585896E-5	0.001083199916167629	0.0	0.03043000663368668	3.0426963937292948E-5
46-47	7.758875804009702E-5	0.00120795046831053	0.0	0.03611528484536987	3.3469660331022244E-5
48-49	9.432358820560813E-5	0.0012140358610979885	0.0	0.04144000353439613	3.6512356724751536E-5
50-51	9.432358820560813E-5	0.0014741864027618434	0.0	0.046627800885704576	3.6512356724751536E-5
52-53	9.432358820560813E-5	0.0014878785365336252	0.0	0.05219897798262292	4.4119097709074774E-5
54-55	1.0193032918993137E-4	0.001501570670305407	0.0	0.0577595056421632	4.716179410280407E-5
56-57	1.1410111476484855E-4	0.001536561678833294	0.0	0.06293513220789673	4.868314229966872E-5
58-59	1.1562246296171321E-4	0.0015396043752270233	0.0	0.06779127565228868	4.868314229966872E-5
60-61	1.1562246296171321E-4	0.0015517751608019404	0.0	0.07217275845925888	4.868314229966872E-5
62-63	1.277932485366304E-4	0.0015837234729360978	0.0	0.07649034464196075	6.0853927874585896E-5
64-65	1.277932485366304E-4	0.0016187144814639848	0.0	0.07987534437998459	6.0853927874585896E-5
66-67	1.2931459673349503E-4	0.001623278526054579	0.0	0.08310060255733764	6.541797246517984E-5
68-69	1.3083594493035967E-4	0.0016628335791730596	0.0	0.08599268547957734	6.998201705577378E-5
70-71	1.3692133771781826E-4	0.0016826111057323002	0.0	0.08832034822078025	6.998201705577378E-5
72-73	1.3996403411154756E-4	0.0016932605431103525	0.0	0.09054760198099009	7.911010623696167E-5
74-75	1.3996403411154756E-4	0.001726730203441375	0.0	0.09237626251362138	7.911010623696167E-5
76-77	1.3996403411154756E-4	0.001811925702465795	0.0	0.0939812848613136	7.911010623696167E-5
78-79	1.3996403411154756E-4	0.0018666942375529223	0.0	0.0950523139919063	7.911010623696167E-5
80-81	1.4300673050527687E-4	0.0018712582821435162	0.0	0.09543721508571307	8.823819541814955E-5
82-83	1.4300673050527687E-4	0.0018773436749309749	0.0	0.09589970493755992	9.888763279620208E-5
84-85	1.506134714896001E-4	0.0018834290677184336	0.0	0.09612182177430215	1.0040898099306673E-4
86-87	1.5822021247392334E-4	0.0019077706388682678	0.0	0.09619028244316107	1.0040898099306673E-4
88	1.5822021247392334E-4	0.002075118940523379	0.0	0.09619788918414539	1.0345167738679602E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	48595	0.0	53.996586	1
GTATCAA	91230	0.0	37.185616	1
ATCAACG	113235	0.0	29.441824	3
TCAACGC	113210	0.0	29.437237	4
TATCAAC	115910	0.0	28.921247	2
CAACGCA	115175	0.0	28.884129	5
AACGCAG	118865	0.0	28.071188	6
ACGCAGA	138615	0.0	23.943695	7
CGCAGAG	140705	0.0	23.599922	8
GCAGAGT	160660	0.0	20.489159	9
GAGTACT	92300	0.0	19.672716	12-13
GTACATG	115540	0.0	18.82333	1
TACATGG	113440	0.0	18.729021	2
CAGAGTA	158165	0.0	18.3773	10-11
ACATGGG	114915	0.0	18.029684	3
GTACTTT	100200	0.0	18.015339	14-15
AGAGTAC	147515	0.0	17.756187	10-11
GTGGTAT	20180	0.0	17.173895	1
AGTACTT	101500	0.0	16.561598	12-13
TGGTATC	18810	0.0	16.533302	2
>>END_MODULE